##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841221.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16352145
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.20195619596083	38.0	35.0	39.0	31.0	39.0
2	36.907701099763976	38.0	35.0	39.0	33.0	39.0
3	37.08301039404922	39.0	37.0	39.0	33.0	39.0
4	37.53358363688678	39.0	37.0	40.0	33.0	41.0
5	38.11929737658271	40.0	37.0	41.0	33.0	41.0
6	38.10409845313872	40.0	37.0	41.0	33.0	41.0
7	37.85276708346214	39.0	37.0	41.0	33.0	41.0
8	37.90091061447902	39.0	37.0	40.0	33.0	41.0
9	37.89377460877457	39.0	37.0	40.0	33.0	41.0
10	37.51942665625825	39.0	36.0	40.0	32.0	41.0
11	37.60562152549406	39.0	36.0	40.0	32.0	41.0
12	37.552875173257085	39.0	36.0	40.0	32.0	41.0
13	37.49559565427043	39.0	36.0	40.0	32.0	41.0
14	37.412397884191954	39.0	36.0	40.0	32.0	41.0
15	37.23217253760898	39.0	36.0	40.0	32.0	41.0
16	37.13302242610985	38.0	35.0	40.0	32.0	41.0
17	36.93344866988398	38.0	35.0	40.0	31.0	41.0
18	36.91488939218677	38.0	35.0	40.0	31.0	41.0
19	36.858519111712866	38.0	35.0	40.0	31.0	41.0
20	36.688955363348356	38.0	35.0	40.0	31.0	41.0
21	36.503593626401916	38.0	35.0	40.0	30.0	41.0
22	36.32571359904159	38.0	35.0	40.0	30.0	41.0
23	36.238582216583815	38.0	34.0	40.0	30.0	41.0
24	36.29745828452475	38.0	34.0	40.0	30.0	41.0
25	36.30917203853098	38.0	35.0	40.0	30.0	41.0
26	36.37222047456139	38.0	35.0	40.0	30.0	41.0
27	36.410688308209515	38.0	35.0	40.0	30.0	41.0
28	36.28128849695604	38.0	34.0	40.0	30.0	41.0
29	36.368237382117854	38.0	35.0	40.0	30.0	41.0
30	36.48703536215144	38.0	35.0	40.0	31.0	41.0
31	36.4570108332807	38.0	35.0	40.0	31.0	41.0
32	36.37932374586976	38.0	34.0	40.0	30.0	41.0
33	36.21979720711342	38.0	34.0	40.0	30.0	41.0
34	36.25509012793691	38.0	34.0	40.0	30.0	41.0
35	36.13403319986477	37.0	34.0	40.0	30.0	41.0
36	36.02071149168801	37.0	34.0	40.0	30.0	41.0
37	35.91139604723315	37.0	34.0	40.0	30.0	41.0
38	35.86387716295772	37.0	34.0	40.0	30.0	41.0
39	35.659151298441394	36.0	34.0	40.0	30.0	41.0
40	35.52583094563157	36.0	34.0	39.0	29.0	41.0
41	35.3962883763432	36.0	33.0	39.0	29.0	41.0
42	35.258866940106	36.0	33.0	39.0	29.0	41.0
43	35.13426785146024	35.0	33.0	39.0	29.0	40.0
44	35.06666959502902	35.0	33.0	39.0	29.0	40.0
45	35.028079713724935	35.0	33.0	39.0	29.0	40.0
46	34.94952962919226	35.0	33.0	39.0	29.0	40.0
47	34.90274498466395	35.0	33.0	39.0	29.0	40.0
48	34.90759503876646	35.0	33.0	38.0	29.0	40.0
49	34.906923646774054	35.0	33.0	38.0	29.0	40.0
50	35.22734314158573	36.0	33.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	21.0
20	92.0
21	328.0
22	712.0
23	1589.0
24	3691.0
25	7997.0
26	17197.0
27	36920.0
28	78163.0
29	160290.0
30	304104.0
31	517627.0
32	801035.0
33	1150792.0
34	1555423.0
35	1784787.0
36	2192197.0
37	2428964.0
38	2344506.0
39	2016580.0
40	949126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.98137063975399	17.09041229759154	28.928217062654472
2	24.192679309044777	30.81993830167235	26.761180261060552	18.226202128222322
3	30.102222063221674	19.801536740286977	23.21021492898944	26.88602626750191
4	21.741166067204027	21.732329306032938	27.22983437341095	29.29667025335208
5	23.019322541476974	27.287074570339243	24.260211733689985	25.4333911544938
6	29.023391120859067	23.80355604723417	23.357987591230387	23.815065240676375
7	28.713541862550755	21.8810865485843	25.450838406826747	23.954533182038197
8	32.92577823887937	19.72679425237484	22.1101512981936	25.237276210552196
9	31.346719344770978	23.58673433974564	21.09419895677295	23.972347358710433
10	28.08076860864431	20.365419949492864	26.952971613204262	24.600839828658565
11	28.120579899456615	25.989299874725912	20.898768938264674	24.991351287552796
12	27.42108145445139	20.480163305792605	26.952714766166764	25.14604047358924
13	26.061767431734488	21.35061180047021	19.628739838106863	32.958880929688426
