##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841219.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2649565
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.42477916186242	37.0	35.0	39.0	30.0	39.0
2	36.42253313279727	37.0	35.0	39.0	32.0	39.0
3	36.72622524829548	38.0	35.0	39.0	32.0	39.0
4	37.16686739143973	38.0	35.0	40.0	33.0	41.0
5	37.61470731988081	39.0	36.0	40.0	33.0	41.0
6	37.62598917180745	39.0	36.0	40.0	33.0	41.0
7	37.580119000666144	39.0	36.0	40.0	33.0	41.0
8	37.447168874890785	39.0	36.0	40.0	33.0	41.0
9	37.367195369805984	39.0	36.0	40.0	32.0	41.0
10	37.171538724281156	38.0	35.0	40.0	32.0	41.0
11	37.04687826114853	38.0	35.0	40.0	31.0	41.0
12	36.97455016200772	38.0	35.0	40.0	31.0	41.0
13	36.8284710886504	38.0	35.0	40.0	31.0	41.0
14	36.79050674355979	38.0	35.0	40.0	31.0	41.0
15	36.76403485100384	38.0	35.0	40.0	31.0	41.0
16	36.73753389707367	38.0	35.0	40.0	31.0	41.0
17	36.7098002879718	38.0	35.0	40.0	31.0	41.0
18	36.591550688509244	38.0	35.0	40.0	31.0	41.0
19	36.51781179174695	38.0	35.0	40.0	31.0	41.0
20	36.40703662676704	38.0	35.0	40.0	30.0	41.0
21	36.208068494262264	38.0	34.0	40.0	30.0	41.0
22	36.26858220122926	38.0	34.0	40.0	30.0	41.0
23	36.31723131910332	38.0	34.0	40.0	30.0	41.0
24	36.29208417230753	38.0	34.0	40.0	30.0	41.0
25	36.291749200845416	38.0	34.0	40.0	30.0	41.0
26	36.26904461798279	38.0	34.0	40.0	30.0	41.0
27	36.27498210432875	38.0	34.0	40.0	30.0	41.0
28	36.2739083299688	38.0	34.0	40.0	31.0	41.0
29	36.293938928627114	38.0	34.0	40.0	31.0	41.0
30	36.28181078119834	38.0	34.0	40.0	31.0	41.0
31	36.211877832474215	37.0	34.0	40.0	31.0	41.0
32	36.135193330227104	37.0	34.0	40.0	31.0	41.0
33	36.06954790573345	37.0	34.0	40.0	31.0	41.0
34	35.99984446674907	37.0	34.0	40.0	30.0	41.0
35	35.89346743600664	37.0	34.0	40.0	30.0	41.0
36	35.77589915946482	36.0	34.0	40.0	30.0	41.0
37	35.71698958014987	36.0	34.0	39.0	30.0	41.0
38	35.619881062679106	36.0	34.0	39.0	30.0	41.0
39	35.45140139211541	36.0	34.0	39.0	30.0	41.0
40	35.33779179760678	35.0	33.0	39.0	30.0	40.0
41	35.17302969781252	35.0	33.0	39.0	29.0	40.0
42	35.02083618304421	35.0	33.0	39.0	29.0	40.0
43	34.92005422037848	35.0	33.0	39.0	29.0	40.0
44	34.813430168436895	35.0	33.0	38.0	29.0	40.0
45	34.739489438672095	35.0	33.0	38.0	29.0	40.0
46	34.63984788477377	35.0	33.0	38.0	29.0	40.0
47	34.620978858800584	35.0	33.0	38.0	29.0	40.0
48	34.65077708224602	35.0	33.0	38.0	29.0	40.0
49	34.7558931239296	35.0	33.0	38.0	29.0	40.0
50	35.445043432858135	36.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	28.0
22	79.0
23	184.0
24	550.0
25	1301.0
26	3150.0
27	6995.0
28	15586.0
29	31622.0
30	58860.0
31	98889.0
32	151381.0
33	218038.0
34	290589.0
35	294211.0
36	343822.0
37	377812.0
38	355577.0
39	290842.0
40	110040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.852107798827355	16.454550086523636	33.69334211464901
2	25.365069360442188	21.400795979717422	31.461579542302225	21.77255511753816
3	27.625364918392265	17.607682770567997	24.651857946493102	30.115094364546636
4	20.270384006431243	20.254192669362705	31.92388184475565	27.551541479450396
5	21.9436398050246	20.54808996948556	28.766118211857417	28.742152013632428
6	26.38723715024919	19.595933672131086	26.428036300298352	27.58879287732137
7	29.891208556876318	20.323147384570674	21.80074842474142	27.98489563381159
8	28.04011224484019	17.718380186936347	27.675863773864766	26.565643794358696
9	27.289422980753447	20.428711882893985	24.756478893705193	27.525386242647375
10	29.230232132444385	19.118421325764796	26.452832823501215	25.198513718289604
11	29.549454344392384	18.71405306154029	25.064114298007407	26.672378296059918
12	29.23026987448883	18.3993221528817	24.070819172203738	28.299588800425727
13	26.413618839318907	19.202850279196774	25.746113041197326	28.63741784028699
14	26.105341820261064	20.551335785308154	21.937299141557197	31.40602325287358
15	24.675635434495852	22.535623772204115	24.186083375950393	28.60265741734964
16	26.736690739800682	23.84047947493268	24.33591929241215	25.086910492854486
17	27.53100980727025	22.745507281383926	18.896309394183575	30.827173517162247
18	25.984189857580397	21.421327651897577	22.23848065625867	30.356001834263356
19	27.19159560154214	19.686061674274836	24.664992177961288	28.45735054622174
20	27.498966811533215	18.926691739964863	24.860684678428346	28.713656770073577
