FastQCFastQC Report
Tue 24 May 2016
ERR841228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590874
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC650462.5105813713827843No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT602992.3273613460168265No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA461541.7814065832610926No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA186300.7190623704587719No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC132770.5124525546205644No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG129930.501491002650071No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA101030.3899456322461069No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC100240.3868964681416387No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC67830.2618035458304804No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG65380.25234727740523083No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA57520.22201002441647105No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC55110.21270814404714394No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA47610.1837603835616861No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG47190.1821393089745005No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT46570.17974629410770265No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG40460.1561635185655497No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC38670.14925465306302044No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC37920.14635987701447467No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG37440.14450722034340535No Hit
ACGGTTCGTTTTTCCTTTAGTCAGGAAGGACGTTGGTGTTGAGGTTAGC33780.13038071322650194No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA32400.1250543252971777No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA30090.11613841506765671No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG28310.10926814657910805No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC27900.10768566900590303No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC27380.1056786242789113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACG800.0123.0524544
ATAAGCG600.0123.0524544
CAATAAC259.8825694E-8123.0524544
TAGCTCG650.0113.5868844
AACGCGT650.0104.12131544
ATGCGGC650.0104.12131544
CTATCAT80700.099.7990544
AATCGCA5150.099.15877544
CTAAATT3100.095.2664244
CTCTATT850.094.0989444
GTTCGTG1650.089.4926944
TAAGCCA28000.087.0156644
ACCACTC8700.085.5709644
ACTTCTA14850.080.3776944
CCCTTGT3450.080.251644
ACTATAG1850.079.8178144
AGTATTG1100.078.3061144
CCTCGAA1350.077.4774744
TAATTCC4200.076.1753344
CGACGGG3250.075.7245944
Produced by FastQC (version 0.11.5)