##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841233.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4508040
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.856322703436526	39.0	37.0	39.0	32.0	39.0
2	37.38991202385072	39.0	37.0	39.0	35.0	39.0
3	37.574708742602105	39.0	37.0	39.0	34.0	39.0
4	38.010273644421964	39.0	37.0	40.0	34.0	41.0
5	38.51727180770357	40.0	38.0	41.0	34.0	41.0
6	38.42773466961251	40.0	38.0	41.0	34.0	41.0
7	38.42570540634067	40.0	38.0	41.0	34.0	41.0
8	38.31143534662514	40.0	38.0	41.0	34.0	41.0
9	38.270294850977365	40.0	37.0	41.0	34.0	41.0
10	38.270037532941146	40.0	37.0	41.0	34.0	41.0
11	37.975245561263876	40.0	37.0	41.0	33.0	41.0
12	38.252218037107035	40.0	37.0	41.0	34.0	41.0
13	38.34658476854686	40.0	38.0	41.0	34.0	41.0
14	38.31302495097648	40.0	37.0	41.0	34.0	41.0
15	38.24827264176893	40.0	37.0	41.0	34.0	41.0
16	38.292378062306454	40.0	37.0	41.0	34.0	41.0
17	38.26289163361461	40.0	37.0	41.0	34.0	41.0
18	38.29625491344353	40.0	37.0	41.0	34.0	41.0
19	38.29421478070292	40.0	37.0	41.0	34.0	41.0
20	38.15130988189989	40.0	37.0	41.0	33.0	41.0
21	38.13532821359171	40.0	37.0	41.0	33.0	41.0
22	38.03073752673002	40.0	37.0	41.0	33.0	41.0
23	37.964887844828354	40.0	37.0	41.0	33.0	41.0
24	37.69820986504113	39.0	36.0	41.0	33.0	41.0
25	37.65745765377847	39.0	36.0	41.0	33.0	41.0
26	37.672533001085014	39.0	36.0	41.0	33.0	41.0
27	37.62891842730582	39.0	36.0	41.0	33.0	41.0
28	37.59654067613021	39.0	35.0	41.0	33.0	41.0
29	37.56660565189246	39.0	35.0	41.0	33.0	41.0
30	37.47518580864635	39.0	35.0	41.0	32.0	41.0
31	37.30042741776823	39.0	35.0	41.0	32.0	41.0
32	37.22868147099507	39.0	35.0	41.0	32.0	41.0
33	37.167263249249615	39.0	35.0	41.0	32.0	41.0
34	37.069859007837884	39.0	35.0	41.0	32.0	41.0
35	37.03867395461424	39.0	35.0	41.0	32.0	41.0
36	36.958168121116984	38.0	35.0	41.0	31.0	41.0
37	36.94450215950048	38.0	35.0	40.0	31.0	41.0
38	36.79051806913461	38.0	35.0	40.0	31.0	41.0
39	36.72951406922017	38.0	35.0	40.0	31.0	41.0
40	36.67558078118316	38.0	35.0	40.0	31.0	41.0
41	36.57933045380972	38.0	35.0	40.0	31.0	41.0
42	36.3420182174731	38.0	34.0	40.0	30.0	41.0
43	36.31478146019651	37.0	34.0	40.0	31.0	41.0
44	36.25488175034449	37.0	34.0	40.0	30.0	41.0
45	36.11178165925035	37.0	34.0	40.0	30.0	41.0
46	35.89757661579062	37.0	34.0	40.0	30.0	41.0
47	35.86203984556222	36.0	34.0	40.0	30.0	41.0
48	35.90366682189558	37.0	34.0	40.0	30.0	41.0
49	36.063461014648	37.0	34.0	40.0	30.0	41.0
50	36.338565885973054	38.0	34.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	259.0
15	3274.0
16	3674.0
17	99.0
18	0.0
19	1.0
20	3.0
21	21.0
22	69.0
23	182.0
24	512.0
25	1236.0
26	2732.0
27	6186.0
28	12943.0
29	26817.0
30	50908.0
31	88851.0
32	144665.0
33	222752.0
34	338996.0
35	356911.0
36	473115.0
37	538893.0
38	609910.0
39	783297.0
40	841726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.46862050913479	21.8283999254665	36.70297956539871
2	27.213201302561647	22.34938021845414	28.509773648858484	21.927644830125733
3	27.995625593384265	21.431753045669517	24.52910799371789	26.043513367228332
4	24.911757659648096	22.813040700614902	27.705854428975783	24.569347210761215
5	24.354775024179023	23.491051543464568	25.011668041987207	27.14250539036921
