##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841235.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3491339 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.51956341105805 39.0 35.0 39.0 31.0 39.0 2 37.19608293551557 39.0 37.0 39.0 34.0 39.0 3 37.41405231631761 39.0 37.0 39.0 33.0 39.0 4 37.885674808433095 39.0 37.0 40.0 34.0 41.0 5 38.33247845597348 40.0 38.0 41.0 34.0 41.0 6 38.23353189134598 40.0 37.0 41.0 34.0 41.0 7 38.22624872577541 40.0 37.0 41.0 34.0 41.0 8 38.05153037273092 40.0 37.0 41.0 33.0 41.0 9 38.03997005160484 40.0 37.0 41.0 33.0 41.0 10 38.00370975147357 40.0 37.0 41.0 33.0 41.0 11 37.673632093589305 39.0 36.0 41.0 32.0 41.0 12 37.99845761182171 39.0 37.0 41.0 33.0 41.0 13 38.121965813116404 40.0 37.0 41.0 34.0 41.0 14 38.08697093006437 40.0 37.0 41.0 33.0 41.0 15 38.03101589390202 40.0 37.0 41.0 33.0 41.0 16 38.10285108378189 40.0 37.0 41.0 33.0 41.0 17 38.04765736011313 40.0 37.0 41.0 33.0 41.0 18 38.03099040224968 40.0 37.0 41.0 33.0 41.0 19 38.01986744913628 40.0 37.0 41.0 33.0 41.0 20 37.88625051878377 40.0 36.0 41.0 33.0 41.0 21 37.84631770217673 39.0 36.0 41.0 33.0 41.0 22 37.73867676556186 39.0 36.0 41.0 33.0 41.0 23 37.66697304386655 39.0 35.0 41.0 33.0 41.0 24 37.40106274412195 39.0 35.0 41.0 32.0 41.0 25 37.38744675874456 39.0 35.0 41.0 32.0 41.0 26 37.429703828571675 39.0 35.0 41.0 32.0 41.0 27 37.337145424948126 39.0 35.0 41.0 32.0 41.0 28 37.25830215762689 39.0 35.0 41.0 32.0 41.0 29 37.22986905759022 39.0 35.0 41.0 32.0 41.0 30 37.11507829837871 39.0 35.0 41.0 32.0 41.0 31 36.97144902297146 39.0 35.0 41.0 31.0 41.0 32 36.944987436237724 38.0 35.0 41.0 31.0 41.0 33 36.8511260805298 38.0 35.0 41.0 31.0 41.0 34 36.77445967954618 38.0 35.0 40.0 31.0 41.0 35 36.76529612547223 38.0 35.0 40.0 31.0 41.0 36 36.71241411972721 38.0 35.0 40.0 31.0 41.0 37 36.640705281847275 38.0 35.0 40.0 31.0 41.0 38 36.4929356675433 38.0 34.0 40.0 31.0 41.0 39 36.38164746746025 37.0 34.0 40.0 31.0 41.0 40 36.38019716023625 37.0 34.0 40.0 31.0 41.0 41 36.22998282319634 37.0 34.0 40.0 31.0 41.0 42 35.98566979542078 37.0 34.0 40.0 30.0 41.0 43 35.97574733250616 37.0 34.0 40.0 30.0 41.0 44 35.954239934060624 36.0 34.0 40.0 30.0 41.0 45 35.759462495560264 36.0 34.0 40.0 30.0 41.0 46 35.48306934666188 36.0 33.0 40.0 29.0 41.0 47 35.37208314001178 36.0 34.0 39.0 30.0 41.0 48 35.4413636104688 36.0 34.0 39.0 30.0 41.0 49 35.696691934800164 37.0 34.0 39.0 30.0 41.0 50 36.17421191779531 37.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 421.0 15 3709.0 16 2452.0 17 29.0 18 0.0 19 1.0 20 4.0 21 22.0 22 73.0 23 203.0 24 495.0 25 1284.0 26 2940.0 27 6442.0 28 13732.0 29 26936.0 30 49780.0 31 84697.0 32 132273.0 33 197836.0 34 289764.0 35 297192.0 36 370490.0 37 419165.0 38 463212.0 39 576539.0 40 551644.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 39.72481618084064 20.7614614335646 39.513722385594754 2 25.22565124727218 21.342642464681887 29.358277726683085 24.073428561362846 3 28.564456215795715 21.103020932656495 23.680398838382636 26.652124013165153 4 23.384609744284358 23.76045408366246 27.925618222693355 24.92931794935983 5 22.680324081963967 25.19901390268891 25.924552156063907 26.19610985928322 6 22.9814120026729 23.001089266897313 22.852550267963093 31.16494846246669 7 28.655166398908843 21.6378300703541 19.463191629343353 30.24381190139371 