##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841228.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2590874
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25032981148446	38.0	35.0	39.0	31.0	39.0
2	36.967661877806485	39.0	37.0	39.0	33.0	39.0
3	37.024511805668666	39.0	36.0	39.0	33.0	39.0
4	37.46724773184647	39.0	37.0	40.0	33.0	41.0
5	37.98795966148875	39.0	37.0	40.0	33.0	41.0
6	37.745849856071736	39.0	36.0	40.0	33.0	41.0
7	37.77634728666852	39.0	37.0	40.0	33.0	41.0
8	37.49957736269691	39.0	36.0	40.0	33.0	41.0
9	37.48138813388841	39.0	36.0	40.0	33.0	41.0
10	37.47732193846555	39.0	36.0	40.0	33.0	41.0
11	37.07815393569892	39.0	35.0	40.0	31.0	41.0
12	37.59148727417852	39.0	36.0	40.0	33.0	41.0
13	37.74950615120612	39.0	36.0	40.0	33.0	41.0
14	37.760361175418026	39.0	36.0	41.0	33.0	41.0
15	37.656118746029335	39.0	36.0	41.0	33.0	41.0
16	37.78096696327185	39.0	36.0	41.0	33.0	41.0
17	37.768883782075086	39.0	36.0	41.0	33.0	41.0
18	37.70023860674043	39.0	36.0	41.0	33.0	41.0
19	37.70190561177425	39.0	36.0	41.0	33.0	41.0
20	37.574723047126184	39.0	35.0	41.0	33.0	41.0
21	37.50558614583341	39.0	35.0	41.0	33.0	41.0
22	37.40921133177453	39.0	35.0	41.0	32.0	41.0
23	37.33140592711185	39.0	35.0	41.0	32.0	41.0
24	37.07174412958716	39.0	35.0	40.0	31.0	41.0
25	37.082384991168965	39.0	35.0	40.0	31.0	41.0
26	37.066559406669086	39.0	35.0	40.0	31.0	41.0
27	36.96540210123862	38.0	35.0	40.0	31.0	41.0
28	37.00055751800652	39.0	35.0	40.0	31.0	41.0
29	36.93285746484916	38.0	35.0	40.0	31.0	41.0
30	36.848330981112234	38.0	35.0	40.0	31.0	41.0
31	36.67004201834282	38.0	35.0	40.0	31.0	41.0
32	36.630185309461574	38.0	35.0	40.0	31.0	41.0
33	36.48365076277196	38.0	34.0	40.0	31.0	41.0
34	36.469296598404064	38.0	34.0	40.0	31.0	41.0
35	36.472577855996406	38.0	34.0	40.0	31.0	41.0
36	36.41346578831057	38.0	34.0	40.0	31.0	41.0
37	36.2429309075157	37.0	34.0	40.0	31.0	41.0
38	36.09327262712457	37.0	34.0	40.0	30.0	41.0
39	36.07039293236488	37.0	34.0	40.0	31.0	41.0
40	36.07061468217159	37.0	34.0	40.0	31.0	41.0
41	35.893823710124785	36.0	34.0	40.0	30.0	41.0
42	35.69538743780417	36.0	34.0	40.0	30.0	41.0
43	35.67788967224693	36.0	34.0	40.0	30.0	41.0
44	35.673434635925375	36.0	34.0	40.0	30.0	41.0
45	35.373218131833674	36.0	33.0	39.0	30.0	41.0
46	35.25091337042856	35.0	33.0	39.0	29.0	41.0
47	35.18854145393025	35.0	33.0	39.0	29.0	41.0
48	35.23027550777213	35.0	33.0	39.0	29.0	41.0
49	35.583835508837964	36.0	34.0	39.0	29.0	41.0
50	35.885927341041615	37.0	34.0	39.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	61.0
14	437.0
15	3020.0
16	1015.0
17	3.0
18	0.0
19	1.0
20	2.0
21	12.0
22	46.0
23	150.0
24	468.0
25	1144.0
26	2591.0
27	5870.0
28	12115.0
29	23604.0
30	44167.0
31	75506.0
32	116367.0
33	172235.0
34	234584.0
35	238318.0
36	284335.0
37	322987.0
38	362434.0
39	416052.0
40	273350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.332162814556014	21.230557719132616	36.43727946631137
2	26.564201887085208	21.81310245114197	28.544537480402365	23.078158181370455
3	28.441522050088118	21.01256178417013	24.868094704721262	25.67782146102049
4	24.220166631028757	21.19331160064133	28.58074147951618	26.005780288813735
5	22.410468436519874	25.285598604949527	24.634158202984786	27.669774755545813
6	22.912499797365676	24.015023501721814	23.47925835065696	29.59321835025555
