##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841244.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4272074
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.75838714404291	39.0	37.0	39.0	32.0	39.0
2	37.30836848799904	39.0	37.0	39.0	34.0	39.0
3	37.435453131195764	39.0	37.0	39.0	33.0	39.0
4	37.889283518965264	39.0	37.0	40.0	34.0	41.0
5	38.42553663630358	40.0	38.0	41.0	34.0	41.0
6	38.400982988590556	40.0	38.0	41.0	34.0	41.0
7	38.28030483554358	40.0	37.0	41.0	34.0	41.0
8	38.1972793542434	40.0	37.0	41.0	33.0	41.0
9	38.060222739587374	40.0	37.0	41.0	33.0	41.0
10	37.9491544388042	40.0	37.0	41.0	33.0	41.0
11	37.57685002647426	39.0	36.0	41.0	32.0	41.0
12	37.865330750356854	39.0	36.0	41.0	33.0	41.0
13	37.975998543096395	39.0	37.0	41.0	33.0	41.0
14	37.94128378862351	40.0	36.0	41.0	33.0	41.0
15	37.85668904611671	39.0	36.0	41.0	33.0	41.0
16	37.93332957247463	40.0	36.0	41.0	33.0	41.0
17	37.826699397061006	39.0	36.0	41.0	33.0	41.0
18	37.8246252288701	39.0	36.0	41.0	33.0	41.0
19	37.76403124103187	39.0	36.0	41.0	33.0	41.0
20	37.626110409136174	39.0	35.0	41.0	33.0	41.0
21	37.530729102538956	39.0	35.0	41.0	33.0	41.0
22	37.438589780982255	39.0	35.0	41.0	32.0	41.0
23	37.298620997669985	39.0	35.0	41.0	32.0	41.0
24	37.00499616813754	39.0	35.0	41.0	31.0	41.0
25	36.93868527486123	38.0	35.0	40.0	31.0	41.0
26	36.906434245431434	38.0	35.0	40.0	31.0	41.0
27	36.797630938294546	38.0	35.0	40.0	31.0	41.0
28	36.748146704216076	38.0	35.0	40.0	31.0	41.0
29	36.64659099130455	38.0	35.0	40.0	31.0	41.0
30	36.535565570349874	38.0	35.0	40.0	31.0	41.0
31	36.3501482527962	37.0	34.0	40.0	31.0	41.0
32	36.276191913376294	37.0	34.0	40.0	31.0	41.0
33	36.18936823568202	37.0	34.0	40.0	31.0	41.0
34	36.118039667008404	37.0	34.0	40.0	31.0	41.0
35	36.09616951747314	36.0	34.0	40.0	31.0	41.0
36	35.98474141451295	36.0	34.0	40.0	31.0	41.0
37	35.912448228105184	36.0	34.0	40.0	31.0	41.0
38	35.67207876460201	36.0	34.0	39.0	30.0	41.0
39	35.56188522284301	35.0	34.0	39.0	30.0	41.0
40	35.50424369459606	35.0	34.0	39.0	30.0	41.0
41	35.36907376216742	35.0	34.0	39.0	30.0	41.0
42	35.05609495062484	35.0	33.0	39.0	30.0	41.0
43	35.00768566515542	35.0	33.0	39.0	30.0	40.0
44	34.95236226176077	35.0	33.0	38.0	30.0	40.0
45	34.76270247052533	35.0	33.0	37.0	30.0	40.0
46	34.52559401115435	35.0	33.0	37.0	29.0	40.0
47	34.44995033439542	35.0	33.0	37.0	29.0	40.0
48	34.41262720905508	35.0	33.0	37.0	29.0	39.0
49	34.46810139415868	35.0	33.0	37.0	29.0	39.0
50	34.74373503168301	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	100.0
14	982.0
15	3165.0
16	490.0
17	6.0
18	0.0
19	1.0
20	2.0
21	18.0
22	62.0
23	219.0
24	604.0
25	1592.0
26	3938.0
27	9158.0
28	19449.0
29	38806.0
30	70398.0
31	118203.0
32	183075.0
33	275385.0
34	399725.0
35	443595.0
36	518213.0
37	586962.0
38	568811.0
39	662788.0
40	366327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.396818968959806	22.139504137802856	37.463676893237334
2	26.115699306706762	21.83576408086564	28.360814910977666	23.68772170144993
3	28.625861817936677	20.115733013988056	24.965812858110603	26.292592309964668
4	24.833043622371708	21.939835311841506	27.6125600820585	25.61456098372828
5	23.14142966624642	25.00286745969288	24.84741603258745	27.00828684147325
