##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841246.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4793259
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.875391252590354	39.0	37.0	39.0	32.0	39.0
2	37.35665608722583	39.0	37.0	39.0	34.0	39.0
3	37.45825835824853	39.0	37.0	39.0	34.0	39.0
4	37.93134191163048	39.0	37.0	40.0	34.0	41.0
5	38.418974021641645	40.0	38.0	41.0	34.0	41.0
6	38.32631222306159	40.0	38.0	41.0	34.0	41.0
7	38.28584414069843	40.0	38.0	41.0	34.0	41.0
8	38.14128216313786	40.0	37.0	41.0	33.0	41.0
9	38.13724461791028	40.0	37.0	41.0	33.0	41.0
10	38.16170188174684	40.0	37.0	41.0	34.0	41.0
11	37.82326554855475	39.0	37.0	41.0	33.0	41.0
12	38.15339459019427	40.0	37.0	41.0	34.0	41.0
13	38.256586593797664	40.0	37.0	41.0	34.0	41.0
14	38.207365802682475	40.0	37.0	41.0	34.0	41.0
15	38.18411898877152	40.0	37.0	41.0	34.0	41.0
16	38.21807584359618	40.0	37.0	41.0	34.0	41.0
17	38.179235672430806	40.0	37.0	41.0	34.0	41.0
18	38.25240530503359	40.0	37.0	41.0	34.0	41.0
19	38.224391796896434	40.0	37.0	41.0	34.0	41.0
20	38.111425858690296	40.0	37.0	41.0	33.0	41.0
21	38.091216852667465	40.0	37.0	41.0	33.0	41.0
22	38.00385416268973	40.0	37.0	41.0	33.0	41.0
23	37.90187845054899	40.0	36.0	41.0	33.0	41.0
24	37.68395782493706	39.0	36.0	41.0	32.0	41.0
25	37.63465612602365	39.0	36.0	41.0	32.0	41.0
26	37.653176524188154	39.0	36.0	41.0	33.0	41.0
27	37.588489731461166	39.0	36.0	41.0	32.0	41.0
28	37.54803241051428	39.0	35.0	41.0	32.0	41.0
29	37.49562220644951	39.0	35.0	41.0	32.0	41.0
30	37.4458364735766	39.0	35.0	41.0	32.0	41.0
31	37.293621210931924	39.0	35.0	41.0	32.0	41.0
32	37.26158599267947	39.0	35.0	41.0	32.0	41.0
33	37.17455644241377	39.0	35.0	41.0	32.0	41.0
34	37.103650990447555	39.0	35.0	41.0	31.0	41.0
35	37.07856268120444	39.0	35.0	41.0	32.0	41.0
36	37.02524811180315	39.0	35.0	41.0	31.0	41.0
37	36.97254049103275	38.0	35.0	40.0	31.0	41.0
38	36.787182015914404	38.0	35.0	40.0	31.0	41.0
39	36.70430010856569	38.0	35.0	40.0	31.0	41.0
40	36.69899646199317	38.0	35.0	40.0	31.0	41.0
41	36.579943601183075	38.0	35.0	40.0	31.0	41.0
42	36.380896070778064	38.0	34.0	40.0	30.0	41.0
43	36.353883691468106	38.0	34.0	40.0	30.0	41.0
44	36.344266925085385	37.0	34.0	40.0	31.0	41.0
45	36.1636799205236	37.0	34.0	40.0	30.0	41.0
46	35.95334518147129	37.0	34.0	40.0	30.0	41.0
47	35.89670567927556	37.0	34.0	40.0	30.0	41.0
48	35.90550750415574	37.0	34.0	40.0	30.0	41.0
49	36.03657346608835	37.0	34.0	40.0	30.0	41.0
50	36.14308001352163	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	264.0
15	2975.0
16	4272.0
17	151.0
18	0.0
19	4.0
20	5.0
21	27.0
22	77.0
23	184.0
24	478.0
25	1310.0
26	2935.0
27	6568.0
28	14405.0
29	28799.0
30	54221.0
31	95152.0
32	156251.0
33	242061.0
34	359446.0
35	387258.0
36	506215.0
37	579423.0
38	662845.0
39	846104.0
40	841824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	39.54795682853774	22.196380375022507	38.25566279643975
2	25.92616005102165	23.037311357470983	28.823729324870616	22.212799266636747
3	27.948541900197753	20.877611662545252	25.22897260506891	25.94487383218808
4	24.853278322744504	22.420361595315423	27.200637395141804	25.525722686798275
5	22.335638445575338	23.84957708314948	26.30383628341385	27.510948187861327
6	25.093845335710004	24.355704542566965	21.952204126670395	28.598245995052636
