##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841248.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1679775 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.49613489901921 39.0 35.0 39.0 32.0 39.0 2 37.1767837954488 39.0 37.0 39.0 33.0 39.0 3 37.43669658138739 39.0 37.0 39.0 33.0 40.0 4 37.8562051465226 39.0 37.0 40.0 34.0 41.0 5 38.339966959860696 40.0 38.0 41.0 34.0 41.0 6 38.23860636097096 40.0 37.0 41.0 34.0 41.0 7 38.22030093316069 40.0 37.0 41.0 34.0 41.0 8 38.10479498742391 40.0 37.0 41.0 33.0 41.0 9 38.05732493935199 40.0 37.0 41.0 33.0 41.0 10 38.00445476328675 40.0 37.0 41.0 33.0 41.0 11 37.6429313449718 39.0 36.0 41.0 32.0 41.0 12 37.97016207527794 39.0 37.0 41.0 33.0 41.0 13 38.0684038040809 39.0 37.0 41.0 33.0 41.0 14 38.06339182330967 40.0 37.0 41.0 33.0 41.0 15 37.940131267580476 40.0 36.0 41.0 33.0 41.0 16 38.028708011489634 40.0 37.0 41.0 33.0 41.0 17 37.96869997469899 40.0 36.0 41.0 33.0 41.0 18 37.94081290649045 40.0 36.0 41.0 33.0 41.0 19 37.953446146061225 40.0 36.0 41.0 33.0 41.0 20 37.75209834650474 39.0 36.0 41.0 33.0 41.0 21 37.77824827729904 39.0 35.0 41.0 33.0 41.0 22 37.73277611584885 40.0 36.0 41.0 33.0 41.0 23 37.57440192882975 39.0 35.0 41.0 33.0 41.0 24 37.31692458811448 39.0 35.0 41.0 32.0 41.0 25 37.24842612512245 39.0 35.0 41.0 32.0 41.0 26 37.29857331079732 39.0 35.0 41.0 32.0 41.0 27 37.25351646684422 39.0 35.0 41.0 32.0 41.0 28 37.2586636479092 39.0 35.0 41.0 32.0 41.0 29 37.09995724832659 39.0 35.0 41.0 32.0 41.0 30 37.001207039775515 38.0 35.0 41.0 32.0 41.0 31 36.78158275446569 38.0 35.0 40.0 31.0 41.0 32 36.772451347236455 38.0 35.0 40.0 31.0 41.0 33 36.65657217444462 38.0 35.0 40.0 31.0 41.0 34 36.53474264194947 38.0 35.0 40.0 31.0 41.0 35 36.580753912846035 38.0 35.0 40.0 31.0 41.0 36 36.48557635088351 38.0 35.0 40.0 31.0 41.0 37 36.425751986162936 37.0 34.0 40.0 31.0 41.0 38 36.27640851069516 37.0 34.0 40.0 31.0 41.0 39 36.15549008339606 37.0 34.0 40.0 31.0 41.0 40 36.09751682610292 37.0 34.0 40.0 31.0 41.0 41 35.979865675083595 36.0 34.0 40.0 31.0 41.0 42 35.74187051058047 36.0 34.0 40.0 30.0 41.0 43 35.671788695805695 36.0 34.0 40.0 30.0 41.0 44 35.60781997728215 36.0 34.0 39.0 30.0 41.0 45 35.39514064577527 35.0 34.0 39.0 30.0 41.0 46 35.19356264490347 35.0 33.0 39.0 30.0 41.0 47 35.163198903317834 35.0 34.0 39.0 30.0 40.0 48 35.14316903560394 35.0 34.0 39.0 30.0 40.0 49 35.40171528277723 36.0 34.0 39.0 30.0 40.0 50 35.62715248476703 36.0 34.0 39.0 30.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 237.0 15 1778.0 16 813.0 17 3.0 18 0.0 19 1.0 20 2.0 21 13.0 22 33.0 23 86.0 24 220.0 25 577.0 26 1387.0 27 3051.0 28 6658.0 29 12835.0 30 24584.0 31 41694.0 32 66473.0 33 101750.0 34 145465.0 35 150511.0 36 182573.0 37 206247.0 38 233785.0 39 268205.0 40 230790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 39.520769150630294 19.99386822639937 40.48536262297034 2 28.247175961066212 19.141373100564063 29.398163444508935 23.213287493860786 3 29.287612924349986 21.033709871857837 25.079073090146004 24.599604113646173 4 25.425786191603045 23.62780729561995 28.864461014123915 22.081945498653095 5 25.18045571579527 25.021327261091514 24.787962673572352 25.010254349540862 6 22.407643880876904 25.741066511884032 21.07740620023515 30.773883407003915 7 29.311544700927207 