##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841226.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382416
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01682199489561	37.0	35.0	39.0	30.0	39.0
2	36.775731140956445	38.0	35.0	39.0	32.0	39.0
3	37.023252165181376	39.0	36.0	39.0	33.0	39.0
4	37.44870769005481	39.0	37.0	40.0	33.0	41.0
5	38.005946403916155	39.0	37.0	41.0	33.0	41.0
6	38.04257405547885	39.0	37.0	41.0	33.0	41.0
7	37.888804338730594	39.0	37.0	41.0	33.0	41.0
8	37.87712595707293	39.0	37.0	40.0	33.0	41.0
9	37.80597569139366	39.0	36.0	40.0	33.0	41.0
10	37.572781996569184	39.0	36.0	40.0	32.0	41.0
11	37.524891740931345	39.0	36.0	40.0	32.0	41.0
12	37.44747604702732	39.0	36.0	40.0	32.0	41.0
13	37.322761076942385	39.0	36.0	40.0	32.0	41.0
14	37.25088385423204	39.0	36.0	40.0	32.0	41.0
15	37.22028890004602	38.0	36.0	40.0	32.0	41.0
16	37.105570896615205	38.0	35.0	40.0	31.0	41.0
17	37.04210074892264	38.0	35.0	40.0	31.0	41.0
18	36.92363813229572	38.0	35.0	40.0	31.0	41.0
19	36.86496380904565	38.0	35.0	40.0	31.0	41.0
20	36.70979770720891	38.0	35.0	40.0	31.0	41.0
21	36.58883258022677	38.0	35.0	40.0	31.0	41.0
22	36.60108102171457	38.0	35.0	40.0	31.0	41.0
23	36.46431111668968	38.0	35.0	40.0	30.0	41.0
24	36.441751914145854	38.0	35.0	40.0	30.0	41.0
25	36.425019236006634	38.0	35.0	40.0	30.0	41.0
26	36.40377534809831	38.0	35.0	40.0	31.0	41.0
27	36.41073363193894	38.0	35.0	40.0	31.0	41.0
28	36.3110436215561	38.0	34.0	40.0	30.0	41.0
29	36.308883157208015	38.0	34.0	40.0	31.0	41.0
30	36.32916505867883	38.0	34.0	40.0	31.0	41.0
31	36.22271593170923	37.0	34.0	40.0	31.0	41.0
32	36.217311499519205	37.0	34.0	40.0	31.0	41.0
33	36.12542961212163	37.0	34.0	40.0	31.0	41.0
34	36.07383748725599	37.0	34.0	40.0	31.0	41.0
35	35.95075928147888	37.0	34.0	40.0	31.0	41.0
36	35.832148012533885	36.0	34.0	40.0	30.0	41.0
37	35.75850575733357	36.0	34.0	39.0	30.0	41.0
38	35.6825581812314	36.0	34.0	39.0	30.0	41.0
39	35.54021757170663	36.0	34.0	39.0	30.0	41.0
40	35.42101764784799	36.0	34.0	39.0	30.0	41.0
41	35.26101506716222	35.0	33.0	39.0	30.0	40.0
42	35.13957540894527	35.0	33.0	39.0	30.0	40.0
43	35.037827167992106	35.0	33.0	39.0	30.0	40.0
44	34.90840578408766	35.0	33.0	38.0	29.0	40.0
45	34.86036585961383	35.0	33.0	38.0	30.0	40.0
46	34.785108886407144	35.0	33.0	38.0	29.0	40.0
47	34.77311579802124	35.0	33.0	38.0	29.0	40.0
48	34.77961907903919	35.0	33.0	38.0	30.0	40.0
49	34.82208895853423	35.0	33.0	38.0	30.0	40.0
50	35.16983469523107	35.0	33.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	6.0
23	19.0
24	42.0
25	131.0
26	314.0
27	787.0
28	1814.0
29	3880.0
30	7410.0
31	12516.0
32	19517.0
33	27782.0
34	37653.0
35	42630.0
36	51790.0
37	57459.0
38	53962.0
39	44189.0
40	20513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.570363164721144	15.266359148152798	35.16327768712606
2	25.6699510480733	23.85935734906489	30.600184092715786	19.87050751014602
3	29.161175264633275	16.973923685201456	26.13018283753818	27.734718212627087
4	18.60879251914146	18.298397556587588	30.87684615706456	32.21596376720639
5	21.287812225429896	21.959593740847662	28.124607756997616	28.62798627672482
6	28.015302706999705	18.593102799046065	26.267990879042717	27.123603614911513
7	28.91092422911175	18.28846073386051	25.41708505920254	27.383529977825194
8	27.687387557005984	16.575143299443535	26.225890130120078	29.5115790134304
9	28.706434877201794	18.263880172377725	24.393853813647965	28.63583113677252