6	24.361230157673845	23.03156138809771	23.725366234549828	28.881842219678617
7	29.367840569293975	21.51442755609977	20.364925777056104	28.752806097550156
8	23.780512151622435	23.31072927480679	27.06961340183317	25.839145171737606
9	26.385347068792647	25.160291390493427	26.683858173396864	21.77050336731706
10	33.03830489525381	20.58262570873373	21.566112989237006	24.81295640677545
11	25.066015385843958	20.19263360573553	27.073561902733783	27.66778910568673
12	27.639306660987923	22.238356358861058	22.992187292038224	27.13014968811279
13	28.363168915981223	21.497191684190913	22.687309784296502	27.45232961553136
14	21.85690455275463	22.79041445949903	21.922831208241274	33.42984977950506
15	23.82507697358497	26.780441167336583	26.913226147061692	22.48125571201675
16	22.845560376571637	28.676764181329357	20.629608432933157	27.848067009165845
17	23.885080877720693	33.05806958234621	19.254487537821316	23.80236200211178
18	23.527386624785937	23.419224319216333	20.772952325179013	32.280436730818714
19	27.901371771324122	22.512577528149706	20.77814305108207	28.807907649444104
20	25.90722353838919	19.92058633020115	23.871194576800562	30.300995554609102
21	24.318861412054908	26.740379411007886	21.590380741963248	27.35037843497396
22	25.86212633428275	23.97452107789638	23.643800853586036	26.519551734234838
23	31.809256350875327	21.71537963283378	25.55267921313919	20.922684803151704
24	26.595527102687644	22.76264185765876	25.112975927454062	25.52885511219954
25	25.223360511059216	27.901318311853007	21.213998003451508	25.661323173636262
26	25.49628504997341	28.060436665576628	24.247878192220448	22.195400092229516
27	24.36615857871286	26.356105550005775	23.132997628318826	26.14473824296254
28	24.058126674647966	21.779054785930107	22.486517302320276	31.676301237101644
29	24.997479724700973	24.126486359627094	25.039756051129324	25.836277864542605
30	23.853380128385716	25.1231053115345	24.061543172784116	26.96197138729567
31	24.805825152192988	29.53383117930567	23.415388717998447	22.244954950502898
32	26.64334954392723	24.408059518119064	23.61705867727382	25.331532260679886
33	25.81597432768396	21.05239346784707	24.923705962162327	28.20792624230664
34	25.19513502512462	25.918441251195556	27.223927772570356	21.662495951109467
35	22.77204941843306	22.760195701967692	22.78277076767511	31.68498411192414
36	21.50934351578072	24.004768408251124	29.117572492627374	25.368315583340777
37	22.33873755141726	29.95500347106289	21.19032488952816	26.51593408799169
38	26.15388260041157	26.470419122783944	22.34826321960113	25.027435057203355
39	25.79261223162247	26.95982757543761	25.242972855474484	22.004587337465438
40	25.754002456487672	25.835751556005537	24.003297063129725	24.406948924377065
41	28.012027684936413	26.4445560931285	22.06374014935894	23.479676072576144
42	22.739477250399375	30.372092821121242	21.948499120212265	24.939930808267118
43	24.88556218898277	22.45989898687284	23.01948464503359	29.6350541791108
44	25.636257971336867	23.434986316948656	22.42529670108185	28.503459010632625
45	23.309316062012066	23.570893178567093	28.112928681900478	25.006862077520363
