##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841236.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4557356
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.81911573289425	39.0	37.0	39.0	32.0	39.0
2	37.37680027630056	39.0	37.0	39.0	35.0	39.0
3	37.31575896199463	39.0	37.0	39.0	33.0	39.0
4	37.79297996469883	39.0	37.0	40.0	34.0	41.0
5	38.25976070335519	40.0	37.0	41.0	34.0	41.0
6	38.14362933244627	40.0	37.0	41.0	33.0	41.0
7	38.12593069314752	40.0	37.0	41.0	33.0	41.0
8	37.98946801610408	39.0	37.0	41.0	33.0	41.0
9	37.925939733477044	39.0	37.0	40.0	33.0	41.0
10	37.926756654516346	39.0	37.0	40.0	33.0	41.0
11	37.595835392275696	39.0	36.0	40.0	32.0	41.0
12	37.95194296868623	39.0	37.0	41.0	33.0	41.0
13	38.09914059818895	39.0	37.0	41.0	33.0	41.0
14	38.111506320770204	40.0	37.0	41.0	33.0	41.0
15	38.0756324500434	40.0	37.0	41.0	33.0	41.0
16	38.08499270190874	40.0	37.0	41.0	33.0	41.0
17	38.05165144000161	40.0	37.0	41.0	33.0	41.0
18	38.12926354667048	40.0	37.0	41.0	33.0	41.0
19	38.08274907643818	40.0	37.0	41.0	33.0	41.0
20	37.972923554798	40.0	37.0	41.0	33.0	41.0
21	37.92086222801115	40.0	37.0	41.0	33.0	41.0
22	37.861971283349384	40.0	36.0	41.0	33.0	41.0
23	37.7646363373851	39.0	36.0	41.0	33.0	41.0
24	37.52717606436715	39.0	36.0	41.0	32.0	41.0
25	37.45832259303568	39.0	35.0	41.0	32.0	41.0
26	37.51066936987558	39.0	36.0	41.0	32.0	41.0
27	37.43320665031844	39.0	35.0	41.0	32.0	41.0
28	37.42257791665911	39.0	35.0	41.0	32.0	41.0
29	37.38091648780769	39.0	35.0	41.0	32.0	41.0
30	37.33117780867481	39.0	35.0	41.0	32.0	41.0
31	37.087980218156645	39.0	35.0	41.0	31.0	41.0
32	37.09057043078805	39.0	35.0	41.0	31.0	41.0
33	36.988113646636045	38.0	35.0	40.0	31.0	41.0
34	36.90200558959855	38.0	35.0	40.0	31.0	41.0
35	36.90529868628608	38.0	35.0	40.0	31.0	41.0
36	36.84974324447081	38.0	35.0	40.0	31.0	41.0
37	36.77743297858471	38.0	35.0	40.0	31.0	41.0
38	36.64052231168848	38.0	35.0	40.0	31.0	41.0
39	36.62052910375228	38.0	35.0	40.0	31.0	41.0
40	36.602592218708345	38.0	35.0	40.0	31.0	41.0
41	36.49423762177914	38.0	35.0	40.0	31.0	41.0
42	36.21246066463073	37.0	34.0	40.0	30.0	41.0
43	36.17090105355687	37.0	34.0	40.0	30.0	41.0
44	36.20082801697396	37.0	34.0	40.0	30.0	41.0
45	36.01457508580778	37.0	34.0	40.0	30.0	41.0
46	35.803548369090926	37.0	34.0	40.0	30.0	41.0
47	35.7506076323499	36.0	34.0	40.0	30.0	41.0
48	35.76727163269089	36.0	34.0	40.0	30.0	41.0
49	35.87277318191149	37.0	34.0	40.0	30.0	41.0
50	35.90163719247676	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	281.0
15	3188.0
16	3763.0
17	108.0
18	0.0
19	0.0
20	2.0
21	41.0
22	62.0
23	181.0
24	521.0
25	1328.0
26	3209.0
27	7012.0
28	14951.0
29	29897.0
30	56425.0
31	99287.0
32	161814.0
33	249241.0
34	352079.0
35	387007.0
36	488439.0
37	580732.0
38	637722.0
39	770721.0
40	709343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.10958195936416	22.05179933277102	36.838618707864825
2	26.825422459864885	22.502850336905873	28.198521247846337	22.473205955382902
3	28.9776572205463	21.674277804937773	24.09730554295078	25.250759431565147
4	23.622732128014576	23.52339821598313	27.63181107642238	25.222058579579915
5	22.312476795756137	25.105806963511302	26.32412302220849	26.25759321852407
6	24.35339701353153	23.99983674744742	22.135115185208264	29.51165105381278
