FastQCFastQC Report
Tue 24 May 2016
ERR841244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4272074
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC949882.223463357610379No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT740751.7339353204087755No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA647191.5149316233754377No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG467821.0950653008351448No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA213490.4997338529248323No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC173820.40687497454398025No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA167050.39102787077190143No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC147920.34624868389452057No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC129950.3041848057875402No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC73530.1721178050754739No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA71500.16736601472727297No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG62980.14742253996536575No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG62470.14622874041975864No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA59510.13930002148839182No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG55600.1301475583054039No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC50690.11865431169965689No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC49440.11572833242120806No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG48740.11408978402527672No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC48710.11401956052259393No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC46230.10821441763415147No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAA44690.10460961116310252No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTG43460.10173044755310887No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC43090.100864357686688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG152.9871543E-4116.03599544
ACGAACA2350.0111.0982944
CTATCAT89550.0101.4586244
TAACGAT800.094.2792544
TAAGCCA29750.093.2188444
ACTTCTA22950.087.216644
CACAACG600.087.02744
CTCTATT1150.080.72069544
TCTATTC2200.076.4782744
CGACGGG3850.073.8410944
TTGCGTG950.073.285944
AATCGCA10600.070.0594744
CTTAACC5750.069.621644
GTAGAAC1950.068.4314944
ACCACTC15100.067.62363444
ACGAGTT1350.064.4644444
TACGGAC1450.064.0198644
CTAGTAC1750.062.9909744
GTTGATT1300.062.48092344
CTTAGCC25400.059.61691744
Produced by FastQC (version 0.11.5)