FastQCFastQC Report
Tue 24 May 2016
ERR841221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16352145
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1182320.7230366413702911Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA682850.4175904751333846Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAG632990.38709906253889015No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA560940.343037564796545Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA532690.32576154382192674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA514910.314888352567813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA489930.29961206924229206No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA484920.29654825100927124Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAA437310.2674328046870915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA416020.25441310604816675No Hit
AAAAAAAAAAAAAAAAAAAAAAAA381700.23342503384112603No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG339330.20751406008202594No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA326100.19942337840081528No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG305450.18679506572379342No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG294130.17987242652263663Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA285170.17439302305599663Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA279410.17087054939887092Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA278180.17011835450333887Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT275010.16817977091078878Illumina Single End Adapter 2 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274220.16769665386406493No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG258040.1578019275147083No Hit
AAGCAGTGGTATCAACGCAGAGTG251920.15405929925401224No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT240340.14697765950583241No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG230360.14087448466240973Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGC224220.1371196255904042No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA216530.13241687864191518Illumina Single End Adapter 1 (100% over 32bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG214260.13102868155829098Illumina Single End Adapter 2 (100% over 34bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT208910.1277569395330093No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT205900.1259162024309349No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA205870.1258978562139707Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG205020.12537804673331845No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG204620.1251334305071292Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGCG203680.12455858237558437No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC202630.1239164647818375No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAG201460.12320096232023384No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG191290.11698159476937124No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA188890.11551389741223553No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA188800.11545885876134293No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG188410.11522035794080838No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT187340.11456600953575205No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG187000.11435808574349114No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA182890.11184465401939624Illumina Single End Adapter 1 (100% over 32bp)
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC179370.10969203122893052No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC176770.10810202575870016No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG175890.10756387006108373No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG167500.10243304471676347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCT42450.0115.4240944
CTATACG6100.095.8177444
AATCGCA3150.090.8019644
ACGACAC9450.085.5380744
ATCCTTG71100.073.2153443
TCGTGCG59450.071.3632843
TTCAGGA49000.070.3010244
CGTGCGC43700.069.7208344
AACGACA19750.069.0377143
CGCTACC17500.068.219944
TCCTTGT59950.067.8322944
GACCGTA2900.064.3237244
CATACAA10700.060.43624544
ACGCTAC31700.058.84231643
GTTCAGG89100.057.9123643
CTAAAAC34100.057.67379843
ACTTCTA18000.057.3434144
AATCCCG23050.055.8552543
CCTTAAC21350.055.55449343
TGTGTTA56650.055.1604442
Produced by FastQC (version 0.11.5)