FastQCFastQC Report
Tue 24 May 2016
ERR841242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659736
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC866473.257729338550894No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT662002.4889688299891417No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA444891.6726848078155123No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA161590.6075415003594342No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC141620.5324588605786439No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC93550.3517266375309429No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA76500.2876225309579597No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA66710.25081436653863387No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG50550.190056456731044No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG49120.18467998327653573No Hit
CGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGAGG42440.15956470867785374No Hit
AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGAT40330.15163159050371916No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA36230.13621652675303114No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA33710.12674190220382775No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC31700.1191847611943441No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG31000.1165529210417876No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA29850.11222918364830195No Hit
CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA29690.11162762018486044No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT29240.10993572294393128No Hit
CACTGTAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAAC28150.10583757184923616No Hit
AATTCGTGGAGAAAGAAATGGCTCC27640.10392008830951643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG700.0129.912444
ATCTAAC750.0121.2515844
TAGGCCG750.0121.2515844
TAGGGCA1200.0119.08636544
TACTAGT1150.0112.9673144
CTATCAT85350.0111.4187244
CTTACGT600.0108.2603344
AAGTATC850.0106.9866844
CGTATGA1750.0103.9299344
ATTAGTC3450.096.0222244
GTCGTGA2100.095.88772644
TCTTATG1300.089.93935444
TAAGCCA23300.089.4890144
AATCGCA10100.082.3207344
ATAAATC3300.078.7347944
TCGACAA1850.077.2452144
CCCATAC15100.073.5596744
CATGACC4900.071.5843844
TAAGTCC1850.070.2229244
GTTAAAC1500.069.2866144
Produced by FastQC (version 0.11.5)