##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841220.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1844966
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.197216642474714	38.0	35.0	39.0	31.0	39.0
2	36.90383129011592	38.0	35.0	39.0	33.0	39.0
3	36.96801404470326	38.0	36.0	39.0	33.0	39.0
4	37.454773692306524	39.0	37.0	40.0	33.0	41.0
5	38.066513962858934	39.0	37.0	40.0	33.0	41.0
6	38.03983759050302	39.0	37.0	41.0	33.0	41.0
7	38.016432281136886	39.0	37.0	41.0	33.0	41.0
8	37.91783751028474	39.0	37.0	40.0	33.0	41.0
9	37.9073023567914	39.0	37.0	40.0	33.0	41.0
10	37.74144401577048	39.0	36.0	40.0	33.0	41.0
11	37.64991820987487	39.0	36.0	40.0	33.0	41.0
12	37.6348014001342	39.0	36.0	40.0	33.0	41.0
13	37.44562935035117	39.0	36.0	40.0	32.0	41.0
14	37.40248817593387	39.0	36.0	40.0	32.0	41.0
15	37.30027003207647	39.0	36.0	40.0	32.0	41.0
16	37.21561101939006	38.0	36.0	40.0	32.0	41.0
17	37.10239321483431	38.0	35.0	40.0	31.0	41.0
18	37.069880962576	38.0	35.0	40.0	31.0	41.0
19	36.98709136103321	38.0	35.0	40.0	31.0	41.0
20	36.86788266016826	38.0	35.0	40.0	31.0	41.0
21	36.72499222207889	38.0	35.0	40.0	31.0	41.0
22	36.70665638282765	38.0	35.0	40.0	31.0	41.0
23	36.70252947750799	38.0	35.0	40.0	31.0	41.0
24	36.66544803535675	38.0	35.0	40.0	31.0	41.0
25	36.65798636648042	38.0	35.0	40.0	31.0	41.0
26	36.67132926538113	38.0	35.0	40.0	31.0	41.0
27	36.72512585355009	38.0	35.0	40.0	31.0	41.0
28	36.697339625299975	38.0	35.0	40.0	31.0	41.0
29	36.72866302445417	38.0	35.0	40.0	31.0	41.0
30	36.76178271997381	38.0	35.0	40.0	31.0	41.0
31	36.6892980936892	38.0	35.0	40.0	31.0	41.0
32	36.63508725787945	38.0	35.0	40.0	31.0	41.0
33	36.53716115217994	38.0	35.0	40.0	31.0	41.0
34	36.48837030661503	38.0	35.0	40.0	31.0	41.0
35	36.37974488504419	38.0	34.0	40.0	31.0	41.0
36	36.285169155880894	37.0	34.0	40.0	31.0	41.0
37	36.1806926282979	37.0	34.0	40.0	31.0	41.0
38	36.10840297400496	37.0	34.0	40.0	31.0	41.0
39	35.97226590577282	37.0	34.0	40.0	30.0	41.0
40	35.84258553701682	37.0	34.0	40.0	30.0	41.0
41	35.67119203226603	36.0	34.0	40.0	30.0	41.0
42	35.54046316206126	36.0	34.0	39.0	30.0	41.0
43	35.405001291269656	36.0	33.0	39.0	30.0	41.0
44	35.30096193870199	36.0	33.0	39.0	29.0	41.0
45	35.23886395294846	35.0	33.0	39.0	30.0	40.0
46	35.17887409984045	35.0	33.0	39.0	29.0	40.0
47	35.12606165808399	35.0	33.0	39.0	29.0	40.0
48	35.14488262723686	35.0	33.0	39.0	30.0	40.0
49	35.12601459890728	35.0	33.0	39.0	29.0	40.0
50	35.52967950132972	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	18.0
22	42.0
23	97.0
24	225.0
25	571.0
26	1345.0
27	3172.0
28	7021.0
29	15140.0
30	29894.0
31	52627.0
32	83600.0
33	121737.0
34	167487.0
35	195967.0
36	243737.0
37	280127.0
38	274908.0
39	247583.0
40	119666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.604221432806895	16.358729645966374	31.037048921226734
2	28.078024202072015	25.476783854011405	28.110003111168446	18.335188832748138
3	26.210672717003998	19.046042040883137	27.424299417983853	27.31898582412901
4	21.603758551648106	21.942734988070242	30.039306957418187	26.414199502863468
5	22.360520464875773	22.281657222951534	26.343303887442914	29.01451842472978
6	25.354017364005628	23.880169065446193	26.347260599924333	24.41855297062385