21	28.761211746079073	22.964562107364795	22.74346921098369	25.53075693557244
22	28.16084904503192	21.000013209715558	22.903872899891116	27.935264845361406
23	28.263129985488185	22.061998856416054	24.133999354611042	25.54087180348472
24	30.23975633736104	21.205405415605956	21.04987045043243	27.504967796600578
25	27.094448592870112	23.548383652168873	23.43957964201841	25.917588112942607
26	28.557142328838047	22.887595226942135	23.06945865486551	25.485803789354307
27	27.808959383727906	22.55756651532301	22.818895806949936	26.814578293999148
28	27.299333252505942	21.659229369691534	24.05419201841295	26.987245359389572
29	30.14543215290085	20.83853421739788	24.614672864722138	24.40136076497914
30	27.041901178264975	23.373927721434722	24.603645318350118	24.980525781950185
31	27.439235862854574	22.304519753757265	25.836112351974204	24.420132031413956
32	25.71750229917864	21.350729841834397	26.636685865113225	26.295081993873737
33	25.374834113736846	19.585285369581648	28.286418019820452	26.753462496861058
34	24.32340962066477	21.74105994873624	29.04714109147014	24.888389339128846
35	26.768190061980192	20.640103248673274	24.89107089091763	27.70063579842891
36	25.81452224169785	21.07898289828983	24.293632370755873	28.812862489256446
37	23.992716519636883	23.29428948505145	25.869373699226067	26.843620296085597
38	27.719764710952145	22.84001637542824	22.20421882743315	27.23600008618646
39	26.172457856323444	23.128392203387175	25.15959851468202	25.539551425607364
40	24.917954474042677	22.50980205511538	23.412952858912544	29.1592906119294
41	25.469221512873098	24.83941523149094	23.27272694835865	26.418636307277314
42	24.965995331214277	23.709323621970107	25.49792631336121	25.82675473345441
43	23.62708347248311	23.962278134255367	25.462167881882653	26.94847051137887
44	22.709797414918114	23.559468890424924	23.678797479357407	30.051936215299555
45	25.065855529608193	21.634035040218407	27.70407223143542	25.59603719873798
46	23.26215465816841	23.2753210632689	25.823549028521125	27.638975250041565
47	28.131257744588602	23.005724560700155	23.52935364826997	25.333664046441275
48	23.485228718134625	23.995449701386654	24.185337162751306	28.333984417727414
49	25.883467550923733	22.902652771198483	21.235743176766388	29.978136501111397
50	22.20397489306346	28.344151569852933	23.62444021483255	25.827433322251053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3471.0
1	3350.5
2	3020.5
3	2744.5
4	2528.0
5	1959.0
6	1294.5
7	1076.5
8	1114.0
9	1127.5
10	1102.0
11	1058.5
12	1064.0
13	1137.0
14	1250.5
15	1354.0
16	1413.5
17	1584.0
18	1867.5
19	2322.0
20	2871.5
21	3621.0
22	4462.5
23	5589.0
24	6931.0
25	8325.0
26	10207.5
27	12298.0
28	14269.5
29	16553.5
30	19836.0
31	25874.0
32	33024.0
33	37283.5
34	43644.0
35	54381.5
36	61104.0
37	64377.5
38	68819.0
39	74949.0
40	82982.5
41	97662.5
42	120248.0
43	143396.0
44	153893.5
45	146213.0
46	142089.5
47	144036.5
48	154813.5
49	176429.0
50	203117.0
51	223041.5
52	226682.5
53	227602.5
54	222526.5
55	212485.0
56	203470.5
57	201607.5
58	200134.5
59	189557.5
60	184079.0
61	183555.5
62	180420.0
63	171462.5
64	153492.0
65	137811.5
66	122018.5
67	107273.0
68	95679.5
69	82684.0
70	70658.5
71	61484.5
72	56941.0
73	52442.0
74	44456.0
75	36895.5
76	32012.5
77	25384.0
78	19324.0
79	15488.0
80	12109.0
81	9109.5
82	6982.0
83	5555.5
84	4295.5
85	3163.0
86	2229.0
87	1467.0
88	948.5
89	701.5
90	548.5
91	394.5
92	276.5
93	218.5
94	126.5
95	40.0
96	23.5
97	21.0
98	11.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	27419.0
25	26242.0
26	24053.0
27	23210.0
28	21827.0
29	19653.0
30	24570.0
31	23017.0
32	26431.0
33	22083.0
34	23039.0
35	23813.0
36	21372.0
37	22286.0
38	24476.0
39	26907.0
40	27049.0
41	26981.0
42	29243.0
43	28825.0
44	29685.0
45	46330.0
46	157937.0
47	323222.0
48	502175.0
49	649557.0
50	448163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.421037820419297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.106038132549244	13.027976785646125
2	12.758307604082878	5.721089946313706
3	6.813628685342557	4.583058793450778
4	4.671366382657738	4.189475293544177
5	3.364120512517311	3.7713536621799486
6	2.559161504279075	3.442743412560137
7	1.9698962119813608	3.091698222979436
8	1.5106733571022595	2.7096691579111636
9	1.2249926217781009	2.4719045312359254
>10	6.451028452915822	24.497374553727866
>50	0.31056134943716757	4.824674984573817