7	27.751014497651806	21.39882280511026	20.904858260319337	29.945304436918597
8	23.518048993388422	22.36305194440776	28.418326654161607	25.70057240804221
9	26.604133012632953	25.622316674312824	25.541536562076033	22.232013750978197
10	35.193257864847276	19.35084250611119	22.047546356247388	23.408353272794148
11	25.508218938304815	21.12963226063937	24.70544571031943	28.656703090736386
12	26.974236109502954	22.655441735987978	24.502556611274294	25.867765543234782
13	28.645416406666115	21.246921979388137	22.368538816700703	27.73912279724505
14	21.22372273227881	23.160755552746053	21.070674461780598	34.54484725319454
15	24.54532500747404	28.41578141302191	24.6424405607959	22.396453018708147
16	22.742981341087557	28.8798498057376	20.87661025619521	27.500558596979634
17	22.90483364241323	32.77511188108132	18.913895535375826	25.406158941129615
18	22.786354753623787	23.987040967325154	21.42961605037408	31.79698822867698
19	28.17143826361146	22.441370266034028	20.91395436799889	28.47323710235562
20	24.34863211021979	20.06759910115435	24.664179423644747	30.91958936498111
21	24.400684377789723	27.992395153276718	22.16973879358491	25.437181675348654
22	25.83263287045411	23.368985485658087	22.515036220659056	28.283345423228745
23	30.104528046575407	22.381974351897114	24.932827539676033	22.580670061851446
24	26.821229564269323	21.863287587839505	25.332785063356685	25.982697784534487
25	23.698266737621385	27.33864737101836	20.974538689237665	27.98854720212259
26	24.971943171186986	28.614747401739706	24.429077142571032	21.984232284502276
27	23.703451119879716	25.88023430149108	23.2759158995321	27.140398679097107
28	23.95877110455464	21.89573720262069	22.894097177856036	31.251394514968634
29	25.241433977669566	24.164307794743184	24.718012257259144	25.87624597032811
30	23.779361274520067	26.005117724230814	24.45113345336041	25.764387547888713
31	23.932854065018933	30.362146817751178	23.32799713643888	22.37700198079101
32	25.633497632155343	24.97857791013494	22.904480752036154	26.483443705673565
33	23.521160025732073	21.94724925363363	26.405493739147495	28.12609698148681
34	25.665977777872907	26.003835938276225	26.566564884154708	21.763621399696163
35	21.997864221068795	23.567986577440386	22.45689086287786	31.977258338612963
36	21.740451014081906	23.387963322035343	28.24551907575304	26.62606658812971
37	22.14995502471044	29.989689267721037	22.35780849274729	25.50254721482123
38	26.294937736681433	26.802645474793035	20.717443185973785	26.184973602551747
39	25.514535321690783	28.512960308299295	24.665776799378175	21.306727570631747
40	24.769107051089446	25.276508312857604	24.360003519272166	25.594381116780784
41	27.187749901474252	28.432761437595893	21.808148366385694	22.57134029454416
42	21.81676177525195	31.09171272904416	21.827836530817134	25.26368896488675
43	23.514666471763388	23.940091604541248	23.407006772888952	29.138235150806413
44	24.50738931405132	24.00474718929048	22.530943698226764	28.95691979843143
45	22.810731899900706	23.347461145615746	29.18013109827498	24.66167585620857
46	21.62333114334531	26.420136586626136	30.042800641454555	21.913731628573995
47	24.78289582429189	30.964185708166912	23.514089949476237	20.73882851806496
48	26.246628448076613	27.027274995764415	20.328069661689156	26.398026894469815
