##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841230.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2736403
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.87046790988023	39.0	37.0	39.0	32.0	39.0
2	37.340900079410815	39.0	37.0	39.0	34.0	39.0
3	37.51463691568822	39.0	37.0	39.0	34.0	39.0
4	38.0204359518682	39.0	37.0	40.0	34.0	41.0
5	38.491031109087366	40.0	38.0	41.0	34.0	41.0
6	38.46123432842312	40.0	38.0	41.0	34.0	41.0
7	38.424352334067756	40.0	38.0	41.0	34.0	41.0
8	38.27468870630532	40.0	37.0	41.0	34.0	41.0
9	38.205182496876375	40.0	37.0	41.0	34.0	41.0
10	38.221241534963966	40.0	37.0	41.0	34.0	41.0
11	37.91011667506577	40.0	37.0	41.0	33.0	41.0
12	38.20827195409448	40.0	37.0	41.0	34.0	41.0
13	38.31116578954197	40.0	37.0	41.0	34.0	41.0
14	38.28895963058073	40.0	37.0	41.0	34.0	41.0
15	38.21386615933399	40.0	37.0	41.0	34.0	41.0
16	38.24846961503843	40.0	37.0	41.0	34.0	41.0
17	38.18080450869262	40.0	37.0	41.0	33.0	41.0
18	38.20088269162108	40.0	37.0	41.0	34.0	41.0
19	38.22923450968297	40.0	37.0	41.0	34.0	41.0
20	38.0703321111693	40.0	37.0	41.0	33.0	41.0
21	38.04346764712654	40.0	37.0	41.0	33.0	41.0
22	37.97175269870703	40.0	37.0	41.0	33.0	41.0
23	37.87823138623953	40.0	36.0	41.0	33.0	41.0
24	37.62788595100941	39.0	36.0	41.0	32.0	41.0
25	37.553144213233196	39.0	36.0	41.0	32.0	41.0
26	37.614816739795266	39.0	36.0	41.0	33.0	41.0
27	37.507588772893385	39.0	35.0	41.0	32.0	41.0
28	37.52505483209229	39.0	35.0	41.0	32.0	41.0
29	37.43734628230305	39.0	35.0	41.0	32.0	41.0
30	37.38510912063777	39.0	35.0	41.0	32.0	41.0
31	37.188299687288335	39.0	35.0	41.0	31.0	41.0
32	37.121672355015285	39.0	35.0	41.0	31.0	41.0
33	37.03106891225786	39.0	35.0	41.0	31.0	41.0
34	37.03986681871385	38.0	35.0	41.0	31.0	41.0
35	36.95424302784865	38.0	35.0	41.0	31.0	41.0
36	36.88412878474958	38.0	35.0	40.0	31.0	41.0
37	36.844409369078235	38.0	35.0	40.0	31.0	41.0
38	36.69908023901244	38.0	35.0	40.0	31.0	41.0
39	36.62704226587126	38.0	35.0	40.0	31.0	41.0
40	36.58051155895174	38.0	35.0	40.0	31.0	41.0
41	36.47558831613066	38.0	35.0	40.0	31.0	41.0
42	36.248258924909244	37.0	34.0	40.0	30.0	41.0
43	36.20450874458741	37.0	34.0	40.0	30.0	41.0
44	36.20573058267911	37.0	34.0	40.0	30.0	41.0
45	36.01316476439678	37.0	34.0	40.0	30.0	41.0
46	35.74537380907452	36.0	34.0	40.0	30.0	41.0
47	35.74946217556435	36.0	34.0	40.0	30.0	41.0
48	35.844353205622454	37.0	34.0	40.0	30.0	41.0
49	36.07046330956122	37.0	34.0	40.0	30.0	41.0
50	35.98219669321824	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	208.0
15	2303.0
16	3014.0
17	113.0
18	0.0
19	1.0
20	8.0
21	9.0
22	54.0
23	133.0
24	333.0
25	753.0
26	1794.0
27	3886.0
28	8552.0
29	17435.0
30	32399.0
31	56849.0
32	91417.0
33	141257.0
34	209647.0
35	216465.0
36	286406.0
37	336135.0
38	373846.0
39	462283.0
40	491098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	40.04560000847828	20.662636314899522	39.291763676622196
2	25.779463039618065	20.87634752629638	29.683200902791	23.66098853129455
3	28.22446108997834	20.948266757491496	24.08746080164362	26.73981135088655
4	24.355294158060783	22.156714489788236	27.38887510355748	26.0991162485935
5	20.966758185837392	24.599958412558383	25.973769214549176	28.459514187055053
6	23.66160978481605	23.679297238016474	23.74171494476508	28.91737803240239
7	28.05292202939406	19.821312869485965	20.528847541827723	31.596917559292255
