##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841242.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2659736 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6899643423257 39.0 37.0 39.0 32.0 39.0 2 37.22971452805842 39.0 37.0 39.0 34.0 39.0 3 37.260653312960386 39.0 37.0 39.0 33.0 39.0 4 37.75354433673117 39.0 37.0 40.0 33.0 41.0 5 38.22419067155537 40.0 37.0 41.0 34.0 41.0 6 38.11592127940517 40.0 37.0 41.0 33.0 41.0 7 38.06007400734509 40.0 37.0 41.0 33.0 41.0 8 37.906304610683165 39.0 37.0 40.0 33.0 41.0 9 37.848543238877845 39.0 37.0 40.0 33.0 41.0 10 37.84136357894167 39.0 37.0 40.0 33.0 41.0 11 37.63978943774871 39.0 36.0 40.0 32.0 41.0 12 37.97854147930471 39.0 37.0 41.0 33.0 41.0 13 38.105512727578976 39.0 37.0 41.0 34.0 41.0 14 38.10471415208126 40.0 37.0 41.0 34.0 41.0 15 38.057482020771985 40.0 37.0 41.0 33.0 41.0 16 38.122010981541024 40.0 37.0 41.0 34.0 41.0 17 38.04726935304857 40.0 37.0 41.0 33.0 41.0 18 38.12780403769397 40.0 37.0 41.0 33.0 41.0 19 38.097774365576136 40.0 37.0 41.0 33.0 41.0 20 37.91313498783338 39.0 37.0 41.0 33.0 41.0 21 37.837959105715754 39.0 36.0 41.0 33.0 41.0 22 37.81378941368617 40.0 36.0 41.0 33.0 41.0 23 37.73201062060295 39.0 36.0 41.0 33.0 41.0 24 37.47217956970165 39.0 35.0 41.0 32.0 41.0 25 37.4419907696335 39.0 35.0 41.0 32.0 41.0 26 37.411535176863836 39.0 35.0 41.0 32.0 41.0 27 37.369700758461924 39.0 35.0 41.0 32.0 41.0 28 37.343737704458675 39.0 35.0 41.0 32.0 41.0 29 37.275764543257196 39.0 35.0 41.0 32.0 41.0 30 37.26898064122838 39.0 35.0 41.0 32.0 41.0 31 37.0693898674233 39.0 35.0 41.0 31.0 41.0 32 37.03508803759539 39.0 35.0 41.0 31.0 41.0 33 36.95219491754693 39.0 35.0 41.0 31.0 41.0 34 36.87644262432396 38.0 35.0 40.0 31.0 41.0 35 36.876233700562864 38.0 35.0 40.0 31.0 41.0 36 36.82319984821295 38.0 35.0 40.0 31.0 41.0 37 36.792243164156275 38.0 35.0 40.0 31.0 41.0 38 36.62983566707916 38.0 34.0 40.0 31.0 41.0 39 36.57906284073079 38.0 34.0 40.0 31.0 41.0 40 36.57238270979256 38.0 35.0 40.0 31.0 41.0 41 36.462841969351565 38.0 34.0 40.0 31.0 41.0 42 36.25509604098479 38.0 34.0 40.0 30.0 41.0 43 36.25510597233687 37.0 34.0 40.0 30.0 41.0 44 36.16089728287136 37.0 34.0 40.0 30.0 41.0 45 35.9412020609681 37.0 34.0 40.0 30.0 41.0 46 35.74895044271349 37.0 34.0 40.0 30.0 41.0 47 35.696272775312025 36.0 34.0 40.0 30.0 41.0 48 35.78172434820857 37.0 34.0 40.0 30.0 41.0 49 35.92028697326906 37.0 34.0 40.0 29.0 41.0 50 35.96930279890856 37.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 8.0 14 169.0 15 2402.0 16 2599.0 17 67.0 18 0.0 19 1.0 20 3.0 21 12.0 22 43.0 23 147.0 24 354.0 25 854.0 26 2060.0 27 4536.0 28 9368.0 29 19304.0 30 35462.0 31 60569.0 32 97544.0 33 148236.0 34 210083.0 35 223259.0 36 281465.0 37 324098.0 38 364184.0 39 469027.0 40 403882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 39.65698099360237 20.481318446642824 39.8617005597548 2 26.077813737904815 21.36321048404804 29.862399877281053 22.696575900766092 3 29.073562188126946 20.484514252542358 23.7323929893794 26.7095305699513 4 24.12371002234808 23.090374383021473 26.819503890611703 25.966411704018743 5 22.024140741787907 23.840260837917747 25.47264089368268 28.662957526611667 6 