##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841243.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3007659
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.649027698951244	38.0	35.0	39.0	30.0	39.0
2	36.40231322766311	38.0	35.0	39.0	32.0	39.0
3	36.502089498842786	38.0	35.0	39.0	33.0	39.0
4	36.99547621588751	38.0	35.0	39.0	33.0	40.0
5	37.589142253161015	39.0	35.0	41.0	33.0	41.0
6	37.39755005471032	39.0	35.0	40.0	33.0	41.0
7	37.48272593402377	39.0	35.0	40.0	33.0	41.0
8	37.232684290340096	38.0	35.0	40.0	33.0	41.0
9	37.17123051516146	38.0	35.0	40.0	33.0	41.0
10	37.196689185841876	38.0	35.0	40.0	33.0	41.0
11	36.82827441541744	38.0	35.0	40.0	31.0	41.0
12	37.184714756559835	39.0	35.0	40.0	32.0	41.0
13	37.40798375081749	39.0	35.0	41.0	33.0	41.0
14	37.37546909406951	39.0	35.0	41.0	33.0	41.0
15	37.28072830064844	39.0	35.0	41.0	33.0	41.0
16	37.40253565979388	39.0	35.0	41.0	33.0	41.0
17	37.37417639433194	39.0	35.0	41.0	33.0	41.0
18	37.31837984292767	39.0	35.0	41.0	33.0	41.0
19	37.36368052362319	39.0	35.0	41.0	33.0	41.0
20	37.275227012104764	39.0	35.0	41.0	33.0	41.0
21	37.18073923938851	38.0	35.0	41.0	33.0	41.0
22	37.101864606326714	38.0	35.0	41.0	32.0	41.0
23	37.036399405650705	38.0	35.0	41.0	32.0	41.0
24	36.83561135088785	38.0	35.0	40.0	31.0	41.0
25	36.827123649021196	38.0	35.0	40.0	31.0	41.0
26	36.788651359982644	38.0	35.0	40.0	32.0	41.0
27	36.768268930587475	38.0	35.0	40.0	32.0	41.0
28	36.777600895522184	38.0	35.0	40.0	32.0	41.0
29	36.686884063665985	38.0	35.0	40.0	31.0	41.0
30	36.65859708048056	38.0	35.0	40.0	31.0	41.0
31	36.48385963071204	38.0	35.0	40.0	31.0	41.0
32	36.430126027521844	38.0	34.0	40.0	31.0	41.0
33	36.30973005857138	37.0	34.0	40.0	31.0	41.0
34	36.235286500345076	37.0	34.0	40.0	31.0	41.0
35	36.257760683386586	37.0	34.0	40.0	31.0	41.0
36	36.26735555804799	37.0	34.0	40.0	31.0	41.0
37	36.10598112435086	37.0	34.0	40.0	31.0	41.0
38	36.06507321709908	36.0	34.0	40.0	31.0	41.0
39	35.961034486536434	36.0	34.0	40.0	31.0	41.0
40	35.98106658095403	36.0	34.0	40.0	31.0	41.0
41	35.79716058589839	36.0	34.0	40.0	31.0	41.0
42	35.631711781565215	36.0	34.0	39.0	30.0	41.0
43	35.543280707028764	35.0	34.0	39.0	30.0	41.0
44	35.53826270394615	35.0	34.0	39.0	30.0	41.0
45	35.289381516355924	35.0	33.0	39.0	30.0	41.0
46	35.06021548157496	35.0	33.0	39.0	29.0	41.0
47	35.06375492097409	35.0	33.0	39.0	30.0	40.0
48	35.088571090846514	35.0	33.0	39.0	30.0	40.0
49	35.308859207478314	36.0	33.0	39.0	30.0	41.0
50	35.64542249589833	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	50.0
15	299.0
16	171.0
17	1.0
18	0.0
19	1.0
20	7.0
21	40.0
22	145.0
23	459.0
24	1188.0
25	2752.0
26	5670.0
27	11425.0
28	21493.0
29	38564.0
30	64490.0
31	103489.0
32	159180.0
33	245622.0
34	376838.0
35	221759.0
36	266879.0
37	323368.0
38	377008.0
39	448223.0
40	338536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	43.12257473337237	21.82680948870866	35.05061577791898
2	27.185694920867025	21.048031043412834	28.006565903913977	23.759708131806164
3	27.126911661195635	21.602382450936094	24.79430015171268	26.476405736155595
4	23.58901058929885	23.101954044657322	27.563962536976433	25.74507282906739
5	23.201765891678544	25.31816273054891	25.207744627964807	26.27232674980774