8	24.59659633467731	22.955536885466067	26.187334248646856	26.26053253120977
9	27.66204393139461	25.131276350742198	24.044082688112827	23.16259702975037
10	34.99356637161997	18.942787301431842	20.751841011722323	25.311805315225865
11	25.12323659928746	20.8087039811022	25.622724430575467	28.445334989034876
12	27.110334259975595	21.66592420780126	21.899442443236612	29.32429908898653
13	28.483048732222553	20.92279536310989	20.891367243786828	29.702788660880724
14	22.25830771271629	23.526761226325217	19.772306929936857	34.44262413102164
15	24.46408661297331	28.367093589650356	24.505674054589182	22.663145742787155
16	22.821346124821527	29.178012156835088	20.48806407535732	27.512577642986063
17	22.56962881563863	33.23936569284568	19.745154496614717	24.44585099490097
18	23.234260450671922	23.253300043889734	21.134460092318275	32.37797941312007
19	29.304492064948036	20.911503166748467	19.781296833836244	30.002707934467253
20	24.239594825762143	21.204515562948878	23.051758092649365	31.504131518639618
21	24.3698387993289	28.886644255250417	20.397689960141104	26.34582698527958
22	26.873782845582323	22.616953716247203	21.622838448868826	28.886424989301652
23	30.584128141944007	21.963029568378634	24.323354418190597	23.129487871486766
24	27.353354019857456	21.931455271756388	24.858692232101777	25.85649847628438
25	24.921183570194376	26.646000476401532	19.847728501274705	28.585087452129386
26	25.6249391214604	29.414242492514266	21.320352355692766	23.64046603033257
27	23.05261821372912	26.64106300874442	22.03480164843211	28.27151712909435
28	24.680856844841408	21.96747962570776	20.70302063316915	32.64864289628168
29	25.1890989016336	24.472313518338165	24.78197271076098	25.556614869267257
30	24.03441050890296	24.40943314386957	24.305723842305202	27.250432504922266
31	25.27253180599674	29.040209205570267	22.9127905943993	22.774468394033686
32	25.346452959804715	24.11829947139457	22.554493808393875	27.980753760406834
33	22.64236314626494	21.095600372509317	26.794646967229323	29.467389513996423
34	25.71624762558355	26.1389472449637	26.695018341549854	21.449786787902898
35	22.95904117060618	22.004207565226253	23.36737079118609	31.66938047298148
36	22.481437675926504	23.033078716343507	27.68509432783734	26.800389279892645
37	22.34956007010919	31.462738948110175	21.709913265681404	24.47778771609923
38	26.148942998262616	26.533758109540944	21.05990762633957	26.25739126585687
39	27.551588915006253	27.04532660743453	24.57183816647013	20.83124631108909
40	25.377562928546027	24.943653553666024	23.769017969090424	25.90976554869752
41	26.115928718952176	28.15667890488242	21.940568822391263	23.78682355377414
42	21.93327447929263	31.378115392740213	22.554939765283738	24.13367036268342
43	24.752031508960727	23.99610520919676	21.497964731173923	29.753898550668588
44	25.93850908685356	24.853181320905946	19.59140295429603	29.616906637944464
45	24.019229223923944	23.28737528909482	28.186980842349957	24.506414644631278
46	21.685962815600547	28.129596246317984	28.86990278095626	21.31453815712521
47	23.32977599743543	31.8716290360807	23.500244994956255	21.298349971527614
48	28.24722242759492	27.827141906560648	18.20907431536477	25.71656135047966
49	22.64253906883927	27.679564481046803	19.786318460751044	29.891577989362883
50	20.679399937436813	30.214278913939353	29.49808910267163	19.608232045952207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4537.0
