FastQCFastQC Report
Tue 24 May 2016
ERR841225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241614
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG12530.5185957767347918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA11930.49376277864693274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA10490.43416358323607074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA10420.4312664001258205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9400.38905030337645996No Hit
AAAAAAAAAAAAAAAAAAAAAAAA8320.3443509068183135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA8110.3356593574875628No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG7030.29095996092941634No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA6950.28764889451770176No Hit
AAGCAGTGGTATCAACGCAGAGTG5560.2301191156141614No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT5180.2143915501585173Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT5030.20818330063655252No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG4910.2032167010189807Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.20238893441605205No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG4510.18666136896040791Illumina Single End Adapter 2 (100% over 34bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC4340.1796253528355145No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG4340.1796253528355145No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA4340.1796253528355145No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC4210.17424486991647836No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC4100.16969215360037082No Hit
AAGCAGTGGTATCAACGCAGAGCG3990.16513943728426334No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG3770.1560340046520483No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA3740.15479235474765535No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGAAGCAGTGGT3520.14568692211544032No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG3500.1448591555125117No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG3450.1427897390051901Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCA3170.13120100656418915No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG3120.12913159005686758Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA2960.12250945723343847No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC2920.12085392402758119Illumina Single End Adapter 2 (100% over 25bp)
CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAACACA2870.1187845075202596No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGG2800.11588732441000936No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAC2720.1125762579982948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.11133460809390183No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA2640.10926519158658025No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2640.10926519158658025Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG2580.10678189177779433No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA2570.10636800847633002No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG2530.10471247527047273Illumina Single End Adapter 2 (100% over 23bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2530.10471247527047273Illumina Single End Adapter 2 (100% over 34bp)
AGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAG2490.10305694206461546No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAACC159.288706E-5155.403944
TTAATAG100.0057502813155.4038844
GGCATAA202.9206788E-4116.5529244
ACCGGGA257.094108E-493.2423444
AACGACA550.085.1696143
AATCCCG401.0495569E-981.9757543
GTAACAG301.4751913E-678.0721443
CTATCAT501.5099849E-677.7019544
CGTGTGC300.001463514877.7019544
CTTAGCC604.8879883E-877.7019544
TTAAGCC255.6263874E-574.9492643
GTAGGCT200.002185823470.2649343
TAAGCCA458.40626E-569.068444
AATCCTG551.6916601E-1068.1356943
TGAACAC452.2332279E-762.45771443
ATGTTCT501.4160368E-462.16155644
ACGTAGG150.004375002358.99453741
TGTGTTA1150.058.4375842
GATTTTC400.004578281758.2764644
AAAACGC400.004578281758.2764644
Produced by FastQC (version 0.11.5)