49	21.488137592934613	28.616045968952907	20.363144787028656	29.532671651083824
50	18.415743945057432	30.653063160121892	27.002891586189453	23.928301308631227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10050.0
1	7614.0
2	4214.0
3	3839.0
4	4506.0
5	4161.0
6	3662.5
7	3296.5
8	3145.5
9	3865.0
10	4394.5
11	4678.5
12	5310.5
13	7471.5
14	10198.5
15	11252.0
16	11453.5
17	13954.5
18	17694.5
19	20782.5
20	22427.5
21	25134.5
22	28818.5
23	33055.5
24	36384.0
25	42475.0
26	51612.5
27	56518.0
28	61818.0
29	70775.0
30	77620.0
31	95526.0
32	115109.5
33	111276.5
34	115630.0
35	131064.5
36	136029.5
37	145753.0
38	154178.5
39	159052.0
40	170680.0
41	177937.5
42	180224.0
43	227506.0
44	278020.5
45	258155.5
46	235294.5
47	221039.5
48	228535.0
49	307747.0
50	427324.5
51	454449.0
52	367105.5
53	301017.0
54	274159.5
55	263832.0
56	271435.5
57	273272.0
58	270858.5
59	276865.0
60	275370.0
61	260670.0
62	246352.0
63	238325.5
64	226691.5
65	210125.0
66	236092.5
67	262360.0
68	205840.5
69	151011.5
70	133504.0
71	114921.0
72	100496.0
73	90508.5
74	78827.5
75	65387.5
76	59274.0
77	50453.0
78	40552.0
79	33338.0
80	24406.5
81	16486.0
82	13056.0
83	11821.5
84	10041.5
85	7122.0
86	4375.5
87	3021.0
88	1969.5
89	1560.5
90	1261.0
91	796.5
92	433.0
93	277.0
94	156.5
95	41.5
96	45.5
97	48.0
98	429.5
99	808.0
100	805.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	35295.0
25	34515.0
26	31204.0
27	35608.0
28	36844.0
29	34161.0
30	37449.0
31	36485.0
32	36349.0
33	36800.0
34	36408.0
35	36468.0
36	33294.0
37	39161.0
38	38651.0
39	37786.0
40	35133.0
41	37087.0
42	39961.0
43	39879.0
44	42395.0
45	58834.0
46	85515.0
47	183091.0
48	636822.0
49	1413292.0
50	1644772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.077544799466429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.36849892910728	6.206181119012439
2	13.283891841888163	2.4117024661201283
3	5.486855645258648	1.4942153378412195
4	2.8499651722498647	1.0348274611206882
5	1.715328467805219	0.7785485506150993
6	1.1209557517581732	0.6105315632882633
7	0.8230547363456501	0.5229921369113468
8	0.6301050151408827	0.45758452026478685
9	0.49083191660135955	0.4009993840761131
>10	3.353380179978497	6.0837586889221855
>50	0.5064166454358957	3.2601868240304097
>100	0.7275902282279488	16.322899008826713
>500	0.5380036053371651	35.65161754328078
>1k	0.09984281924985784	13.47715487863646
>5k	0.0036723795586154607	2.0674479962096464
>10k+	0.0016066660568942643	9.219352520843731
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	139801	2.9166168571320683	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	98476	2.0544685776420595	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	89015	1.8570872135221568	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	58653	1.2236559718554745	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	27819	0.5803775677467043	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	18002	0.3755691065306507	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10725	0.22375173133769738	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	8797	0.18352857627764324	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7596	0.15847255489427967	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	7441	0.15523884688893297	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	