7	28.272182379432287	20.668409490063976	21.856247350437403	29.203160780066334
8	25.109515254020092	22.613045809895034	25.79502237700983	26.482416559075045
9	26.271965587064077	25.943002916603398	25.31369065747771	22.471340838854807
10	32.60669124817109	20.757079324064215	21.818791422043834	24.81743800572086
11	25.343861660138028	21.031821959925885	25.95739722769079	27.666919152245296
12	27.599753014686584	21.964775189824977	23.45212882206262	26.983342973425824
13	27.664154391274238	22.648000287886223	22.09759342917253	27.590251891667013
14	22.33935641630805	22.340607141509246	20.8943519005318	34.4256845416509
15	25.420441150526752	26.74236991799631	24.87848217255795	22.958706758918986
16	23.40271420534187	27.711967202035563	22.16085379329594	26.724464799326626
17	22.51733241818282	33.11255473568446	19.901078607859468	24.46903423827324
18	24.737040512086395	24.318727788656407	20.849172195457193	30.09505950380001
19	28.427952523349063	23.54393644033953	19.9314251508989	28.09668588541251
20	25.29183588027795	20.38091823416911	24.058949970114252	30.268295915438685
21	24.826017541750083	28.103290592176695	20.687192310629232	26.38349955544399
22	26.803896820875966	22.21046589294319	23.969029410912817	27.01660787526803
23	31.146261121580142	21.651435613105495	25.18131126907795	22.020991996236415
24	26.732671312050233	22.445690000956695	25.186687193188327	25.634951493804742
25	25.19438187107085	28.030351472691173	21.079621726163676	25.6956449300743
26	26.08408484637861	28.701511710621325	23.9564789016091	21.25792454139097
27	24.668435550241107	26.556061957711446	22.92985063010854	25.845651861938908
28	24.678605119460116	21.592370698256627	22.40813725668051	31.320886925602743
29	24.11541655077306	24.909557274602832	24.63326386192323	26.34176231270088
30	25.118095928140377	25.410494329714204	23.026857769688355	26.444551972457063
31	24.537877829300424	29.48140811891063	23.291743578483548	22.688970473305396
32	26.178531888002336	25.42590003487079	22.614389575744948	25.781178501381923
33	24.395377100438182	22.389118433330186	25.027612811207923	28.187891655023712
34	25.630811456337288	27.453328093582087	25.841913585169273	21.07394686491135
35	23.14597757896131	22.71870145821041	22.788878586824328	31.346442376003953
36	22.75887228677341	24.241574138978798	27.374229624113404	25.625323950134387
37	21.420582477829438	30.108198819987862	23.273406400374842	25.197812301807854
38	25.886351529348683	26.324315207224657	22.12912323673187	25.660210026694784
39	25.562188028742362	28.340478312029056	24.005954994997975	22.091378664230607
40	24.896424872541814	25.30329341822736	24.628345108167828	25.171936601063
41	26.938387367862354	26.418072754060407	22.84036008445421	23.803179793623023
42	23.0378007155129	30.856230781532407	21.933034830749136	24.172933672205556
43	24.85202801978166	24.380551787014788	23.536891614173033	27.230528579030516
44	26.0651261659123	22.50711393021411	22.04849776307144	29.379262140802155
45	22.424767883113574	23.813725487602223	28.81125304887809	24.950253580406116
46	21.14924693832204	27.05955472162319	28.74762370424121	23.043574635813556
47	24.638988452725478	29.464930169874094	24.28124345149967	21.614837925900765
48	26.626788340898276	26.401676473643406	21.495758599732447	25.475776585725868
