FastQCFastQC Report
Tue 24 May 2016
ERR841223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361993
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC662330.8996612737882257No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG567860.7713400433822742No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC298390.40531144215975207No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC272110.3696145867022693No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT267640.36354286128769747No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC264670.35950862762298197No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG169020.22958457037381047No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG158890.2158247094231141No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG155660.21143731052175682Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC150410.20430608939725967No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG148260.20138568455579897No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG146920.19956552525926063No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT142090.19300480182472327No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG125990.17113572370959876No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA124400.16897598245475104No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA122850.16687057431323285No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT121910.16559374614998956No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG112920.153382378929184No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT111900.1519968845392817No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC93010.1263380717694244No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA92600.125781157357797No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA90120.12241250433136788No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG88740.12053801192150006Illumina Single End Adapter 1 (100% over 31bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG87640.11904385130493875No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG84600.11491453469189661No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA82240.11170888100545599Illumina Single End Adapter 1 (100% over 32bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC81010.11003813777057381No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT80720.10964422269893492No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA79140.1074980647224196No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC78790.10702264998078645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG7250.0174.2255444
TAAGCCA22950.095.6178544
ATGTTCT20950.095.0378744
TCCTTGT32650.082.2921444
CTATGCG2800.080.2840144
AATCCCG76600.072.8398843
ACGACAC4150.069.6439644
CACATGC25500.066.7459244
ATCCTTG45150.064.422543
TCGTGCG32650.063.87337543
AATCGCA2900.062.75072544
TTAAGCC15800.061.0750143
AATCCTG37350.060.97852743
AACGACA7300.058.8902243
TTCAGGA30750.056.74273744
TAATCCC159350.054.786242
CGTGCGC22450.051.9730244
TAATCCT60500.051.8646942
GACCTTA9800.051.80063643
TTTGGAC30150.050.66375443
Produced by FastQC (version 0.11.5)