##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841247.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3350370 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.72883621808935 39.0 37.0 39.0 32.0 39.0 2 37.323638881675755 39.0 37.0 39.0 34.0 39.0 3 37.52010703295457 39.0 37.0 39.0 34.0 39.0 4 37.92505544163779 39.0 37.0 40.0 34.0 41.0 5 38.44110859397619 40.0 38.0 41.0 34.0 41.0 6 38.350346379653594 40.0 38.0 41.0 34.0 41.0 7 38.309666395054876 40.0 38.0 41.0 34.0 41.0 8 38.195475126627805 40.0 37.0 41.0 34.0 41.0 9 38.103028322245 40.0 37.0 41.0 33.0 41.0 10 38.16123234150258 40.0 37.0 41.0 34.0 41.0 11 37.78773419055209 39.0 37.0 41.0 33.0 41.0 12 38.12210979682841 40.0 37.0 41.0 33.0 41.0 13 38.29755907556479 40.0 37.0 41.0 34.0 41.0 14 38.21780131746643 40.0 37.0 41.0 34.0 41.0 15 38.18946802890427 40.0 37.0 41.0 34.0 41.0 16 38.21670710996099 40.0 37.0 41.0 34.0 41.0 17 38.175733724931874 40.0 37.0 41.0 34.0 41.0 18 38.21842214441987 40.0 37.0 41.0 34.0 41.0 19 38.19261365162654 40.0 37.0 41.0 34.0 41.0 20 38.04623966905148 40.0 37.0 41.0 33.0 41.0 21 38.01967991594958 40.0 37.0 41.0 33.0 41.0 22 37.97112647259855 40.0 37.0 41.0 33.0 41.0 23 37.91145992830643 40.0 36.0 41.0 33.0 41.0 24 37.602173789760535 39.0 36.0 41.0 32.0 41.0 25 37.582198067073726 39.0 36.0 41.0 32.0 41.0 26 37.59137051531123 39.0 36.0 41.0 33.0 41.0 27 37.546631074722626 39.0 35.0 41.0 32.0 41.0 28 37.50013899796475 39.0 35.0 41.0 32.0 41.0 29 37.45322088911102 39.0 35.0 41.0 32.0 41.0 30 37.37031448161313 39.0 35.0 41.0 32.0 41.0 31 37.22406449165713 39.0 35.0 41.0 32.0 41.0 32 37.16115732270116 39.0 35.0 41.0 32.0 41.0 33 37.08670651136086 39.0 35.0 41.0 32.0 41.0 34 37.00186836841309 38.0 35.0 40.0 31.0 41.0 35 36.99888589915907 38.0 35.0 40.0 31.0 41.0 36 36.94552668369863 38.0 35.0 40.0 31.0 41.0 37 36.85320779591965 38.0 35.0 40.0 31.0 41.0 38 36.704143776670286 38.0 35.0 40.0 31.0 41.0 39 36.64254227515033 38.0 35.0 40.0 31.0 41.0 40 36.63039406812703 38.0 35.0 40.0 31.0 41.0 41 36.53344435107568 38.0 35.0 40.0 31.0 41.0 42 36.28165610299707 37.0 34.0 40.0 30.0 41.0 43 36.26651657642319 37.0 34.0 40.0 30.0 41.0 44 36.24653403481762 37.0 34.0 40.0 31.0 41.0 45 36.02090177702114 37.0 34.0 40.0 30.0 41.0 46 35.819963953171595 36.0 34.0 40.0 30.0 41.0 47 35.780553230060484 36.0 34.0 40.0 30.0 41.0 48 35.82190072627486 36.0 34.0 40.0 30.0 41.0 49 36.091353099220974 37.0 34.0 40.0 30.0 41.0 50 36.39178553234844 38.0 34.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 6.0 14 245.0 15 2281.0 16 2604.0 17 74.0 18 0.0 19 0.0 20 3.0 21 14.0 22 55.0 23 144.0 24 367.0 25 874.0 26 1997.0 27 4575.0 28 9681.0 29 20233.0 30 39057.0 31 69182.0 32 112646.0 33 175122.0 34 260164.0 35 273594.0 36 358208.0 37 405193.0 38 449931.0 39 580650.0 40 583470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 40.11282335980802 23.564919695436622 36.32225694475535 2 26.953769285183427 22.57867041550635 28.409011542008795 22.058548757301434 3 27.818778224494604 21.63268534520068 22.692926452899233 27.855609977405482 4 23.489763817130648 23.41072777036566 28.61498879228264 24.48451962022105 5 23.9374158675012 22.44122290970848 26.22540197052863 27.395959252261694 6 24.924679960720756 24.095458113581486 22.481576661682144 28.498285264015617 7 