23.54808898326751 24.23857104614894 23.094886108997283 29.118453861586264 7 25.19016924988044 21.571727419563448 20.787702238116864 32.45040109243925 8 24.171271133676424 23.924554918232484 26.482026787621027 25.42214716047006 9 26.13691734818794 26.00867153732551 25.413311697100767 22.441099417385786 10 34.56846844950025 19.750982804308396 22.188931533054408 23.49161721313694 11 24.9466864380525 20.98978996411674 24.216087611702815 29.847435986127945 12 27.826145151248095 21.52142167493315 23.28031804660312 27.372115127215636 13 27.504722273188015 21.76998017848388 22.213294853323788 28.51200269500432 14 22.2639013796858 22.784704948160268 20.737170907187778 34.214222764966145 15 24.145779881913093 28.052069829486836 25.116515323325324 22.685634965274748 16 22.909153389659725 28.99220072969648 19.73695885606692 28.361687024576877 17 23.35641582472847 34.109776308626124 18.33215777806519 24.20165008858022 18 23.400517946142024 23.429693774118935 20.05007263878821 33.11971564095083 19 27.959842630997965 22.72838356889556 19.35372533213823 29.95804846796825 20 24.187362956323486 21.493223387584333 23.756530723349986 30.5628829327422 21 23.544705188785652 30.240106536889378 20.73480225105048 25.48038602327449 22 26.89176670165761 22.84331978812935 23.238133408729286 27.026780101483755 23 31.173469848135305 22.388763396066377 24.233232170410897 22.20453458538742 24 26.340245798831162 22.450122869337406 24.174730123591214 27.034901208240214 25 25.289762563712483 26.578599435100053 19.897301265428997 28.23433673575847 26 24.60203341373526 29.989102885898646 23.753680836996317 21.655182863369777 27 25.015368499028558 25.814761698655627 22.25023346628932 26.9196363360265 28 23.909518047860136 22.103194787838547 20.846232908832707 33.14105425546861 29 24.256544882984386 25.217056653268955 25.485429744487924 25.040968719258732 30 24.269380048253435 26.48884015604187 23.723748092524676 25.518031703180018 31 25.186736335208256 29.426153677080315 23.2275983335498 22.15951165416163 32 24.96371412536528 25.19046052419381 22.745598926583195 27.10022642385772 33 25.0719976892393 22.4804877006914 26.077748147424906 26.369766462644396 34 27.01482903659712 27.155498765752462 26.088609611528184 19.74106258612223 35 21.840582593486026 23.080790496796066 22.87118781759552 32.207439092122385 36 21.837484213227373 23.442114052588863 28.59769159980709 26.122710134376675 37 21.446464700917623 30.8854546587848 22.312242776689313 25.35583786360826 38 25.473663103705658 26.66298772993886 21.565366336709374 26.29798282964611 39 25.49706483611037 28.154196688150314 24.31913891564932 22.029599560090006 40 24.063086126163586 25.233280920388633 24.858116859208433 25.84551609423934 41 26.781206097494838 27.788984549817258 21.91407486979053 23.515734482897376 42 20.852872040921177 31.86667308692216 21.954313992019152 25.32614088013751 43 25.34902586168794 22.420025742484967 22.541014528922794 29.6899338669043 44 24.314174457429424 23.1292805572616 21.374796421807247 31.18174856350173 45 23.140607161165715 23.187397546175262 29.54550330705311 24.12649198560591 46 21.13039093950626 27.30783163853391 28.846022721738258 22.715754700221577 47 22.501404018454846 29.59874692833937 