7	27.02142211469952	22.365657303288387	21.55504281566761	29.057877766344486
8	24.552564115429774	23.418313665581575	25.84270790474566	26.186414314242995
9	27.335563211487706	27.02088175650379	23.98634592033422	21.657209111674284
10	34.822226013306704	19.769737933994474	22.034417729306792	23.37361832339203
11	26.090963898668946	20.33136308442634	24.990640224109704	28.58703279279502
12	28.137261788878966	22.06031632568778	23.539701274550595	26.26272061088266
13	27.754572395261214	22.730862249572926	22.961942572274836	26.55262278289102
14	22.5599160746528	22.14075250282337	20.528478034825316	34.770853387698516
15	24.995966612039027	25.905389455450166	25.097090788668226	24.001553143842578
16	20.986585993761178	28.636514164718164	21.665468872666136	28.711430968854522
17	22.869464126777295	34.641784972947356	18.756952287143257	23.731798613132092
18	23.52592214055952	23.84102816269722	21.41169350574362	31.22135619099964
19	29.044021438325444	22.231455485677806	21.314853597666268	27.40966947833048
20	24.87812992835622	19.811577097149456	24.66731303799413	30.642979936500193
21	23.77082019426649	26.790882150193333	22.5308525231254	26.907445132414775
22	26.70469501797463	22.024807072825617	25.288570575026032	25.981927334173722
23	32.08249416992104	20.78043934209074	25.41482140775661	21.722245080231612
24	26.88579220757165	21.084468021216008	27.473431745426446	24.556308025785892
25	24.96830208569746	27.0796537345924	20.296630970098267	27.655413209611872
26	25.79203986932593	28.41293791019591	24.42321917337859	21.371803047099565
27	23.793830907862738	25.48098281647066	23.007668008416356	27.717518267250245
28	22.37492287201647	21.006248115481306	23.91476683886092	32.704062173641304
29	24.090267437120403	23.04736467968793	26.770565828675245	26.091802054516418
30	22.73092055678457	25.84053243216406	24.698565714871734	26.729981296179634
31	25.185373229604014	29.263276198410047	23.356718847623636	22.1946317243623
32	26.946935579267304	24.039669945259092	22.578612804511334	26.434781670962266
33	22.944448279396475	21.779445633279572	26.65792297603434	28.618183111289614
34	24.46612962439393	26.27250745852973	27.158416217607616	22.102946699468724
35	22.684191535027328	20.601436189315557	23.80452302617582	32.9098492494813
36	21.824231134164354	22.84521916021265	28.357351822508726	26.97319788311427
37	22.199306909248428	31.014814814814816	21.29772579597141	25.488152479965347
38	26.92348045044415	25.80646289431349	21.534237952017907	25.73581870322445
39	26.346117934892256	27.541730329211696	24.624326604417536	21.487825131478512
40	25.084666812435348	25.83000195496282	24.584793061173414	24.500538171428417
41	26.09577197892709	28.425774895981608	21.306094306709632	24.172358818381667
42	22.068176786757686	28.817172399025914	23.005922893507684	26.108727920708713
43	26.571588195151328	22.97470527064045	21.884654640850222	28.569051893357994
44	24.53080421188773	24.167445281915853	20.25591697405949	31.04583353213693
45	22.385056691231465	23.56301558224932	28.434913898407892	25.617013828111325
46	21.119324684553217	26.86168730749685	29.378165124418885	22.640822883531044
47	23.519732272587916	29.026841466749264	26.217282865065446	21.236143395597374
48	26.225428233614213	26.332186138222795	20.364703613690445	27.077682014472547
