##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841245.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2067525 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.903048330733604 38.0 35.0 39.0 30.0 39.0 2 36.87564358351169 39.0 37.0 39.0 32.0 39.0 3 37.10197893616764 39.0 37.0 39.0 33.0 39.0 4 37.27864475641165 39.0 36.0 39.0 33.0 40.0 5 37.77921814730173 39.0 36.0 40.0 33.0 41.0 6 37.694453996928694 39.0 36.0 40.0 33.0 41.0 7 36.81416524588578 38.0 35.0 40.0 31.0 41.0 8 37.16055331858139 38.0 35.0 40.0 33.0 41.0 9 37.18802045924475 38.0 35.0 40.0 33.0 41.0 10 35.5728453102139 37.0 33.0 40.0 29.0 41.0 11 35.91453936469934 37.0 34.0 40.0 30.0 41.0 12 36.688024570440504 38.0 35.0 40.0 31.0 41.0 13 37.06867825056529 38.0 35.0 40.0 32.0 41.0 14 37.040726472473125 38.0 35.0 40.0 32.0 41.0 15 37.02339995888804 38.0 35.0 40.0 32.0 41.0 16 37.089565543342886 38.0 35.0 40.0 32.0 41.0 17 36.789566752518105 38.0 35.0 40.0 31.0 41.0 18 36.83091764307566 38.0 35.0 40.0 32.0 41.0 19 36.70452594285438 38.0 35.0 40.0 32.0 41.0 20 36.548174266332936 38.0 35.0 40.0 31.0 41.0 21 36.268330491771565 37.0 34.0 40.0 31.0 41.0 22 36.19275945877317 37.0 34.0 40.0 31.0 41.0 23 35.24584515302112 35.0 33.0 39.0 28.0 41.0 24 35.24653438289742 36.0 34.0 39.0 29.0 41.0 25 35.33713470641466 36.0 34.0 39.0 30.0 41.0 26 35.49627693175832 36.0 34.0 39.0 30.0 41.0 27 35.32662255791871 35.0 34.0 39.0 30.0 41.0 28 34.89846609084097 35.0 33.0 39.0 29.0 41.0 29 34.955640989156436 35.0 34.0 38.0 29.0 40.0 30 34.93569920275331 35.0 34.0 38.0 30.0 40.0 31 34.76569819066427 35.0 33.0 38.0 29.0 40.0 32 34.814233227754386 35.0 34.0 38.0 30.0 40.0 33 34.65991099810734 35.0 33.0 37.0 30.0 40.0 34 34.58684343427056 35.0 33.0 37.0 29.0 40.0 35 34.5332981129699 35.0 33.0 37.0 30.0 39.0 36 34.298290778670165 35.0 33.0 37.0 29.0 39.0 37 34.2451189364783 35.0 33.0 36.0 29.0 39.0 38 33.95486450509215 35.0 33.0 36.0 29.0 39.0 39 33.90892356139983 35.0 33.0 36.0 29.0 39.0 40 33.6980881529379 35.0 33.0 35.0 29.0 38.0 41 33.54627403733199 35.0 33.0 35.0 28.0 37.0 42 33.410767968695886 35.0 32.0 35.0 28.0 37.0 43 33.368411691927 35.0 33.0 35.0 27.0 37.0 44 33.39995345973286 35.0 33.0 35.0 29.0 37.0 45 33.304193779283786 35.0 33.0 35.0 29.0 37.0 46 33.084254303260025 34.0 32.0 35.0 27.0 36.0 47 33.1037340661274 34.0 32.0 35.0 28.0 36.0 48 33.184844643135165 34.0 32.0 35.0 29.0 36.0 49 33.08961441491023 34.0 32.0 35.0 27.0 36.0 50 32.9507018020777 34.0 32.0 35.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 211.0 14 608.0 15 350.0 16 132.0 17 1.0 18 0.0 19 0.0 20 1.0 21 24.0 22 80.0 23 246.0 24 753.0 25 1827.0 26 4258.0 27 9366.0 28 19063.0 29 35001.0 30 58331.0 31 90772.0 32 136692.0 33 200921.0 34 273698.0 35 293721.0 36 288712.0 37 304563.0 38 218361.0 39 103212.0 40 26621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 52.80942189332656 15.944329572798393 31.246248533875043 2 20.735662204810097 36.687053360902524 23.49707984183988 19.08020459244749 3 43.07473912044594 17.114569352244835 19.097810183673715 20.712881343635505 4 21.712143746750343 19.281895019407262 21.171739156721202 37.8342220771212 5 19.709362643740704 39.208860836023746 19.272705287723245 21.809071232512302 6 36.18684175523875 22.128825528107278 16.97348278739072 24.710849929263247 7 24.332426451917147 18.991354397165694 33.455363296695324 23.220855854221835 8 38.79358169792385 20.550174725819517 19.662591746169937 20.993651830086698 9 39.178341253431036 22.50386331483295 20.336827849723704 17.98096758201231 10 31.126201617876447 17.95644550851864 32.317819615240445 18.59953325836447 11 20.340745577441627 