26.012089961445774 21.88775909176001 48 28.92649352207294 25.60520633397313 19.870191538707612 25.598108605246324 49 20.232681691421625 29.697615521263486 21.51619341282617 28.553509374488716 50 19.39389335619436 29.866680171071874 29.02699554716353 21.71243092557023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5278.0 1 4111.5 2 2472.0 3 2091.0 4 2217.0 5 2105.5 6 1957.5 7 1935.5 8 2080.0 9 2750.5 10 3640.0 11 4336.5 12 5009.5 13 5861.0 14 6516.5 15 6986.0 16 7580.5 17 9338.5 18 10949.5 19 10864.5 20 10695.0 21 13759.5 22 16543.0 23 17022.5 24 18730.0 25 20886.5 26 23738.0 27 28196.5 28 33367.5 29 39102.0 30 47658.0 31 57518.5 32 59772.5 33 56582.0 34 64848.0 35 80679.0 36 82921.5 37 79796.5 38 82362.5 39 83432.5 40 86633.0 41 95536.0 42 106144.5 43 115448.5 44 124426.5 45 130771.5 46 132594.0 47 124277.0 48 120175.0 49 167916.5 50 256413.5 51 272773.0 52 209240.0 53 164179.0 54 150091.0 55 148955.0 56 158272.5 57 162243.5 58 154184.5 59 138600.5 60 128560.5 61 132067.5 62 132057.0 63 127302.0 64 123503.5 65 119218.5 66 134893.0 67 148018.5 68 120284.5 69 94603.5 70 84900.5 71 76501.5 72 69298.0 73 59645.0 74 49317.5 75 39525.0 76 35254.5 77 30651.0 78 26795.5 79 24704.0 80 19462.0 81 14124.5 82 10449.0 83 6881.5 84 5287.5 85 4397.0 86 3284.5 87 2820.5 88 2153.0 89 1733.0 90 1231.5 91 638.5 92 396.5 93 218.5 94 200.0 95 142.0 96 95.5 97 95.0 98 461.0 99 827.0 100 827.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 23871.0 25 22329.0 26 18943.0 27 25064.0 28 23074.0 29 20210.0 30 23920.0 31 22021.0 32 22239.0 33 22573.0 34 22832.0 35 19834.0 36 18430.0 37 20455.0 38 22555.0 39 22796.0 40 22274.0 41 22647.0 42 23792.0 43 23898.0 44 25358.0 45 34712.0 46 45894.0 47 109375.0 48 368711.0 49 802570.0 50 829359.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.368577509772715 #Duplication Level Percentage of deduplicated Percentage of total 1 68.18742778327058 6.388192023795999 2 13.196757548385552 2.472696919394564 3 5.506545400289498 1.5476549218108373 4 2.920123622166672 1.0942961796954689 5 1.7784768419466541 0.8330899071556513 6 1.1317454391557975 0.6361706920837709 7 0.8218826353504259 0.5389909821251709 8 0.6323737433886647 0.4739553944065461 9 0.501824106595933 0.4231240235024799 >10 3.445961184921859 6.566028611942104 >50 0.5399139007422387 3.5807956813765216 >100 0.7225553924476542 16.537769685314274 >500 0.489046637571278 33.80451667552229 >1k 0.1217609973969439 15.482034035281561 >5k 0.0016021183868018935 1.0780999097597246 >10k+ 0.002002647983502367 8.542584356833025 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 86647 3.257729338550894 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 66200 2.4889688299891417 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA 44489 1.6726848078155123 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 16159 0.6075415003594342 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 14162 0.5324588605786439 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 9355 0.3517266375309429 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 7650 0.2876225309579597 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 6671 0.25081436653863387 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG 5055 0.190056456731044 No Hit CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG 4912 0.18467998327653573 No Hit CGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGAGG 4244 0.15956470867785374 No Hit AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGAT 4033 0.15163159050371916 No Hit CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA 3623 0.13621652675303114 No Hit AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA 3371 0.12674190220382775 No Hit CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC 3170 0.1191847611943441 No Hit AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG 3100 0.1165529210417876 No Hit CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA 2985 0.11222918364830195 No Hit CCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACA 2969 0.11162762018486044 No Hit ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT 2924 0.10993572294393128 No Hit CACTGTAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAAC 2815 0.10583757184923616 No Hit AATTCGTGGAGAAAGAAATGGCTCC 2764 0.10392008830951643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02003958287589445 0.0 0.0 0.0 0.0 2 0.044478098578204756 0.0 0.0 0.0 0.0 3 0.059479587447776774 0.0 0.0 0.0 0.0 4 0.07981995205539197 0.0 0.0 0.0 0.0 5 0.10621354901388709 0.0 0.0 0.0 0.0 6 0.15106762475674276 0.0 0.0 0.0 0.0 7 0.18874053665476573 0.0 0.0 0.0 0.0 8 0.2563788285754676 0.0 0.0 0.0 0.0 9 0.29999217967497527 0.0 0.0 0.0 0.0 10 0.3542832822505692 0.0 0.0 0.0 0.0 11 0.4095895231707207 0.0 0.0 0.0 0.0 12 0.46357984401459396 0.0 0.0 0.0 0.0 13 0.5217435113860924 0.0 0.0 0.0 0.0 14 0.5823134326113569 0.0 0.0 0.0 0.0 15 0.6528091509834059 0.0 0.0 0.0 0.0 16 0.7190939251113644 0.0 0.0 0.0 0.0 17 0.8180887125639538 0.0 0.0 0.0 0.0 18 0.8896371669970252 0.0 0.0 0.0 0.0 19 0.9795333070650621 0.0 0.0 0.0 0.0 20 1.0566462235349674 0.0 0.0 0.0 0.0 21 1.1361277961421734 0.0 0.0 0.0 0.0 22 1.2033525131817593 0.0 0.0 0.0 0.0 23 1.2632080777941872 0.0 0.0 0.0 0.0 24 1.3236276081535912 0.0 0.0 0.0 0.0 25 1.3857390357539245 0.0 0.0 0.0 0.0 26 1.4631151362390853 0.0 0.0 0.0 0.0 27 1.5168046753512379 0.0 0.0 0.0 0.0 28 1.5769234239789212 0.0 0.0 0.0 0.0 29 1.6523068454914323 0.0 0.0 0.0 0.0 30 1.7139671004941843 0.0 0.0 0.0 0.0 31 1.7748002057347045 0.0 0.0 0.0 0.0 32 1.832174321060436 0.0 0.0 0.0 0.0 33 1.8934585988985373 0.0 0.0 0.0 0.0 34 1.9701203427708616 0.0 0.0 0.0 0.0 35 2.0306526662796607 0.0 0.0 0.0 0.0 36 2.0912977829378554 0.0 0.0 0.0 0.0 37 2.1533340151052585 0.0 0.0 0.0 0.0 38 2.205482047842342 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 70 0.0 129.9124 44 ATCTAAC 75 0.0 121.25158 44 TAGGCCG 75 0.0 121.25158 44 TAGGGCA 120 0.0 119.086365 44 TACTAGT 115 0.0 112.96731 44 CTATCAT 8535 0.0 111.41872 44 CTTACGT 60 0.0 108.26033 44 AAGTATC 85 0.0 106.98668 44 CGTATGA 175 0.0 103.92993 44 ATTAGTC 345 0.0 96.02222 44 GTCGTGA 210 0.0 95.887726 44 TCTTATG 130 0.0 89.939354 44 TAAGCCA 2330 0.0 89.48901 44 AATCGCA 1010 0.0 82.32073 44 ATAAATC 330 0.0 78.73479 44 TCGACAA 185 0.0 77.24521 44 CCCATAC 1510 0.0 73.55967 44 CATGACC 490 0.0 71.58438 44 TAAGTCC 185 0.0 70.22292 44 GTTAAAC 150 0.0 69.28661 44 >>END_MODULE