##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841227.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7756089
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.06334455419478	39.0	37.0	39.0	33.0	39.0
2	37.5515114640897	39.0	37.0	39.0	35.0	39.0
3	37.65793778281812	39.0	37.0	39.0	35.0	39.0
4	37.97402402164287	39.0	37.0	40.0	35.0	41.0
5	38.641668629640535	40.0	38.0	41.0	35.0	41.0
6	38.58011969692457	40.0	38.0	41.0	34.0	41.0
7	38.60855052075859	40.0	38.0	41.0	35.0	41.0
8	38.549104967722776	40.0	38.0	41.0	34.0	41.0
9	38.496777048329385	40.0	38.0	41.0	34.0	41.0
10	38.51235345545932	40.0	38.0	41.0	34.0	41.0
11	38.30001718649696	40.0	38.0	41.0	34.0	41.0
12	38.52003361487987	40.0	38.0	41.0	34.0	41.0
13	38.637488945781826	40.0	38.0	41.0	35.0	41.0
14	38.62000191075683	40.0	38.0	41.0	34.0	41.0
15	38.59927251995175	40.0	38.0	41.0	34.0	41.0
16	38.62736593146365	40.0	38.0	41.0	34.0	41.0
17	38.579799948143965	40.0	38.0	41.0	34.0	41.0
18	38.63897784050699	40.0	38.0	41.0	34.0	41.0
19	38.62809980648752	40.0	38.0	41.0	34.0	41.0
20	38.61402131924995	40.0	38.0	41.0	34.0	41.0
21	38.570712636226844	40.0	38.0	41.0	34.0	41.0
22	38.52289059602075	40.0	38.0	41.0	34.0	41.0
23	38.493199472053504	40.0	38.0	41.0	34.0	41.0
24	38.3458989446872	40.0	38.0	41.0	34.0	41.0
25	38.28143076102763	40.0	37.0	41.0	34.0	41.0
26	38.276683903935975	40.0	37.0	41.0	34.0	41.0
27	38.277219808152225	40.0	37.0	41.0	34.0	41.0
28	38.289393188950584	40.0	37.0	41.0	34.0	41.0
29	38.23824661888855	40.0	37.0	41.0	34.0	41.0
30	38.185976863591065	40.0	37.0	41.0	33.0	41.0
31	38.07860507498928	40.0	37.0	41.0	33.0	41.0
32	38.02756738807504	40.0	37.0	41.0	33.0	41.0
33	37.99761050132651	40.0	37.0	41.0	33.0	41.0
34	37.956663504676314	40.0	36.0	41.0	33.0	41.0
35	37.950056129269925	40.0	36.0	41.0	33.0	41.0
36	37.9180839833903	40.0	36.0	41.0	33.0	41.0
37	37.8505780242883	40.0	36.0	41.0	33.0	41.0
38	37.75995727898505	40.0	36.0	41.0	33.0	41.0
39	37.70290536623973	39.0	36.0	41.0	33.0	41.0
40	37.68470657350521	39.0	36.0	41.0	33.0	41.0
41	37.591031814340106	39.0	35.0	41.0	33.0	41.0
42	37.449065875394155	39.0	35.0	41.0	32.0	41.0
43	37.41423323863397	39.0	35.0	41.0	32.0	41.0
44	37.35143164155277	39.0	35.0	41.0	32.0	41.0
45	37.230952376125245	39.0	35.0	41.0	32.0	41.0
46	37.079076682563155	39.0	35.0	40.0	31.0	41.0
47	37.02425188692505	39.0	35.0	40.0	31.0	41.0
48	37.02337099546226	39.0	35.0	40.0	31.0	41.0
49	37.12246197616141	39.0	35.0	40.0	31.0	41.0
50	37.092894344538756	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	9.0
14	489.0
15	7911.0
16	11080.0
17	354.0
18	0.0
19	0.0
20	2.0
21	17.0
22	56.0
23	158.0
24	459.0
25	1146.0
26	2746.0
27	6385.0
28	14012.0
29	29763.0
30	57794.0
31	105047.0
32	174539.0
33	273575.0
34	427498.0
35	470925.0
36	635139.0
37	801564.0
38	1065666.0
39	1669291.0
40	2000464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	37.52770242837595	28.77653930995377	33.695758261670285
2	23.99884787294215	26.66909830457077	28.925712430581957	20.406341391905123
3	26.14208011279912	22.754651216611876	28.28186989602621	22.821398774562798
