##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841225.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 241614 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.647963280273494 37.0 35.0 39.0 30.0 39.0 2 36.48118072628242 38.0 35.0 39.0 32.0 39.0 3 36.600606752919944 38.0 35.0 39.0 32.0 39.0 4 36.882419892887 38.0 35.0 39.0 33.0 40.0 5 37.68832931866531 39.0 36.0 40.0 33.0 41.0 6 37.688842533959125 39.0 36.0 40.0 33.0 41.0 7 37.37280538379399 39.0 35.0 40.0 32.0 41.0 8 37.485278170966915 39.0 36.0 40.0 33.0 41.0 9 37.509370317945155 39.0 36.0 40.0 33.0 41.0 10 37.03184004238165 38.0 35.0 40.0 32.0 41.0 11 37.16855811335436 38.0 35.0 40.0 32.0 41.0 12 37.147441787313646 38.0 35.0 40.0 32.0 41.0 13 37.097742680473814 38.0 35.0 40.0 32.0 41.0 14 36.989681889294495 38.0 35.0 40.0 32.0 41.0 15 36.862106500451134 38.0 35.0 40.0 31.0 41.0 16 36.70295595453906 38.0 35.0 40.0 31.0 41.0 17 36.55630054549819 38.0 35.0 40.0 31.0 41.0 18 36.540411565554976 38.0 35.0 40.0 31.0 41.0 19 36.451861233206685 38.0 35.0 40.0 31.0 41.0 20 36.279255341164 38.0 34.0 40.0 30.0 41.0 21 36.097568849487196 38.0 34.0 40.0 30.0 41.0 22 36.01171289743144 38.0 34.0 40.0 30.0 41.0 23 35.75701739137633 37.0 34.0 40.0 30.0 41.0 24 35.89304427723559 37.0 34.0 40.0 30.0 41.0 25 35.91956280275867 38.0 34.0 40.0 30.0 41.0 26 35.99379258202813 38.0 34.0 40.0 30.0 41.0 27 36.043239725159765 38.0 34.0 40.0 30.0 41.0 28 35.84964178105122 37.0 34.0 40.0 30.0 41.0 29 35.93818435652237 37.0 34.0 40.0 30.0 41.0 30 36.09603681851679 37.0 34.0 40.0 30.0 41.0 31 36.04705521617095 37.0 34.0 40.0 30.0 41.0 32 36.02831682315857 37.0 34.0 40.0 30.0 41.0 33 35.96379523774849 37.0 34.0 40.0 30.0 41.0 34 35.91493945325949 37.0 34.0 40.0 30.0 41.0 35 35.822633432782204 37.0 34.0 40.0 30.0 41.0 36 35.72903522840534 36.0 34.0 40.0 30.0 41.0 37 35.652734884007266 36.0 34.0 39.0 30.0 41.0 38 35.55978436208852 36.0 34.0 39.0 30.0 41.0 39 35.43024749100249 36.0 34.0 39.0 30.0 41.0 40 35.31771185985276 35.0 33.0 39.0 29.0 41.0 41 35.16607742957843 35.0 33.0 39.0 29.0 41.0 42 35.027709729862146 35.0 33.0 39.0 29.0 40.0 43 34.91808945396753 35.0 33.0 39.0 29.0 40.0 44 34.777991428240476 35.0 33.0 38.0 29.0 40.0 45 34.736060029092116 35.0 33.0 38.0 29.0 40.0 46 34.66040771883129 35.0 33.0 38.0 29.0 40.0 47 34.60690867774103 35.0 33.0 38.0 29.0 40.0 48 34.66186113161666 35.0 33.0 38.0 29.0 40.0 49 34.61470207715738 35.0 33.0 38.0 29.0 40.0 50 34.84050912396909 35.0 33.0 38.0 29.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 9.0 22 10.0 23 29.0 24 84.0 25 176.0 26 371.0 27 834.0 28 1655.0 29 3293.0 30 5868.0 31 9338.0 32 14016.0 33 20276.0 34 27515.0 35 26158.0 36 31277.0 37 33838.0 38 30868.0 39 25142.0 40 10857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 56.37131954274173 15.331065252841308 28.297615204416964 2 25.46499788919516 34.35810838775899 24.334682592896108 15.842211130149744 3 32.64380375309378 20.097759235805874 23.059922024386005 24.198514986714343 4 18.386351784250913 22.922098884998384 26.77618018823413 31.915369142516575 5 20.707409338862814 31.788720852268494 23.064888624003576 24.438981184865117 6 30.579353845389758 23.393511965366244 23.66874436084002 22.358389828403983 7 30.309088049533557 21.331131474169542 26.164460668669864 22.195319807627044 8 32.29117518024618 20.632082577996307 23.251549992963984 23.82519224879353 9 32.234059284644104 22.820697476139628 22.78344797900784 22.161795260208432 10 25.210873542096067 22.458963470659814 29.197811385101858 23.132351602142258 11 27.050584817104966 29.712268328822006 21.290570910626037 21.946575943446987 12 