7	30.931355916585996	20.229207475910123	23.164979734043882	25.67445687346
8	26.80179472142034	19.584480147601635	26.19067234843352	27.423052782544506
9	30.000986468043312	21.72256832917246	23.339129284767306	24.937315918016917
10	26.652577879483957	19.778467462273017	26.22850502394082	27.340449634302207
11	28.209842349398308	22.738359406081194	25.19282198154329	23.858976262977205
12	28.701558727911518	19.42501921444623	26.51864587206485	25.354776185577403
13	26.485095118284026	22.016286478992026	23.164004106308735	28.334614296415218
14	28.061330127492862	21.916284636139636	24.268848314819895	25.753536921547603
15	24.870539619700306	22.61835719465833	26.405635659410525	26.105467526230836
16	28.538466291519736	24.2297148023324	20.958218200226998	26.27360070592087
17	28.70470241728032	21.183534005504708	23.10476182216908	27.007001755045895
18	29.11164758591757	21.738178372934787	21.82018530422783	27.329988736919812
19	29.13235257451899	22.003549116894295	22.363935161948785	26.500163146637934
20	29.77876015059356	20.774637581397165	23.49636795474822	25.950234313261056
21	30.282726077336925	25.073090777824632	20.387259169003656	24.256923975834784
22	29.64466553855193	23.668186839215466	21.928425781288112	24.758721840944496
23	28.616408107249676	25.523342977594165	21.37627468473674	24.483974230419424
24	30.225272444045036	24.807178018456707	21.147489980845176	23.820059556653074
25	28.41661269607789	26.410388698009857	21.498260134578864	23.674738471333384
26	28.91847623912452	26.008761458466356	21.206338454363237	23.866423848045883
27	29.219357109693995	25.471343790859947	21.752916652865395	23.556382446580663
28	29.103053868809543	25.122775161806416	21.70069449494582	24.073476474438223
29	29.201309357190684	24.45652767439655	22.290685343303913	24.05147762510885
30	28.065080331647774	25.861931848855647	22.493383820822434	23.579603998674152
31	26.224974243800325	24.343246243349736	25.132653228853513	24.299126283996422
32	27.10997851503148	23.66491979122795	25.040147332268266	24.184954361472304
33	25.26509017469381	23.520788138147044	26.00738460137471	25.206737085784432
34	25.846800753155335	22.33433395168846	28.049350666631987	23.769514628524217
35	26.66105369740593	23.522376670592077	25.274197828429017	24.54237180357298
36	26.14815817934586	24.379582445211625	23.126782040555856	26.34547733488666
37	24.987231318496868	25.778545739386367	26.376251315158566	22.857971626958204
38	26.703385431321138	25.0609678604375	23.144645419016914	25.091001289224447
39	26.089544558181686	25.65469321346439	24.00399904504238	24.25176318331154
40	26.317958716876593	25.017881737780378	23.041089702066234	25.62306984327679
41	24.852745081899744	25.967898592476747	23.6411227261503	25.53823359947321
42	25.139472811493736	27.002497940669894	23.87244990746387	23.9855793403725
43	24.18031561348901	26.9663998042707	23.63968139620231	25.213603186037975
44	23.077954926063754	26.228041425870842	24.524527639142995	26.16947600892241
45	24.618831872353	24.107652910469813	25.594687003137707	25.678828214039484
46	24.165672478475226	24.53357002199192	23.80323698092596	27.497520518606887
47	25.34286241155072	24.885204528725158	24.434861856813892	25.337071202910234
48	24.47146930627063	24.298553266111355	24.737863268611346	26.492114159006668