4	23.64803962409405	25.19983976460301	28.149109686595914	23.003010924707027
5	22.33923566374754	26.65478696801958	26.135440168363207	24.870537199869677
6	21.63696161815575	27.35388931199732	27.045795890170936	23.963353179675995
7	24.382610875145964	24.98438323747961	25.88423108605381	24.748774801320614
8	22.19233172801395	26.50545913023948	27.982930572354185	23.31927856939238
9	23.637531750860518	28.262955724205845	27.168112691847657	20.93139983308598
10	27.47494259026682	26.078156142870462	24.85959096137241	21.587310305490305
11	21.990000372610474	26.265712010267027	27.678769544805377	24.06551807231712
12	24.75505373906875	26.130205571390423	25.45369193159078	23.66104875795004
13	23.418748804971166	27.191952026336985	26.269438115008736	23.119861053683113
14	21.26698133556745	26.50451793423206	25.622011299767188	26.6064894304333
15	22.15599898350831	29.253648327140137	27.65725096759462	20.933101721756934
16	22.17377856288137	29.258676634577043	24.95440678929806	23.613138013243532
17	22.49824621661768	31.491670608730765	24.244319527535076	21.76576364711648
18	23.42858623721311	26.3000463248939	24.795989318843557	25.475378119049434
19	24.06804511913156	26.04382182824359	25.492177823127093	24.395955229497755
20	23.244060247374676	25.739093504471132	26.160065981708048	24.856780266446144
21	22.54089657815943	28.343640718924192	25.397374888297442	23.71808781461894
22	23.397642806832156	26.039799182294065	27.029705306372836	23.532852704500943
23	25.02770404001295	26.790641520487966	26.601190367980564	21.580464071518517
24	23.534670631035823	26.06111146996895	27.733397592523758	22.67082030647147
25	22.459971108700692	28.154678773891938	25.292176649436378	24.09317346797099
26	22.72922287149516	29.93333396739176	26.09146101784879	21.245982143264285
27	22.65898294737374	27.668300717972937	25.976768992197734	23.69594734245559
28	23.68899358802544	25.836703892084607	25.190890193127295	25.28341232676266
29	22.338751516026072	27.02108838844942	26.970675318421637	23.669484777102873
30	22.23440370596855	27.59446016927039	27.005234009976686	23.165902114784366
31	23.22014302951446	29.96883080395748	25.93809371963801	20.87293244689005
32	24.233295194861363	26.722733487811823	26.20043201520848	22.84353930211833
33	22.07635547277369	25.577607230591408	27.796161419952405	24.549875876682492
34	22.303887755989297	28.044602647960055	28.111545686962415	21.539963909088232
35	20.906823775906105	26.929983882433106	25.57545112690723	26.587741214753557
36	21.844380727297295	26.641260508681363	28.99877363601095	22.515585128010393
37	21.420070941129445	29.639629920054478	25.92112480670175	23.019174332114332
38	23.114698969399853	28.54071957586035	26.307496255052442	22.03708519968735
39	23.36428061844408	27.94897947809027	26.55054119430603	22.136198709159615
40	22.992265928617606	28.09302243451083	26.902591790507525	22.01211984636404
41	23.4301516173623	28.262860760011193	25.753663551704907	22.553324070921597
42	21.34990159456108	29.971021006259274	26.011963003016497	22.667114396163146
43	22.822617995392562	26.993917161835373	25.91385028531914	24.269614557452922