29.513814892086547 21.473538743482063 21.308213719678722 27.704432644752668 8 26.55175398538072 21.46025662837239 27.18410205439996 24.80388733184693 9 27.158164620623992 24.026689589508024 26.900431892596938 21.91471389727105 10 33.29148720887544 21.45873440843847 20.54614863432994 24.70362974835615 11 25.379316314317524 21.567946226834646 26.2454893041664 26.807248154681425 12 29.62607115035056 21.645251121517923 22.306730301429393 26.421947426702125 13 29.930813611630953 20.894050507854356 20.685178054961092 28.489957825553596 14 23.42690508809475 21.62871563439262 20.866441616896044 34.077937660616584 15 24.725985488169965 27.953748391968645 24.494936380160997 22.825329739700393 16 24.372323056856406 27.68031590540746 20.380286356432276 27.567074681303854 17 23.842739757101455 32.09902189907383 18.113223315633796 25.945015028190916 18 24.509203461110264 23.218092330100852 19.850643361777955 32.422060847010925 19 29.10033817160493 22.42680659151079 20.52400182666392 27.948853410220366 20 27.049430361422765 20.281670382674154 22.632455519838107 30.03644373606497 21 24.58376239042255 25.87991774042867 22.75596426663324 26.78035560251554 22 25.45118897315819 23.963592080874648 22.90803702277659 27.677181923190574 23 31.37044565227124 22.578252551210763 23.324200013729826 22.72710178278817 24 25.880066977677092 22.358008219987642 26.071836841901042 25.690087960434223 25 25.133512843537225 28.117316341491055 19.640975583946773 27.108195231024943 26 25.19354924719402 28.271733689809757 24.027345345425413 22.507371717570805 27 24.070970572529372 27.04797432152984 22.908288866302136 25.972766239638652 28 22.91068203207328 22.033039954757164 23.268474799807525 31.787803213362032 29 26.362343781363556 22.80011057035877 26.064914480156435 24.772631168121237 30 23.652221658007498 25.60037796827668 23.431813122077997 27.31558725163782 31 23.695929164363612 28.906978408714334 25.002498808927353 22.394593617994705 32 26.43433869610799 22.53931090510598 24.164816950335712 26.86153344845032 33 25.55659156736147 20.6279617631677 26.98754600079007 26.82790066868076 34 25.686194750955714 26.43312276554654 26.20157669391048 21.679105789587265 35 21.82840700169278 23.09887230224954 22.507992250412137 32.56472844564554 36 22.85793350190429 22.91691803674287 28.716484718448026 25.508663742904808 37 22.281844600365773 28.93679238581112 21.245979206695623 27.535383807127477 38 26.106276232893944 26.471716378990024 22.14138727957704 25.280620108538987 39 26.199144998848457 28.60952201636371 23.615279741381485 21.576053243406353 40 25.030835839416305 26.688376634604627 23.55508918490993 24.725698341069133 41 27.534791252485093 27.397539163643568 22.35016020714203 22.717509376729314 42 22.18815340620168 31.079355940792887 22.010573437470374 24.721917215535058 43 25.38558917621921 23.574784420953304 23.576875137856646 27.46275126497084 44 25.74450613822205 24.71138359261373 22.328429182290456 27.215681086873765 45 22.499195757013283 23.790824228069077 28.68738292214928 25.02259709276836 46 22.38659592010522 26.98287324614499 28.460757127728094 22.169773706021697 47 24.742502319458815 29.73253389503708 23.267698511237082 22.257265274267027 48 25.92764401865625 26.273075468587887 20.76998710353053 27.029293409225343 49 20.257093480998865 27.072206824323153 