##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841223.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7361993
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.225358269153475	37.0	34.0	39.0	30.0	39.0
2	36.02154566025803	37.0	35.0	39.0	32.0	39.0
3	36.23934157503274	37.0	35.0	39.0	32.0	39.0
4	36.81096559043183	38.0	35.0	39.0	33.0	41.0
5	37.312157319356324	38.0	35.0	40.0	33.0	41.0
6	37.358637531983526	38.0	35.0	40.0	33.0	41.0
7	37.30285902200668	38.0	35.0	40.0	33.0	41.0
8	37.21259270417671	38.0	35.0	40.0	33.0	41.0
9	37.17153086127629	38.0	35.0	40.0	33.0	41.0
10	37.04847478121753	38.0	35.0	40.0	32.0	41.0
11	36.95289060992044	38.0	35.0	40.0	32.0	41.0
12	36.867756733808356	38.0	35.0	40.0	32.0	41.0
13	36.76301104877443	38.0	35.0	40.0	31.0	41.0
14	36.71443534379889	38.0	35.0	40.0	31.0	41.0
15	36.682123712967396	38.0	35.0	40.0	31.0	41.0
16	36.621834467921936	38.0	35.0	40.0	31.0	41.0
17	36.59085046671465	38.0	35.0	40.0	31.0	41.0
18	36.526312100541254	38.0	35.0	40.0	31.0	41.0
19	36.41756681920236	38.0	35.0	40.0	31.0	41.0
20	36.33095793489616	38.0	34.0	40.0	31.0	41.0
21	36.12444890941896	38.0	34.0	40.0	30.0	41.0
22	36.12744972726814	37.0	34.0	40.0	30.0	41.0
23	36.15693318371805	37.0	34.0	40.0	31.0	41.0
24	36.121151704436556	37.0	34.0	40.0	30.0	41.0
25	36.103123522716125	37.0	34.0	40.0	31.0	41.0
26	36.08711930925999	37.0	34.0	40.0	31.0	41.0
27	36.08200463066613	37.0	34.0	40.0	31.0	41.0
28	36.08919599461594	37.0	34.0	40.0	31.0	41.0
29	36.13936959010929	37.0	34.0	40.0	31.0	41.0
30	36.14235305560449	37.0	34.0	40.0	31.0	41.0
31	36.086386722411	37.0	34.0	40.0	31.0	41.0
32	36.05021784557632	37.0	34.0	40.0	31.0	41.0
33	35.966085372251975	37.0	34.0	40.0	31.0	41.0
34	35.94008249399335	36.0	34.0	40.0	31.0	41.0
35	35.848366503094915	36.0	34.0	40.0	31.0	41.0
36	35.63375923246783	36.0	34.0	39.0	30.0	41.0
37	35.64204100057555	36.0	34.0	39.0	30.0	41.0
38	35.573328304554124	35.0	34.0	39.0	30.0	41.0
39	35.37544190545962	35.0	34.0	39.0	30.0	41.0
40	35.32044698838928	35.0	33.0	39.0	30.0	41.0
41	35.19342028295465	35.0	33.0	39.0	30.0	40.0
42	35.08295358759646	35.0	33.0	39.0	30.0	40.0
43	34.95986727310548	35.0	33.0	38.0	30.0	40.0
44	34.87045285423616	35.0	33.0	38.0	30.0	40.0
45	34.855639294744776	35.0	33.0	38.0	30.0	40.0
46	34.784895042495926	35.0	33.0	38.0	30.0	40.0
47	34.76983866558299	35.0	33.0	38.0	30.0	40.0
48	34.818604147224065	35.0	33.0	38.0	30.0	40.0
49	34.8103632211081	35.0	33.0	38.0	29.0	40.0
50	35.510759451522276	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	73.0
22	287.0
23	897.0
24	2559.0
25	6317.0
26	13785.0
27	28481.0
28	55991.0
29	104819.0
30	182438.0
31	296054.0
32	450674.0
33	664993.0
34	978913.0
35	678560.0
36	807317.0
37	918807.0
38	933851.0
39	844424.0
40	392739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.02653398339281	17.621736396652373	33.35172961995481
2	27.257999294484524	21.559678201269683	29.54614327940817	21.636179224837623
3	32.17566221538108	16.125416038836224	24.881509667287105	26.81741207849559
4	20.515164847345005	18.08515710351803	28.110390759676086	33.28928728946089
5	25.874746145507068	20.058956861273845	27.025358486485928	27.040938506733163
6	27.0423375844014	19.341542432871098	24.342470849945116	29.27364913278239
7	29.510758839352334	21.669118674793634	22.427459520811823	26.392662965042213
8	28.468282977177513	17.123012749400875	28.08830434910764	26.320399924313975