8 24.181209558854068 22.67302029393307 26.93290453891759 26.21286560829527 9 26.607900292695724 25.35926760477857 24.691558167224667 23.34127393530104 10 35.336299339594355 20.251055540581994 21.408662980019987 23.003982139803668 11 26.449937975086353 18.84975936166611 25.99358011353237 28.70672254971517 12 27.834363835766162 21.51893585813351 22.77702050703183 27.869679799068496 13 28.38879295307617 21.38090858550258 21.45214200053332 28.778156460887928 14 22.9341521977671 22.056466014901446 20.02641393459644 34.98296785273501 15 24.035620717438206 27.73087918417547 23.927725150722974 24.30577494766335 16 22.440272915348526 29.952519649337976 19.65309011814665 27.954117317166855 17 22.340168055866243 33.43195834033877 19.51448999939565 24.713383604399343 18 22.966489361245067 22.88772301973541 21.599420738003385 32.546366881016134 19 29.74343081551233 21.270005576656978 20.08266742358734 28.90389618424335 20 24.895978305171738 19.42231905867634 23.632566187356773 32.049136448795146 21 23.497202649184167 28.68317857418028 21.626745497930735 26.19287327870482 22 26.570951717951193 22.342717221100557 22.977688502892445 28.108642558055806 23 31.765577619360364 20.568183152652892 26.370971137434662 21.295268090552078 24 26.489578926595215 21.231481675082254 24.535171176445484 27.74376822187705 25 24.9828617301134 25.659845048094 20.564451156652737 28.79284206513986 26 25.78601130696206 29.567400344124934 22.422662793927923 22.223925554985087 27 24.502804728829343 25.359119242792055 22.197299761929802 27.940776266448797 28 23.788936131973742 21.169999432452375 21.30337312472805 33.73769131084583 29 24.833929971295802 24.049080303197403 25.66009222304026 25.456897502466536 30 23.626681067197037 24.972586031505564 25.22250291109164 26.178229990205757 31 24.430878263943562 30.376655883663524 22.604866194936786 22.58759965745613 32 26.33583365902655 23.57363941467505 22.31066274266182 27.779864183636583 33 23.72338560304294 20.29182046681291 27.61635888149046 28.36843504865369 34 24.68102012675635 26.967206927582755 26.842519384191398 21.5092535614695 35 22.29784371220013 21.7101780818228 22.27454129632843 33.71743690964864 36 21.27592194998737 22.668034857287196 27.812768375852485 28.243274816872947 37 21.590996624084163 30.270085998957242 22.1688747307957 25.970042646162895 38 27.75536253621253 25.334388033942183 21.321410593577284 25.588838836268003 39 25.520445000961956 27.957112883301257 24.15582547950512 22.366616636231665 40 26.391419845920964 23.82936314020774 24.350194600859858 25.429022413011438 41 25.803464120665065 28.200268074232287 21.011472310649488 24.98479549445316 42 21.69375736944835 31.889509902540087 21.839247766682433 24.577484961329134 43 24.88900169181955 21.948682595667247 22.737738347618087 30.424577364895118 44 25.620502413730843 21.86975816446965 21.110632418902757 31.39910700289675 45 24.074090645021847 21.628310531199322 30.08900816912034 24.20859065465849 46 21.1242561696686 27.831877712437663 30.615520693487632 20.428345424406107 47 24.21571071917763 31.981889005229608 23.0440122070709 20.758388068521864 48 28.647077753896223 26.481627990240632 19.89563797522128 24.975656280641868 49 21.827167861397065 26.231164347066223 20.065029329506405 31.87663846203031 50 18.062300937460538 26.891206872706206 30.116496058336224 