10	27.26271913309067	19.25050207104305	27.599002133801932	25.88777666206435
11	30.135768377892134	20.283931634659638	24.06855361700347	25.511746370444747
12	29.813344629931805	17.474425756244507	24.500805405631564	28.211424208192128
13	27.24179950629681	18.107767457428558	24.15353960085352	30.496893435421114
14	26.36448265762939	22.06393037948203	21.80060457721434	29.770982385674237
15	25.90242040082005	23.15854985147065	21.915924019915483	29.02310572779382
16	26.047550311702437	21.98626626500983	23.03590853939166	28.93027488389607
17	29.937816409355257	21.391364378059496	19.043136270448933	29.627682942136314
18	25.859012175222794	20.807706790510856	21.962208694196896	31.371072340069457
19	27.650255219446883	20.664668842307854	24.470994937450318	27.21408100079495
20	29.488305928622232	18.32742353876407	24.401175683025812	27.783094849587886
21	28.556859545625706	25.00627588803816	20.473254257144053	25.963610309192088
22	31.114283921174845	20.30458976611857	22.83586460817539	25.745261704531192
23	28.399700849336845	22.88711769382034	23.62793397765784	25.085247479184968
24	31.52143215765031	22.579076189280784	20.665453328312623	25.234038324756288
25	29.303511133086722	22.32725272807558	21.41265002102079	26.956586117816904
26	30.088776749836466	24.350194240858663	22.029449851148758	23.531579158156113
27	30.9832964118817	22.41347713834495	21.292358607010225	25.310867842763123
28	29.817914391164013	22.317403679845288	23.24322115842946	24.62146077056124
29	30.374666582251304	21.72025665940878	23.5293146114737	24.37576214686622
30	28.616445620396412	22.81491805508897	23.166963276796942	25.401673047717676
31	27.193518544475904	22.3158868548681	25.787614588881723	24.70298001177427
32	27.394083375756544	21.218960348435996	25.46722099666271	25.919735279144746
33	26.271708971328746	20.108987314310507	27.12636590963587	26.492937804724875
34	27.066833322773327	19.810668678827955	28.70872237358232	24.4137756248164
35	28.185074678899614	20.224748479839167	24.856263817993245	26.733913023267974
36	27.228051027473622	20.795925313053782	23.93555845039843	28.04046520907416
37	24.801527395435574	23.22943492289021	26.789509516620786	25.179528165053426
38	28.99588816772451	21.718901280166385	23.450293446169663	25.834917105939446
39	27.20068802148558	21.761395355854972	25.519863602758118	25.51805301990132
40	27.074262437037312	21.201505006524947	24.231946629590333	27.492285926847416
41	25.809725028144904	22.23617044245331	23.892787193683205	28.061317335718584
42	25.287776299599642	23.241332358984927	26.20528771384137	25.265603627574063
43	25.34527808883912	23.39191831001955	23.93791004738677	27.324893553754563
44	23.333579200887687	24.427155323086	25.354931194499407	26.8843342815269
45	25.866710942256166	21.279304091910912	26.437735824486353	26.416249141346565
46	24.777972771750694	21.776949053392894	24.791932567538822	28.653145607317594
47	25.002314963209894	22.287753488471484	26.091772264603858	26.61815928371477
48	25.060166563040596	22.121838876270477	24.54076734044758	28.27722722024134
49	26.403688524590162	21.692381870781098	23.67827868852459	28.225650916104144
50	23.256541488869146	24.729393215121444	24.688098306908763	27.325966989100646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	841.0
1	804.0
2	509.0
3	246.5
4	234.0
5	190.0
6	129.0
7	105.5
8	113.0
9	133.0
10	157.0
11	169.0
12	160.5
13	165.0
14	202.0
15	236.0
16	256.5
17	267.0
18	270.5
19	289.5
20	342.0
