FastQCFastQC Report
Tue 24 May 2016
ERR841234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683014
Sequences flagged as poor quality0
Sequence length24-50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC668483.9719218021953475No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA283211.6827548671609387No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT183321.0892363343382765No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC93780.5572146161588674No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC60850.3615537363325557No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA54370.3230513828167799No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG49400.29352102834557525No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA48710.2894212407026917No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA41700.24776977493948357No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC36440.21651632131402354No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA30150.17914289483034604No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG29630.17605319979512946No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG28200.16755653844828386No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG25840.15353407636537783No Hit
TGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG23400.13903627658474618No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG21900.13012369475239063No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTG21010.12483556286519304No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTT20970.12459789401633023No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG20350.1209140268589566No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG20110.11948801376577972No Hit
CGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC20050.1191315104924855No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA19970.11865617279475987No Hit
TCCGACTGTGGTGTCTTTGCTGAGGGTCACATTGAGCTGCAGGTTGAAT19140.11372454418085648No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT19080.11336804090756228No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT18230.10831757786922748No Hit
AATTCGTGGAGAAAGAAATGGCTCC17630.10475254513628526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGTA450.0147.0030844
GCTATAC800.0147.0030844
ACGTCTC750.0147.0030844
CGGTCGT151.1641679E-4147.0030844
ATACGGT100.00680467147.0030844
CGTTTAT700.0147.0030844
TACGTTG450.0147.0030844
TAATCGT750.0147.0030844
CCACACG100.00680467147.0030844
AATCGCA6200.0144.6320644
CCAAGCG850.0138.3558544
CTATCAT24350.0132.2122244
CGAGCAA450.0130.669444
TATGGGG2100.0126.0026544
ATACAGC1300.0124.3872244
TTAGCCT1500.0122.5025744
TCAATGT256.0350285E-6117.6024744
TCCACTA1150.0115.0458944
GCATTGC1400.0110.2523144
CCTAACT1900.0104.44955444
Produced by FastQC (version 0.11.5)