FastQCFastQC Report
Tue 24 May 2016
ERR841243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007659
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC744042.4738176768044515No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA559401.8599182952588709No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT445191.480187747347688No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA169830.5646584270357776No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG138090.4591278466076108No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC107800.358418291435299No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA96430.32061480373938667No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC71130.23649622513722468No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA70220.23347061618355006No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG58650.1950021594868301No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT52280.1738228968111079No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG42900.14263585067323123No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA40300.13399125366273237No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG39690.13196309821026916No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC38240.12714207295441404No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA37650.12518041440203162No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC34970.11626982979120971No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC34460.11457415883915031No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGTA33470.11128256228515267No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT31820.10579656802848993No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG31370.10430038777667283No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC31300.10406764862639017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGG800.0116.3622244
CTAACCA2200.0108.42844444
TACGCGC1200.096.9685244
TAGGACA850.095.8277244
CTATCAT56250.094.95158444
GATCGTA1450.092.28728544
TATCGTT900.090.5039544
CGACGGG4550.088.230744
TAAGCCA28800.080.2010544
ACGAACA3000.075.63544544
ACTTCTA15200.074.2574844
ATAGTGT1500.073.69607544
GTTAATG950.073.49193644
TAGCGGG1700.071.8707944
TGTGCAC1150.070.82918544
AACTACC3900.070.115744
ATCGCAA2650.068.0609344
TATACCG2000.066.9082844
GGTGCAA2100.066.4927144
TCGTCCC2450.066.492744
Produced by FastQC (version 0.11.5)