31.84541903967304 23.472654502363937 24.341180880521396 12 24.025634514697526 18.69341362256805 35.115125572846765 22.16582628988767 13 26.179175584333926 19.062647368230127 18.820473754851815 35.937703292584125 14 17.507115996179007 34.82637452993313 17.60293104073711 30.063578433150752 15 20.481928876313464 39.158123843726194 22.144689907014424 18.215257372945914 16 18.892395497031476 26.461542182077608 16.339826604273224 38.30623571661769 17 32.23288714767657 31.9022019080785 16.249331930690076 19.615579013554854 18 19.944329572798395 21.945321096480093 17.402788358060967 40.70756097266055 19 25.374590391893687 32.586498349475825 17.756351192851355 24.282560065779133 20 33.6559896494601 17.96955296792058 21.951512073614587 26.42294530900473 21 21.77797124581323 37.16970774234895 17.90667585639835 23.145645155439475 22 36.05692796943205 19.819059019842566 21.487769192633703 22.636243818091682 23 28.569908465435727 20.141328399898427 32.16817208981754 19.120591044848307 24 33.453428616340794 20.48033276502098 25.410236877425906 20.656001741212318 25 20.80273407080431 26.584442135603446 19.299369712693874 33.31345408089837 26 24.459342376960972 36.042226899673665 19.815781654569378 19.68264906879599 27 29.664053972803718 24.653481358384507 23.053977366341723 22.628487302470052 28 24.50616822662706 20.999474823713058 24.139842198018446 30.35451475164144 29 27.450191822894716 23.333986614010456 22.68283581087793 26.532985752216902 30 21.842238639809352 24.006123572689788 25.11255923522116 29.0390785522797 31 24.272114936882733 28.322977708085833 25.183117311767848 22.221790043263585 32 26.94070276618336 23.7087034507763 24.357997070212438 24.992596712827897 33 21.803579036581926 20.60967210249838 29.178274491797136 28.408474369122555 34 24.107307247221655 26.627169072980124 25.767302803020904 23.49822087677732 35 21.489203267563386 20.54571737291076 21.795165596935597 36.16991376259026 36 23.95356322597988 22.804198244679093 28.861988954966257 24.38024957437477 37 21.166894505201387 29.11181076858591 20.93117688526464 28.79011784094806 38 26.44422936838819 25.993872938974206 20.86328641694452 26.698611275693086 39 28.516914247848675 27.210706936224664 24.793013620764597 19.47936519516206 40 27.272222647104538 24.096871522989286 24.84994116065239 23.78096466925379 41 26.186775230544747 29.51448865398459 20.133875400896304 24.164860714574363 42 22.128608401859793 34.61371537430666 21.22705202885133 22.030624194982217 43 25.32071605320136 25.222381173316837 21.117189916486236 28.33971285699557 44 25.36928263930378 24.173616113390917 20.391238925442753 30.065862321862554 45 20.754759537151223 23.92183605791779 30.107194481796046 25.21620992313494 46 20.96507769048069 27.221170095180863 29.9235806821318 21.890171532206644 47 20.402408390268693 31.163785339371064 27.95425063847197 20.47955563188827 48 28.284068414848424 27.30781492107975 20.25555481417228 24.15256184989954 49 21.26909518213866 29.22613381130167 22.320538755596544 27.184232250963124 50 19.204154035146882 28.459373247215332 30.4672257225344 21.869246995103385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3931.0 1 2909.5 2 1730.0 3 1516.5 4 1464.5 5 1510.5 6 1514.0 7 1509.0 8 1857.0 9 2441.0 10 2691.5 11 2501.5 12 2079.5 13 1794.5 14 1810.0 15 1842.0 16 1945.0 17 2832.0 18 4647.5 19 7583.5 20 10331.0 21 12752.5 22 14089.0 23 15462.5 24 16782.0 25 17382.5 26 18904.0 27 22253.5 28 25820.5 29 25316.0 30 25344.0 31 31690.5 32 37271.0 33 37661.0 34 40894.5 35 50650.5 36 55401.0 37 52882.5 38 56539.0 39 67581.5 40 78495.5 41 85689.5 42 90367.0 43 92977.5 44 95564.5 45 103101.5 46 109845.0 47 107799.0 48 137469.0 49 205063.0 50 287839.0 51 317930.5 52 266961.0 53 217200.5 54 184179.0 55 158369.5 56 144380.0 57 122237.0 58 102062.0 59 97026.5 60 95335.0 61 93906.5 62 89910.5 63 84891.0 64 77056.5 65 70722.0 66 93749.5 67 118439.5 68 88181.0 69 57127.5 70 52133.5 71 44558.5 72 36691.5 73 30845.5 74 27550.0 75 24180.5 76 21156.5 77 16670.5 78 12202.5 79 9751.0 80 7474.5 81 5582.5 82 4297.5 83 3171.0 84 2401.0 85 2042.5 86 1765.0 87 1452.5 88 1114.0 89 895.0 90 529.0 91 90.0 92 6.5 93 6.0 94 4.