FastQCFastQC Report
Tue 24 May 2016
ERR841230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736403
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC878743.2112959969712063No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT630652.3046678431502965No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA456571.6685042371317385No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC171060.6251272199306901No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA112510.4111601982602709No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG104400.3815227508521223No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA100070.36569905821620574No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC88510.32345381875403584No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA78960.2885539885755132No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA69890.25540828598711524No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG69230.25299636055069374No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC60120.2197044806631187No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA50600.1849142834589788No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG47530.17369517574713958No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG45410.1659477788907555No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG40010.14621384350185263No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC39360.1438384623902254No Hit
AATTCGTGGAGAAAGAAATGGCTCC38050.13905115584217675No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG35430.12947654274607945No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA33900.12388526105255694No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT33490.12238694373599211No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG32690.11946339775245093No Hit
AGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCAT32210.11770927016232624No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC30670.11208144414400949No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC30520.11153327927209553No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA29020.10605163055295583No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG28550.10433404728762541No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC28090.10265300834708922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC1050.0125.0015344
TTAACGG750.0125.0015244
TATACGA1200.0125.0015244
GTACGCT152.2210195E-4125.0015244
TGCGCTA1300.0120.1937744
TCGACAA1200.0119.7931344
ATCTAGT950.0118.42248544
AGGGTAA550.0113.6377544
AACGTCG451.8189894E-12111.11246544
GTTTGAC3500.0108.929944
CTATCAT76900.0108.8277244
ACGCTTA1100.0107.95586444
GCGAACT1200.0104.1679444
CGTAGAA302.6685848E-7104.1679444
GCTACGG1000.0100.0012244
CGTCAAT251.3514558E-5100.0012244
GTTATTG251.3514558E-5100.0012244
GCCCGAA1450.099.1391444
AATCGCA12850.093.8727444
GCATTCT600.093.75114444
Produced by FastQC (version 0.11.5)