##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841229.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1937633
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.348468466422695	39.0	35.0	39.0	31.0	39.0
2	37.08446439547634	39.0	37.0	39.0	33.0	39.0
3	37.48391620084918	39.0	37.0	39.0	33.0	40.0
4	38.02853997635259	39.0	37.0	40.0	34.0	41.0
5	38.37825687320561	40.0	37.0	41.0	34.0	41.0
6	38.27372417790159	40.0	37.0	41.0	34.0	41.0
7	38.1778417275098	40.0	37.0	41.0	33.0	41.0
8	38.09665917126721	40.0	37.0	41.0	33.0	41.0
9	38.00117927388726	40.0	36.0	41.0	33.0	41.0
10	37.998960071386065	40.0	37.0	41.0	33.0	41.0
11	37.63757636250002	39.0	36.0	41.0	32.0	41.0
12	37.97826575001561	39.0	36.0	41.0	33.0	41.0
13	38.067255254219965	40.0	37.0	41.0	33.0	41.0
14	38.09770322862998	40.0	37.0	41.0	34.0	41.0
15	37.9643916056343	40.0	36.0	41.0	33.0	41.0
16	38.08560909109207	40.0	36.0	41.0	34.0	41.0
17	38.015308368509416	40.0	36.0	41.0	33.0	41.0
18	38.045480748934395	40.0	36.0	41.0	33.0	41.0
19	38.02139930523479	40.0	36.0	41.0	33.0	41.0
20	37.8805491029519	40.0	36.0	41.0	33.0	41.0
21	37.82319407235529	39.0	36.0	41.0	33.0	41.0
22	37.72142351002486	39.0	35.0	41.0	33.0	41.0
23	37.635080017732975	39.0	35.0	41.0	33.0	41.0
24	37.27911529169869	39.0	35.0	41.0	32.0	41.0
25	37.25297263149569	39.0	35.0	41.0	32.0	41.0
26	37.292875467993035	39.0	35.0	41.0	32.0	41.0
27	37.259414033239544	39.0	35.0	41.0	32.0	41.0
28	37.17193995510453	39.0	35.0	41.0	32.0	41.0
29	37.197118366250166	39.0	35.0	41.0	32.0	41.0
30	37.06267700968655	38.0	35.0	41.0	32.0	41.0
31	36.897177191475606	38.0	35.0	41.0	31.0	41.0
32	36.81827335588162	38.0	35.0	40.0	31.0	41.0
33	36.801672232271486	38.0	35.0	40.0	31.0	41.0
34	36.755765124813536	38.0	35.0	40.0	31.0	41.0
35	36.728317895890314	38.0	35.0	40.0	31.0	41.0
36	36.649935739719204	38.0	35.0	40.0	31.0	41.0
37	36.5581324315729	38.0	35.0	40.0	31.0	41.0
38	36.39752717410106	37.0	34.0	40.0	31.0	41.0
39	36.36168832678493	37.0	34.0	40.0	31.0	41.0
40	36.32063773146791	37.0	34.0	40.0	31.0	41.0
41	36.16629512672479	37.0	34.0	40.0	31.0	41.0
42	35.947595619932414	36.0	34.0	40.0	30.0	41.0
43	35.90996859018849	36.0	34.0	40.0	30.0	41.0
44	35.94973663521773	36.0	34.0	40.0	30.0	41.0
45	35.758158223213805	36.0	34.0	40.0	30.0	41.0
46	35.525850578393936	36.0	34.0	40.0	30.0	41.0
47	35.514112571327864	36.0	34.0	39.0	30.0	41.0
48	35.642255343574575	36.0	34.0	40.0	30.0	41.0
49	36.12092304122009	37.0	34.0	40.0	30.0	41.0
50	36.55075804381921	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	190.0
15	2047.0
16	1335.0
17	28.0
18	0.0
19	0.0
20	3.0
21	11.0
22	33.0
23	114.0
24	315.0
25	818.0
26	1815.0
27	3812.0
28	7878.0
29	15264.0
30	27547.0
31	46086.0
32	72871.0
33	113727.0
34	177984.0
35	160883.0
36	207173.0
37	220730.0
38	248478.0
39	298280.0
40	330207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	36.7415294846857	18.941873925557626	44.31659658975668
2	26.633784622784606	21.851041967183672	27.686202701956457	23.828970708075264
3	28.864134745847124	22.28936026585014	24.218621379796897	24.62788360850584
4	23.70180524382068	23.714552755862435	27.505724768312678	25.077917232004204
5	24.62690303065648	27.380262412954366	24.933875506868432	23.05895904952073
6	25.310365791664363	24.21392492799204	21.626747686481394	28.848961593862203
7	29.874233149414774	21.625405843108574	21.646152806026734	26.854208201449914