6	22.188053898397392	25.207046410513957	22.992034668823827	29.612865022264824
7	28.444979966146427	21.603047419936903	20.962349787658773	28.9896228262579
8	25.72745115054599	21.90836793665771	27.22645752061653	25.137723392179765
9	26.59836105090371	25.688151482598258	25.21067049156836	22.502816974929672
10	34.05356125810805	19.91622055558825	22.407427171763818	23.622791014539878
11	25.10417570608902	21.232692934937106	26.057874247047287	27.605257111926583
12	28.119710379401386	21.948099834455967	23.058697811154786	26.873491974987857
13	28.452926345706082	21.765865079784643	22.464348518232953	27.316860056276326
14	22.546206202232366	22.6487444221569	21.49096689485078	33.31408248075996
15	23.527168472223746	27.86878432694664	24.90019646509129	23.703850735738328
16	22.648611428356737	29.25268456297739	21.82518031465668	26.273523694009192
17	23.160637558978596	32.445134238954616	20.103708565365956	24.290519636700836
18	23.892535689717484	23.17749452314907	21.669777059167945	31.260192727965503
19	28.620332291659395	21.522985152239666	20.64033854901769	29.21634400708325
20	24.006976854756473	21.403157738294134	23.705479909790306	30.884385497159084
21	24.435649121127096	27.603328701824243	21.90035506019798	26.060667116850677
22	25.87241439272205	23.998432003096095	22.89438397105523	27.234769633126625
23	29.833136003782347	23.811376223168917	24.35375818867764	22.001729584371102
24	28.073827518345663	21.901651749749558	25.296883722523066	24.727637009381713
25	25.718647822931906	26.537723501207484	22.306396713910075	25.43723196195054
26	25.30158840672973	29.391489855009766	23.87737258052469	21.429549157735813
27	24.949111827333937	24.83836472870025	23.958958548310825	26.25356489565499
28	23.88651685933068	22.381911934370468	22.219011727304984	31.512559478993868
29	23.8976632978891	24.619865689743097	25.10134549144557	26.381125520922232
30	24.093611761871678	25.461477496098343	24.220769018598755	26.22414172343122
31	23.984736313691574	29.55039668188082	23.601136888263152	22.863730116164458
32	26.901124136789278	24.64840971698696	22.476873875134913	25.973592271088847
33	25.699718106397277	22.5017591479275	24.932500311702384	26.866022433972837
34	23.450700353468346	27.477497681403918	26.35693936636133	22.714862598766405
35	22.086342481142673	23.951065785442292	22.766227513459096	31.196364219955942
36	22.431026589123483	23.88759057787813	28.095773062998624	25.585609769999763
37	20.78734031658859	30.541144750551098	22.55557300070263	26.11594193215768
38	24.973448744288394	27.212174284014235	23.24741129934623	24.56696567235114
39	26.065749841426232	28.3586944325968	23.642395089143204	21.93316063683377
40	25.23707285231308	23.766269864419225	25.29670566820591	25.699951615061785
41	26.89585622619927	27.721672494291287	22.646619791820914	22.735851487688528
42	22.57961761170861	31.92314475484434	21.52093703699963	23.97630059644742
43	24.51314846489386	23.598317360714816	23.095550770791025	28.792983403600296
44	24.607402546442966	24.296863624334794	21.95407420162296	29.14165962759928
45	22.930277897578875	23.815171575661477	27.46156522408328	25.792985302676364
46	20.958154233191028	26.38746164755045	31.073966222994624	21.580417896263896
