FastQCFastQC Report
Tue 24 May 2016
ERR841235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3491339
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1296613.713790038721534No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT773412.2152245886177195No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA638821.8297277921164343No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA216750.6208219826261501No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC191410.5482423792132475No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG143760.4117617911065067No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC89170.2554034426333278No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT76860.22014476394300295No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG74010.21198170673200167No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA72420.20742758007744308No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG59550.17056493225092148No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG57960.1660108055963629No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG55550.15910800984951617No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC54960.1574181137953089No Hit
ACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAAC50850.14564612602786495No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC50670.14513056451980172No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG50620.14498735298978416No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT49970.14312560309955577No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA47050.13476204974652992No Hit
ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACC41980.1202404006027487No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC41400.11857914685454493No Hit
ACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC39730.1137958817519582No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC39080.11193413186172983No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA38160.10929903970940663No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG37720.10803877824525204No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA36270.10388564387474261No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC35380.10133647864042994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT550.0141.68544
TACGGAC950.0141.68544
ACGAACA1700.0137.5177944
ACGACAC1100.0135.2447844
CTATCAT88350.0133.0251344
CCGGATA1300.0125.3367344
AACCGTA750.0122.7936644
TAAGCCA31800.0114.2836544
CCCGTAC850.0108.3473544
ATTAGTC7500.0102.9577744
AACGTTT900.0102.3280644
CGACGGG6750.099.7042644
ACCACTC10500.099.17950444
AGACACC6350.091.4816544
ACTTCTA12550.090.3171344
GCCGTCA900.078.7138944
AATCGCA9300.076.9364844
CTTATAA1300.076.2919244
CATAAGG2450.075.1797944
GTAACTC2850.072.0853544
Produced by FastQC (version 0.11.5)