8	27.099713929314788	20.38430394197456	26.93595742847072	25.580024700239935
9	27.86864179129897	27.03566671294306	23.170796533708913	21.92489496204906
10	36.13790640436037	19.449658423447577	22.78310701768601	21.629328154506037
11	26.869329744074342	20.029386369864675	25.91909819867849	27.182185687382493
12	28.237751937544413	21.292215811766212	22.230009501283266	28.24002274940611
13	27.180482578486227	23.12202568804309	23.851317561168706	25.84617417230198
14	21.262695257564253	22.070072093115673	21.021473106620295	35.64575954269978
15	21.80805136989306	29.81173421385784	25.60479719327654	22.775417222972568
16	21.798090763317923	31.468910779285856	20.034856962076926	26.698141495319287
17	22.619040860678982	34.87884444577482	18.20370524242723	24.298409451118967
18	23.41212190337386	22.288224859919293	19.98020264931491	34.319450587391934
19	27.595267008767916	20.49510923895289	21.719541316647685	30.190082435631517
20	26.475137448629333	19.04545391206694	22.74274849778054	31.73666014152319
21	25.7036291186205	27.050323771323054	19.707860053993713	27.53818705606273
22	24.951887173680465	20.30627058890925	24.90796760790098	29.833874629509303
23	31.369356322894998	20.037850304985515	26.1205295326824	22.472263839437087
24	27.33933619008347	21.380416208848633	25.284612720778394	25.995634880289508
25	25.081568487868537	27.468624102226258	20.493906951040174	26.955900458865024
26	25.71043221715823	30.95552837550242	21.920759574442364	21.413279832896986
27	24.815790239886926	22.09447780986387	25.066714663462175	28.023017286787027
28	23.192989177384078	21.635413959856596	22.437385647001197	32.73421121575813
29	25.724036006986427	21.595109498857987	26.16127905414483	26.51957544001075
30	24.203978023431123	24.627012883974857	24.966481288592583	26.202527804001434
31	23.877322468075022	32.04712452693372	22.168850875530946	21.90670212946031
32	27.3323968476417	22.944944875546994	22.16004661519847	27.562611661612834
33	22.495505556917227	21.223919198730353	28.11275534591962	28.167819898432793
34	24.091299270448037	25.969436223821607	28.017018225116182	21.92224628061418
35	21.52868695298154	21.721380478490346	22.10778111321899	34.642151455309126
36	22.76146787950148	22.2097255921531	27.96643718997109	27.06236933837433
37	23.435094069384608	29.00489851763215	21.611763306190525	25.948244106792718
38	28.396214883542864	25.8701708745298	20.633981637157426	25.099632604769912
39	26.650838118708297	28.96246483621231	22.58270755353033	21.803989491549068
40	26.406691606759626	23.32369738832663	23.387845809243473	26.881765195670276
41	26.696401691359434	27.531789880115525	22.663897023205028	23.10791140532001
42	21.68855904374097	33.19764039979461	22.000585123532712	23.113215432931707
43	25.554224284988113	22.40027882313002	23.60222117837746	28.443275713504413
44	28.763337725279797	21.07629643996967	20.413795535536767	29.746570299213765
45	21.772953103577063	21.53436761330968	29.332562619477027	27.36011666363623
46	21.244903272206937	24.439383354466298	31.939806325904645	22.37590704742212
47	22.35997337652654	34.893470599589804	21.841835651051735	20.90472037283192
48	30.32984993933363	27.332806121952608	20.107670647622196	22.229673291091565
49	19.93515940887755	27.120187162991076	23.740403582596382	29.204249845534996
50	20.402494600488993	27.84827506086001	30.183926619457253	21.565303719193743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1533.0
