FastQCFastQC Report
Tue 24 May 2016
ERR841247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350370
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC824412.460653599453195No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA636351.899342460683447No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT571631.7061697663243165No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA503841.5038339049119946No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC214930.6415112360724338No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA108360.32342696478299415No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG96400.2877294149601387No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC90100.26892552165880185No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC68340.203977471144978No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC66810.19941081134322475No Hit
ATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACG58490.17457773320558623No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC55850.16669800648883557No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG46040.13741765834818245No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG44840.13383596438602305No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC44560.1330002357948525No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC43530.12992594847733235No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC43010.12837388109372994No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA40150.1198375104839167No Hit
AATTCGTGGAGAAAGAAATGGCTCC39060.11658413846828858No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC37660.11240549551243594No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG36970.11034602148419427No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG35450.1058092091321257No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA35070.10467500604410857No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT34250.10222751516996631No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC33890.10115300698131849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC650.0123.5368644
AATCGCA12450.0109.64516444
TAAGCCA66050.0100.999144
CTATCAT73300.0100.44742644
AGACACG9800.088.2406144
CCTTAAG1550.087.6713244
CGGTAGG506.9667294E-1086.475844
AACGGTA1250.084.0050644
CCCGTGA1500.078.24000544
ACCACTC14200.077.4280344
CCCATAC15600.075.2307844
CCTAAGT2250.068.6315944
AGAACGT2100.067.6511344
TATAAGC1750.067.0628744
TAGTCGA1300.065.5688743
CCACTTT2900.063.89837344
GAGGTAA1550.063.7609644
GACATCA3250.062.7187144
TAGACGG3500.061.8220843
TACGGGA1600.061.7684344
Produced by FastQC (version 0.11.5)