27.789366510218777 24.125671525656625 25.846184409843804 22.238777554280794 13 24.465469716158832 21.918431878947413 20.803430264802536 32.81266814009122 14 26.208746181926546 30.091385432963317 20.02864072446133 23.671227660648803 15 23.535060054467042 30.114562897845325 22.266507735478903 24.08386931220873 16 25.638828875810177 24.36655160710886 20.875445959257327 29.119173557823636 17 34.35603897125167 22.633622223877754 17.632256408982926 25.37808239588766 18 26.35484698734345 21.846002301191156 21.342306323309078 30.456844388156316 19 25.088777968164095 28.93333995546616 22.11750974695175 23.860372329418 20 33.47488142243413 21.662651998642463 21.56166447308517 23.30080210583824 21 27.17723310735305 31.116160487388978 19.377188407956492 22.329417997301483 22 31.43195344640625 24.474989032092513 20.85185461107386 23.241202910427376 23 26.949183408246213 25.720777769500113 24.006059251533436 23.32397957072024 24 32.89089208406797 24.58218480717177 19.072984181380217 23.453938927380037 25 26.78762231766907 25.505424844054208 20.588645203795913 27.11830763448081 26 26.41567791877518 29.500915003479587 20.321239249783062 23.762167827962163 27 30.69107555485666 24.158159810949055 21.226297650404266 23.924466983790015 28 27.79734404642339 24.149983260796787 24.664211583528626 23.3884611092512 29 28.889882473173223 23.935141251186216 23.39951821300825 23.77545806263231 30 28.058824347310217 23.522323311441827 23.109829858037365 25.30902248321059 31 26.56933273580678 24.07953392928745 26.056088874212797 23.295044460692974 32 26.26194018071108 22.613757575467684 25.725259849901704 25.399042393919537 33 24.697201388383213 22.28112332823612 27.217165465181193 25.804509818199474 34 27.104380416498618 22.732817218686368 27.568195008705747 22.594607356109268 35 27.84052924096229 22.17493688821481 25.02818826769375 24.956345603129147 36 26.401319484146455 24.683156845582914 23.48357980905931 25.431943861211316 37 25.397330268922563 25.672819428911488 26.300750028215838 22.629100273950115 38 29.05075608563073 24.336798942624913 22.3953292328827 24.217115738861654 39 25.895391054809508 25.766439945248624 23.873923865996545 24.464245133945322 40 27.040679241227362 26.063195228115426 21.885646730076843 25.010478800580366 41 24.81844758339936 26.009125433731317 22.603753995792463 26.56867298707686 42 23.937710175266684 27.782108099681537 23.606440549629202 24.673741175422577 43 25.221364513565668 27.075718015665796 22.828357361789077 24.874560108979452 44 23.527742383875825 25.66025715278582 25.374145719911965 25.437854743426385 45 25.331425395286132 24.641965964592767 25.482207689305692 24.544400950815408 46 24.063879983062126 25.425564091706494 23.302885487871272 27.20767043736011 47 24.53075707276201 25.1921721964441 24.824648821576606 25.452421909217282 48 25.64809587910895 24.985887359416388 23.098412147003053 26.267604614471608 49 27.083524342165582 23.80835956521296 22.81002006866131 26.29809602396015 50 23.590817585557502 26.73225534125593 24.17851808558997 25.498408987596598 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8136.0 1 8042.0 2 4650.0 3 1349.5 4 1322.5 5 1006.0 6 464.5 7 218.0 8 202.5 9 169.5 10 149.5 11 146.0 12 143.5 13 160.0 14 202.0 15 228.5 16 244.0 17 281.5 18 340.0 19 401.0 20 457.0 21 564.5 22 760.0 23 1027.5 24 1269.5 25 1453.5 26 1626.5 27 1776.0 28 1891.0 29 1995.0 30 2123.0 31 2429.0 32 2885.0 33 3484.5 34 4038.0 35 4129.5 36 4225.0 37 5128.0 38 6245.5 39 6673.0 40 7065.0 41 8806.0 42 11207.0 43 12659.0 44 12887.0 45 12673.0 46 13230.0 47 14651.0 48 17479.5 49 20281.0 50 23443.0 51 27364.5 52 28865.0 53 27651.0 54 25013.0 55 20897.0 56 17083.0 57 15378.0 58 14334.0 59 13194.0 60 12706.5 61 12468.5 62 11822.5 63 11378.5 64 10777.0 65 9655.5 66 8738.0 67 8248.0 68 7431.5 69 6442.5 70 5711.0 71 5054.5 72 4550.5 73 4186.0 74 3561.0 75 2824.0 76 2372.0 77 1934.5 78 1517.0 79 1292.5 80 1027.5 81 687.0 82 453.0 83 349.5 84 289.5 85 238.0 86 186.0 87 130.0 88 91.5 89 80.5 90 73.0 91 54.0 92 35.5 93 30.0 94 18.5 95 8.