FastQCFastQC Report
Tue 24 May 2016
ERR841232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5680545
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1800303.1692381628875403No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA1254382.2082036142658845No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT1113821.960762567676165No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA397810.7003025237895307No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC348900.6142016303013179No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC224080.3944691926566905No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA211820.3728867564643885No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG180280.31736391490605215No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA127110.22376374097907858No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG116240.20462825309895438No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG102910.1811621948246163No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG97650.17190251991666292No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT89950.15834748250387948No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA89510.1575729089374347No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA77970.13725795676295144No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC77550.13651859108589054No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG75470.13285697058996981No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG74460.13107897217608522No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC74220.130656477503479No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT73650.12965305265603916No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC72110.12694204517348248No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC71940.1266427781137197No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC71590.1260266400495023No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT67850.11944276473472176No Hit
TCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA67070.11806965704875148No Hit
AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGAT66540.11713664798007938No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG60710.1068735482246862No Hit
AAGGTATTGGAAAAACCATTTCATAACTTTGTCAAAGTTAAATTATAGGC59450.10465545119350345No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC58680.10329994745222508No Hit
AGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCA58560.10308870011592197No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC57220.1007297715272038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA700.0132.0591944
ATCCGAC100.0093774535132.0591944
CGAATGC700.0122.62639644
CTATCAT131500.0118.6022144
ACGAACA850.0108.7546344
CGATTAG1050.0106.9050644
GGTCGAT2050.0103.0705944
TAAGCCA61200.099.2601844
AATCGCA23050.091.66798444
ACCACTC13350.089.0286644
ACTTCTA36650.082.6946344
CGACGGG9100.078.3647944
CGTATCT1200.071.5320644
ATTAGTC14300.068.80006444
CCCATAC46500.068.15958444
GGCGTTA1350.063.58405344
ACTCGTC1900.062.5543544
TAATCGA1000.062.2674443
TAATGCG900.061.90330543
TGCACTA1650.059.58606743
Produced by FastQC (version 0.11.5)