FastQCFastQC Report
Tue 24 May 2016
ERR841226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382416
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC11090.2899983264298565No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG10480.27404711099953977No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA10020.2620183255930714No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG10020.2620183255930714No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC9570.2502510355215263No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG7240.18932262248441487No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA6120.1600351449730137No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT6020.15742019162378143No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG5800.1516672942554705No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC5750.15035981758085437No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA5730.1498368269110079No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG5630.14722187356177566No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC5490.1435609388728505No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG5400.14120748085854148No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT5070.13257813480607505No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC4860.12708673277268734No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT4650.12159533073929961No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT4570.11950336805991381No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT4520.1181958913852977No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG4420.11558093803606542No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT4380.11453495669637255No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA4220.11035103133760094No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA4120.10773607798836868No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT4010.10485962930421322No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT3990.10433663863436676No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG3980.10407514329944353No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC3970.10381364796452032No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA3930.10276766662482742No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT3920.10250617128990419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTGG755.456968E-12100.00214444
ACTTCTA1600.099.611544
AATCGCA306.9514656E-493.7520144
CTATCAT1450.090.5191844
ATGTTCT1100.085.229144
CGCTACC801.0404619E-982.03300544
AACGACA453.274181E-1181.8686643
TTCAGGA2100.071.430144
TCGTGCG1450.063.51879543
GTGTTAG1650.058.61052343
TAACCGG250.005667550555.2613543
GACCTTA250.005667550555.2613543
CGTGCGC1401.4260877E-953.57257544
ATCACCC702.9595508E-453.57257544
GTTCAGG3550.051.8885943
TCGCAAA253.6762826E-451.3110542
TGTGTTA2750.051.3110542
AAGGTCT905.456968E-1251.16791543
TACGCTA1100.049.56181342
GACAGTT150.00892171949.2806839
Produced by FastQC (version 0.11.5)