FastQCFastQC Report
Tue 24 May 2016
ERR841236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557356
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1150262.5239634560038757No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT860121.8873223860501571No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA709421.556648196893111No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA337110.7397052150413529No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC207810.4559880772974505No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG156060.3424353945577216No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA140740.308819411957284No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC126800.2782315008965725No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG98030.21510279205750002No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC75210.1650298989150727No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG70070.15375142955696242No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA64460.14144166047155413No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC63150.13856718676355323No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT61430.13479306861259027No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG59990.13163334178852826No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC56910.12487503719261783No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG54450.1194771705348452No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA53670.11776565183847829No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG53100.11651492663728708No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA52280.11471563775136286No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC50290.11034907082088825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC47210.10359076622497781No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA46360.10172564969688565No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG45840.10058463723264102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTG1400.0117.1574844
TCGATTG1200.0116.4342744
CGGTTAA1050.0115.7110844
CTATCAT104050.0106.90069644
CGTATGT800.0106.30955544
TGCGTAG1200.0101.2471944
TAAGCCA46350.094.8905844
ATTAGTC7050.091.337944
CCTTATA3350.087.0423744
CCAATAG850.085.7623444
CGACGGG12650.073.9544844
CGTCTAA250.001894346672.8979944
CCGTCGT2200.071.7934744
AGATCGT1750.069.4266544
ACGAACA1750.069.4266544
ACTTCTA26200.068.3998344
CCCATAC25400.066.7274944
CGCATAT1300.064.0401143
CCGCGTA150.003166492864.040143
CGATAAG1000.064.040143
Produced by FastQC (version 0.11.5)