47	25.493383125102646	29.449904359323597	23.369629232701314	21.68708328287244
48	28.82919214710105	27.072519720900157	19.605043126975133	24.49324500502366
49	21.376735433903043	26.88797700651521	22.94963496618934	28.785652593392406
50	19.638920375518268	30.99208429291333	27.62473909201182	21.744256239556584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11933.0
1	9862.0
2	5734.5
3	3936.5
4	4379.5
5	4784.0
6	4923.0
7	4507.0
8	3731.0
9	3116.5
10	2861.5
11	3203.0
12	3835.0
13	4184.0
14	4462.5
15	5663.5
16	6662.0
17	9475.0
18	12441.5
19	10328.5
20	8082.0
21	11379.5
22	15192.0
23	15672.0
24	16697.5
25	24562.0
26	34233.5
27	37703.5
28	40301.0
29	47216.5
30	58730.5
31	73338.5
32	81480.0
33	73044.5
34	68856.5
35	85322.5
36	97208.5
37	99396.5
38	103409.0
39	98420.5
40	97112.0
41	118755.0
42	142657.5
43	156942.5
44	155061.5
45	148516.0
46	154486.0
47	150409.5
48	147941.5
49	175087.5
50	232759.5
51	254701.5
52	213489.5
53	180317.5
54	172316.0
55	168377.0
56	173054.0
57	181838.0
58	182500.0
59	172640.5
60	160538.5
61	158334.0
62	156366.0
63	146367.5
64	138770.0
65	136292.0
66	160698.5
67	177957.5
68	134121.5
69	98343.5
70	87705.5
71	74042.0
72	67319.5
73	61454.0
74	53502.5
75	44839.0
76	38983.0
77	30173.0
78	22384.5
79	19206.5
80	14726.0
81	10637.5
82	8304.0
83	6770.0
84	5881.5
85	4638.0
86	3062.0
87	2107.0
88	1510.0
89	1141.5
90	1007.5
91	734.5
92	508.5
93	423.5
94	221.0
95	17.5
96	9.0
97	8.5
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	24174.0
25	24734.0
26	21428.0
27	23269.0
28	25667.0
29	24217.0
30	24489.0
31	24899.0
32	23658.0
33	26520.0
34	22964.0
35	22295.0
36	25186.0
37	21969.0
38	25157.0
39	24316.0
40	22744.0
41	25731.0
42	26340.0
43	27445.0
44	25581.0
45	36754.0
46	47014.0
47	109996.0
48	421971.0
49	822255.0
50	1056886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.317790346832151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.94194778000583	6.330688251695124
2	13.417291875377458	2.5003902543404304
3	5.647916129365004	1.5787829516974452
4	2.92564419805871	1.0904215706774774
5	1.760118554539814	0.8200207838385617
6	1.1154997567201013	0.6236395719036104
7	0.8122103575320351	0.529760408030636
8	0.6146756966777754	0.4581935418349202
9	0.47541267137369403	0.3986816040078742
>10	3.3741912488648182	6.307540736944725
>50	0.5315138690870854	3.4902231046470833
>100	0.7249862328508335	16.817894221786887
>500	0.5511465994690464	37.21365335740966
>1k	0.10351846751685459	13.481469499541877
>5k	0.0017848011640836999	1.1598375700510817
>10k+	0.00214176139690044	7.198802571592609
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	74404	2.4738176768044515	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	55940	1.8599182952588709	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	44519	1.480187747347688	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	16983	0.5646584270357776	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	13809	0.4591278466076108	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	10780	0.358418291435299	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	9643	0.32061480373938667	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	7113	0.23649622513722468	