22.05575151433972 19.511660787903143 29.12104299682993 8 25.880609010135284 22.908782426217797 24.25545087883794 26.955157684808977 9 29.626051108035302 24.627584051435463 23.74871634593919 21.99764849459005 10 36.55543153100862 19.234599872006665 20.76289979312706 23.44706880385766 11 24.827313181824948 18.7011951005343 27.805450134690656 28.666041582950097 12 28.532630858299473 21.469244392850236 22.113616406959267 27.884508341891028 13 27.915405337024303 22.5713562828355 23.255614591239897 26.257623788900297 14 22.074682621184387 21.70629995088628 20.915836942447648 35.30318048548169 15 23.864803321873467 30.582310130821096 23.456891547975175 22.09599499933027 16 21.53597952106681 31.682517003765387 18.181065916566208 28.600437558601598 17 22.10063847836764 33.60503638880207 18.421157595511303 25.873167537318984 18 24.067866232084654 22.67631081543659 21.052521915137444 32.20330103734131 19 30.114211724784568 20.31218466758941 20.153413403580835 29.420190204045184 20 23.037311544700927 20.75438674822521 22.9879001652007 33.220401541873166 21 24.99816939768719 28.040005357860426 21.09979015046658 25.862035093985803 22 26.692860650980045 20.251759908321056 23.443854087601018 29.611525353097885 23 29.28826777395782 22.184280632822848 26.307570954443303 22.219880638776026 24 28.17901207006891 22.45628134720424 25.337619621675522 24.02708696105133 25 24.431196096886346 25.09171932924838 22.6651188029427 27.81196577092257 26 25.259004826206944 29.772881532287432 21.11087451257004 23.857239128935586 27 23.800105653948588 24.804793057792224 24.21449579486317 27.18060549339602 28 23.765356201320543 20.547767114962777 20.950172717979413 34.73670396573726 29 23.775993605191083 23.625713120804104 25.782785614897136 26.815507659107684 30 23.71521101039964 24.313488326227777 25.29241381118128 26.6788868521913 31 23.764924964399167 29.94385707792396 23.298871979280378 22.992345978396493 32 28.630418116187357 22.5547853714277 21.35500463359653 27.45979187878842 33 24.216977329684276 20.447113810380323 26.97299157304271 28.362917286892685 34 24.190990965368215 27.69721928293064 25.876912595415654 22.23487715628549 35 23.23951289405759 22.061613786371787 21.048723643585856 33.65014967598477 36 22.314688115013077 22.264648694996914 28.22926865513634 27.191394534853675 37 21.990581417609928 30.056062359521164 21.863790260663805 26.089565962205107 38 28.431454428227337 26.117571598945684 20.882004135751533 24.568969837075443 39 25.642198245741838 26.909057551647013 24.26573152997105 23.183012672640103 40 24.0447615538786 24.34120605855477 24.655288772904985 26.95874361466164 41 26.62113675171441 27.898911784245577 21.450396139085175 24.029555324954842 42 22.837729151066366 29.353523587652887 22.554011703691376 25.254735557589374 43 25.8348956538066 23.700331520200873 22.518432579215954 27.946340246776575 44 25.89516969783558 22.51252947591922 20.453635634494358 31.138665191750846 45 24.37114680731125 21.40426434875551 29.511142420503393 24.71344642342984 46 22.612587815875013 26.19447065813848 30.436283247701933 20.756658278284576 47 22.23515681443471 31.803041811007578 23.25507122876568 22.706730145792037 48 27.9911501420099 26.50077657345943 20.02951531867148 25.478557965859196 49 20.47340243623147 27.130367447625954 21.080765766644724 