1	1279.0
2	965.5
3	1190.5
4	1494.5
5	1489.0
6	1570.0
7	1776.0
8	2225.0
9	2634.0
10	2759.5
11	2557.5
12	2134.0
13	2339.5
14	2860.5
15	3249.5
16	3577.5
17	4563.5
18	5227.5
19	5445.0
20	5974.5
21	7507.0
22	9560.5
23	12593.0
24	15832.0
25	16653.0
26	17280.5
27	20162.0
28	25655.5
29	26197.0
30	27778.0
31	38041.0
32	46096.0
33	48631.0
34	55219.0
35	60300.0
36	52042.5
37	46803.5
38	52236.0
39	58181.0
40	64667.0
41	75953.0
42	88012.0
43	92218.5
44	87400.5
45	87060.0
46	96927.5
47	102765.5
48	110304.5
49	123297.5
50	156764.0
51	181084.0
52	139692.5
53	100095.0
54	103862.5
55	111468.5
56	116745.5
57	113486.0
58	107802.5
59	114299.5
60	118475.5
61	111039.0
62	100362.5
63	94332.0
64	86300.5
65	79372.5
66	110320.0
67	141830.5
68	104253.5
69	64893.0
70	62708.5
71	60220.0
72	52993.0
73	44531.5
74	35876.0
75	28338.0
76	23714.0
77	18418.5
78	14715.0
79	12309.5
80	8696.5
81	5789.5
82	4623.5
83	3924.5
84	3359.0
85	2428.5
86	1744.0
87	1331.0
88	725.5
89	460.5
90	476.0
91	552.0
92	514.5
93	368.5
94	307.0
95	145.5
96	11.5
97	10.0
98	172.5
99	336.0
100	336.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15935.0
25	19159.0
26	13524.0
27	14884.0
28	13381.0
29	15512.0
30	13835.0
31	13970.0
32	14095.0
33	12787.0
34	14674.0
35	12735.0
36	14858.0
37	13912.0
38	13852.0
39	15100.0
40	14709.0
41	15851.0
42	16461.0
43	13722.0
44	16749.0
45	26165.0
46	52754.0
47	109991.0
48	430699.0
49	629581.0
50	378738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.412398086990837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.65881650023736	5.691728984946609
2	13.504291178347536	2.2720694654979607
3	5.477386688089736	1.3823387188978555
4	2.94160638601159	0.9898385573745573
5	1.7779206781522683	0.7478288255854796
6	1.1324157235626109	0.5715799119945871
7	0.8281147476261735	0.4876501643117526
8	0.6301456974421361	0.4240829167750186
9	0.4955451130520135	0.3751850484950775
>10	3.4527703980655593	5.8779523313124775
>50	0.5630816422777857	3.3290020409300696
>100	0.8112297000824682	17.24869013378937
>500	0.6133239650925609	37.38784842555843
>1k	0.10783718067561511	12.278742547771362
>5k	0.003063556269193611	1.6282657924779502
>10k+	0.002450845015354889	9.30719613428145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	80335	4.146037975199638	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	67813	3.4997855631071517	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	20185	1.0417349415498187	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	12236	0.6314921349915077	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	8065	0.4162294923754912	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	6392	0.329887032270817	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	6138	0.3167782547056125	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	5660	0.2921089803899913	No Hit
AATTCGTGGAGAAAGAAATGGCTCC	5335	0.2753359382297886	No Hit
CTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCT	4541	0.23435810599840112	No Hit
CATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTG	4255	0.21959782889742277	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	4151	0.21423045540615795	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	3296	0.17010445218470163	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	3185	0.16437581316998628	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2891	0.14920266118506445	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	2855	0.14734472420731892	No Hit
CTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAAGGTCGA	2822	0.14564161531105219	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	2649	0.13671319594577508	No Hit
AAGGTATTGGAAAAACCATTTCATAACTTTGTCAAAGTTAAATTATAGGC	2573	0.1327908845483123	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	2518	0.12995236972120106	No Hit
CCTTTCGTTGCCTGATCGCCGCCATCATGGGTCGCATGCATGCTCCCGG	2485	0.12824926082493435	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG	2429	0.12535913663733017	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	2306	0.11901118529669963	No Hit
AATTCGTGGAGAAAGAAATGGCTC	2280	0.11766934192388341	No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCT	2224	0.11477921773627925	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	2207	0.11390185860789942	No Hit
CTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTTCAGTCGC	2110	0.1088957506400851	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2077	0.10719264174381836	No Hit
CTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGCTATTC	2076	0.10714103238332544	No Hit
CGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGACCGAGG	1957	0.10099951848466661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03153331926118104	0.0	0.0	0.0	0.0
2	0.06193123259151759	0.0	0.0	0.0	0.0
3	0.07963324324059304	0.0	0.0	0.0	0.0
4	0.10342515842783437	0.0	0.0	0.0	0.0
5	0.12762994849901915	0.0	0.0	0.0	0.0
6	0.17170434235998253	0.0	0.0	0.0	0.0
7	0.21330148691728515	0.0	0.0	0.0	0.0
8	0.28039365555809587	0.0	0.0	0.0	0.0
9	0.328390360816522	0.0	0.0	0.0	0.0
10	0.37406464485276625	0.0	0.0	0.0	0.0
11	0.42118399098281256	0.0	0.0	0.0	0.0
12	0.47083219577701246	0.0	0.0	0.0	0.0
13	0.5185708542329739	0.0	0.0	0.0	0.0
14	0.570335042807384	0.0	0.0	0.0	0.0
15	0.6246280900459478	0.0	0.0	0.0	0.0
16	0.6794888402499338	0.0	0.0	0.0	0.0
17	0.7329045283601178	0.0	0.0	0.0	0.0
18	0.7855460760629077	0.0	0.0	0.0	0.0
19	0.8429872942915403	0.0	0.0	0.0	0.0
20	0.8998092001942576	0.0	0.0	0.0	0.0
21	0.9503347641168374	0.0	0.0	0.0	0.0
22	0.9936350175704067	0.0	0.0	0.0	0.0
23	1.037761020791863	0.0	0.0	0.0	0.0
24	1.079771040233109	0.0	0.0	0.0	0.0
25	1.1220907158373128	0.0	0.0	0.0	0.0
26	1.1713260457475694	0.0	0.0	0.0	0.0
27	1.215039174085082	0.0	0.0	0.0	0.0
28	1.2614359891682274	0.0	0.0	0.0	0.0
29	1.308400507216795	0.0	0.0	0.0	0.0
30	1.35567468142832	0.0	0.0	0.0	0.0
31	1.4024843713954087	0.0	0.0	0.0	0.0
32	1.448210264792146	0.0	0.0	0.0	0.0
33	1.4949167360382487	0.0	0.0	0.0	0.0
34	1.5476098931015316	0.0	0.0	0.0	0.0
35	1.594626020510592	0.0	0.0	0.0	0.0
36	1.6423646789665536	0.0	0.0	0.0	0.0
37	1.6948513985878646	0.0	0.0	0.0	0.0
38	1.735209918493337	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTA	80	0.0	205.26949	44
CGAATAT	115	0.0	196.34474	44
ACCACGA	115	0.0	196.34474	44
CTTGCGT	60	0.0	188.16371	44
CCCATAC	860	0.0	173.04695	44
CTATCAT	2505	0.0	169.6239	44
CTTATTA	80	0.0	166.78146	44
TATACTT	130	0.0	165.79459	44
AATCGCA	720	0.0	162.50502	44
AGGGCGA	65	0.0	157.89961	44
ACTTCTA	1105	0.0	143.96729	44
GTAACAG	80	0.0	141.12277	44
ATTAGTC	495	0.0	140.9932	44
ACAAATC	110	0.0	139.95648	44
ACCTGAC	140	0.0	139.29001	44
CATAATA	105	0.0	136.84633	44
ATTACCA	215	0.0	124.11643	44
TCAACCG	240	0.0	124.01699	44
AGACGAT	245	0.0	121.48602	44
CTGACGA	295	0.0	114.81176	44
>>END_MODULE