6	24.24304916066529	23.078298737334606	21.777782875483897	30.90086922651621
7	27.639245013077957	21.641713135118916	21.444853249264877	29.274188602538253
8	25.656484414829894	22.491909082099234	25.24771340571348	26.603893097357396
9	29.006356163306158	25.51030249007859	22.457757051961178	23.025584294654074
10	32.78077580116824	19.829408385716167	22.471403819315864	24.918411993799733
11	25.49717069507691	21.214613791802293	25.410678747605964	27.877536765514833
12	28.341105514558034	20.816399715922522	23.254536321234138	27.587958448285306
13	28.489113250379088	21.077514106731297	22.14451341432756	28.288859228562053
14	21.5326794432868	24.23883575050432	20.334174922999928	33.89430988320895
15	23.421574626282222	28.776023074506668	24.32141858965926	23.480983709551847
16	22.117383734457782	28.71755030460615	20.587400873674007	28.577665087262066
17	23.843992402753322	33.04462890858164	18.791107082882927	24.32027160578211
18	23.62651021494478	23.978915159241154	20.04387096290935	32.35070366290472
19	29.172973127338146	21.576335054121255	20.48478092842025	28.76591089012035
20	24.866025260798384	20.900152946788843	23.210529592886264	31.02329219952651
21	23.03525173019007	28.623895559861555	21.031283634131807	27.30956907581657
22	25.421937915869435	23.314390153354086	24.569143699289853	26.694528231486625
23	30.496615929405717	22.066822812526187	25.01082612333026	22.425735134737835
24	27.87472782540752	22.424705190031823	24.550019498725913	25.15054748583475
25	25.776158624548746	27.87782056518731	20.07144526740032	26.27457554286362
26	24.588217673941497	30.511906595922454	22.296594710470742	22.603281019665307
27	24.293557499927353	25.98294830817159	22.394532853241664	27.328961338659397
28	23.418626669927622	22.939508483728723	21.379383824857676	32.262481021485975
29	24.321908540388762	24.442553728170946	24.281481648048462	26.95405608339183
30	23.595431669652125	26.670178390475257	23.913310871118668	25.82107906875395
31	24.735019427472306	30.15463616184096	22.70055174071097	22.40979266997577
32	27.600460095549423	24.367713866969513	21.882183500387054	26.14964253709401
33	23.340185968255028	22.234647793304678	26.15158960364623	28.27357663479406
34	24.743761260329364	27.644795001346	25.407721616818556	22.203722121506072
35	22.83376997736594	22.78375251503872	23.077907735038288	31.304569772557052
36	22.304654258220776	24.623782612174242	26.921376462605817	26.150186666999165
37	22.68857857841338	29.155012996837954	21.893679761999252	26.262728662749407
38	25.63242586750622	26.87830416585849	21.42291261807529	26.066357348559997
39	27.095112020800798	27.79632028981604	23.09739560737563	22.01117208200753
40	25.748650259454237	24.99992600475453	23.874175793868083	25.377247941923148
41	26.561762814949898	27.95143599743268	21.514388765710656	23.972412421906768
42	21.80987129603889	30.13464304511836	22.39578583256508	25.65969982627767
43	25.518958075892296	23.806924847910153	22.855097811622738	27.819019264574806
44	26.704131332971713	23.670346873845375	21.40087642163023	28.224645371552683
45	23.354938522366428	22.851777002177755	28.549842591577274	25.243441883878546
46	22.22000871460199	26.823683709101147	29.301880578725903	21.654426997570955
47	23.181516731561093	30.856046382074403	23.678939130896133	22.283497755468375