49	22.173865331444137	27.450412935614448	22.14409422624032	28.231627506701095
50	19.849498525306064	29.788883157249497	28.561677908992944	21.799940408451498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12388.0
1	9983.0
2	5876.0
3	4674.0
4	5302.0
5	5162.0
6	4734.5
7	4370.0
8	4360.0
9	4509.5
10	4552.0
11	5514.5
12	6798.5
13	8192.0
14	9675.0
15	10791.0
16	11865.0
17	14404.0
18	16460.5
19	17122.5
20	19149.0
21	19534.5
22	20155.0
23	27952.5
24	34070.5
25	43443.5
26	56638.0
27	61353.5
28	61593.0
29	62489.0
30	68860.5
31	86901.0
32	99768.0
33	97442.0
34	110980.0
35	136047.5
36	142721.0
37	144654.5
38	154578.5
39	163748.5
40	167000.0
41	166394.0
42	170232.5
43	194980.5
44	228003.0
45	234672.0
46	233014.0
47	234675.5
48	238458.0
49	288424.0
50	392675.5
51	420164.0
52	338174.5
53	269906.5
54	259032.5
55	266581.0
56	270881.0
57	271206.5
58	257120.5
59	245893.0
60	244896.5
61	247579.5
62	237496.5
63	212900.5
64	198502.0
65	194697.0
66	224495.0
67	251562.5
68	202077.0
69	152090.0
70	140678.0
71	126981.0
72	110211.0
73	96739.5
74	79662.5
75	60661.5
76	51153.0
77	44865.5
78	41543.5
79	37887.0
80	30875.0
81	24284.5
82	19461.5
83	14644.5
84	11315.0
85	8324.0
86	5627.0
87	4397.5
88	3409.0
89	2813.5
90	1978.5
91	1037.0
92	517.0
93	369.5
94	394.5
95	358.5
96	206.0
97	84.0
98	267.5
99	451.0
100	451.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	37773.0
25	36516.0
26	37325.0
27	35902.0
28	34419.0
29	33400.0
30	42725.0
31	37850.0
32	37863.0
33	35089.0
34	36144.0
35	35204.0
36	36510.0
37	37150.0
38	38109.0
39	36036.0
40	35375.0
41	38299.0
42	41390.0
43	34307.0
44	40301.0
45	53281.0
46	75743.0
47	165711.0
48	596227.0
49	1365001.0
50	1523706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.044042946391775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.69711667130154	6.122556305221457
2	13.384829541977956	2.421059464155639
3	5.636797551647198	1.529383174116398
4	2.950726693711452	1.0674599576396389
5	1.7461212133294397	0.7895997621478582
6	1.1916627857936104	0.6466469647400563
7	0.8425899991416371	0.5334294096906023
8	0.6101712529224109	0.4414732012867169
9	0.4746344243766892	0.38633527061088435
>10	3.5108990805651654	6.412828086245072
>50	0.53219202041537	3.421393054922489
>100	0.7586026028176324	17.02124398645194
>500	0.5621700554750874	36.77330575436687
>1k	0.09639969068465522	12.572785739130618
>5k	0.0031487336153279344	1.781013030220725
>10k+	0.0019376822248171905	8.079486839053065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	115026	2.5239634560038757	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	86012	1.8873223860501571	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	70942	1.556648196893111	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	33711	0.7397052150413529	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	20781	0.4559880772974505	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	15606	0.3424353945577216	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	14074	0.308819411957284	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	12680	0.2782315008965725	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	9803	0.21510279205750002	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	7521	0.1650298989150727	