31.31546434949785 50 19.63023030913935 27.06633153057686 31.062549403496533 22.24088875678726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2225.0 1 1670.5 2 1057.0 3 1122.0 4 1252.5 5 1019.5 6 755.0 7 796.5 8 915.5 9 1047.0 10 1150.0 11 1405.0 12 1914.0 13 2184.5 14 2182.5 15 2374.5 16 2496.0 17 2665.0 18 2865.5 19 3815.5 20 5614.0 21 7128.0 22 7226.5 23 8054.0 24 9985.0 25 11766.5 26 14440.5 27 17136.5 28 19669.5 29 24962.0 30 28000.5 31 28038.0 32 34315.0 33 40708.5 34 44657.5 35 45707.5 36 44098.0 37 49267.5 38 52845.5 39 53145.0 40 55247.0 41 55911.0 42 58880.5 43 58196.0 44 58400.0 45 72871.5 46 83451.0 47 86085.0 48 96223.0 49 114692.0 50 150188.5 51 163054.5 52 140478.0 53 123726.0 54 107491.0 55 98180.5 56 105635.0 57 107642.0 58 101220.5 59 99101.5 60 102556.0 61 103636.5 62 91450.0 63 80507.5 64 77364.5 65 70876.5 66 85682.0 67 106137.0 68 82865.0 69 56717.0 70 51891.0 71 46681.0 72 39237.5 73 32367.5 74 27961.5 75 23259.0 76 19972.5 77 16015.0 78 13126.5 79 11317.5 80 9453.0 81 7505.5 82 5761.0 83 4963.5 84 4265.0 85 3208.0 86 2418.5 87 1793.5 88 1280.0 89 981.5 90 559.0 91 265.0 92 118.0 93 45.5 94 47.0 95 40.0 96 31.0 97 31.0 98 296.5 99 562.0 100 562.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 13823.0 25 12894.0 26 11851.0 27 10514.0 28 14382.0 29 14044.0 30 13821.0 31 14075.0 32 13784.0 33 13540.0 34 14403.0 35 11843.0 36 11232.0 37 12864.0 38 14141.0 39 14161.0 40 11339.0 41 14784.0 42 14916.0 43 13498.0 44 14215.0 45 26323.0 46 23803.0 47 83064.0 48 292744.0 49 509428.0 50 464289.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.822588262038733 #Duplication Level Percentage of deduplicated Percentage of total 1 67.52994221143553 5.957888754907651 2 13.472448877993006 2.37723738663796 3 5.618424597502123 1.4870714071521578 4 2.932626520654349 1.0349342527227419 5 1.778522083611663 0.7845584029324464 6 1.1697427430729201 0.6192095156784081 7 0.8763075697945074 0.5411910615443297 8 0.6492105552747934 0.45821739396472333 9 0.4583710069347799 0.36396167988974976 >10 3.497076655913816 6.241838688249354 >50 0.5485240787831495 3.4247847449106636 >100 0.7731963029341811 16.799511160726723 >500 0.5707888937891075 36.82112362052383 >1k 0.12211913685086107 14.574636225286977 >5k 0.0 0.0 >10k+ 0.002698765455267648 8.513835704872292 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 51933 3.091664062151181 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA 46867 2.7900760518521825 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 31853 1.896265868940781 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 12376 0.7367653405962108 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 4909 0.2922415204417258 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 4744 0.2824187763242101 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 4658 0.2772990430265958 No Hit CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC 4062 0.24181810063847836 No Hit CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC 3661 0.21794585584378862 No Hit TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG 3424 0.20383682338408418 No Hit ATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACG 2761 0.16436725156643003 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 2701 0.16079534461460612 No Hit CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC 2690 0.16014049500677174 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 2675 0.15924751826881578 No Hit AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG 2481 0.14769835245791846 No Hit TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC 2326 0.13847092616570672 No Hit CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC 2306 0.13728029051509877 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTA 2255 0.13424416960604843 No Hit CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA 2220 0.1321605572174845 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 2208 0.1314461758271197 No Hit ACGCAGCTGACTTTGTCTTCTCCGCACGACTGTTACAGAGGTCTCCAGA 1986 0.11823012010537125 No Hit TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGG 1899 0.11305085502522659 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG 1862 0.11084817907160187 No Hit CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG 1848 0.11001473411617628 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 1714 0.10203747525710288 No Hit CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT 1707 0.10162075277939009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02101471923323064 0.0 0.0 0.0 0.0 2 0.04363679659478204 0.0 0.0 0.0 0.0 3 0.05709097944665208 0.0 0.0 0.0 0.0 4 0.07679599946421396 0.0 0.0 0.0 0.0 5 0.09632242413418464 0.0 0.0 0.0 0.0 6 0.13019600839398135 0.0 0.0 0.0 0.0 7 0.16317661591582205 0.0 0.0 0.0 0.0 8 0.22074384962271734 0.0 0.0 0.0 0.0 9 0.26420205086990817 0.0 0.0 0.0 0.0 10 0.3043264722953967 0.0 0.0 0.0 0.0 11 0.3459391882841452 0.0 0.0 0.0 0.0 12 0.39951779256150377 0.0 0.0 0.0 0.0 13 0.44654790076051853 0.0 0.0 0.0 0.0 14 0.49548302600050603 0.0 0.0 0.0 0.0 15 0.5489425667128038 0.0 0.0 0.0 0.0 16 0.6057358872468039 0.0 0.0 0.0 0.0 17 0.6642556294741855 0.0 0.0 0.0 0.0 18 0.7255733654804959 0.0 0.0 0.0 0.0 19 0.7834977898825736 0.0 0.0 0.0 0.0 20 0.8444583351937015 0.0 0.0 0.0 0.0 21 0.9105981455849742 0.0 0.0 0.0 0.0 22 0.9608429700406305 0.0 0.0 0.0 0.0 23 1.018172076617404 0.0 0.0 0.0 0.0 24 1.0722864669375363 0.0 0.0 0.0 0.0 25 1.1253292851721213 0.0 0.0 0.0 0.0 26 1.1849801312675805 0.0 0.0 0.0 0.0 27 1.2464169308389517 0.0 0.0 0.0 0.0 28 1.3108898513193732 0.0 0.0 0.0 0.0 29 1.3729219687160483 0.0 0.0 0.0 0.0 30 1.4365614442410441 0.0 0.0 0.0 0.0 31 1.4922236609069668 0.0 0.0 0.0 0.0 32 1.5419326993198494 0.0 0.0 0.0 0.0 33 1.6023574585882039 0.0 0.0 0.0 0.0 34 1.6727240255391347 0.0 0.0 0.0 0.0 35 1.7248738670357637 0.0 0.0 0.0 0.0 36 1.7867869208673781 0.0 0.0 0.0 0.0 37 1.843103987141135 0.0 0.0 0.0 0.0 38 1.8883481418642378 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGATA 95 0.0 147.62775 44 GCATTCA 125 0.0 135.81754 44 CTATCAT 4125 0.0 135.10175 44 ACCACTC 150 0.0 132.86496 44 TAACTGG 95 0.0 132.08798 44 GGGGATT 100 0.0 125.48358 44 AGCGCAC 155 0.0 119.054634 44 ATTAGTC 590 0.0 117.60176 44 TGTCGTT 135 0.0 114.82158 44 ACGTCTC 165 0.0 107.36563 44 AGACACG 590 0.0 105.090935 44 TGTCTAC 85 0.0 104.20782 44 CACATCA 210 0.0 101.93344 44 ACTTCTA 690 0.0 97.34873 44 AGTCTCC 160 0.0 96.880714 44 CCTCGAA 125 0.0 94.48176 44 TATTCAT 120 0.0 92.267334 44 CGACGGG 505 0.0 89.16131 44 TATAGGC 415 0.0 87.15373 44 AGTTCGA 175 0.0 84.35872 44 >>END_MODULE