FastQCFastQC Report
Tue 24 May 2016
ERR841219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649565
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC190700.7197407876387256No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG144670.5460141570408727No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC110000.4151624889368632No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT108820.4107089276919041No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA95690.3611536233306222No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA75740.2858582446552547No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA71990.2717049779869526No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA69690.26302430776372726No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA65050.24551199913948138No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG63630.24015262882775096No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT56910.21478997495815352No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG51950.19606992091154588No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA50820.1918050698888308No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC49770.18784215522170622No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG48990.18489827575469936Illumina Single End Adapter 1 (100% over 32bp)
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA47620.17972761566521298No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC46120.1740663089978921No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC42180.15919594348506264No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG41390.156214321973607No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC40240.15187398686199433No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG39540.14923204375057794No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG38650.14587300179463422No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG38540.14545783930569733No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT35080.13239909192641056No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG34910.13175747717078087No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG34200.12907779201491568No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG32120.12122744676956407No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA31820.12009518543609989No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC31200.11775517868027392No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT30540.11526420374665275No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG30470.11500000943551111No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT30350.11454710490212544No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC29740.11224484019074829No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG29060.1096783811682295No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC28780.10862160392366295No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC28260.10665901761232503No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC28080.10597966081224654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA15800.0167.3418944
AATCGCA1400.0116.4758544
CGTGCGC7250.0102.8200744
ACATTGG5150.0101.77502444
GAACACA13000.096.7645644
ATTAGTC2150.086.679744
ACTTCTA9450.085.0458744
TTAAGCC15100.082.8637543
TCGTGCG23000.082.5336143
CTATCAT11900.081.2394644
CCCATAC4800.080.0771544
CATGACC6150.079.5444944
GACCGTA500.002238507169.8855144
AACGACA3450.067.5713743
CACATGC19850.065.71937644
ACCACTC2750.059.29680344
AATCCCG5400.059.02918243
TAATTCC4150.058.9395944
TGAACAC10200.058.3036343
ATTAAGC19850.058.13479242
Produced by FastQC (version 0.11.5)