##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841224.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5596479
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.18068557033806	38.0	35.0	39.0	31.0	39.0
2	36.8742907817576	38.0	37.0	39.0	32.0	39.0
3	37.11154709952454	39.0	37.0	39.0	33.0	39.0
4	37.53536929201378	39.0	37.0	40.0	33.0	41.0
5	38.080913016916526	39.0	37.0	41.0	33.0	41.0
6	38.112620989018275	40.0	37.0	41.0	33.0	41.0
7	38.059275126378566	40.0	37.0	41.0	33.0	41.0
8	37.95622122409465	39.0	37.0	41.0	33.0	41.0
9	37.89087674589684	39.0	37.0	41.0	33.0	41.0
10	37.73267406167342	39.0	36.0	40.0	33.0	41.0
11	37.61551647026639	39.0	36.0	40.0	33.0	41.0
12	37.54866050600744	39.0	36.0	40.0	32.0	41.0
13	37.39166572410975	39.0	36.0	40.0	32.0	41.0
14	37.32090140961844	39.0	36.0	40.0	32.0	41.0
15	37.24895510194892	38.0	35.0	40.0	32.0	41.0
16	37.16738971056623	38.0	35.0	40.0	31.0	41.0
17	37.132063392000575	38.0	35.0	40.0	31.0	41.0
18	36.97704842634092	38.0	35.0	40.0	31.0	41.0
19	36.853198412787755	38.0	35.0	40.0	31.0	41.0
20	36.73017248880948	38.0	35.0	40.0	31.0	41.0
21	36.54492601508913	38.0	35.0	40.0	31.0	41.0
22	36.51120284736171	38.0	35.0	40.0	31.0	41.0
23	36.46278347510997	38.0	35.0	40.0	31.0	41.0
24	36.373262545968636	38.0	35.0	40.0	30.0	41.0
25	36.343773824799605	38.0	34.0	40.0	30.0	41.0
26	36.25524495717661	38.0	34.0	40.0	30.0	41.0
27	36.22749220663948	37.0	34.0	40.0	31.0	41.0
28	36.216995476651384	37.0	34.0	40.0	31.0	41.0
29	36.17644300340147	37.0	34.0	40.0	31.0	41.0
30	36.16262649551299	37.0	34.0	40.0	31.0	41.0
31	36.08763814272193	37.0	34.0	40.0	31.0	41.0
32	36.04144150230316	37.0	34.0	40.0	31.0	41.0
33	35.94355279721451	37.0	34.0	40.0	31.0	41.0
34	35.8561362396948	36.0	34.0	40.0	31.0	41.0
35	35.765610947175844	36.0	34.0	39.0	31.0	41.0
36	35.62452316487625	36.0	34.0	39.0	30.0	41.0
37	35.528073988437455	36.0	34.0	39.0	30.0	41.0
38	35.46969755102272	35.0	34.0	39.0	30.0	41.0
39	35.32629453220957	35.0	34.0	39.0	30.0	41.0
40	35.1584228365105	35.0	33.0	39.0	30.0	40.0
41	35.00296426059784	35.0	33.0	38.0	30.0	40.0
42	34.871473505154704	35.0	33.0	38.0	30.0	40.0
43	34.755835988860404	35.0	33.0	38.0	29.0	40.0
44	34.62611048226289	35.0	33.0	38.0	29.0	40.0
45	34.563963201934975	35.0	33.0	37.0	29.0	40.0
46	34.47116230596579	35.0	33.0	37.0	29.0	40.0
47	34.45592119206474	35.0	33.0	37.0	29.0	40.0
48	34.45445865735739	35.0	33.0	37.0	29.0	40.0
49	34.48229824372628	35.0	33.0	37.0	29.0	39.0
50	34.92081922985303	35.0	33.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	19.0
22	107.0
23	268.0
24	746.0
25	1890.0
26	5088.0
27	12635.0
28	28734.0
29	59619.0
30	112202.0
31	187917.0
32	288130.0
33	410438.0
34	554462.0
35	649402.0
36	784355.0
37	852334.0
38	767567.0
39	610918.0
40	269642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.31044662188494	18.402213248722994	33.28734012939207
2	28.03309009110907	21.82931446718553	28.031857173054703	22.105738268650697
3	29.504140013747932	17.350855779142567	25.378331626009853	27.766672581099648
4	20.240333252389583	19.163406134464186	28.894738995714984	31.701521617431244
5	23.808183681203843	19.18350805926369	28.002946138098615	29.005362121433855
6	28.216562592301337	18.899561670829105	25.70878940133609	27.175086335533464
7	30.907665337438058	19.196212475736978	22.840539560677346	27.05558262614762
8	27.756058764805513	16.38115679519212	27.807930664977032	28.054853775025336
9	27.324984155216164	18.844902303752058	25.329729638939053	28.500383902092725
10	28.9345676093844	18.89261087194288	26.053881377916365	26.118940140756358
11	31.281346718177627	18.887768541613397	24.202199275651708	25.62868546455727
12	29.70846491159888	17.180355720087576	23.732904206376904	29.37827516193664