49	21.274243426402123	28.02541056262297	22.530694573804855	28.169651437170053
50	20.65944304131018	27.33327765236489	27.472205589820263	24.535073716504662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2020.0
1	1612.5
2	1190.5
3	1372.5
4	1576.5
5	1561.0
6	1607.5
7	1686.5
8	1805.5
9	2175.0
10	2503.5
11	2480.5
12	2404.0
13	3835.5
14	5849.0
15	7018.0
16	7167.5
17	6485.0
18	6666.0
19	7802.0
20	8555.0
21	11428.0
22	15187.0
23	19484.0
24	24019.5
25	26833.5
26	28986.0
27	28158.5
28	29512.0
29	37593.0
30	44082.5
31	52204.5
32	59979.0
33	54284.0
34	58309.0
35	76305.5
36	79380.5
37	75219.0
38	75079.5
39	81662.5
40	89999.0
41	95349.5
42	110434.5
43	131855.0
44	139235.5
45	132818.0
46	131426.5
47	131542.0
48	142100.0
49	179554.0
50	235396.5
51	238803.0
52	182515.5
53	150305.0
54	147237.0
55	149652.5
56	150645.0
57	150734.5
58	149535.0
59	138802.5
60	132883.0
61	137463.5
62	137437.5
63	130102.5
64	119952.5
65	113764.5
66	134505.5
67	152421.0
68	122102.5
69	93980.0
70	85114.0
71	75855.5
72	65593.0
73	52609.0
74	41963.0
75	33520.5
76	28803.5
77	25735.0
78	24005.5
79	20575.0
80	15652.0
81	11913.5
82	9075.5
83	6526.5
84	4482.0
85	3556.5
86	3048.5
87	2191.5
88	1364.5
89	1167.0
90	950.0
91	794.0
92	870.0
93	833.0
94	729.0
95	436.5
96	163.5
97	55.0
98	498.0
99	940.5
100	940.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22093.0
25	20456.0
26	22767.0
27	21603.0
28	23858.0
29	22845.0
30	22839.0
31	23419.0
32	20699.0
33	20525.0
34	19662.0
35	21971.0
36	19637.0
37	20980.0
38	22321.0
39	19632.0
40	20720.0
41	20519.0
42	22411.0
43	23170.0
44	22085.0
45	37021.0
46	48002.0
47	117356.0
48	378552.0
49	705650.0
50	850081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.73765557453302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.67461681571078	5.159052204839376
2	13.289746113251317	2.056629561946552
3	5.546508243710161	1.2875091129341183
4	2.9470583472218257	0.9121328979542009
5	1.8109475709703564	0.7006244283852904
6	1.1894089354706419	0.5521942007966278
7	0.8775762158279748	0.4753267748935246
8	0.6592095975359442	0.40805894537277343
9	0.5192201295710561	0.3615791876986715
>10	4.078752447923455	6.4636573852349875
>50	0.6447113484113757	3.5407941549882436
>100	0.9795228059996954	18.794629131598004
>500	0.6721140718685601	37.76354925436142
>1k	0.10462858049844154	11.4581002499934
>5k	0.001992925342827458	0.9477482253507138
>10k+	0.003985850685654916	9.118414283652088
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	65046	2.5105813713827843	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	60299	2.3273613460168265	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	46154	1.7814065832610926	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	18630	0.7190623704587719	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	13277	0.5124525546205644	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAAG	12993	0.501491002650071	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10103	0.3899456322461069	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	10024	0.3868964681416387	No Hit
ATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC	6783	0.2618035458304804	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG	6538	0.25234727740523083	No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGGA	5752	0.22201002441647105	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	5511	0.21270814404714394	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	4761	0.1837603835616861	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	4719	0.1821393089745005	No Hit
CTTTTACCTCGTTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGT	4657	0.17974629410770265	No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGG	4046	0.1561635185655497	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCC	3867	0.14925465306302044	No Hit
ATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTC	3792	0.14635987701447467	No Hit
CTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTTTGCGTCG	3744	0.14450722034340535	No Hit
ACGGTTCGTTTTTCCTTTAGTCAGGAAGGACGTTGGTGTTGAGGTTAGC	3378	0.13038071322650194	No Hit
CTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGA	3240	0.1250543252971777	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCCA	3009	0.11613841506765671	No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGG	2831	0.10926814657910805	No Hit
TGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC	2790	0.10768566900590303	No Hit
AATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	2738	0.1056786242789113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01887393983651849	0.0	0.0	0.0	0.0
2	0.03242149174371274	0.0	0.0	0.0	0.0
3	0.04357602878410915	0.0	0.0	0.0	0.0
4	0.05913062541829514	0.0	0.0	0.0	0.0
5	0.07653787872355043	0.0	0.0	0.0	0.0
6	0.1016259378109472	0.0	0.0	0.0	0.0
7	0.12628942974455723	0.0	0.0	0.0	0.0
8	0.16129692142497087	0.0	0.0	0.0	0.0
9	0.1895499356587777	0.0	0.0	0.0	0.0
10	0.21212918883743478	0.0	0.0	0.0	0.0
11	0.236676889729103	0.0	0.0	0.0	0.0
12	0.2670527397318434	0.0	0.0	0.0	0.0
13	0.29549873903555324	0.0	0.0	0.0	0.0
14	0.3250640517447008	0.0	0.0	0.0	0.0
15	0.35852766286589005	0.0	0.0	0.0	0.0
16	0.388054378561057	0.0	0.0	0.0	0.0
17	0.42672858656962864	0.0	0.0	0.0	0.0
18	0.46135010811023613	0.0	0.0	0.0	0.0
19	0.49774709229395175	0.0	0.0	0.0	0.0
20	0.5306703452194125	0.0	0.0	0.0	0.0
21	0.56340061307497	0.0	0.0	0.0	0.0
22	0.594316821273439	0.0	0.0	0.0	0.0
23	0.6249628503740436	0.0	0.0	0.0	0.0
24	0.6550299242649391	0.0	0.0	0.0	0.0
25	0.686949654826904	0.0	0.0	0.0	0.0
26	0.719834310738384	0.0	0.0	0.0	0.0
27	0.7517540413003488	0.0	0.0	0.0	0.0
28	0.7847544882537707	0.0	0.0	0.0	0.0
29	0.8224637709128271	0.0	0.0	0.0	0.0
30	0.8642643370538282	0.0	0.0	0.0	0.0
31	0.9002753510977377	0.0	0.0	0.0	0.0
32	0.935514424862035	0.0	0.0	0.0	0.0
33	0.9723359761995373	0.0	0.0	0.0	0.0
34	1.018536601934328	0.0	0.0	0.0	0.0
35	1.0536598846566834	0.0	0.0	0.0	0.0
36	1.0949586896159365	0.0	0.0	0.0	0.0
37	1.1342890468621785	0.0	0.0	0.0	0.0
38	1.1653982401305505	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATACG	80	0.0	123.05245	44
ATAAGCG	60	0.0	123.05245	44
CAATAAC	25	9.8825694E-8	123.05245	44
TAGCTCG	65	0.0	113.58688	44
AACGCGT	65	0.0	104.121315	44
ATGCGGC	65	0.0	104.121315	44
CTATCAT	8070	0.0	99.79905	44
AATCGCA	515	0.0	99.158775	44
CTAAATT	310	0.0	95.26642	44
CTCTATT	85	0.0	94.09894	44
GTTCGTG	165	0.0	89.49269	44
TAAGCCA	2800	0.0	87.01566	44
ACCACTC	870	0.0	85.57096	44
ACTTCTA	1485	0.0	80.37769	44
CCCTTGT	345	0.0	80.2516	44
ACTATAG	185	0.0	79.81781	44
AGTATTG	110	0.0	78.30611	44
CCTCGAA	135	0.0	77.47747	44
TAATTCC	420	0.0	76.17533	44
CGACGGG	325	0.0	75.72459	44
>>END_MODULE