24.92999613149703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4739.0 1 3793.5 2 2609.0 3 2337.0 4 2377.0 5 2458.0 6 2432.0 7 2287.0 8 1983.0 9 2046.5 10 2561.5 11 3791.0 12 5109.5 13 5580.5 14 5598.5 15 5311.0 16 5090.5 17 6558.0 18 8249.0 19 9687.0 20 11398.0 21 12515.0 22 13214.0 23 18296.0 24 24531.5 25 28502.0 26 34700.5 27 40043.5 28 44289.5 29 51578.5 30 57233.5 31 62107.0 32 67528.0 33 66342.0 34 77855.5 35 94975.5 36 97947.0 37 106090.0 38 114612.0 39 115068.0 40 118046.0 41 120599.5 42 131906.5 43 162160.5 44 176310.5 45 169898.0 46 172498.0 47 170827.5 48 177089.5 49 223248.0 50 311690.0 51 338126.0 52 256342.0 53 198976.5 54 199092.5 55 202118.5 56 201377.0 57 196457.0 58 198816.5 59 199442.0 60 187476.0 61 188589.0 62 188522.5 63 179903.5 64 170891.5 65 159679.0 66 191055.5 67 223722.5 68 177720.0 69 130766.0 70 114994.0 71 99761.0 72 88345.5 73 76885.5 74 64920.5 75 51415.5 76 41724.0 77 35018.0 78 33048.0 79 29341.5 80 22727.5 81 15862.0 82 10820.5 83 8770.5 84 7119.0 85 5463.5 86 4030.0 87 3189.0 88 2343.0 89 2025.5 90 1691.5 91 1174.0 92 652.5 93 269.5 94 165.0 95 84.5 96 72.5 97 71.5 98 769.5 99 1468.0 100 1468.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 28329.0 25 25305.0 26 24006.0 27 30723.0 28 28066.0 29 25403.0 30 28324.0 31 28676.0 32 27224.0 33 26036.0 34 26445.0 35 25602.0 36 25534.0 37 26722.0 38 27490.0 39 28115.0 40 27343.0 41 29736.0 42 31494.0 43 29883.0 44 35307.0 45 49750.0 46 60397.0 47 144848.0 48 539226.0 49 1113553.0 50 997802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.29556399584793 #Duplication Level Percentage of deduplicated Percentage of total 1 67.08678889555034 5.565227505589782 2 13.403876629477853 2.22385432744568 3 5.708973204026691 1.4207745769375324 4 2.9350222847631016 0.9739066076996847 5 1.8565261306704561 0.7700465663470353 6 1.1938092482204021 0.5941992610468471 7 0.8637205483723671 0.5015534358486287 8 0.6798610130849743 0.4511864433862729 9 0.5340273576996913 0.39870523091982263 >10 3.641972983493913 6.15071251767287 >50 0.5676827798229293 3.316799470225891 >100 0.8054535013608428 16.300978735127362 >500 0.5970156629621044 35.963500786735224 >1k 0.11940313259242087 14.05096077865316 >5k 0.0037960533483139586 1.982807569101123 >10k+ 0.0020705745536257953 9.334786187263115 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 129661 3.713790038721534 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT 77341 2.2152245886177195 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA 63882 1.8297277921164343 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 21675 0.6208219826261501 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC 19141 0.5482423792132475 No Hit CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG 14376 0.4117617911065067 No Hit AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC 8917 0.2554034426333278 No Hit ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT 7686 0.22014476394300295 No Hit AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG 7401 0.21198170673200167 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA 7242 0.20742758007744308 No Hit CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG 5955 0.17056493225092148 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 5796 0.1660108055963629 No Hit AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG 5555 0.15910800984951617 