49	26.66507390618222	23.971254864730625	22.783348946577554	26.580322282509606
50	23.160732868720633	27.319647230719095	23.956723744652223	25.562896155908042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29156.0
1	28822.0
2	17330.0
3	6161.5
4	6052.5
5	4589.5
6	2108.5
7	1030.5
8	1049.0
9	987.0
10	904.5
11	855.5
12	851.0
13	922.5
14	1128.0
15	1452.0
16	1900.5
17	2812.0
18	4501.5
19	7224.0
20	11057.0
21	14151.5
22	14929.0
23	15000.5
24	14907.0
25	14634.0
26	14473.5
27	14762.5
28	15511.5
29	16271.5
30	17234.0
31	19127.0
32	21928.0
33	23880.0
34	25936.5
35	29601.0
36	33375.5
37	36775.0
38	40804.0
39	47791.0
40	56457.0
41	66856.5
42	79157.5
43	88338.5
44	94167.0
45	99361.0
46	107281.5
47	115879.5
48	128467.5
49	142026.0
50	154808.5
51	168679.5
52	177890.0
53	177487.5
54	167418.5
55	151201.5
56	134311.0
57	124197.0
58	116393.0
59	109359.0
60	108250.0
61	108321.0
62	103731.0
63	96589.0
64	87184.5
65	78421.0
66	72190.0
67	67759.0
68	61504.5
69	53432.0
70	46234.0
71	40034.5
72	35702.0
73	32538.0
74	28594.0
75	24417.0
76	20949.0
77	16669.0
78	13229.0
79	10824.5
80	8332.0
81	6219.5
82	4600.0
83	3326.0
84	2467.0
85	1960.0
86	1503.0
87	1103.5
88	821.0
89	615.0
90	401.5
91	216.5
92	155.0
93	142.5
94	106.5
95	59.5
96	34.0
97	30.0
98	15.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	22986.0
25	20910.0
26	19833.0
27	21109.0
28	20343.0
29	17114.0
30	18227.0
31	16287.0
32	19008.0
33	15838.0
34	17813.0
35	18321.0
36	17560.0
37	18046.0
38	19198.0
39	21695.0
40	22053.0
41	22993.0
42	24212.0
43	25174.0
44	24826.0
45	30000.0
46	79086.0
47	208330.0
48	308739.0
49	414733.0
50	380532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.532768978641673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.410833414785856	16.293994557803618
2	12.316137440714453	6.535624588473514
3	6.370552627246595	5.070852033750381
4	4.264978362875664	4.526467424043415
5	3.018910272104178	4.00500244084942
6	2.2119717042358933	3.5213840529469955
7	1.7353916477421985	3.2231321973905644
8	1.406794335823252	2.9860919290287993
9	1.1213186424627133	2.6776519642716554
>10	5.648805407712736	24.988328206004557
>50	0.27079567704501883	4.965556871834227
>100	0.18713035487540028	10.134322840557859
>500	0.024329200151514054	4.554060493370106
>1k	0.011823536522231127	6.148980112155136
>5k	2.2737570235059864E-4	0.3685502875196786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	6109	0.33111721300013114	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	4637	0.2513325448815859	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4004	0.21702296952897776	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	3754	0.20347258431862703	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	3644	0.19751041482607268	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	3579	0.1939873146713815	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3375	0.18293020033973525	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3206	0.17377013993753815	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	3039	0.16471848261702385	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2986	0.16184580095242948	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	2951	0.15994874702298037	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2904	0.15740127460343442	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2547	0.13805132452305355	Illumina Single End Adapter 1 (100% over 32bp)