21	437.5
22	525.0
23	625.5
24	755.0
25	916.5
26	1087.5
27	1245.0
28	1432.0
29	1712.5
30	2141.0
31	3004.5
32	4011.5
33	4349.5
34	4650.0
35	5593.5
36	6471.5
37	7129.0
38	7707.5
39	8482.5
40	9731.0
41	12296.0
42	15889.5
43	18124.0
44	18684.5
45	19400.0
46	21144.5
47	22871.0
48	26193.0
49	30282.5
50	33574.5
51	36444.5
52	38004.5
53	37851.0
54	35920.0
55	33538.5
56	31540.0
57	30152.0
58	28610.0
59	26046.0
60	24184.5
61	23854.5
62	23449.0
63	22441.5
64	20698.5
65	18821.0
66	17382.5
67	16203.0
68	14486.0
69	12539.5
70	11117.0
71	9964.5
72	8895.5
73	7883.0
74	6783.0
75	5659.5
76	4711.0
77	3662.0
78	2918.5
79	2481.5
80	1963.5
81	1425.5
82	1024.5
83	754.0
84	568.0
85	428.5
86	302.5
87	214.5
88	156.5
89	119.5
90	87.5
91	57.0
92	36.5
93	29.5
94	25.5
95	19.0
96	15.0
97	14.0
98	7.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	4219.0
25	3662.0
26	3477.0
27	3923.0
28	3852.0
29	3102.0
30	3471.0
31	3130.0
32	3264.0
33	3094.0
34	3015.0
35	3371.0
36	3006.0
37	3186.0
38	3259.0
39	3413.0
40	3757.0
41	4001.0
42	4092.0
43	4301.0
44	4654.0
45	6303.0
46	20082.0
47	44355.0
48	70507.0
49	86007.0
50	79913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.294791914787055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.01629816349179	17.288701742805213
2	12.702272713164003	7.442208719540343
3	7.1807010924200565	6.31071432914189
4	4.803493236859359	5.628693393515275
5	3.3159368585937266	4.856984013753794
6	2.4704459910147003	4.342272074609733
7	1.7776865674486517	3.645387065818422
8	1.4998640495889228	3.515056418654188
9	1.1439638473414273	3.01609645793157
>10	5.674423625081262	27.448870414159014
>50	0.25693790145272855	5.193590227075024
>100	0.1443573368586001	8.400918774639816
>500	0.009986985568833972	1.8038064689928208
>1k	0.0036316311159396257	1.1066998993628911
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	1109	0.2899983264298565	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	1048	0.27404711099953977	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	1002	0.2620183255930714	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	1002	0.2620183255930714	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	957	0.2502510355215263	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	724	0.18932262248441487	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	612	0.1600351449730137	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	602	0.15742019162378143	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	580	0.1516672942554705	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	575	0.15035981758085437	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	573	0.1498368269110079	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	563	0.14722187356177566	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	549	0.1435609388728505	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	540	0.14120748085854148	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	507	0.13257813480607505	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	486	0.12708673277268734	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	465	0.12159533073929961	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	457	0.11950336805991381	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	452	0.1181958913852977	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	442	0.11558093803606542	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	438	