FastQCFastQC Report
Tue 24 May 2016
ERR841238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4874126
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG1161092.3821501536890923No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1098502.253737387995304No Hit
AAGCAGTGGTATCAACGCAGAGTG843701.7309769997739082No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTGGT747951.5345315242158286No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA636781.3064496075809284No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT636421.3057110136258274No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT618061.268042721915683No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG583491.1971171857272462No Hit
AAGCAGTGGTATCAACGCAGAGTGCA423140.8681351282260656No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG408000.8370731491143233Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGG370380.7598900808062821No Hit
AAGCAGTGGTATCAACGCAGAGTGC340330.6982380020541118No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC339970.697499408099011No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTGG321200.6589899399400015No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA297640.6106530688783999No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC252160.5173440325506562No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA242250.4970121822866294No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGG239460.4912880791345976No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTC211220.4333494866566847No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTT146440.30044360773603307No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCAC143710.29484260357651815No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT143650.2947195045840013Illumina Single End Adapter 2 (100% over 32bp)
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG141150.28959037989580083No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCA133980.27488005029004176No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCACTGCA124870.25618951992623906No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTT122140.25058851576672414No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC113280.23241089787174155Illumina Single End Adapter 2 (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGTATGCCGT88090.18072983751343316Illumina Single End Adapter 2 (95% over 24bp)
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA87260.17902696811695062No Hit
AAGCAGTGGTATCAACGCAGAGTAGATCGGAAGAGCTCGTATGCCGTCTT86140.17672912025663678Illumina Single End Adapter 2 (100% over 27bp)
AAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGC80670.16550659543885407Illumina Single End Adapter 2 (96% over 31bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCACTG78460.1609724492144848No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA78230.16050056974317037No Hit
AAGCAGTGGTATCAACGCAGATCGGAAG75830.15557661004249787No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT74280.15239655273581357No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC71990.1476982745214219No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTAGATCGGAAGAGCTCGTATGCC71980.1476777580226691Illumina Single End Adapter 2 (100% over 22bp)
AAGCAGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA71680.14706226306008505Illumina Single End Adapter 2 (100% over 34bp)
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC70870.14540042666110808No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA68550.140640598950458No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC65370.13411635234706695No Hit
AAGCAGTGGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA64960.13327517589820206Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA63880.13105939403289946Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACT63640.13056699806283217No Hit
AAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA62960.12917187614764164Illumina Single End Adapter 2 (97% over 34bp)
AAGCAGTGGTATCAACAGATCGGAAG62280.1277767542324511No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC61040.12523270838710365No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT57210.11737488936478047No Hit
AAGCAGTGGTATCAACGCAGAGGG54960.11275867714540001No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG53710.11019411480129976No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGAG53710.11019411480129976No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT53360.10947603734495169No Hit
AAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCG53300.10935293835243487Illumina Single End Adapter 2 (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAGCAGTG53130.10900415787363724No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG52370.1074449039684243No Hit
AAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCGTCTTCTG50180.10295179074156063Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA49930.10243887827274059Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC49170.10087962436752763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA2200.0117.6333744
ACGAACA1750.0116.9299244
TAAGCCA30350.0104.7032144
CTATCAT78550.0101.7505344
ATACGCT950.0101.36339644
CGTACAG600.0100.30752644
ACCACTC11350.099.1586344
TAGGGAT1300.092.5915644
GTCGTGT1100.087.54111544
CGACGGG5850.087.44758644
ACGTAAA358.3703344E-785.9778844
TGGTCAT2250.082.9208944
CATCACG1850.078.0772144
AATCGCA13350.077.9919244
CGATTCA600.069.37427543
TTACGCG850.069.37427543
TCGACTA1250.069.37427543
TATCGTG252.9976818E-669.37427543
CCGTTAG800.069.37427543
CGTAATT950.065.72343
Produced by FastQC (version 0.11.5)