FastQCFastQC Report
Tue 24 May 2016
ERR841231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145328
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC414833.6219318832683736No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA259592.266512300406521No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT226521.9777740524984981No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC95110.8304171381473255No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA75390.6582393864465026No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT45290.39543257477334004No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA41690.36400053085229733No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG33190.2897859827053909No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC25590.22342944553874525No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG25510.2227309556738332No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA23980.20937233700739002No Hit
CTTTTTGTCCGACATCTTGACGAGGCTGCGGTGTCTGCTGCTATTCTCC21180.1849251917354679No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACAC20910.1825677884413897No Hit
AATTCGTGGAGAAAGAAATGGCTCC19550.1706934607378847No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC18850.16458167441990415No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT18660.16292276099073802No Hit
CACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACG18420.16082729139600183No Hit
CCGCCCAGGACCCGCAGCAGAGACGACGCCTGCAGCAAGGAGACCAGG17020.14860371876004078No Hit
AACTGACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTAT16380.1430157998407443No Hit
CTCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGTGACAGA16140.1409203302460081No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC15870.13856292695192993No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG15690.1369913247558778No Hit
CCCAGGACCCGCAGCAGAGACGACGCCTGCAGCAAGGAGACCAGGAAGG15060.1314907170696953No Hit
CCTTTTCCGGTTGCGGCGCCGCGCGGTGAGGTTGTCTAGTCCACGCTCG15040.1313160946034673No Hit
AGACCATCCAAGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGATG14650.127910956512021No Hit
CACTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAA14420.12590279815039884No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG14190.12389463978877667No Hit
ACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGACAC13730.11987832306553231No Hit
TCCGCAGCCAATTAAGCCGACTGAGTTCCTTTCCTCATGGGGACCCAGTG13720.1197910118324183No Hit
ATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACC13620.11891789950127823No Hit
CTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCCAA13510.11795747593702414No Hit
AGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC13440.11734629730522611No Hit
TGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTC13330.116385873740972No Hit
TTTGGAGATCGTTTCCCTGCCATGTCTGATGCCTACGACCGGACTATG13180.1150762052442619No Hit
AGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAACCGGGAC13050.11394115921377981No Hit
TTTTTTGGGTTTTTGTTTTGTTTTGAGGCAGTCTCGCTCTGTTGTACAA12950.11306804688263974No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT12880.11245686825084168No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC12630.11027408742299148No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG12540.10948828632496542No Hit
ACCCATTCACACGGAAACACCAGATCCAATCATGCCTCGCACTCTCTTT12420.10844055152759732No Hit
ATCTACCCTGCCTGTACTACTCTAGGGAGTATGCTGGAGGCAGAGGGCA12390.10817861782825532No Hit
ATCTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAAGA12350.10782937289579929No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC12240.10686894933154518No Hit
CTCTGAGAAGCACAGCTACCTGCCTTCACTGAAATATACCTCAGGCTGAA12060.10529734713549306No Hit
TATTCAACTAGGTACTACCCAGAATGAACCCATGTACCTGAGTGGTACTC11970.10451154603746698No Hit
CTCTCTCTTTCTGTCTCTTCCTCGCTCCCTCTCTTTCTCTCCTCCCTCT11950.10433692357123898No Hit
AGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCTGGGCATG11930.10416230110501096No Hit
TTTAACCAAGTCCCAGGAATTCACTGCAACCCCTTGCAGGGGGCCATGT11870.10363843370632692No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC11830.10328918877387089No Hit
TTTGTTATAAATCAGAAATGGGTCCATGATGACCACTGTTTTCCAAACC11830.10328918877387089No Hit
TTAAGTCACTCGCCTGAAACCACCCAATCAGTCTACCTCAAAGGCAAG11800.10302725507452887No Hit
TCTCGTCGCAGGCGTCAGAACAAGGCAGTTTTTGCAGTACTGTGTGAAG11710.10224145397650279No Hit
CGTCACATAACCAGTGTCAGCCATCACTTCCAGAGCTCCTGCTCAGTTC11670.10189220904404676No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTT11600.10128103041224873No Hit
ATAGCTAAATGAGTTCCCTCTGGGGAGAGTTCTGGTACTGCAATCACAAT11490.10032060684799463No Hit
ATCAGCAAGCTCCAGTGCTACGTGTCCCTGGCATTTTAGGTGTCGGTTG11480.10023329561488063No Hit
AGCAGAGGCTCATCTACAGTGGCAAGCAGATGAATGATGAGAAGACAGCA11460.10005867314865262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA351.6370905E-11139.388144
ATTAACT750.0139.388144
GCCATAG1450.0139.388144
GTTCCTA650.0139.388144
GATTATC1000.0139.388144
TATGTCC650.0139.388144
TAATGTC850.0139.388144
TACCGAC1000.0139.388144
AACGTAA600.0139.3880944
GGTACTC1350.0139.3880944
TAGAACT202.5904774E-6139.3880944
ATTGGAT600.0139.3880944
AAATGCG202.5904774E-6139.3880944
ACGACAC2350.0133.456744
GAAGCGC1000.0132.418744
ATGCCCA1000.0132.418744
ATCTAAC950.0132.0518844
GCTAGCC950.0132.0518844
GTGTTAA900.0123.9005344
GACTAAC1250.0122.6615344
Produced by FastQC (version 0.11.5)