14	28.698864888979397	26.44140569937461	19.047042452228744	25.812686959417253
15	23.846663541694376	27.105612138346373	22.87880275034254	26.16892156961671
16	26.37886344574366	24.627515228124505	19.886724341057395	29.10689698507444
17	30.993554668210194	20.49144011381993	21.575891113979235	26.93911410399064
18	28.799004656575633	21.175778468207078	19.335958676980912	30.68925819823638
19	25.909995294195348	24.31518311512037	21.674826146661495	28.099995444022785
20	31.494412506738414	19.63932560529521	21.56864435827838	27.297617529688
21	31.55121850986522	25.89339196784275	19.67546153730902	22.87992798498301
22	30.916286517762654	20.921989133535693	23.91768174756278	24.244042601138872
23	27.41958318006598	22.660929193081397	23.26581008179661	26.653677545056016
24	33.05937539081265	21.371820027280826	22.937131489477373	22.631673092429157
25	28.45457465354298	22.352979526955767	22.978262231417933	26.214183588083323
26	27.110920436787918	25.30506295273617	21.078065409559247	26.505951200916666
27	30.67415702962682	21.834824340593613	24.31065250710925	23.180366122670314
28	31.607237055420985	21.58184261797721	22.754677898622386	24.05624242797942
29	29.00200014206915	20.85098888375418	23.69040312254156	26.456607851635113
30	28.158734474681975	20.82680024869521	26.237074815359325	24.777390461263494
31	27.5558142552224	19.97027770086357	28.42398652837545	24.049921515538582
32	30.103227556622	19.484688838241045	24.862024104389832	25.550059500747125
33	25.781554027300096	22.22728874099127	26.923384668570886	25.06777256313775
34	26.522974521322556	21.309624038405342	28.758643061433354	23.40875837883875
35	28.12361284430194	21.844078719186307	24.773591014671716	25.25871742184004
36	27.500236163383835	23.71001034669971	22.98928567138544	25.80046781853102
37	25.127953860534625	24.7672802677258	26.983708526566573	23.121057345173003
38	27.796249690270635	24.295037629395814	22.7256199343538	25.183092745979746
39	27.304876001830014	24.410001242929575	23.72887894752644	24.556243807713972
40	27.267299955709163	23.760086967628293	22.776577837171132	26.19603523949141
41	25.976217838258858	24.472277654087435	23.594515779868516	25.95698872778519
42	26.20287030237418	24.597471615352813	24.285116834849006	24.914541247423998
43	24.689576807212514	25.048964639665254	24.15556524280188	26.105893310320354
44	23.69663544084988	24.099543649178745	25.319266858646657	26.884554051324717
45	25.833535121665406	22.218735390107565	25.57523727365563	26.37249221457139
46	25.66803860962816	22.029465280411582	23.82995042352215	28.47254568643811
47	26.223289749964874	22.044221034484718	25.490766731438047	26.241722484112362
48	25.674820375900737	22.443073630138976	25.00889320234051	26.873212791619782
49	27.398932567730334	21.481443104302492	22.978093346803725	28.14153098116345
50	23.396369189264448	24.06624803251175	26.056826557309826	26.480556220913975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434323.0
1	427897.0
2	262737.5
3	103558.5
4	101305.0
5	76223.0
6	34894.5
7	17050.5
8	16089.0
9	13346.0
10	10545.5
11	9162.0
12	8841.0
13	8827.0
14	9401.5
15	10356.5
16	11334.0
17	12494.5
18	13909.0
19	15896.5
20	18340.5
21	21694.0
22	25979.0
23	31488.5
24	37093.0
25	42615.0
26	49531.5
27	58492.5
28	68010.0
29	75710.5
30	83586.0
31	96568.5
32	114578.5
33	130279.0
34	148903.0
35	175678.0
36	207579.5
37	241894.5
38	283088.5
39	342105.5
40	424582.0
41	558677.5
42	735266.5
43	872994.5
44	908604.5
45	878465.5
46	879969.5
47	944176.0
48	1109546.5
49	1311089.5
50	1549497.5
51	1805709.0
52	2023428.0
53	2060988.5
54	1837381.0
55	1519855.0
56	1250205.0
57	1127426.5
58	1067877.5
59	1002498.0
60	957500.5
61	931109.0
62	883064.5
63	820308.5
64	759572.5
65	709195.0
66	663341.0
67	622488.5
68	563897.0
69	492844.5
70	437346.5
71	391168.5
72	352312.5
73	317724.5
74	278058.0