9	26.90175065366131	18.344380387213082	23.368563376792125	31.38530558233348
10	27.48968927299985	22.060181257982723	24.997279948513942	25.452849520503484
11	32.921533611890155	18.30649662394409	24.121158496075722	24.650811268090038
12	28.35009487240751	17.233431218964757	22.763075161848157	31.653398746779576
13	25.99084242541388	18.785972765798608	27.370428089241592	27.85275671954592
14	27.277776547736458	19.76875011970264	21.36157967007032	31.591893662490577
15	24.88119725188546	20.633135619661687	22.439942553599277	32.04572457485357
16	26.397865360643515	20.638664013942964	27.928619329032234	25.034851296381294
17	27.656627763704744	20.070366815073037	19.406579169526513	32.8664262516957
18	26.71070184391645	19.40479976006497	20.441801561071845	33.44269683494673
19	26.298653095703838	18.63997969028224	28.345435807939506	26.715931406074418
20	28.25876090890062	17.763654488669033	25.7513692284141	28.22621537401625
21	28.447174562649003	21.13833849067773	21.008360100315226	29.406126846358045
22	28.10699494009299	19.41868187052066	27.089960558234704	25.384362631151646
23	27.733807951189306	20.506430799377288	23.752290989681736	28.00747025975167
24	31.511045989856278	22.698812672057688	20.85924287078241	24.93089846730362
25	27.911346157672547	20.94119406733087	23.266384857793096	27.88107491720349
26	31.016509009839016	21.400754059505143	22.11598008201166	25.466756848644177
27	28.824463297069524	22.066912664104144	24.549774207130923	24.558849831695408
28	29.193648935135712	20.01328654579871	25.415451544261035	25.377612974804535
29	30.85191422275314	19.497082052598362	26.227103192110455	23.423900532538035
30	27.402975756348113	20.803166726249124	23.851899628331715	27.941957889071045
31	26.658735334447176	20.337623862484435	29.7201301035921	23.283510699476288
32	28.28058773355085	19.32455811444622	25.12418403839871	27.270670113604222
33	25.60934113514567	19.346231057105385	25.80787335308165	29.236554454667296
34	24.71234077637985	18.95604022868116	32.16998861663403	24.16163037830496
35	30.59761274068119	19.805445215507476	23.072619684764945	26.524322359046394
36	26.24733115053682	20.932307926597122	22.212091677453742	30.608269245412323
37	24.382522285493387	21.234666575708605	26.653877225668488	27.728933913129524
38	32.08673496063263	21.106925116130203	21.539454946322024	25.266884976915144
39	26.272733236469602	21.04220163789475	26.10308046124211	26.581984664393538
40	26.8174131880413	20.543244044300472	21.96477805447308	30.67456471318515
41	26.178408380521645	21.620875239129678	22.985495733240185	29.21522064710849
42	25.341803708175625	21.533308165211363	28.494241164850116	24.6306469617629
43	23.79158099680001	26.150695891505606	23.407727248901992	26.64999586279239
44	22.962977559687864	25.341374100056246	23.89446694071894	27.80118139953695
45	24.737282798245612	19.979810969880806	29.497327340039426	25.785578891834156
46	24.486374959607176	22.169565144630027	22.67066905696712	30.673390838795672
47	25.510470589236306	21.707638108675717	24.140509769553443	28.64138153253453
48	23.49976203580364	21.487679481282367	25.88667143481332	29.125887048100672
49	28.305715273117034	21.31447900910557	21.849769355598074	28.530036362179327
50	24.083168858806577	24.80210000490313	24.316616574202342	26.798114562087942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19727.0
1	19442.0
2	12001.0
3	4784.0
4	4605.5
5	3720.5
6	2474.5