1	3582.0
2	2387.5
3	2225.0
4	2316.5
5	2229.0
6	2090.0
7	2069.5
8	2411.0
9	2979.5
10	3373.0
11	3560.0
12	3651.5
13	4639.5
14	6079.5
15	6476.0
16	6730.0
17	7689.5
18	8645.5
19	10493.5
20	12117.5
21	11922.5
22	12037.5
23	15870.5
24	19740.5
25	20295.5
26	19947.0
27	25314.0
28	34250.0
29	39350.5
30	41290.0
31	48289.0
32	61143.5
33	64832.0
34	66140.5
35	74444.0
36	76373.5
37	72615.0
38	79131.0
39	89641.0
40	89118.0
41	87352.0
42	94375.0
43	108935.0
44	120298.5
45	120068.0
46	126250.0
47	133007.0
48	137333.5
49	179673.0
50	261071.0
51	278153.5
52	212490.5
53	171794.0
54	168117.5
55	172442.0
56	183973.0
57	178813.5
58	158769.0
59	150586.0
60	156744.0
61	156998.0
62	144242.0
63	132958.5
64	129581.5
65	127784.5
66	141639.5
67	156041.0
68	127832.5
69	97525.5
70	88255.5
71	80152.0
72	66668.0
73	52477.0
74	44404.0
75	37940.0
76	35433.5
77	30547.0
78	24943.0
79	21734.0
80	17166.0
81	11779.5
82	7744.5
83	5932.5
84	4786.5
85	3760.0
86	2933.0
87	2034.0
88	1096.5
89	782.0
90	551.5
91	289.5
92	208.5
93	125.0
94	70.0
95	31.5
96	26.0
97	26.5
98	135.0
99	242.5
100	242.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	24405.0
25	22216.0
26	19851.0
27	20023.0
28	19274.0
29	18525.0
30	22503.0
31	22663.0
32	23114.0
33	20051.0
34	21143.0
35	21130.0
36	20772.0
37	20860.0
38	22154.0
39	21827.0
40	20001.0
41	23178.0
42	25502.0
43	22838.0
44	27006.0
45	35728.0
46	51798.0
47	106907.0
48	400711.0
49	799915.0
50	882308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.454527674584817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00537385425702	6.429586891255597
2	13.362954650992725	2.5268084912406525
3	5.735024399249563	1.6266584069137244
4	2.9037782398893	1.098154069195623
5	1.7881298121960556	0.8452961397578878
6	1.1371993069972346	0.6451009391714417
7	0.8074485168547378	0.5343831043883908
8	0.6198449468038529	0.4688272962806866
9	0.4447382864571206	0.3784311393530649
>10	3.3425200954022536	6.42061309457091
>50	0.5466120935083657	3.6563402346088987
>100	0.7040580252452249	16.244947144109897
>500	0.48471687122651963	33.636537490283416
>1k	0.11257191502547263	14.986678952326685
>5k	0.0023210704128963427	1.5262852051072577
>10k+	0.0027079154817124	8.975351401435889
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	87874	3.2112959969712063	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	63065	2.3046678431502965	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	45657	1.6685042371317385	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	17106	0.6251272199306901	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	11251	0.4111601982602709	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	10440	0.3815227508521223	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10007	0.36569905821620574	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	8851	0.32345381875403584	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	7896	0.2885539885755132	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	6989	0.25540828598711524	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	6923	0.25299636055069374	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCGC	6012	0.2197044806631187	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	5060	0.1849142834589788	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG	4753	0.17369517574713958	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	4541	0.1659477788907555	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	4001	0.14621384350185263	No Hit
CTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGGAGAACC	3936	0.1438384623902254	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	3805	0.13905115584217675	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	3543	0.12947654274607945	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	3390	0.12388526105255694	No Hit
ACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAAT	3349	0.12238694373599211	No Hit
CCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG	3269	0.11946339775245093	No Hit
AGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCAT	3221	0.11770927016232624	No Hit
ATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCC	3067	0.11208144414400949	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	3052	0.11153327927209553	No Hit
CCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACA	2902	0.10605163055295583	No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCGGGCTCGG	2855	0.10433404728762541	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	2809	0.10265300834708922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03544799505043665	0.0	0.0	0.0	0.0
2	0.06786281114294934	0.0	0.0	0.0	0.0
3	0.08872962060047442	0.0	0.0	0.0	0.0
4	0.1147491798539908	0.0	0.0	0.0	0.0
5	0.14453280456131645	0.0	0.0	0.0	0.0
6	0.19182116084509482	0.0	0.0	0.0	0.0
7	0.2372457565643657	0.0	0.0	0.0	0.0
8	0.3228691095573276	0.0	0.0	0.0	0.0
9	0.380243699484323	0.0	0.0	0.0	0.0
10	0.4327944385384755	0.0	0.0	0.0	0.0
11	0.49364073932092606	0.0	0.0	0.0	0.0
12	0.5585434601555399	0.0	0.0	0.0	0.0
13	0.6263331826489008	0.0	0.0	0.0	0.0
14	0.6949268802877354	0.0	0.0	0.0	0.0
15	0.765457427140666	0.0	0.0	0.0	0.0
16	0.8317853766422563	0.0	0.0	0.0	0.0
17	0.927458418953641	0.0	0.0	0.0	0.0
18	1.0096831497407364	0.0	0.0	0.0	0.0
19	1.1023961017437856	0.0	0.0	0.0	0.0
20	1.182428173043225	0.0	0.0	0.0	0.0
21	1.2586596345640608	0.0	0.0	0.0	0.0
22	1.3253530273135938	0.0	0.0	0.0	0.0
23	1.3942390795507826	0.0	0.0	0.0	0.0
24	1.458520546863894	0.0	0.0	0.0	0.0
25	1.521632595783589	0.0	0.0	0.0	0.0
26	1.59885075407387	0.0	0.0	0.0	0.0
27	1.6614511824464453	0.0	0.0	0.0	0.0
28	1.7327126157952613	0.0	0.0	0.0	0.0
29	1.8113194584277243	0.0	0.0	0.0	0.0
30	1.8826174361013346	0.0	0.0	0.0	0.0
31	1.9514669440137289	0.0	0.0	0.0	0.0
32	2.01351920751439	0.0	0.0	0.0	0.0
33	2.076740889408468	0.0	0.0	0.0	0.0
34	2.160865925084865	0.0	0.0	0.0	0.0
35	2.22894800217658	0.0	0.0	0.0	0.0
36	2.294252710583931	0.0	0.0	0.0	0.0
37	2.360288305487167	0.0	0.0	0.0	0.0
38	2.4165665656703346	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATC	105	0.0	125.00153	44
TTAACGG	75	0.0	125.00152	44
TATACGA	120	0.0	125.00152	44
GTACGCT	15	2.2210195E-4	125.00152	44
TGCGCTA	130	0.0	120.19377	44
TCGACAA	120	0.0	119.79313	44
ATCTAGT	95	0.0	118.422485	44
AGGGTAA	55	0.0	113.63775	44
AACGTCG	45	1.8189894E-12	111.112465	44
GTTTGAC	350	0.0	108.9299	44
CTATCAT	7690	0.0	108.82772	44
ACGCTTA	110	0.0	107.955864	44
GCGAACT	120	0.0	104.16794	44
CGTAGAA	30	2.6685848E-7	104.16794	44
GCTACGG	100	0.0	100.00122	44
CGTCAAT	25	1.3514558E-5	100.00122	44
GTTATTG	25	1.3514558E-5	100.00122	44
GCCCGAA	145	0.0	99.13914	44
AATCGCA	1285	0.0	93.87274	44
GCATTCT	60	0.0	93.751144	44
>>END_MODULE