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	7007	0.15375142955696242	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	6446	0.14144166047155413	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	6315	0.13856718676355323	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	6143	0.13479306861259027	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	5999	0.13163334178852826	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC	5691	0.12487503719261783	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	5445	0.1194771705348452	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	5367	0.11776565183847829	No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG	5310	0.11651492663728708	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5228	0.11471563775136286	No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC	5029	0.11034907082088825	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	4721	0.10359076622497781	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	4636	0.10172564969688565	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	4584	0.10058463723264102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.041646954944928594	0.0	0.0	0.0	0.0
2	0.07712805407345838	0.0	0.0	0.0	0.0
3	0.10260335159245844	0.0	0.0	0.0	0.0
4	0.12801282146929052	0.0	0.0	0.0	0.0
5	0.15730612223403218	0.0	0.0	0.0	0.0
6	0.20740095792384883	0.0	0.0	0.0	0.0
7	0.25251483535628994	0.0	0.0	0.0	0.0
8	0.32420113767719705	0.0	0.0	0.0	0.0
9	0.3773679300015184	0.0	0.0	0.0	0.0
10	0.4318732177165883	0.0	0.0	0.0	0.0
11	0.493641488617523	0.0	0.0	0.0	0.0
12	0.5588327969111915	0.0	0.0	0.0	0.0
13	0.6220053908450426	0.0	0.0	0.0	0.0
14	0.683620063914252	0.0	0.0	0.0	0.0
15	0.7558988150146708	0.0	0.0	0.0	0.0
16	0.8219458826565228	0.0	0.0	0.0	0.0
17	0.9121516949740156	0.0	0.0	0.0	0.0
18	0.9805027300917462	0.0	0.0	0.0	0.0
19	1.0623703744012976	0.0	0.0	0.0	0.0
20	1.1316649390567688	0.0	0.0	0.0	0.0
21	1.2042947709154168	0.0	0.0	0.0	0.0
22	1.2700785279885969	0.0	0.0	0.0	0.0
23	1.3310349246361268	0.0	0.0	0.0	0.0
24	1.3877783521849072	0.0	0.0	0.0	0.0
25	1.4440829287859014	0.0	0.0	0.0	0.0
26	1.5148257015690676	0.0	0.0	0.0	0.0
27	1.566829538881755	0.0	0.0	0.0	0.0
28	1.6208301480068708	0.0	0.0	0.0	0.0
29	1.6890714703876546	0.0	0.0	0.0	0.0
30	1.7464731743581146	0.0	0.0	0.0	0.0
31	1.8060691330675067	0.0	0.0	0.0	0.0
32	1.8597625465291718	0.0	0.0	0.0	0.0
33	1.914158121507295	0.0	0.0	0.0	0.0
34	1.9831235479519265	0.0	0.0	0.0	0.0
35	2.039515894742478	0.0	0.0	0.0	0.0
36	2.099046025809702	0.0	0.0	0.0	0.0
37	2.1553944875054745	0.0	0.0	0.0	0.0
38	2.20584040395352	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAGTG	140	0.0	117.15748	44
TCGATTG	120	0.0	116.43427	44
CGGTTAA	105	0.0	115.71108	44
CTATCAT	10405	0.0	106.900696	44
CGTATGT	80	0.0	106.309555	44
TGCGTAG	120	0.0	101.24719	44
TAAGCCA	4635	0.0	94.89058	44
ATTAGTC	705	0.0	91.3379	44
CCTTATA	335	0.0	87.04237	44
CCAATAG	85	0.0	85.76234	44
CGACGGG	1265	0.0	73.95448	44
CGTCTAA	25	0.0018943466	72.89799	44
CCGTCGT	220	0.0	71.79347	44
AGATCGT	175	0.0	69.42665	44
ACGAACA	175	0.0	69.42665	44
ACTTCTA	2620	0.0	68.39983	44
CCCATAC	2540	0.0	66.72749	44
CGCATAT	130	0.0	64.04011	43
CCGCGTA	15	0.0031664928	64.0401	43
CGATAAG	100	0.0	64.0401	43
>>END_MODULE