44	23.580334560561468	26.992923022776754	24.913636839387237	24.51310557727454
45	21.25724179417795	27.241209128737182	28.679430973026626	22.82211810405824
46	20.670093268344246	28.608528443834075	30.34297879903487	20.378399488786812
47	22.105800645115757	30.140747677185924	25.67817809061629	22.075273587082027
48	23.565563933637275	29.18259533274159	25.252761673726088	21.999079059895045
49	20.513952218477517	28.774976783981472	26.274828966635017	24.436242030906
50	19.692490240611022	29.67990685216484	28.526410589869833	22.10119231735431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6159.0
1	4836.5
2	2913.0
3	2419.5
4	2697.5
5	2800.5
6	2685.5
7	2691.0
8	2830.0
9	3364.0
10	3901.5
11	4261.5
12	4829.0
13	7021.5
14	9678.5
15	11152.5
16	12423.0
17	18853.5
18	25395.0
19	30392.0
20	35520.5
21	51161.5
22	67529.5
23	79639.5
24	91171.5
25	105001.5
26	125137.5
27	152590.0
28	178951.5
29	197003.0
30	219853.0
31	263681.5
32	307265.0
33	327895.5
34	358338.5
35	392897.0
36	410669.0
37	425087.5
38	439829.5
39	457258.0
40	486282.0
41	523672.5
42	546811.5
43	536096.0
44	522127.0
45	525168.5
46	534331.0
47	518525.5
48	506108.0
49	523189.5
50	565494.0
51	564238.5
52	479499.0
53	396748.5
54	355555.5
55	327944.0
56	292596.0
57	273385.5
58	267730.5
59	254306.5
60	244145.5
61	228193.5
62	202297.0
63	189326.0
64	179488.0
65	168734.0
66	200779.0
67	230843.0
68	174376.5
69	119689.0
70	109010.0
71	97184.0
72	81854.5
73	71333.0
74	63075.5
75	52782.5
76	47489.0
77	40874.0
78	35393.0
79	31763.5
80	24955.0
81	17281.5
82	13320.0
83	10503.5
84	8247.5
85	6328.0
86	4195.0
87	2679.0
88	1453.5
89	1028.5
90	1130.0
91	1063.5
92	823.0
93	717.0
94	394.5
95	100.5
96	95.5
97	95.5
98	243.5
99	392.0
100	392.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42348.0
25	38318.0
26	35720.0
27	36117.0
28	36104.0
29	36927.0
30	41751.0
31	41381.0
32	36245.0
33	39239.0
34	37085.0
35	37673.0
36	39614.0
37	40510.0
38	40337.0
39	41151.0
40	42384.0
41	44286.0
42	46740.0
43	46647.0
44	50110.0
45	63638.0
46	96928.0
47	203694.0
48	790802.0
49	2239362.0
50	3510978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.32688106055692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38964144286834	5.69472410068899
2	14.31765088639995	2.3844275199525944
3	5.632595803477609	1.4070586595325028
4	2.7902794674795808	0.9293730100566628
5	1.6379292817907454	0.6819421157537477
6	1.010886644800755	0.5050519714176802
7	0.6910370802158599	0.4027928502774398
8	0.514633814670971	0.3428235651604695
9	0.4015023090660105	0.30089357758184704
>10	2.65879078563802	4.44449285212721
>50	0.4005296593840024	2.35724416346072
>100	0.6790249302033604	14.466639026666423
>500	0.7141893439766777	44.57911211957129
>1k	0.15882926395662716	16.57432977386098
>5k	0.0013945984152289215	0.7299585985752366
>10k+	0.0010846876562891612	4.199136095316228
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	107508	1.3861109639149318	No Hit
CCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCTCTCAGGGAGGCTAA	85134	1.0976408341884678	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	72762	0.9381274505746388	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	20365	0.26256789987840523	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAAC	17137	0.22094898601601914	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC	12150	0.15665111630359063	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	10384	0.13388190878160372	No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA	8793	0.11336899305822819	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03093053728496411	0.0	0.0	0.0	0.0
2	0.060352582338856604	0.0	0.0	0.0	0.0
3	0.0801434846866765	0.0	0.0	0.0	0.0
4	0.09966363201866302	0.0	0.0	0.0	0.0
5	0.12516617589096773	0.0	0.0	0.0	0.0
6	0.1691832056078779	0.0	0.0	0.0	0.0
7	0.20833953813577952	0.0	0.0	0.0	0.0
8	0.26175563483090514	0.0	0.0	0.0	0.0
9	0.3045736066205532	1.2893095992065073E-5	0.0	0.0	0.0
10	0.3482554158416697	1.2893095992065073E-5	0.0	0.0	0.0
11	0.39393565494155625	1.2893095992065073E-5	0.0	0.0	0.0
12	0.44418005002263383	1.2893095992065073E-5	0.0	0.0	0.0
13	0.49784111554160865	1.2893095992065073E-5	0.0	0.0	0.0
14	0.5492458892619722	1.2893095992065073E-5	0.0	0.0	0.0
15	0.605292177539479	1.2893095992065073E-5	0.0	0.0	0.0
16	0.6590434947303982	1.2893095992065073E-5	0.0	0.0	0.0
17	0.7230319301390172	1.2893095992065073E-5	0.0	0.0	0.0
18	0.7807027485115243	1.2893095992065073E-5	0.0	0.0	0.0
19	0.8435436983768495	1.2893095992065073E-5	0.0	0.0	0.0
20	0.9019365301249122	1.2893095992065073E-5	0.0	0.0	0.0
21	0.9604453997369035	1.2893095992065073E-5	0.0	0.0	0.0
22	1.0167624430302438	1.2893095992065073E-5	0.0	0.0	0.0
23	1.0737370342191792	1.2893095992065073E-5	0.0	0.0	0.0
24	1.126585834690654	1.2893095992065073E-5	0.0	0.0	0.0
25	1.1828770917920102	1.2893095992065073E-5	0.0	0.0	0.0
26	1.2429331329230493	1.2893095992065073E-5	0.0	0.0	0.0
27	1.298347659496945	1.2893095992065073E-5	0.0	0.0	0.0
28	1.3543165891984994	1.2893095992065073E-5	0.0	0.0	0.0
29	1.416435525688269	1.2893095992065073E-5	0.0	0.0	0.0
30	1.479444085801491	1.2893095992065073E-5	0.0	0.0	0.0
31	1.5379658485094743	1.2893095992065073E-5	0.0	0.0	0.0
32	1.5979832103525373	1.2893095992065073E-5	0.0	0.0	0.0
33	1.6567112625963936	1.2893095992065073E-5	0.0	0.0	0.0
34	1.7250575644503305	1.2893095992065073E-5	0.0	0.0	0.0
35	1.7877824764517272	1.2893095992065073E-5	0.0	0.0	0.0
36	1.8524284597559415	1.2893095992065073E-5	0.0	0.0	0.0
37	1.9132580866465045	1.2893095992065073E-5	0.0	0.0	0.0
38	1.9711609807468686	1.2893095992065073E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCAT	10210	0.0	70.76041	44
AATCGCA	1150	0.0	67.67997	44
ATACGAC	95	0.0	57.8318	44
GCTCGAA	60	3.1457603E-8	53.414093	44
CGACGAA	115	0.0	51.109486	43
CCTTAGC	12680	0.0	49.355164	43
TAAGCCA	3895	0.0	48.31071	44
ACTTCTA	2325	0.0	47.457314	44
TACGGGG	75	0.0	45.99889	42
ACCTTAG	12525	0.0	44.602005	42
TACGTCC	85	0.0	44.349926	40
ACGATAG	170	0.0	43.090363	44
AGGCTAA	11135	0.0	43.087994	42
GAGGCTA	11155	0.0	42.74655	41
CGCTACT	8745	0.0	42.43575	33
GACGCTA	8685	0.0	42.435318	31
CGACGGG	995	0.0	41.87236	44
CCTATCA	10905	0.0	41.86088	43
GACAACC	14635	0.0	41.793434	38
CCCCTAT	9490	0.0	41.71252	41
>>END_MODULE