FastQCFastQC Report
Tue 24 May 2016
ERR841220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844966
Sequences flagged as poor quality0
Sequence length24-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG61090.33111721300013114No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG46370.2513325448815859Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA40040.21702296952897776No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG37540.20347258431862703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA36440.19751041482607268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA35790.1939873146713815No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT33750.18293020033973525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32060.17377013993753815No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG30390.16471848261702385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA29860.16184580095242948No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA29510.15994874702298037No Hit
AAAAAAAAAAAAAAAAAAAAAAAA29040.15740127460343442No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA25470.13805132452305355Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG25310.1371840998695911No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA24210.13122193037703678No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT22230.12049002529043895No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC21130.11452785579788463No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG20900.11328122035853234No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20580.11154677105160746Illumina Single End Adapter 1 (100% over 32bp)
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT19930.10802367089691625No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG19850.10759005857018504No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG19660.10656022929419837No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG18810.10195309832267913Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG452.8079023E-586.2906544
CGTGCGC6000.082.51543444
ACTTCTA3650.074.4700144
TCGTGCG7500.073.4655343
ACGACAC2200.070.6014444
ACATTGG2350.066.0949644
ATGTTCT5050.065.3587644
CGCTACC2800.062.40663544
CGGGTAG500.004606855658.2461944
GGTCGAG1003.6464735E-758.2461944
AACGACA3600.058.1855143
GTTCAGG16100.057.41557343
TTCAGGA9850.057.1620844
AGGTCGA853.6379788E-1253.57233443
CGGGATA550.00671722652.9510844
ACATTTG2350.049.57122444
TACGCTA4200.049.34963242
CTCGTGC9700.049.18004642
AATCCCG2500.049.179443
AAACCGT1002.484487E-548.5384944
Produced by FastQC (version 0.11.5)