48	26.545960624659475	27.36013559916059	20.21708453166274	25.8768192445172
49	22.846368174095236	25.962023774939187	21.03691101857802	30.154697032387563
50	21.471167892762935	29.46167406261177	25.042963137929625	24.024194906695673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9824.0
1	7629.0
2	4590.0
3	3740.5
4	3611.0
5	3322.0
6	3078.0
7	2966.0
8	3193.0
9	4424.0
10	5576.0
11	5420.0
12	5271.0
13	7913.0
14	11423.0
15	12466.0
16	13273.0
17	15589.0
18	18173.0
19	21893.5
20	24684.0
21	24128.0
22	23270.0
23	29557.0
24	35586.0
25	37551.0
26	41303.5
27	47435.0
28	57317.0
29	63838.0
30	68351.0
31	84070.0
32	96780.5
33	93152.0
34	104337.0
35	122583.0
36	117657.0
37	119133.0
38	130405.5
39	125731.0
40	121861.5
41	137469.0
42	156841.5
43	180192.0
44	196815.5
45	188685.0
46	189441.0
47	193027.0
48	204782.5
49	274740.5
50	375654.5
51	396129.0
52	329620.5
53	272538.0
54	256299.0
55	259006.0
56	263593.5
57	263798.0
58	251872.5
59	235141.5
60	231745.5
61	234268.0
62	226482.0
63	218466.5
64	211931.0
65	201646.5
66	240855.5
67	277083.0
68	216453.0
69	159811.5
70	140553.5
71	120619.0
72	104946.5
73	89708.5
74	75847.5
75	62253.5
76	53509.0
77	42961.5
78	33405.5
79	29112.0
80	24980.0
81	18687.0
82	13299.0
83	9864.0
84	7477.0
85	5478.5
86	3710.5
87	2422.5
88	1476.5
89	1173.0
90	883.5
91	492.5
92	287.0
93	194.0
94	131.0
95	54.5
96	27.0
97	22.0
98	177.5
99	332.5
100	332.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	36664.0
25	35679.0
26	35804.0
27	34257.0
28	38334.0
29	34449.0
30	37064.0
31	37172.0
32	33805.0
33	33552.0
34	32650.0
35	33008.0
36	32169.0
37	33229.0
38	33997.0
39	33786.0
40	34798.0
41	41365.0
42	39953.0
43	38583.0
44	41614.0
45	61579.0
46	65873.0
47	151372.0
48	515086.0
49	1236001.0
50	1490231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.533003066995636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22099887070044	6.503509914678932
2	13.327212241461375	2.540967103447062
3	5.390061595794618	1.5415042117201647
4	2.922065116094038	1.1142422285474172
5	1.758771486964862	0.8383186989690254
6	1.1246185940592335	0.6432595503820195
7	0.8251527617513191	0.5506328665960671
8	0.6109111702386392	0.46590544476374735
9	0.47401756430845027	0.4066929804926033
>10	3.4279582818050005	6.4965281816559735
>50	0.5450523289357596	3.658864787956283
>100	0.7661466667438788	18.371871516765363
>500	0.5261282339142892	35.70000633201811
>1k	0.07698241633228649	11.645686079276762
>5k	0.001716168516961801	1.0196636274116693
>10k+	0.002206502378950887	8.502346475318808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	94988	2.223463357610379	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	74075	1.7339353204087755	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	64719	1.5149316233754377	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	46782	1.0950653008351448	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	21349	0.4997338529248323	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	17382	0.40687497454398025	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	16705	0.39102787077190143	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	14792	0.34624868389452057	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	12995	0.3041848057875402	