13	26.61255407194416	18.20798398421579	26.22141528628983	28.95804665755022
14	26.845057401269617	19.706765628889165	21.232760812646667	32.21541615719455
15	25.7934497744028	20.116666211023038	24.06052090966481	30.029363104909358
16	27.39386675086246	20.961072131245377	24.89726129589694	26.747799821995223
17	28.411774617576512	20.7172938556546	18.435734325099766	32.43519720166912
18	26.290905406774513	21.144526764060046	21.504538835935954	31.060028993229494
19	27.670915945543616	19.52481193979286	26.049128389474884	26.755143725188642
20	28.769713957650872	18.245078736112475	24.60845470875527	28.37675259748138
21	29.73001417498395	21.264816682060275	22.545639856774233	26.459529286181542
22	29.51546856514605	21.065530666692396	24.256554880309565	25.162445887851987
23	29.75749573973207	21.611731233155705	22.22561721396614	26.405155813146088
24	31.686690864023614	21.75523217365776	19.823053030307094	26.735023932011536
25	28.953615252346864	22.78461505980865	22.844144916494898	25.41762477134959
26	30.329704884678076	22.01950140090826	23.24128579311958	24.40950792129408
27	28.817312375983377	23.09096455627631	21.98396074643412	26.107762321306193
28	28.979993794684212	20.705602301608035	24.6436025866369	25.67080131707085
29	33.35848710455819	19.903946415166395	23.768628985306478	22.968937494968934
30	27.70701718505079	22.59633054525613	22.279454992917234	27.417197276775845
31	26.624185141242187	21.01681422839089	28.3524189140549	24.00658171631203
32	27.352458605806447	20.085643225262757	25.795031641202794	26.766866527728013
33	26.76501919217671	19.522980185280144	26.522951342329627	27.189049280213524
34	24.83457216346546	19.592136600394035	30.598108827333192	24.975182408807306
35	28.654093441920274	19.362211130515693	24.46717102836808	27.51652439919595
36	26.893649951524452	19.270048229247134	25.007642272902338	28.82865954632608
37	25.040086816218643	20.38539015531476	26.61784622803436	27.95667680043224
38	29.276481853788326	22.07825435148022	22.241417190416687	26.40384660431477
39	27.388913475621234	21.750089042005822	25.107563558061504	25.75343392431144
40	27.19594222529172	21.203107968879138	22.856828196099702	28.74412160972944
41	27.113031493630825	22.065738734468106	22.66081649352786	28.160413278373213
42	26.550453071977486	22.332776694938683	25.907273980669515	25.209496252414315
43	24.371355033755833	23.681364838778865	25.32562124013114	26.62165888733416
44	23.531167643273225	23.548668110958136	25.037026851579654	27.883137394188985
45	25.145337152484082	21.431582966913897	27.784714891891703	25.63836498871032
46	24.084795475107487	22.378820079833254	24.578348944437874	28.95803550062139
47	28.96305286028049	20.52219729216124	24.029197771686686	26.48555207587158
48	23.496148197539156	22.0602902687363	25.37387926739021	29.069682266334336
49	27.989282182708703	20.848141739717107	21.529531333082524	29.633044744491666
50	23.747662390182793	26.213281818203406	23.516097554798797	26.522958236815008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16903.0
1	16715.0
2	10903.0
3	5226.0
4	5018.5
5	3823.0
6	2040.0
7	1394.0
8	1553.0
9	1657.0
10	1698.5
11	1686.5
12	1634.5
13	1541.5
14	1552.0
15	1733.5
16	1972.0
17	2340.0
18	2810.0
19	3532.0
20	4282.5
21	5262.0
22	6551.5
23	8346.0
24	10448.0
25	12728.5
26	15567.5
27	19177.5
28	22795.5
29	25913.0
30	29582.0
31	36941.5
32	46905.0
33	55471.5
34	68609.0
35	86227.5
36	96390.5
37	103603.0
38	113621.5
39	127869.5
40	146593.0
41	176849.5
42	221152.0
43	285941.5
44	323934.5
45	291998.0
46	272314.5
47	290117.5
48	321441.5
49	362519.0
50	411435.0
51	465556.0
52	498029.0
53	494175.0
54	473332.5
55	451676.5
56	436570.5
