FastQCFastQC Report
Tue 24 May 2016
ERR841229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937633
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC803354.146037975199638No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA678133.4997855631071517No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT201851.0417349415498187No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC122360.6314921349915077No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA80650.4162294923754912No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC63920.329887032270817No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG61380.3167782547056125No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA56600.2921089803899913No Hit
AATTCGTGGAGAAAGAAATGGCTCC53350.2753359382297886No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT45410.23435810599840112No Hit
CATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTG42550.21959782889742277No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG41510.21423045540615795No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG32960.17010445218470163No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC31850.16437581316998628No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA28910.14920266118506445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC28550.14734472420731892No Hit
CTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAAGGTCGA28220.14564161531105219No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA26490.13671319594577508No Hit
AAGGTATTGGAAAAACCATTTCATAACTTTGTCAAAGTTAAATTATAGGC25730.1327908845483123No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA25180.12995236972120106No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG24850.12824926082493435No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG24290.12535913663733017No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC23060.11901118529669963No Hit
AATTCGTGGAGAAAGAAATGGCTC22800.11766934192388341No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT22240.11477921773627925No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC22070.11390185860789942No Hit
CTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTTCAGTCGC21100.1088957506400851No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG20770.10719264174381836No Hit
CTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGCTATTC20760.10714103238332544No Hit
CGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGAGG19570.10099951848466661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA800.0205.2694944
CGAATAT1150.0196.3447444
ACCACGA1150.0196.3447444
CTTGCGT600.0188.1637144
CCCATAC8600.0173.0469544
CTATCAT25050.0169.623944
CTTATTA800.0166.7814644
TATACTT1300.0165.7945944
AATCGCA7200.0162.5050244
AGGGCGA650.0157.8996144
ACTTCTA11050.0143.9672944
GTAACAG800.0141.1227744
ATTAGTC4950.0140.993244
ACAAATC1100.0139.9564844
ACCTGAC1400.0139.2900144
CATAATA1050.0136.8463344
ATTACCA2150.0124.1164344
TCAACCG2400.0124.0169944
AGACGAT2450.0121.4860244
CTGACGA2950.0114.8117644
Produced by FastQC (version 0.11.5)