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC	7353	0.1721178050754739	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	7150	0.16736601472727297	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	6298	0.14742253996536575	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	6247	0.14622874041975864	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5951	0.13930002148839182	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	5560	0.1301475583054039	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	5069	0.11865431169965689	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	4944	0.11572833242120806	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	4874	0.11408978402527672	No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC	4871	0.11401956052259393	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	4623	0.10821441763415147	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAA	4469	0.10460961116310252	No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTG	4346	0.10173044755310887	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	4309	0.100864357686688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.015449170590209813	0.0	0.0	0.0	0.0
2	0.034315884977647856	0.0	0.0	0.0	0.0
3	0.05065455326850612	0.0	0.0	0.0	0.0
4	0.07052780452773055	0.0	0.0	0.0	0.0
5	0.10027916183099825	0.0	0.0	0.0	0.0
6	0.13995544084676437	0.0	0.0	0.0	0.0
7	0.18007646871285468	0.0	0.0	0.0	0.0
8	0.24807622714400546	0.0	0.0	0.0	0.0
9	0.29257452001065526	0.0	0.0	0.0	0.0
10	0.3408648820221747	0.0	0.0	0.0	0.0
11	0.39699686849993704	0.0	0.0	0.0	0.0
12	0.45448650936289964	0.0	0.0	0.0	0.0
13	0.5112036916963517	0.0	0.0	0.0	0.0
14	0.5833700446200136	0.0	0.0	0.0	0.0
15	0.6594220980254556	0.0	0.0	0.0	0.0
16	0.7257599002264474	0.0	0.0	0.0	0.0
17	0.8252900113621627	0.0	0.0	0.0	0.0
18	0.901435696104515	0.0	0.0	0.0	0.0
19	0.9993038510100715	0.0	0.0	0.0	0.0
20	1.084016803079722	0.0	0.0	0.0	0.0
21	1.1684488611386412	0.0	0.0	0.0	0.0
22	1.2318606840611843	0.0	0.0	0.0	0.0
23	1.3005860853533904	0.0	0.0	0.0	0.0
24	1.3717693092394936	0.0	0.0	0.0	0.0
25	1.439441357991458	0.0	0.0	0.0	0.0
26	1.525488556612081	0.0	0.0	0.0	0.0
27	1.5858807689192649	0.0	0.0	0.0	0.0
28	1.6463666125633591	0.0	0.0	0.0	0.0
29	1.7192586083480763	0.0	0.0	0.0	0.0
30	1.7837471916450884	0.0	0.0	0.0	0.0
31	1.8452395721609691	0.0	0.0	0.0	0.0
32	1.9004586531038554	0.0	0.0	0.0	0.0
33	1.9632384645022534	0.0	0.0	0.0	0.0
34	2.0394543727472887	0.0	0.0	0.0	0.0
35	2.101976697969183	0.0	0.0	0.0	0.0
36	2.16379678816425	0.0	0.0	0.0	0.0
37	2.222456820738592	0.0	0.0	0.0	0.0
38	2.2771843371626987	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	15	2.9871543E-4	116.035995	44
ACGAACA	235	0.0	111.09829	44
CTATCAT	8955	0.0	101.45862	44
TAACGAT	80	0.0	94.27925	44
TAAGCCA	2975	0.0	93.21884	44
ACTTCTA	2295	0.0	87.2166	44
CACAACG	60	0.0	87.027	44
CTCTATT	115	0.0	80.720695	44
TCTATTC	220	0.0	76.47827	44
CGACGGG	385	0.0	73.84109	44
TTGCGTG	95	0.0	73.2859	44
AATCGCA	1060	0.0	70.05947	44
CTTAACC	575	0.0	69.6216	44
GTAGAAC	195	0.0	68.43149	44
ACCACTC	1510	0.0	67.623634	44
ACGAGTT	135	0.0	64.46444	44
TACGGAC	145	0.0	64.01986	44
CTAGTAC	175	0.0	62.99097	44
GTTGATT	130	0.0	62.480923	44
CTTAGCC	2540	0.0	59.616917	44
>>END_MODULE