21.08211869947866 31.58858099519933 50 20.887671930718287 30.772505640055307 24.820791790990466 23.519030638235936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4085.0 1 3445.5 2 2708.5 3 2614.5 4 2691.0 5 2949.5 6 3055.5 7 2763.0 8 2594.5 9 2409.5 10 2224.0 11 2340.5 12 2430.0 13 3014.5 14 3971.0 15 5015.5 16 5780.5 17 7683.0 18 9689.5 19 10041.5 20 10573.0 21 14074.0 22 17953.0 23 20587.5 24 23391.0 25 26836.5 26 30582.5 27 37053.0 28 43805.5 29 48796.0 30 55930.5 31 67585.5 32 75959.0 33 75519.5 34 83157.5 35 96021.0 36 97351.5 37 97938.5 38 104185.0 39 111241.5 40 119472.0 41 119027.0 42 120105.5 43 162728.0 44 198120.5 45 176906.0 46 166304.5 47 171075.5 48 175604.0 49 211039.0 50 275107.5 51 298318.0 52 255089.0 53 212905.5 54 195736.5 55 184961.5 56 183843.5 57 193336.0 58 195945.0 59 189779.0 60 184506.5 61 177146.0 62 164208.0 63 157301.0 64 153496.5 65 146852.0 66 170155.5 67 195099.0 68 155872.0 69 116928.5 70 110042.0 71 96455.0 72 78179.5 73 66628.0 74 60328.0 75 50814.0 76 44715.5 77 39636.0 78 32308.0 79 25214.0 80 20132.0 81 15790.0 82 12195.0 83 10495.0 84 8823.5 85 6298.5 86 3824.5 87 2482.5 88 1512.5 89 1177.0 90 878.5 91 512.5 92 397.0 93 400.0 94 537.0 95 515.5 96 256.5 97 43.0 98 416.0 99 789.0 100 789.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 23911.0 25 26007.0 26 25179.0 27 27024.0 28 24962.0 29 25135.0 30 26641.0 31 22830.0 32 22376.0 33 26274.0 34 25803.0 35 24276.0 36 23497.0 37 25690.0 38 26500.0 39 24775.0 40 22030.0 41 26795.0 42 30836.0 43 27475.0 44 32258.0 45 38701.0 46 56322.0 47 136848.0 48 504349.0 49 974596.0 50 1099280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.439166249676836 #Duplication Level Percentage of deduplicated Percentage of total 1 67.71809482417459 5.7148426033258986 2 13.290268218096355 2.243175659906229 3 5.598054147847988 1.417287288851465 4 2.9336102958644408 0.9902889999425467 5 1.7845594031351104 0.7530096742740632 6 1.1349179489839771 0.5746656750730822 7 0.8095702746372351 0.47824686969221125 8 0.6242673942418412 0.421463705940756 9 0.49137437753880003 0.37321110565932647 >10 3.557021157026636 6.086227853148167 >50 0.5585447805284883 3.3292183634314383 >100 0.7903867024559129 16.31870230309969 >500 0.5656238022948608 34.96480722917594 >1k 0.13945918529673038 16.49081927561519 >5k 0.0021237439385288887 1.3023477758995678 >10k+ 0.0021237439385288887 8.541685616964454 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 82441 2.460653599453195 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA 63635 1.899342460683447 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 57163 1.7061697663243165 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 50384 1.5038339049119946 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 21493 0.6415112360724338 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 10836 0.32342696478299415 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG 9640 0.2877294149601387 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 9010 0.26892552165880185 No Hit ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC 6834 0.203977471144978 No Hit CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC 6681 0.19941081134322475 No Hit ATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACG 5849 0.17457773320558623 