7	2198.0
8	2490.0
9	2635.0
10	2737.0
11	2809.0
12	2799.0
13	2773.0
14	2853.0
15	3177.5
16	3598.5
17	4210.5
18	4905.0
19	5779.5
20	7000.0
21	8944.5
22	11177.5
23	13938.5
24	16793.5
25	19587.0
26	23581.5
27	28909.0
28	34358.0
29	39679.5
30	46289.5
31	55981.5
32	67179.0
33	76515.5
34	89966.0
35	108851.0
36	122615.0
37	133629.5
38	147721.5
39	167677.5
40	193606.5
41	231544.0
42	283082.0
43	329198.0
44	350178.5
45	348947.0
46	357393.5
47	380066.0
48	421250.0
49	474499.5
50	539497.5
51	605526.0
52	654825.0
53	667927.0
54	635106.5
55	618715.0
56	644168.0
57	682997.0
58	707015.5
59	650170.5
60	548467.0
61	493715.5
62	467179.0
63	442705.0
64	424857.0
65	410920.0
66	366975.5
67	318850.0
68	285288.5
69	252593.5
70	222412.5
71	194797.5
72	172548.5
73	152491.5
74	131893.5
75	112928.0
76	98472.5
77	80281.5
78	63984.5
79	51809.0
80	39717.5
81	29662.5
82	22211.5
83	16516.0
84	12287.5
85	9408.0
86	7033.0
87	4968.5
88	3390.0
89	2388.5
90	1638.0
91	1008.5
92	692.0
93	576.5
94	393.0
95	183.5
96	118.0
97	109.0
98	61.5
99	13.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	74548.0
25	69447.0
26	66975.0
27	65660.0
28	61076.0
29	53555.0
30	67310.0
31	62567.0
32	72684.0
33	61016.0
34	64810.0
35	75845.0
36	58006.0
37	62927.0
38	73729.0
39	76104.0
40	74737.0
41	75657.0
42	78446.0
43	78742.0
44	79993.0
45	111956.0
46	389917.0
47	893002.0
48	1318756.0
49	1828055.0
50	1366473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.809557896701165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.64152212082693	11.022339539130995
2	12.961113379053382	5.13507851776132
3	7.1933885666673625	4.27493541854596
4	4.886566431343407	3.872028825510945
5	3.5681926593346263	3.5342159535836695
6	2.7049045101219726	3.2149777498985586
7	2.0916401512597105	2.9004126672810817
8	1.6870591590162534	2.673591686855396
9	1.3483857027111021	2.4039832180445697
>10	7.2306406372577845	24.470852942166257
>50	0.38445262203123537	5.243211107827398
>100	0.24787983877095576	9.724782787560605
>500	0.02860544269291132	3.8445828386075562
>1k	0.02187907365432426	8.417998492317205
>5k	0.002365305259920487	3.2281640365607474
>10k+	0.0014043999980777892	6.038844218347673
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	66233	0.8996612737882257	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	56786	0.7713400433822742	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	29839	0.40531144215975207	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	27211	0.3696145867022693	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	26764	0.36354286128769747	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	26467	0.35950862762298197	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	16902	0.22958457037381047	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	15889	0.2158247094231141	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	15566	0.21143731052175682	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	15041	0.20430608939725967	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	14826	0.20138568455579897	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	14692	0.19956552525926063	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	14209	0.19300480182472327	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	12599	0.17113572370959876	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA	12440	0.16897598245475104	