FastQCFastQC Report
Tue 24 May 2016
ERR841240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887057
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT538812.8552926594162233No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC510342.7044228128774064No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA354471.878427625662606No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG111900.5929868573127361No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC99880.5292897882787855No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC96290.510265455680459No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA66570.3527715379026707No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC65430.34673038493272856No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA56820.30110378223869233No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA56340.2985601388829272No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG42100.22309871932856296No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG37910.20089483253553023No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTG37470.19856315945941222No Hit
AATTCGTGGAGAAAGAAATGGCTCC34510.1828773587655275No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA31240.16554878840437784No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGAA29790.1578648657671708No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG29660.15717596235831774No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG25240.1337532464573142No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG24430.12946084829446064No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA23730.12575136840063655No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCA23410.12405560616345981No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGT22960.12167094051743005No Hit
ACAGAAGCGAGTCCGACTGTGCTCGCTGCTCAGCGCCGCACCCGGAGGA22320.1182794160430766No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC21790.11547080983775265No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT20380.1079988574801927No Hit
CTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAAGGTCGA19950.10572017697398647No Hit
ACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC19230.10190471194033883No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAACA19150.10148077138104467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC600.0129.2936944
ACGAACA2050.0126.1401944
CTATCAT61950.0122.6150844
CGAAGAC950.0122.4887644
GGTATTG800.0121.2128444
GATAGGA700.0120.0584344
ACTGTCA1100.0117.5397344
ACGGGAT900.0114.9277344
CGACGGG2250.0106.3081544
ACTACCC503.6379788E-12103.4349544
TTGCCGA503.6379788E-12103.4349544
AAGTCGT251.1424809E-5103.4349544
ACCGGTT950.0102.0739744
AATCGCA3500.0101.587944
TGCGTAC800.096.9702744
TAATATT1350.095.7731144
TGGTCAT1700.091.26613644
ATGTGCG1050.086.1957944
TAAGCCA8050.084.32197644
ATGGGGT2000.084.040944
Produced by FastQC (version 0.11.5)