No Hit CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC 5496 0.1574181137953089 No Hit ACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAAC 5085 0.14564612602786495 No Hit ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC 5067 0.14513056451980172 No Hit CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG 5062 0.14498735298978416 No Hit AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT 4997 0.14312560309955577 No Hit ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA 4705 0.13476204974652992 No Hit ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACACC 4198 0.1202404006027487 No Hit TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC 4140 0.11857914685454493 No Hit ACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC 3973 0.1137958817519582 No Hit ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC 3908 0.11193413186172983 No Hit AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA 3816 0.10929903970940663 No Hit CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG 3772 0.10803877824525204 No Hit CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA 3627 0.10388564387474261 No Hit CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC 3538 0.10133647864042994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02887144445154137 0.0 0.0 0.0 0.0 2 0.05456359293669277 0.0 0.0 0.0 0.0 3 0.07005908048459344 0.0 0.0 0.0 0.0 4 0.09065289850111949 0.0 0.0 0.0 0.0 5 0.11368131252794415 0.0 0.0 0.0 0.0 6 0.15206200257265193 0.0 0.0 0.0 0.0 7 0.18571671212678 0.0 0.0 0.0 0.0 8 0.2401084512274517 0.0 0.0 0.0 0.0 9 0.2824417795006443 0.0 0.0 0.0 0.0 10 0.3223691540695418 0.0 0.0 0.0 0.0 11 0.36430149005868523 0.0 0.0 0.0 0.0 12 0.4105015296423521 0.0 0.0 0.0 0.0 13 0.4549830308658082 0.0 0.0 0.0 0.0 14 0.5013262819794927 0.0 0.0 0.0 0.0 15 0.5523382289717498 0.0 0.0 0.0 0.0 16 0.6034933874940245 0.0 0.0 0.0 0.0 17 0.6598041610969316 0.0 0.0 0.0 0.0 18 0.7154848039677614 0.0 0.0 0.0 0.0 19 0.7723684236907387 0.0 0.0 0.0 0.0 20 0.828192278091586 0.0 0.0 0.0 0.0 21 0.8856201016286301 0.0 0.0 0.0 0.0 22 0.9344838756706237 0.0 0.0 0.0 0.0 23 0.9820873882484629 0.0 0.0 0.0 0.0 24 1.0263397510238907 0.0 0.0 0.0 0.0 25 1.0716805214274523 0.0 0.0 0.0 0.0 26 1.1266451066481944 0.0 0.0 0.0 0.0 27 1.170210054079538 0.0 0.0 0.0 0.0 28 1.2154935398710924 0.0 0.0 0.0 0.0 29 1.274124340260284 0.0 0.0 0.0 0.0 30 1.3271126063667837 0.0 0.0 0.0 0.0 31 1.3781818379710478 0.0 0.0 0.0 0.0 32 1.426071773608922 0.0 0.0 0.0 0.0 33 1.4776565667212493 0.0 0.0 0.0 0.0 34 1.5394953053828344 0.0 0.0 0.0 0.0 35 1.592540856101341 0.0 0.0 0.0 0.0 36 1.6502837450044239 0.0 0.0 0.0 0.0 37 1.703529791864955 0.0 0.0 0.0 0.0 38 1.748011293088411 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCGT 55 0.0 141.685 44 TACGGAC 95 0.0 141.685 44 ACGAACA 170 0.0 137.51779 44 ACGACAC 110 0.0 135.24478 44 CTATCAT 8835 0.0 133.02513 44 CCGGATA 130 0.0 125.33673 44 AACCGTA 75 0.0 122.79366 44 TAAGCCA 3180 0.0 114.28365 44 CCCGTAC 85 0.0 108.34735 44 ATTAGTC 750 0.0 102.95777 44 AACGTTT 90 0.0 102.32806 44 CGACGGG 675 0.0 99.70426 44 ACCACTC 1050 0.0 99.179504 44 AGACACC 635 0.0 91.48165 44 ACTTCTA 1255 0.0 90.31713 44 GCCGTCA 90 0.0 78.71389 44 AATCGCA 930 0.0 76.93648 44 CTTATAA 130 0.0 76.29192 44 CATAAGG 245 0.0 75.17979 44 GTAACTC 285 0.0 72.08535 44 >>END_MODULE