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA	12285	0.16687057431323285	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT	12191	0.16559374614998956	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	11292	0.153382378929184	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	11190	0.1519968845392817	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	9301	0.1263380717694244	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	9260	0.125781157357797	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	9012	0.12241250433136788	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	8874	0.12053801192150006	Illumina Single End Adapter 1 (100% over 31bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	8764	0.11904385130493875	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	8460	0.11491453469189661	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	8224	0.11170888100545599	Illumina Single End Adapter 1 (100% over 32bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	8101	0.11003813777057381	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	8072	0.10964422269893492	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	7914	0.1074980647224196	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	7879	0.10702264998078645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.2310651477120394	0.0	0.0	0.0	0.0
2	0.5341488371423336	0.0	0.0	0.0	0.0
3	0.6820299883469055	0.0	0.0	0.0	0.0
4	0.7943229503206537	0.0	0.0	0.0	0.0
5	0.9930327290449746	0.0	0.0	0.0	0.0
6	1.1014408734156633	0.0	0.0	0.0	0.0
7	1.1947444122807507	0.0	0.0	0.0	0.0
8	1.265160127155785	0.0	0.0	0.0	0.0
9	1.3267467111147755	0.0	0.0	0.0	0.0
10	1.3808896585476242	0.0	0.0	0.0	0.0
11	1.440099168798449	0.0	0.0	0.0	0.0
12	1.4824110808037987	0.0	0.0	0.0	0.0
13	1.5164779428613964	0.0	0.0	0.0	0.0
14	1.5587083551967518	0.0	0.0	0.0	0.0
15	1.6139651314528551	0.0	0.0	0.0	0.0
16	1.645030088998998	0.0	0.0	0.0	0.0
17	1.6809578601881312	0.0	0.0	0.0	0.0
18	1.7142912252157805	0.0	0.0	0.0	0.0
19	1.8248319442846523	0.0	0.0	0.0	0.0
20	1.8520120842277357	0.0	0.0	0.0	0.0
21	1.912620672146795	0.0	0.0	0.0	0.0
22	1.9299257687422413	0.0	0.0	0.0	0.0
23	1.9486027764492577	0.0	0.0	0.0	0.0
24	1.9646310448814608	0.0	0.0	0.0	0.0
25	1.988157282953135	0.0	0.0	0.0	0.0
26	2.084204644041362	0.0	0.0	0.0	0.0
27	2.098847418083663	0.0	0.0	0.0	0.0
28	2.113544525239293	0.0	0.0	0.0	0.0
29	2.1268289714483566	0.0	0.0	0.0	0.0
30	2.139936835039099	0.0	0.0	0.0	0.0
31	2.152786616341526	0.0	0.0	0.0	0.0
32	2.165296815685644	0.0	0.0	0.0	0.0
33	2.177671182246438	0.0	0.0	0.0	0.0
34	2.1880080570573757	0.0	0.0	0.0	0.0
35	2.19929576135158	0.0	0.0	0.0	0.0
36	2.21044763286246	0.0	0.0	0.0	0.0
37	2.220431342436756	0.0	0.0	0.0	0.0
38	2.227481063891259	0.0	0.0	2.716655666475097E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	725	0.0	174.22554	44
TAAGCCA	2295	0.0	95.61785	44
ATGTTCT	2095	0.0	95.03787	44
TCCTTGT	3265	0.0	82.29214	44
CTATGCG	280	0.0	80.28401	44
AATCCCG	7660	0.0	72.83988	43
ACGACAC	415	0.0	69.64396	44
CACATGC	2550	0.0	66.74592	44
ATCCTTG	4515	0.0	64.4225	43
TCGTGCG	3265	0.0	63.873375	43
AATCGCA	290	0.0	62.750725	44
TTAAGCC	1580	0.0	61.07501	43
AATCCTG	3735	0.0	60.978527	43
AACGACA	730	0.0	58.89022	43
TTCAGGA	3075	0.0	56.742737	44
TAATCCC	15935	0.0	54.7862	42
CGTGCGC	2245	0.0	51.97302	44
TAATCCT	6050	0.0	51.86469	42
GACCTTA	980	0.0	51.800636	43
TTTGGAC	3015	0.0	50.663754	43
>>END_MODULE