FastQCFastQC Report
Tue 24 May 2016
ERR841233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508040
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1099832.4397077222029973No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT900421.9973647083876807No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA865971.9209456881482863No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA528491.1723276634635007No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC176060.39054666773143093No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG160750.3565851234682922No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC146610.32521894215668007No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA100720.223423039724581No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG81910.1816975891961917No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC80720.17905786106600652No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA78570.17428860436020976No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG72690.16124524183458885No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT71020.1575407494165979No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT63500.1408594422409739No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG61550.13653383732176289No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT57740.12808227078730444No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC52130.11563783817357433No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG51850.1150167256723543No Hit
AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGA51210.11359703995527991No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC50730.1125322756674741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT400.0124.3455644
CTATCAT111350.0106.924944
TAAGCCA66150.0106.8638744
TTGACGC1450.0102.9066744
ACTTCTA19050.083.54977444
TACGAGC1300.081.30286444
ATTAGTC5650.079.2290344
TCGAGTT1600.069.9443844
CTTACTG2050.066.7220144
CTCCGAA2100.065.13338544
TACCGAC1200.064.7493243
CCCATAC29400.064.287544
TACGGCT1750.063.94914244
TAATTCC7900.062.95977844
TTAAGCC61450.060.3238243
CGAAACA700.060.12437443
GGAGACA21650.058.0087844
AATCGCA8250.057.2743244
ACCACTC15700.056.6287144
CCTTAGC140800.055.18408243
Produced by FastQC (version 0.11.5)