75	238743.0
76	209229.5
77	171384.5
78	137756.0
79	115639.5
80	91832.0
81	66751.5
82	47648.5
83	34927.5
84	25893.0
85	19698.5
86	14466.5
87	10271.0
88	7384.0
89	5357.0
90	3633.0
91	2236.0
92	1553.5
93	1281.0
94	869.0
95	445.5
96	313.0
97	291.0
98	153.5
99	15.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	277496.0
25	240786.0
26	236577.0
27	287634.0
28	274720.0
29	213577.0
30	223561.0
31	213941.0
32	273792.0
33	182415.0
34	223795.0
35	217425.0
36	210156.0
37	188231.0
38	231317.0
39	267210.0
40	254048.0
41	246006.0
42	245707.0
43	233166.0
44	224892.0
45	266912.0
46	735080.0
47	1776003.0
48	2469791.0
49	3645516.0
50	2492391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.020695408952193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.65915371953886	9.813988929982239
2	12.9315694966336	4.4020861112379555
3	6.964370900026395	3.5561530741295857
4	4.58724751109283	3.123125706071404
5	3.207878144336961	2.73001584018971
6	2.423933446984222	2.4754219735614007
7	1.8405363525953602	2.1929046052640624
8	1.444464838716898	1.966863683899326
9	1.1726843333827024	1.7963912564421411
>10	6.943378460049203	20.91784510493685
>50	0.4412219635207732	5.204425043283745
>100	0.3119761232997743	10.4886707959493
>500	0.035529821074174635	4.220149831991849
>1k	0.028851463234320144	10.111927915692547
>5k	0.003846855510223827	4.418523578530163
>10k+	0.0033565700040188292	12.58150654883782
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	118232	0.7230366413702911	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	68285	0.4175904751333846	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAG	63299	0.38709906253889015	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	56094	0.343037564796545	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA	53269	0.32576154382192674	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	51491	0.314888352567813	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	48993	0.29961206924229206	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	48492	0.29654825100927124	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAA	43731	0.2674328046870915	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41602	0.25441310604816675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	38170	0.23342503384112603	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	33933	0.20751406008202594	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGA	32610	0.19942337840081528	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	30545	0.18679506572379342	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	29413	0.17987242652263663	Illumina Single End Adapter 1 (100% over 31bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	28517	0.17439302305599663	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	27941	0.17087054939887092	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	27818	0.17011835450333887	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCT	27501	0.16817977091078878	Illumina Single End Adapter 2 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27422	0.16769665386406493	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	25804	0.1578019275147083	No Hit
AAGCAGTGGTATCAACGCAGAGTG	25192	0.15405929925401224	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGT	24034	0.14697765950583241	No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	23036	0.14087448466240973	Illumina Single End Adapter 2 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	22422	0.1371196255904042	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	21653	0.13241687864191518	Illumina Single End Adapter 1 (100% over 32bp)