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 38609.0 25 26401.0 26 29966.0 27 45577.0 28 64577.0 29 35357.0 30 27372.0 31 23428.0 32 25264.0 33 18379.0 34 17678.0 35 18589.0 36 16776.0 37 17091.0 38 17546.0 39 23551.0 40 25253.0 41 22388.0 42 21883.0 43 21982.0 44 22411.0 45 36353.0 46 38768.0 47 83496.0 48 273166.0 49 494446.0 50 581218.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.603101750442736 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15984133659951 5.949891520632412 2 12.501822399064883 2.1510890033023857 3 5.050217518665044 1.3034260552483146 4 2.661761300510934 0.915976132147454 5 1.6680887541144278 0.7175368640207839 6 1.1123214795502219 0.5741648920664141 7 0.815425850542305 0.49106340935097326 8 0.6102021309974905 0.41997048170467194 9 0.5031609768225261 0.3895870572410717 >10 3.773067422293407 6.75345091949946 >50 0.6032888531101286 3.6824974012356426 >100 0.9223466450715284 20.382813034932948 >500 0.539814327596674 31.678988102957774 >1k 0.06853001869593572 9.765465832254922 >5k 0.003932050253045492 2.408783010479899 >10k+ 0.006178936111928631 12.415296282924885 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 49156 2.377528687682132 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA 42725 2.066480453682543 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 42273 2.0446185656763523 No Hit AAGCAGTGGTATCAACGCAGAGTGCAG 28099 1.3590645820485847 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG 15376 0.7436911282813993 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGT 14577 0.7050458881996591 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGG 14232 0.6883592701418363 No Hit AAGCAGTGGTATCAACGCAGAGTG 14206 0.6871017279113916 No Hit AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 13014 0.6294482533463924 Illumina Single End Adapter 2 (100% over 34bp) AAGCAGTGGTATCAACGCAGAGTGCAGTG 11909 0.5760027085524964 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGA 11343 0.5486269815358944 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCA 9611 0.4648553221847378 No Hit AAGCAGTGGTATCAACGCAGAGTGC 7557 0.3655094859796133 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGG 7353 0.3556426161715094 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 7185 0.34751695868248267 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTG 6529 0.3157882008681878 No Hit AAGCAGTGGTATCAACGCAGAGTGCA 6360 0.3076141763702978 No Hit AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 5250 0.25392679653208544 Illumina Single End Adapter 2 (100% over 32bp) AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAGCACTGCA 4504 0.2178450079200977 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGC 4389 0.21228280190082344 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC 4222 0.20420551142065996 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGG 4081 0.19738576317094111 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 3970 0.19201702518711988 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 3799 0.18374626667150337 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 3596 0.17392776387226272 