7087	0.147853475057367	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	7052	0.14712328292712745	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	6477	0.13512726935890593	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC	6060	0.12642755169290873	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	5835	0.12173345942708291	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	5760	0.12016876200514097	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	5465	0.11401428547883602	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAA	5430	0.11328409334859643	No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA	5398	0.11261648911523453	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	5361	0.11184457172040985	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	5261	0.10975830849115392	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG	5134	0.10710875418999892	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	5075	0.10587785888473793	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	4970	0.1036872824940192	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	4808	0.1003075360626246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03744842496514376	0.0	0.0	0.0	0.0
2	0.07291489986249439	0.0	0.0	0.0	0.0
3	0.09498756482802202	0.0	0.0	0.0	0.0
4	0.11983495988846002	0.0	0.0	0.0	0.0
5	0.15079510621061787	0.0	0.0	0.0	0.0
6	0.20209631901802094	0.0	0.0	0.0	0.0
7	0.25704849247662187	0.0	0.0	0.0	0.0
8	0.33476179776640486	0.0	0.0	0.0	0.0
9	0.3932188934501557	0.0	0.0	0.0	0.0
10	0.45236445599956104	0.0	0.0	0.0	0.0
11	0.5119689964594026	0.0	0.0	0.0	0.0
12	0.5819631278009387	0.0	0.0	0.0	0.0
13	0.643528755696281	0.0	0.0	0.0	0.0
14	0.7108941953689546	0.0	0.0	0.0	0.0
15	0.7857284574023644	0.0	0.0	0.0	0.0
16	0.8565779566678955	0.0	0.0	0.0	0.0
17	0.9438046222830855	0.0	0.0	0.0	0.0
18	1.019744603828001	0.0	0.0	0.0	0.0
19	1.1089323568786915	0.0	0.0	0.0	0.0
20	1.1986208131044036	0.0	0.0	0.0	0.0
21	1.2817375401579594	0.0	0.0	0.0	0.0
22	1.3489778040368776	0.0	0.0	0.0	0.0
23	1.414653370493854	0.0	0.0	0.0	0.0
24	1.4829159033551076	0.0	0.0	0.0	0.0
25	1.5464426186859503	0.0	0.0	0.0	0.0
26	1.6170626289962633	0.0	0.0	0.0	0.0
27	1.679170685331212	0.0	0.0	0.0	0.0
28	1.7425722248682993	0.0	0.0	0.0	0.0
29	1.8144439931161658	0.0	0.0	0.0	0.0
30	1.8803281858960679	0.0	0.0	0.0	0.0
31	1.94719292239372	0.0	0.0	0.0	0.0
32	2.0119088077652387	0.0	0.0	0.0	0.0
33	2.0761448525940285	0.0	0.0	0.0	0.0
34	2.152397773623332	0.0	0.0	0.0	0.0
35	2.216446054761489	0.0	0.0	0.0	0.0
36	2.278303759508927	0.0	0.0	0.0	0.0
37	2.3390974700094445	0.0	0.0	0.0	0.0
38	2.3942791324232635	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAT	25	1.2329292E-7	118.58056	44
TCGGGTA	85	0.0	118.58055	44
ACGTTCC	145	0.0	110.40258	44
CTATCAT	12030	0.0	104.82995	44
GTGTAAT	85	0.0	104.6299	44
TAAGCCA	7600	0.0	100.79348	44
AATGCGG	100	0.0	94.86445	44
TAGCGGG	150	0.0	83.00639	44
AATCGCA	1440	0.0	80.288925	44
ATTAGTC	1305	0.0	74.51039	44
CTCCGTA	110	0.0	70.070335	44
ACCACTC	1405	0.0	67.51919	44
TCTAGTG	205	0.0	66.520805	44
ACTTCTA	2225	0.0	65.81887	44
CTTGAGA	730	0.0	64.97565	44
GACATAT	110	0.0	64.680305	44
CCCATAC	3195	0.0	64.57909	44
AGCGTAC	115	0.0	63.874382	43
TGGTTAT	235	0.0	60.551773	44
CGGTATG	95	0.0	60.51257	43
>>END_MODULE