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	2531	0.1371840998695911	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCA	2421	0.13122193037703678	No Hit
AAAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTT	2223	0.12049002529043895	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	2113	0.11452785579788463	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	2090	0.11328122035853234	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2058	0.11154677105160746	Illumina Single End Adapter 1 (100% over 32bp)
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT	1993	0.10802367089691625	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	1985	0.10759005857018504	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	1966	0.10656022929419837	No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	1881	0.10195309832267913	Illumina Single End Adapter 2 (100% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.4754559162607875	0.0	0.0	0.0	0.0
2	1.0491250245261972	0.0	0.0	0.0	0.0
3	1.4305954689679918	0.0	0.0	0.0	0.0
4	1.714503139895261	0.0	0.0	0.0	0.0
5	2.0452951436503435	0.0	0.0	0.0	0.0
6	2.29586886696015	0.0	0.0	0.0	0.0
7	2.4815633458827966	0.0	0.0	0.0	0.0
8	2.647636867020856	0.0	0.0	0.0	0.0
9	2.780647448245659	0.0	0.0	0.0	0.0
10	2.8878038944891125	0.0	0.0	0.0	0.0
11	3.0081855166978686	0.0	0.0	0.0	0.0
12	3.101032756159192	0.0	0.0	0.0	0.0
13	3.192091344772749	0.0	0.0	0.0	0.0
14	3.275724322291034	0.0	0.0	0.0	0.0
15	3.3893307518946147	0.0	0.0	0.0	0.0
16	3.4629906458980817	0.0	0.0	0.0	0.0
17	3.5347534859720993	0.0	0.0	0.0	0.0
18	3.6046734736575092	0.0	0.0	0.0	0.0
19	3.8150838552038357	0.0	0.0	0.0	0.0
20	3.881426541193713	0.0	0.0	0.0	0.0
21	3.984734678037427	0.0	0.0	0.0	0.0
22	4.029992964639999	0.0	0.0	0.0	0.0
23	4.080400397622504	0.0	0.0	0.0	0.0
24	4.122894405642164	0.0	0.0	0.0	0.0
25	4.175795109503373	0.0	0.0	0.0	0.0
26	4.32674640074668	0.0	0.0	0.0	0.0
27	4.368589990276243	0.0	0.0	0.0	0.0
28	4.412710044521146	0.0	0.0	0.0	0.0
29	4.458022532664558	0.0	0.0	0.0	0.0
30	4.498836292918135	0.0	0.0	0.0	0.0
31	4.5379156038647865	0.0	0.0	0.0	0.0
32	4.572170977676554	0.0	0.0	0.0	0.0
33	4.6044208944771885	0.0	0.0	0.0	0.0
34	4.633310315745656	0.0	0.0	0.0	0.0
35	4.661169907738137	0.0	0.0	0.0	0.0
36	4.684422368759099	0.0	0.0	0.0	0.0
37	4.703121900349383	0.0	0.0	0.0	0.0
38	4.714558425466919	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	45	2.8079023E-5	86.29065	44
CGTGCGC	600	0.0	82.515434	44
ACTTCTA	365	0.0	74.47001	44
TCGTGCG	750	0.0	73.46553	43
ACGACAC	220	0.0	70.60144	44
ACATTGG	235	0.0	66.09496	44
ATGTTCT	505	0.0	65.35876	44
CGCTACC	280	0.0	62.406635	44
CGGGTAG	50	0.0046068556	58.24619	44
GGTCGAG	100	3.6464735E-7	58.24619	44
AACGACA	360	0.0	58.18551	43
GTTCAGG	1610	0.0	57.415573	43
TTCAGGA	985	0.0	57.16208	44
AGGTCGA	85	3.6379788E-12	53.572334	43
CGGGATA	55	0.006717226	52.95108	44
ACATTTG	235	0.0	49.571224	44
TACGCTA	420	0.0	49.349632	42
CTCGTGC	970	0.0	49.180046	42
AATCCCG	250	0.0	49.1794	43
AAACCGT	100	2.484487E-5	48.53849	44
>>END_MODULE