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 4833.0 25 3995.0 26 3853.0 27 4908.0 28 4841.0 29 3423.0 30 3776.0 31 2922.0 32 3068.0 33 2681.0 34 2876.0 35 2785.0 36 2727.0 37 2752.0 38 2955.0 39 3129.0 40 3085.0 41 3393.0 42 3432.0 43 3520.0 44 3542.0 45 3808.0 46 7769.0 47 19367.0 48 40011.0 49 36567.0 50 61596.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.40995968776644 #Duplication Level Percentage of deduplicated Percentage of total 1 63.16165860012515 20.470668090425225 2 12.941371780300612 8.388586754078819 3 6.533260117230899 6.352280910874369 4 4.189919164314812 5.431804448417724 5 2.875860395622358 4.660325974488233 6 1.997267166409133 3.883880900941171 7 1.560524601887443 3.540357760725786 8 1.159538738554663 3.006448301836814 9 0.910518855274752 2.655889145496536 >10 4.297189267881543 23.195261863964838 >50 0.21709425721838405 5.033234829107585 >100 0.1404727546707191 9.2031090913606 >500 0.01021620033968866 2.3003633895386857 >1k 0.00510810016984433 1.8777885387436157 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTGCAG 1253 0.5185957767347918 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 1193 0.49376277864693274 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 1049 0.43416358323607074 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 1042 0.4312664001258205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 940 0.38905030337645996 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 832 0.3443509068183135 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 811 0.3356593574875628 No Hit ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG 703 0.29095996092941634 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGA 695 0.28764889451770176 No Hit AAGCAGTGGTATCAACGCAGAGTG 556 0.2301191156141614 No Hit AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT 518 0.2143915501585173 Illumina Single End Adapter 2 (100% over 32bp) AAGCAGTGGTATCAACGCAGAGTGCAGT 503 0.20818330063655252 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 491 0.2032167010189807 Illumina Single End Adapter 1 (100% over 32bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 489 0.20238893441605205 No Hit AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 451 0.18666136896040791 Illumina Single End Adapter 2 (100% over 34bp) CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC 434 0.1796253528355145 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG 434 0.1796253528355145 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 434 0.1796253528355145 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGC 421 0.17424486991647836 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC 410 0.16969215360037082 No Hit AAGCAGTGGTATCAACGCAGAGCG 399 0.16513943728426334 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 377 0.1560340046520483 No Hit ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA 374 0.15479235474765535 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTTGGGGAAGCAGTGGT 352 0.14568692211544032 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTG 350 0.1448591555125117 No Hit AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG 345 0.1427897390051901 Illumina Single End Adapter 2 (100% over 