46	22.10938484768027	27.102773911341043	28.23092416330756	22.556917077671127
47	24.1480014095686	30.343845816907578	24.169408409328184	21.33874436419564
48	26.274730962173326	26.589789933789493	20.963683351468987	26.171795752568194
49	21.449917508866562	28.461769699433077	21.786762184201503	28.30155060749886
50	18.73414659525962	31.35213917076473	26.879806698261987	23.033907535713656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8996.0
1	7081.0
2	5021.5
3	5080.5
4	5299.0
5	4433.5
6	3331.5
7	2953.5
8	2863.0
9	2892.0
10	2876.5
11	3780.0
12	4858.5
13	5575.5
14	6998.5
15	9807.5
16	12304.5
17	15098.0
18	18123.0
19	17968.5
20	17150.0
21	23395.5
22	30344.0
23	33039.5
24	35677.0
25	41960.0
26	49713.0
27	54543.0
28	64299.0
29	74735.5
30	82299.5
31	97086.0
32	108585.5
33	105598.0
34	113459.0
35	134941.5
36	135801.5
37	124187.5
38	124731.0
39	136404.5
40	150037.5
41	165956.5
42	180625.0
43	206964.5
44	233863.0
45	224488.0
46	214274.0
47	217649.5
48	227564.5
49	281447.5
50	380078.5
51	412942.5
52	348808.5
53	284328.0
54	258403.0
55	259051.0
56	264884.0
57	250459.5
58	234415.5
59	240790.5
60	241572.5
61	232927.0
62	225466.0
63	218259.5
64	215128.5
65	209271.5
66	234584.5
67	260069.5
68	205205.5
69	150155.0
70	134503.5
71	117945.5
72	98876.0
73	84063.5
74	77951.0
75	70654.5
76	62465.5
77	50827.0
78	42045.5
79	35828.0
80	27765.0
81	19688.5
82	13410.5
83	10938.5
84	8883.0
85	6925.0
86	5177.0
87	3618.5
88	2690.0
89	2280.0
90	1688.0
91	916.5
92	710.0
93	655.5
94	709.0
95	607.5
96	424.0
97	345.0
98	551.5
99	721.5
100	685.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	32316.0
25	32461.0
26	28673.0
27	29340.0
28	30567.0
29	31149.0
30	33586.0
31	34706.0
32	33418.0
33	35492.0
34	35735.0
35	32661.0
36	32732.0
37	32907.0
38	33844.0
39	33088.0
40	34480.0
41	39146.0
42	39767.0
43	41686.0
44	41346.0
45	60270.0
46	85002.0
47	173524.0
48	635900.0
49	1354278.0
50	1479966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.901897430333722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8288654142257	6.038056027333476
2	13.510745386111964	2.4054253926904656
3	5.5841153699130235	1.4912766678634732
4	2.903887334453697	1.03400428802208
5	1.7457374333394307	0.7770187785940832
6	1.1853290999257886	0.6331006841237495
7	0.8051631638230315	0.501723592932493
8	0.6048201870674568	0.43072378152558033
9	0.4986509344149999	0.39950455245321576
>10	3.3894855755744318	6.092515715955622
>50	0.5196917696691159	3.285778603374952
>100	0.755950902530955	16.745493585423397
>500	0.5550113797576927	35.89902964332717
>1k	0.10756613551158511	13.732337157064855
>5k	0.002987948208655142	1.714755064063317
>10k+	0.001991965472436761	8.819256465252097
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	109983	2.4397077222029973	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	90042	1.9973647083876807	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	86597	1.9209456881482863	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	52849	1.1723276634635007	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	17606	0.39054666773143093	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	16075	0.3565851234682922	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	