57	436673.0
58	436362.5
59	410584.0
60	381960.5
61	377384.0
62	373003.0
63	360686.5
64	346429.0
65	325819.5
66	284886.5
67	245784.5
68	218911.0
69	192144.0
70	172505.0
71	162400.5
72	154834.5
73	139112.5
74	113745.5
75	90626.0
76	75495.0
77	59712.5
78	47226.0
79	39049.5
80	30897.0
81	23465.5
82	18024.0
83	14602.0
84	11822.5
85	9096.5
86	6677.0
87	4465.0
88	2761.5
89	1885.5
90	1256.5
91	796.0
92	580.0
93	485.5
94	313.0
95	129.5
96	82.0
97	74.0
98	46.5
99	17.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	54722.0
25	52818.0
26	50656.0
27	49355.0
28	47118.0
29	41622.0
30	49278.0
31	40457.0
32	44546.0
33	42525.0
34	42108.0
35	46777.0
36	40960.0
37	41429.0
38	44312.0
39	49961.0
40	50228.0
41	51078.0
42	56897.0
43	59756.0
44	62244.0
45	81260.0
46	273447.0
47	632528.0
48	1043983.0
49	1282837.0
50	1263577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.88035897118348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.597352176147844	11.251756780803115
2	12.425339825997222	4.940404321595345
3	7.119284641148399	4.246018028521901
4	4.766998998162331	3.7907860519495657
5	3.517867868224754	3.4968238016750046
6	2.625804075203996	3.132115656183114
7	2.0158344543154656	2.8052858804789853
8	1.6608071249163254	2.6413953460188573
9	1.3284122224563606	2.3768380659726085
>10	7.239598746352642	24.667681538461963
>50	0.37907415498910346	5.194935619634071
>100	0.2690557340215385	10.432738568584448
>500	0.02924090229040511	4.135982260277006
>1k	0.020634884124454987	8.00930133943543
>5k	0.0032272567588010168	4.348445821591305
>10k+	0.0014669348903640987	4.529490918817277
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	39592	0.7074448059217233	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	22563	0.4031642037788402	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	18427	0.329260594027066	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	16184	0.28918182307125606	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	15598	0.2787109537979147	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	14132	0.25251591223696185	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13397	0.23938265470128628	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	12950	0.2313954899142836	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	12723	0.22733936819918382	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	12594	0.22503434748884074	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	11570	0.20673712882689274	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	11163	0.1994646991438724	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	10939	0.1954621825615713	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	10812	0.19319289860642738	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	10329	0.18456247222584057	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTGC	9961	0.17798690926920302	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	9746	0.1741452080852979	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	9152	0.1635313917911601	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	8574	0.15320346953861524	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	8571	0.15314986440581657	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	8424	0.15052321289868148	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	8368	0.14952258375310618	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	8274	0.1478429562587477	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	7393	0.13210091559353657	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	7119	0.12720498013125753	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6971	0.12456046024652286	