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	21426	0.13102868155829098	Illumina Single End Adapter 2 (100% over 34bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	20891	0.1277569395330093	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	20590	0.1259162024309349	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA	20587	0.1258978562139707	Illumina Single End Adapter 1 (100% over 32bp)
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	20502	0.12537804673331845	No Hit
AAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTG	20462	0.1251334305071292	Illumina Single End Adapter 2 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGCG	20368	0.12455858237558437	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	20263	0.1239164647818375	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAG	20146	0.12320096232023384	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	19129	0.11698159476937124	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	18889	0.11551389741223553	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	18880	0.11545885876134293	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	18841	0.11522035794080838	No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	18734	0.11456600953575205	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	18700	0.11435808574349114	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA	18289	0.11184465401939624	Illumina Single End Adapter 1 (100% over 32bp)
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTC	17937	0.10969203122893052	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	17677	0.10810202575870016	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	17589	0.10756387006108373	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	16750	0.10243304471676347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.6926858831058555	0.0	0.0	0.0	0.0
2	1.717340446773191	0.0	0.0	0.0	0.0
3	2.329058359010393	0.0	0.0	0.0	0.0
4	2.7846193878540095	0.0	0.0	0.0	0.0
5	3.472235599672092	0.0	0.0	0.0	0.0
6	3.78095350793428	0.0	0.0	0.0	0.0
7	3.9895866872511223	0.0	0.0	0.0	0.0
8	4.161619163724392	0.0	0.0	0.0	0.0
9	4.2898714511154346	0.0	0.0	0.0	0.0
10	4.398248670128598	0.0	0.0	0.0	0.0
11	4.545629946407643	0.0	0.0	0.0	0.0
12	4.6320956669599	0.0	0.0	0.0	0.0
13	4.706165460250016	0.0	0.0	0.0	0.0
14	4.806317458657564	0.0	0.0	0.0	0.0
15	4.979842094110589	0.0	0.0	0.0	0.0
16	5.0535388476557666	0.0	0.0	0.0	0.0
17	5.145661318438651	0.0	0.0	0.0	0.0
18	5.2392881790126005	0.0	0.0	0.0	0.0
19	5.735241462205723	0.0	0.0	0.0	0.0
20	5.8135247699919494	0.0	0.0	0.0	0.0
21	6.0289093571516155	0.0	0.0	0.0	0.0
22	6.068610570662137	0.0	0.0	0.0	0.0
23	6.109192402586939	0.0	0.0	0.0	0.0
24	6.140760126576666	0.0	0.0	0.0	0.0
25	6.201015218492742	0.0	0.0	0.0	0.0
26	6.556577134070179	0.0	0.0	0.0	0.0
27	6.595899192430107	0.0	0.0	0.0	0.0
28	6.623394056253782	0.0	0.0	0.0	0.0
29	6.647464292910808	0.0	0.0	0.0	0.0
30	6.667541169675293	0.0	0.0	0.0	0.0
31	6.687526315354958	0.0	0.0	0.0	0.0
32	6.707590961308134	0.0	0.0	0.0	0.0
33	6.726762758035719	0.0	0.0	0.0	0.0
34	6.745261860141285	0.0	0.0	0.0	0.0
35	6.765577237726305	0.0	0.0	6.115405654732147E-6	0.0
36	6.782076602182772	0.0	0.0	6.115405654732147E-6	0.0
37	6.796374420603535	0.0	0.0	6.115405654732147E-6	0.0
38	6.805896107207953	6.726946220205362E-5	0.0	6.115405654732147E-6	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTTCT	4245	0.0	115.42409	44
CTATACG	610	0.0	95.81774	44
AATCGCA	315	0.0	90.80196	44
ACGACAC	945	0.0	85.53807	44
ATCCTTG	7110	0.0	73.21534	43
TCGTGCG	5945	0.0	71.36328	43
TTCAGGA	4900	0.0	70.30102	44
CGTGCGC	4370	0.0	69.72083	44
AACGACA	1975	0.0	69.03771	43
CGCTACC	1750	0.0	68.2199	44
TCCTTGT	5995	0.0	67.83229	44
GACCGTA	290	0.0	64.32372	44
CATACAA	1070	0.0	60.436245	44
ACGCTAC	3170	0.0	58.842316	43
GTTCAGG	8910	0.0	57.91236	43
CTAAAAC	3410	0.0	57.673798	43
ACTTCTA	1800	0.0	57.34341	44
AATCCCG	2305	0.0	55.85525	43
CCTTAAC	2135	0.0	55.554493	43
TGTGTTA	5665	0.0	55.16044	42
>>END_MODULE