FastQCFastQC Report
Tue 24 May 2016
ERR841248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679775
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC519333.091664062151181No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA468672.7900760518521825No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT318531.896265868940781No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC123760.7367653405962108No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC49090.2922415204417258No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA47440.2824187763242101No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA46580.2772990430265958No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC40620.24181810063847836No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC36610.21794585584378862No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG34240.20383682338408418No Hit
ATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACG27610.16436725156643003No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG27010.16079534461460612No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC26900.16014049500677174No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC26750.15924751826881578No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG24810.14769835245791846No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC23260.13847092616570672No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC23060.13728029051509877No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTA22550.13424416960604843No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA22200.1321605572174845No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG22080.1314461758271197No Hit
ACGCAGCTGACTTTGTCTTCTCCGCACGACTGTTACAGAGGTCTCCAGA19860.11823012010537125No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG18990.11305085502522659No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG18620.11084817907160187No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG18480.11001473411617628No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC17140.10203747525710288No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT17070.10162075277939009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA950.0147.6277544
GCATTCA1250.0135.8175444
CTATCAT41250.0135.1017544
ACCACTC1500.0132.8649644
TAACTGG950.0132.0879844
GGGGATT1000.0125.4835844
AGCGCAC1550.0119.05463444
ATTAGTC5900.0117.6017644
TGTCGTT1350.0114.8215844
ACGTCTC1650.0107.3656344
AGACACG5900.0105.09093544
TGTCTAC850.0104.2078244
CACATCA2100.0101.9334444
ACTTCTA6900.097.3487344
AGTCTCC1600.096.88071444
CCTCGAA1250.094.4817644
TATTCAT1200.092.26733444
CGACGGG5050.089.1613144
TATAGGC4150.087.1537344
AGTTCGA1750.084.3587244
Produced by FastQC (version 0.11.5)