FastQCFastQC Report
Tue 24 May 2016
ERR841246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4793259
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1398012.9166168571320683No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT984762.0544685776420595No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA890151.8570872135221568No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA586531.2236559718554745No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC278190.5803775677467043No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC180020.3755691065306507No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA107250.22375173133769738No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA87970.18352857627764324No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA75960.15847255489427967No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG74410.15523884688893297No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG70870.147853475057367No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC70520.14712328292712745No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG64770.13512726935890593No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC60600.12642755169290873No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC58350.12173345942708291No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG57600.12016876200514097No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC54650.11401428547883602No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAA54300.11328409334859643No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA53980.11261648911523453No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT53610.11184457172040985No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC52610.10975830849115392No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG51340.10710875418999892No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC50750.10587785888473793No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG49700.1036872824940192No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA48080.1003075360626246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAT251.2329292E-7118.5805644
TCGGGTA850.0118.5805544
ACGTTCC1450.0110.4025844
CTATCAT120300.0104.8299544
GTGTAAT850.0104.629944
TAAGCCA76000.0100.7934844
AATGCGG1000.094.8644544
TAGCGGG1500.083.0063944
AATCGCA14400.080.28892544
ATTAGTC13050.074.5103944
CTCCGTA1100.070.07033544
ACCACTC14050.067.5191944
TCTAGTG2050.066.52080544
ACTTCTA22250.065.8188744
CTTGAGA7300.064.9756544
GACATAT1100.064.68030544
CCCATAC31950.064.5790944
AGCGTAC1150.063.87438243
TGGTTAT2350.060.55177344
CGGTATG950.060.5125743
Produced by FastQC (version 0.11.5)