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	6830	0.1220410190049851	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	6685	0.1194501042530491	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6588	0.11771687162589192	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTT	6548	0.11700213652190959	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	6483	0.11584069197793827	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	6422	0.1147507209443652	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	6380	0.11400024908518375	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	6366	0.1137500917987899	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	6266	0.11196325403883406	No Hit
CAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	6219	0.1111234402916548	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	6219	0.1111234402916548	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	6136	0.10964036495089144	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	5968	0.10663847751416561	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA	5646	0.10088485992710773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05771485964657421	0.0	0.0	0.0	0.0
2	0.23906102390449424	0.0	0.0	0.0	0.0
3	0.4012880241308866	0.0	0.0	0.0	0.0
4	0.5093023667202182	0.0	0.0	0.0	0.0
5	0.6372578187106572	0.0	0.0	0.0	0.0
6	0.7691443137729991	0.0	0.0	0.0	0.0
7	0.8835376671653731	0.0	0.0	0.0	0.0
8	0.964338470670577	0.0	0.0	0.0	0.0
9	1.0465151392509469	0.0	0.0	0.0	0.0
10	1.116255417022024	0.0	0.0	0.0	0.0
11	1.1803850242268397	0.0	0.0	0.0	0.0
12	1.2339722886479159	0.0	0.0	0.0	0.0
13	1.2758915024964803	0.0	0.0	0.0	0.0
14	1.3197762378809963	0.0	0.0	0.0	0.0
15	1.3665556504366405	4.2884106238940593E-4	0.0	0.0	0.0
16	1.401595538909375	4.2884106238940593E-4	0.0	0.0	0.0
17	1.4382435813660697	4.2884106238940593E-4	0.0	0.0	0.0
18	1.474462782760375	4.2884106238940593E-4	0.0	0.0	0.0
19	1.5461685820674034	4.2884106238940593E-4	0.0	0.0	0.0
20	1.5725423074043519	4.2884106238940593E-4	0.0	0.0	0.0
21	1.6106019516914116	4.2884106238940593E-4	0.0	0.0	0.0
22	1.628363119025373	4.2884106238940593E-4	0.0	0.0	0.0
23	1.6449271050601637	4.2884106238940593E-4	0.0	0.0	0.0
24	1.6602403046629854	4.2884106238940593E-4	0.0	0.0	0.0
25	1.6822898826208408	4.2884106238940593E-4	0.0	0.0	0.0
26	1.7376461164242731	4.2884106238940593E-4	0.0	0.0	0.0
27	1.7516906612175263	4.2884106238940593E-4	0.0	0.0	0.0
28	1.7646273665996066	4.2884106238940593E-4	0.0	0.0	0.0
29	1.7767421266121073	4.2884106238940593E-4	0.0	0.0	0.0
30	1.7886067293382142	4.2884106238940593E-4	0.0	0.0	0.0
31	1.8002033064003278	4.2884106238940593E-4	0.0	0.0	0.0
32	1.813550984467198	4.2884106238940593E-4	0.0	0.0	0.0
33	1.8252011666621102	4.2884106238940593E-4	0.0	0.0	0.0
34	1.8361723505082392	4.2884106238940593E-4	0.0	0.0	0.0
35	1.8463751941175872	4.2884106238940593E-4	0.0	0.0	0.0
36	1.857435719851714	4.2884106238940593E-4	0.0	0.0	0.0
37	1.8665485924274887	4.2884106238940593E-4	0.0	0.0	0.0
38	1.8737138118449117	4.2884106238940593E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	5205	0.0	145.39766	44
ATTAGTC	820	0.0	97.4916	44
ATGTTCT	2105	0.0	95.36629	44
CGTGCGC	2745	0.0	90.92902	44
TTAAGCC	4920	0.0	77.31096	43
TCCTTGT	3015	0.0	75.420815	44
AATGAAG	3630	0.0	71.20736	44
AATCCCG	3280	0.0	68.192	43
TCGTGCG	3935	0.0	65.51744	43
ACGACAC	355	0.0	65.05543	44
CTATACG	125	1.2732926E-11	63.954494	44
CCAGAAT	1780	0.0	62.87661	44
ATCCTTG	2990	0.0	62.655468	43
ATTAAGC	6520	0.0	58.00301	42
CAATGAA	3955	0.0	56.664345	43
CGCTACC	805	0.0	56.27466	44
ACATTGG	665	0.0	56.10043	44
TATTAGT	725	0.0	55.543884	43
AATCCTG	2440	0.0	54.17557	43
TGTGTTA	3015	0.0	52.298016	42
>>END_MODULE