14661	0.32521894215668007	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10072	0.223423039724581	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG	8191	0.1816975891961917	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	8072	0.17905786106600652	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7857	0.17428860436020976	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	7269	0.16124524183458885	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	7102	0.1575407494165979	No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT	6350	0.1408594422409739	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	6155	0.13653383732176289	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	5774	0.12808227078730444	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	5213	0.11563783817357433	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	5185	0.1150167256723543	No Hit
AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGA	5121	0.11359703995527991	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	5073	0.1125322756674741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0284824446988048	0.0	0.0	0.0	0.0
2	0.059183148330538324	0.0	0.0	0.0	0.0
3	0.07870382694031108	0.0	0.0	0.0	0.0
4	0.10294939707722203	0.0	0.0	0.0	0.0
5	0.13413811767419986	0.0	0.0	0.0	0.0
6	0.18027790347911732	0.0	0.0	0.0	0.0
7	0.2253085598175704	0.0	0.0	0.0	0.0
8	0.2936974827197629	0.0	0.0	0.0	0.0
9	0.3459596631795636	0.0	0.0	0.0	0.0
10	0.39709053158357066	0.0	0.0	0.0	0.0
11	0.4549205419650225	0.0	0.0	0.0	0.0
12	0.5161444885138552	0.0	0.0	0.0	0.0
13	0.581228205605984	0.0	0.0	0.0	0.0
14	0.6436721945679276	0.0	0.0	0.0	0.0
15	0.7154106884588424	0.0	0.0	0.0	0.0
16	0.7803169448363368	0.0	0.0	0.0	0.0
17	0.8577563641848786	0.0	0.0	0.0	0.0
18	0.9235499241355445	0.0	0.0	0.0	0.0
19	0.9997249358923168	0.0	0.0	0.0	0.0
20	1.0772309030088465	0.0	0.0	0.0	0.0
21	1.1490581272570786	0.0	0.0	0.0	0.0
22	1.211502116219022	0.0	0.0	0.0	0.0
23	1.272903523482489	0.0	0.0	0.0	0.0
24	1.3292029352002201	0.0	0.0	0.0	0.0
25	1.3863896504911226	0.0	0.0	0.0	0.0
26	1.450452968474104	0.0	0.0	0.0	0.0
27	1.507373492693055	0.0	0.0	0.0	0.0
28	1.563983460661396	0.0	0.0	0.0	0.0
29	1.629777020612062	0.0	0.0	0.0	0.0
30	1.690623863142297	0.0	0.0	0.0	0.0
31	1.7536667820161311	0.0	0.0	0.0	0.0
32	1.8131826691866089	0.0	0.0	0.0	0.0
33	1.8731643907330016	0.0	0.0	0.0	0.0
34	1.945634910071783	0.0	0.0	0.0	0.0
35	2.0032209119706126	0.0	0.0	0.0	0.0
36	2.06335791164231	0.0	0.0	0.0	0.0
37	2.121897764882299	0.0	0.0	0.0	0.0
38	2.1712762087292927	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	40	0.0	124.34556	44
CTATCAT	11135	0.0	106.9249	44
TAAGCCA	6615	0.0	106.86387	44
TTGACGC	145	0.0	102.90667	44
ACTTCTA	1905	0.0	83.549774	44
TACGAGC	130	0.0	81.302864	44
ATTAGTC	565	0.0	79.22903	44
TCGAGTT	160	0.0	69.94438	44
CTTACTG	205	0.0	66.72201	44
CTCCGAA	210	0.0	65.133385	44
TACCGAC	120	0.0	64.74932	43
CCCATAC	2940	0.0	64.2875	44
TACGGCT	175	0.0	63.949142	44
TAATTCC	790	0.0	62.959778	44
TTAAGCC	6145	0.0	60.32382	43
CGAAACA	70	0.0	60.124374	43
GGAGACA	2165	0.0	58.00878	44
AATCGCA	825	0.0	57.27432	44
ACCACTC	1570	0.0	56.62871	44
CCTTAGC	14080	0.0	55.184082	43
>>END_MODULE