30bp) AAGCAGTGGTATCAACGCAGAGTGCA 317 0.13120100656418915 No Hit AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 312 0.12913159005686758 Illumina Single End Adapter 2 (100% over 34bp) AAGCAGTGGTATCAACGCAGAGTGCAGTGCTA 296 0.12250945723343847 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC 292 0.12085392402758119 Illumina Single End Adapter 2 (100% over 25bp) CAGTTGACACAAAATAAACTACGAAAGTGGCTTTAACATATCTGAACACA 287 0.1187845075202596 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGG 280 0.11588732441000936 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAC 272 0.1125762579982948 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 269 0.11133460809390183 No Hit CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA 264 0.10926519158658025 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 264 0.10926519158658025 Illumina Single End Adapter 1 (100% over 32bp) ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG 258 0.10678189177779433 No Hit ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA 257 0.10636800847633002 No Hit AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG 253 0.10471247527047273 Illumina Single End Adapter 2 (100% over 23bp) AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 253 0.10471247527047273 Illumina Single End Adapter 2 (100% over 34bp) AGCACATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAG 249 0.10305694206461546 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5856448715720115 0.0 0.0 0.0 0.0 2 1.778042663090715 0.0 0.0 0.0 0.0 3 2.3620320014568694 0.0 0.0 0.0 0.0 4 2.7345269727747565 0.0 0.0 0.0 0.0 5 3.628101020636221 0.0 0.0 0.0 0.0 6 3.9302358307051746 0.0 0.0 0.0 0.0 7 4.119794382775832 0.0 0.0 0.0 0.0 8 4.287831003170346 0.0 0.0 0.0 0.0 9 4.419859776337464 0.0 0.0 0.0 0.0 10 4.553544082710439 0.0 0.0 0.0 0.0 11 4.716200220185916 0.0 0.0 0.0 0.0 12 4.8138766793314955 0.0 0.0 0.0 0.0 13 4.894170039815574 0.0 0.0 0.0 0.0 14 5.013368430637297 0.0 0.0 0.0 0.0 15 5.209962998832849 0.0 0.0 0.0 0.0 16 5.278667626875926 0.0 0.0 0.0 0.0 17 5.388346701763971 0.0 0.0 0.0 0.0 18 5.492231410431515 0.0 0.0 0.0 0.0 19 6.079531815209384 0.0 0.0 0.0 0.0 20 6.153203042870032 0.0 0.0 0.0 0.0 21 6.43174650475552 0.0 0.0 0.0 0.0 22 6.483895800740023 0.0 0.0 0.0 0.0 23 6.53728674662892 0.0 0.0 0.0 0.0 24 6.585711092900246 0.0 0.0 0.0 0.0 25 6.656899020752109 0.0 0.0 0.0 0.0 26 7.0906487206867155 0.0 0.0 0.0 0.0 27 7.132037050833147 0.0 0.0 0.0 0.0 28 7.176322564089829 0.0 0.0 0.0 0.0 29 7.219780310743583 0.0 0.0 0.0 0.0 30 7.253304858162193 0.0 0.0 0.0 0.0 31 7.285173872374945 0.0 0.0 0.0 0.0 32 7.318698419793555 0.0 0.0 0.0 0.0 33 7.34891190080045 0.0 0.0 0.0 0.0 34 7.370847715778059 0.0 0.0 0.0 0.0 35 7.391127997549811 0.0 0.0 0.0 0.0 36 7.410994396020098 0.0 0.0 0.0 0.0 37 7.4304469111889215 0.0 0.0 0.0 0.0 38 7.438724577218207 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAAACC 15 9.288706E-5 155.4039 44 TTAATAG 10 0.0057502813 155.40388 44 GGCATAA 20 2.9206788E-4 116.55292 44 ACCGGGA 25 7.094108E-4 93.24234 44 AACGACA 55 0.0 85.16961 43 AATCCCG 40 1.0495569E-9 81.97575 43 GTAACAG 30 1.4751913E-6 78.07214 43 CTATCAT 50 1.5099849E-6 77.70195 44 CGTGTGC 30 0.0014635148 77.70195 44 CTTAGCC 60 4.8879883E-8 77.70195 44 TTAAGCC 25 5.6263874E-5 74.94926 43 GTAGGCT 20 0.0021858234 70.26493 43 TAAGCCA 45 8.40626E-5 69.0684 44 AATCCTG 55 1.6916601E-10 68.13569 43 TGAACAC 45 2.2332279E-7 62.457714 43 ATGTTCT 50 1.4160368E-4 62.161556 44 ACGTAGG 15 0.0043750023 58.994537 41 TGTGTTA 115 0.0 58.43758 42 GATTTTC 40 0.0045782817 58.27646 44 AAAACGC 40 0.0045782817 58.27646 44 >>END_MODULE