0.11453495669637255	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	422	0.11035103133760094	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	412	0.10773607798836868	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	401	0.10485962930421322	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	399	0.10433663863436676	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	398	0.10407514329944353	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	397	0.10381364796452032	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	393	0.10276766662482742	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGT	392	0.10250617128990419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.20605832391950127	0.0	0.0	0.0	0.0
2	0.6124220743901929	2.61495334923225E-4	0.0	0.0	0.0
3	0.7988682481904523	2.61495334923225E-4	0.0	0.0	0.0
4	0.9429521777331492	2.61495334923225E-4	0.0	0.0	0.0
5	1.1890192878959038	2.61495334923225E-4	0.0	0.0	0.0
6	1.3474854608593783	2.61495334923225E-4	0.0	0.0	0.0
7	1.4724802309526799	2.61495334923225E-4	0.0	0.0	0.0
8	1.5721099535584284	2.61495334923225E-4	0.0	0.0	0.0
9	1.6584034140830928	2.61495334923225E-4	0.0	0.0	0.0
10	1.7240387431488222	2.61495334923225E-4	0.0	0.0	0.0
11	1.8116396803481025	2.61495334923225E-4	0.0	0.0	0.0
12	1.8788439814233715	2.61495334923225E-4	0.0	0.0	0.0
13	1.9206832350110874	2.61495334923225E-4	0.0	0.0	0.0
14	1.967752395297268	2.61495334923225E-4	0.0	0.0	0.0
15	2.0454165097694657	2.61495334923225E-4	0.0	0.0	0.0
16	2.084117819338103	2.61495334923225E-4	0.0	0.0	0.0
17	2.1264800635956655	2.61495334923225E-4	0.0	0.0	0.0
18	2.1821785699343126	2.61495334923225E-4	0.0	0.0	0.0
19	2.3939897912221246	2.61495334923225E-4	0.0	0.0	0.0
20	2.427722689427221	2.61495334923225E-4	0.0	0.0	0.0
21	2.539904188109284	2.61495334923225E-4	0.0	0.0	0.0
22	2.5574243755491404	2.61495334923225E-4	0.0	0.0	0.0
23	2.580697460357307	2.61495334923225E-4	0.0	0.0	0.0
24	2.6018785824860884	2.61495334923225E-4	0.0	0.0	0.0
25	2.632473536672106	2.61495334923225E-4	0.0	0.0	0.0
26	2.8479456926488433	2.61495334923225E-4	0.0	0.0	0.0
27	2.867296347433162	2.61495334923225E-4	0.0	0.0	0.0
28	2.8876929835571734	2.61495334923225E-4	0.0	0.0	0.0
29	2.9073051336764153	2.61495334923225E-4	0.0	0.0	0.0
30	2.9237793397765786	2.61495334923225E-4	0.0	0.0	0.0
31	2.938684573867202	2.61495334923225E-4	0.0	0.0	0.0
32	2.953589807957826	2.61495334923225E-4	0.0	0.0	0.0
33	2.9671875653738335	2.61495334923225E-4	0.0	0.0	0.0
34	2.9792163507803022	2.61495334923225E-4	0.0	0.0	0.0
35	2.9925526128613864	2.61495334923225E-4	0.0	0.0	0.0
36	3.0043199029329317	2.61495334923225E-4	0.0	0.0	0.0
37	3.0158256976695537	2.61495334923225E-4	0.0	0.0	0.0
38	3.0252395297267896	2.61495334923225E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATTGG	75	5.456968E-12	100.002144	44
ACTTCTA	160	0.0	99.6115	44
AATCGCA	30	6.9514656E-4	93.75201	44
CTATCAT	145	0.0	90.51918	44
ATGTTCT	110	0.0	85.2291	44
CGCTACC	80	1.0404619E-9	82.033005	44
AACGACA	45	3.274181E-11	81.86866	43
TTCAGGA	210	0.0	71.4301	44
TCGTGCG	145	0.0	63.518795	43
GTGTTAG	165	0.0	58.610523	43
TAACCGG	25	0.0056675505	55.26135	43
GACCTTA	25	0.0056675505	55.26135	43
CGTGCGC	140	1.4260877E-9	53.572575	44
ATCACCC	70	2.9595508E-4	53.572575	44
GTTCAGG	355	0.0	51.88859	43
TCGCAAA	25	3.6762826E-4	51.31105	42
TGTGTTA	275	0.0	51.31105	42
AAGGTCT	90	5.456968E-12	51.167915	43
TACGCTA	110	0.0	49.561813	42
GACAGTT	15	0.008921719	49.28068	39
>>END_MODULE