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 5585 0.16669800648883557 No Hit CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG 4604 0.13741765834818245 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 4484 0.13383596438602305 No Hit CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC 4456 0.1330002357948525 No Hit TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC 4353 0.12992594847733235 No Hit ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC 4301 0.12837388109372994 No Hit CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA 4015 0.1198375104839167 No Hit AATTCGTGGAGAAAGAAATGGCTCC 3906 0.11658413846828858 No Hit CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC 3766 0.11240549551243594 No Hit AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG 3697 0.11034602148419427 No Hit CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG 3545 0.1058092091321257 No Hit CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA 3507 0.10467500604410857 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 3425 0.10222751516996631 No Hit ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC 3389 0.10115300698131849 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.031399517068264104 0.0 0.0 0.0 0.0 2 0.06438094896981528 0.0 0.0 0.0 0.0 3 0.08079704629637921 0.0 0.0 0.0 0.0 4 0.10139178657879577 0.0 0.0 0.0 0.0 5 0.12515035652778647 0.0 0.0 0.0 0.0 6 0.16899626011455451 0.0 0.0 0.0 0.0 7 0.20630557222038162 0.0 0.0 0.0 0.0 8 0.26271725212439223 0.0 0.0 0.0 0.0 9 0.3065034608117909 0.0 0.0 0.0 0.0 10 0.35121494043941415 0.0 0.0 0.0 0.0 11 0.40010506302289 0.0 0.0 0.0 0.0 12 0.45323352346158785 0.0 0.0 0.0 0.0 13 0.5037354083280354 0.0 0.0 0.0 0.0 14 0.5555207335309235 0.0 0.0 0.0 0.0 15 0.6160215140417327 0.0 0.0 0.0 0.0 16 0.6673889749490355 0.0 0.0 0.0 0.0 17 0.73227733056349 0.0 0.0 0.0 0.0 18 0.7872264854329521 0.0 0.0 0.0 0.0 19 0.8539952303775404 0.0 0.0 0.0 0.0 20 0.9169733492121765 0.0 0.0 0.0 0.0 21 0.9767279434808693 0.0 0.0 0.0 0.0 22 1.0299160988189364 0.0 0.0 0.0 0.0 23 1.0771944591194405 0.0 0.0 0.0 0.0 24 1.1296364282153912 0.0 0.0 0.0 0.0 25 1.1805263299277393 0.0 0.0 0.0 0.0 26 1.2343412817091843 0.0 0.0 0.0 0.0 27 1.2938869438300844 0.0 0.0 0.0 0.0 28 1.3462692180266658 0.0 0.0 0.0 0.0 29 1.4047105245092333 0.0 0.0 0.0 0.0 30 1.4580180696460392 0.0 0.0 0.0 0.0 31 1.5103406489432512 0.0 0.0 0.0 0.0 32 1.561260398105284 0.0 0.0 0.0 0.0 33 1.6123890794151094 0.0 0.0 0.0 0.0 34 1.67232275838191 0.0 0.0 0.0 0.0 35 1.7210636437169626 0.0 0.0 0.0 0.0 36 1.7743711888537683 0.0 0.0 0.0 0.0 37 1.8241268874780994 0.0 0.0 0.0 0.0 38 1.8708381462345949 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAC 65 0.0 123.53686 44 AATCGCA 1245 0.0 109.645164 44 TAAGCCA 6605 0.0 100.9991 44 CTATCAT 7330 0.0 100.447426 44 AGACACG 980 0.0 88.24061 44 CCTTAAG 155 0.0 87.67132 44 CGGTAGG 50 6.9667294E-10 86.4758 44 AACGGTA 125 0.0 84.00506 44 CCCGTGA 150 0.0 78.240005 44 ACCACTC 1420 0.0 77.42803 44 CCCATAC 1560 0.0 75.23078 44 CCTAAGT 225 0.0 68.63159 44 AGAACGT 210 0.0 67.65113 44 TATAAGC 175 0.0 67.06287 44 TAGTCGA 130 0.0 65.56887 43 CCACTTT 290 0.0 63.898373 44 GAGGTAA 155 0.0 63.76096 44 GACATCA 325 0.0 62.71871 44 TAGACGG 350 0.0 61.82208 43 TACGGGA 160 0.0 61.76843 44 >>END_MODULE