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC 3234 0.15641890666376465 Illumina Single End Adapter 2 (100% over 25bp) AAGCAGTGGTATCAACGCAGATCGGAAG 3159 0.15279138099902057 No Hit AAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGC 3022 0.14616510078475473 Illumina Single End Adapter 2 (96% over 31bp) AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA 2836 0.1371688371361894 No Hit AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGA 2694 0.1303007218776073 No Hit TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGGA 2655 0.12841440853194036 No Hit AAGCAGTGGTATCAACGCAGAGTAGATCGGAAGAGCTCGTATGCCGTCTT 2560 0.1238195426899312 Illumina Single End Adapter 2 (100% over 27bp) AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG 2541 0.12290056952152936 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAAGCACTGCA 2376 0.11492001305909239 No Hit CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC 2372 0.11472654502363937 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGGG 2328 0.11259839663365619 No Hit CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC 2295 0.1110022853411688 No Hit AAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA 2221 0.10742312668528796 Illumina Single End Adapter 2 (97% over 34bp) AAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA 2149 0.10394070204713364 Illumina Single End Adapter 2 (100% over 34bp) AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT 2072 0.10021644236466307 Illumina Single End Adapter 2 (95% over 24bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11709652845793884 0.0 0.0 0.0 0.0 2 0.3001172899964934 0.0 0.0 0.0 0.0 3 0.31225740922117023 0.0 0.0 0.0 0.0 4 0.33808539195414805 0.0 0.0 0.0 0.0 5 0.6693994026674406 0.0 0.0 0.0 0.0 6 0.7211037351422594 0.0 0.0 0.0 0.0 7 0.9416089285498361 0.0 0.0 0.0 0.0 8 1.2120288751042914 0.0 0.0 0.0 0.0 9 1.2711817269440515 0.0 0.0 0.0 0.0 10 1.365594128245124 0.0 0.0 0.0 0.0 11 1.6119273043856785 0.0 0.0 0.0 0.0 12 1.6839941475919276 0.0 0.0 0.0 0.0 13 1.765299089491058 0.0 0.0 0.0 0.0 14 1.9197833158002926 0.0 0.0 0.0 0.0 15 2.1413042163939977 0.0 0.0 0.0 0.0 16 2.2055356041643996 0.0 0.0 0.0 0.0 17 3.2219199284168267 0.0 0.0 0.0 0.0 18 3.303950375448906 0.0 0.0 0.0 0.0 19 3.7430260819095293 0.0 0.0 0.0 0.0 20 3.8026142368290587 0.0 0.0 0.0 0.0 21 3.9723340709302186 0.0 0.0 0.0 0.0 22 4.013155826410805 0.0 0.0 0.0 0.0 23 4.100409910400116 0.0 0.0 0.0 0.0 24 4.316465338992273 0.0 0.0 0.0 0.0 25 4.369378846688674 0.0 0.0 0.0 0.0 26 4.640911234446984 0.0 0.0 0.0 0.0 27 4.685602350636631 0.0 0.0 0.0 0.0 28 4.742240118015502 0.0 0.0 0.0 0.0 29 4.892854983615676 0.0 0.0 0.0 0.0 30 4.949154181932504 0.0 0.0 0.0 0.0 31 4.989008597235825 0.0 0.0 0.0 0.0 32 5.028040773388471 0.0 0.0 0.0 0.0 33 5.068814161860195 0.0 0.0 0.0 0.0 34 5.114327517200517 0.0 0.0 0.0 0.0 35 5.152102151122719 0.0 0.0 0.0 0.0 36 5.195293890037606 0.0 0.0 0.0 0.0 37 5.2357770764561495 0.0 0.0 0.0 0.0 38 5.267263999226127 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGC 75 0.0 136.55977 44 GTACACC 45 0.0 136.55977 44 CATGACG 135 0.0 136.55977 44 GTTAATT 75 0.0 136.55977 44 AATAAGG 50 0.0 136.55977 44 CGAAGGT 25 5.306356E-8 136.55977 44 GATCCTA 95 0.0 129.37242 44 CTATCAT 5670 0.0 127.76888 44 TTACTTG 75 0.0 127.45579 44 ACGTGTG 65 0.0 126.05516 44 GACCCTT 90 0.0 121.38647 44 CGTATTG 110 0.0 117.93798 44 AATACGA 35 2.8849172E-9 117.05123 44 GGATAGA 100 0.0 116.075806 44 TGTGGAC 190 0.0 107.81035 44 CTTAACC 365 0.0 97.27546 44 AATAGGC 95 0.0 93.43563 44 ACCACTC 315 0.0 91.03985 44 ACGAACA 190 0.0 89.84196 44 TATAGAC 70 0.0 87.78842 44 >>END_MODULE