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	7022	0.23347061618355006	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	5865	0.1950021594868301	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	5228	0.1738228968111079	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	4290	0.14263585067323123	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	4030	0.13399125366273237	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	3969	0.13196309821026916	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	3824	0.12714207295441404	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	3765	0.12518041440203162	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	3497	0.11626982979120971	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3446	0.11457415883915031	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGTA	3347	0.11128256228515267	No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT	3182	0.10579656802848993	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	3137	0.10430038777667283	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	3130	0.10406764862639017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.04136107185023302	0.0	0.0	0.0	0.0
2	0.06819257103281988	0.0	0.0	0.0	0.0
3	0.08940508215858248	0.0	0.0	0.0	0.0
4	0.11480689798943297	0.0	0.0	0.0	0.0
5	0.15271013103546646	0.0	0.0	0.0	0.0
6	0.2081685457028207	0.0	0.0	0.0	0.0
7	0.257775233163068	0.0	0.0	0.0	0.0
8	0.33205227055327746	0.0	0.0	0.0	0.0
9	0.3929301825772137	0.0	0.0	0.0	0.0
10	0.45340911320066535	0.0	0.0	0.0	0.0
11	0.5164481744772264	0.0	0.0	0.0	0.0
12	0.594681777422241	0.0	0.0	0.0	0.0
13	0.6648692554574838	0.0	0.0	0.0	0.0
14	0.7295374907860233	0.0	0.0	0.0	0.0
15	0.8010549068228812	0.0	0.0	0.0	0.0
16	0.8738357639612735	0.0	0.0	0.0	0.0
17	0.9577216034131529	0.0	0.0	0.0	0.0
18	1.0291060256498492	0.0	0.0	0.0	0.0
19	1.1061759328434506	0.0	0.0	0.0	0.0
20	1.1844095357884654	0.0	0.0	0.0	0.0
21	1.2564589270259694	0.0	0.0	0.0	0.0
22	1.3190657584520054	0.0	0.0	0.0	0.0
23	1.3810076208772337	0.0	0.0	0.0	0.0
24	1.4428829864023813	0.0	0.0	0.0	0.0
25	1.5053235755782155	0.0	0.0	0.0	0.0
26	1.5723856993096623	0.0	0.0	0.0	0.0
27	1.6308697229306912	0.0	0.0	0.0	0.0
28	1.6908831752535776	0.0	0.0	0.0	0.0
29	1.7580450443351456	0.0	0.0	0.0	0.0
30	1.8208846149114644	0.0	0.0	0.0	0.0
31	1.8870157820417808	0.0	0.0	0.0	0.0
32	1.9453335634126077	0.0	0.0	0.0	0.0
33	2.0054135126355748	0.0	0.0	0.0	0.0
34	2.073306847618031	0.0	0.0	0.0	0.0
35	2.131192399138333	0.0	0.0	0.0	0.0
36	2.193433497613925	0.0	0.0	0.0	0.0
37	2.251751278984752	0.0	0.0	0.0	0.0
38	2.3049820474994007	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGG	80	0.0	116.36222	44
CTAACCA	220	0.0	108.428444	44
TACGCGC	120	0.0	96.96852	44
TAGGACA	85	0.0	95.82772	44
CTATCAT	5625	0.0	94.951584	44
GATCGTA	145	0.0	92.287285	44
TATCGTT	90	0.0	90.50395	44
CGACGGG	455	0.0	88.2307	44
TAAGCCA	2880	0.0	80.20105	44
ACGAACA	300	0.0	75.635445	44
ACTTCTA	1520	0.0	74.25748	44
ATAGTGT	150	0.0	73.696075	44
GTTAATG	95	0.0	73.491936	44
TAGCGGG	170	0.0	71.87079	44
TGTGCAC	115	0.0	70.829185	44
AACTACC	390	0.0	70.1157	44
ATCGCAA	265	0.0	68.06093	44
TATACCG	200	0.0	66.90828	44
GGTGCAA	210	0.0	66.49271	44
TCGTCCC	245	0.0	66.4927	44
>>END_MODULE