FastQCFastQC Report
Tue 24 May 2016
ERR841222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11737872
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGCAG585890.49914498982439065No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA396120.3374717325252823No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC333910.2844723472874811No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG314250.2677231443655204No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG288950.2461689819074531No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG281170.23954086396580232No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC268960.22913863773603937No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA264580.2254071266069352No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC256910.21887272241510217No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC244830.2085812487987601No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG240340.20475602391983827No Hit
AAGCAGTGGTATCAACGCAGAGTG212190.18077382339831274No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT207220.17653966579291375No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC200420.17074645216782053No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT176790.15061503482062166Illumina Single End Adapter 2 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGT171580.14617641085198407No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCGTC154420.13155706588042534Illumina Single End Adapter 2 (100% over 25bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG153190.1305091757688276No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC150270.12802150168275817No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT143470.12222828805766497No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC142030.12100148987823348No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC137030.11674177397742963No Hit
AAGCAGTGGTATCAACGCAGAGCG136670.11643507443257176No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG134540.11462043545882933No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG134140.11427965818676504No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT133500.11373441455146213No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG132020.1124735386448242Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG131400.11194533387312454No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG130410.11110191012476536No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTATGCCG127390.10852904172067987Illumina Single End Adapter 2 (100% over 23bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA119670.10195204036983875No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA118560.1010063834398603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA60850.0113.7781444
CGTGCGC63600.095.9209344
CTATACG3500.083.1140544
ATGTTCT34450.073.8255444
TTAAGCC51100.073.0743
CTATCAT28750.067.9367144
TCGTGCG90850.067.6244743
AATCCCG50750.067.39928443
ATTAAGC67650.057.00452442
ACGACAC7350.056.5401744
ACTTCTA22200.055.03497744
GTGTTAG45650.053.36886243
TTCAGGA41950.053.29601344
TGTGTTA49850.050.48503542
CCCGAGG42100.050.14481744
CGCTACC16700.048.7735144
TTTGGAC39150.047.5740743
AATCCTG33800.047.4001343
AACGACA10750.047.21489343
CTAATCC142200.045.9192241
Produced by FastQC (version 0.11.5)