>100	0.20925315913067774	9.183451008733678
>500	0.028071550675240236	4.356602447785524
>1k	0.02049961924310307	9.34363338128607
>5k	0.0016621312899813298	2.4905341300690593
>10k+	7.387250177694799E-4	2.2947596880026797
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	19070	0.7197407876387256	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	14467	0.5460141570408727	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	11000	0.4151624889368632	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	10882	0.4107089276919041	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	9569	0.3611536233306222	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7574	0.2858582446552547	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	7199	0.2717049779869526	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	6969	0.26302430776372726	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	6505	0.24551199913948138	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	6363	0.24015262882775096	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	5691	0.21478997495815352	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	5195	0.19606992091154588	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	5082	0.1918050698888308	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	4977	0.18784215522170622	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	4899	0.18489827575469936	Illumina Single End Adapter 1 (100% over 32bp)
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	4762	0.17972761566521298	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	4612	0.1740663089978921	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC	4218	0.15919594348506264	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	4139	0.156214321973607	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	4024	0.15187398686199433	No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG	3954	0.14923204375057794	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3865	0.14587300179463422	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	3854	0.14545783930569733	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	3508	0.13239909192641056	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG	3491	0.13175747717078087	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	3420	0.12907779201491568	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	3212	0.12122744676956407	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	3182	0.12009518543609989	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	3120	0.11775517868027392	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	3054	0.11526420374665275	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	3047	0.11500000943551111	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGT	3035	0.11454710490212544	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	2974	0.11224484019074829	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	2906	0.1096783811682295	No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAGCC	2878	0.10862160392366295	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2826	0.10665901761232503	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC	2808	0.10597966081224654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.15089269370632538	0.0	0.0	0.0	0.0
2	0.36538073230888846	0.0	0.0	0.0	0.0
3	0.47441749872148825	0.0	0.0	0.0	0.0
4	0.5540909545529171	0.0	0.0	0.0	0.0
5	0.688377148701768	0.0	0.0	0.0	0.0
6	0.78280774391268	0.0	0.0	0.0	0.0
7	0.8747851062344196	0.0	0.0	0.0	0.0
8	0.941550782864357	0.0	0.0	0.0	0.0
9	1.0002019199378012	0.0	0.0	0.0	0.0
10	1.0456055994097144	0.0	0.0	0.0	0.0
11	1.0918773459039504	0.0	0.0	0.0	0.0
12	1.1278077722192132	0.0	0.0	0.0	0.0
13	1.1560010794224713	0.0	0.0	0.0	0.0
14	1.183816966181241	0.0	0.0	0.0	0.0
15	1.2217477208522909	0.0	0.0	0.0	0.0
16	1.245411982721692	0.0	0.0	0.0	0.0
17	1.2725862547248321	0.0	0.0	0.0	0.0
18	1.303346020950609	0.0	0.0	0.0	0.0
19	1.3707910543806248	0.0	0.0	0.0	0.0
20	1.3874730380269968	0.0	0.0	0.0	0.0
21	1.4270644426537942	0.0	0.0	0.0	0.0
22	1.439896737766388	0.0	0.0	0.0	0.0
23	1.4547293612347687	0.0	0.0	0.0	0.0
24	1.4671842359028746	0.0	0.0	0.0	0.0
25	1.4823565377712946	0.0	0.0	0.0	0.0
26	1.5357615306663546	0.0	0.0	0.0	0.0
27	1.548140921245563	0.0	0.0	0.0	0.0
28	1.5600296652469368	0.0	0.0	0.0	0.0
29	1.573012928537326	0.0	0.0	0.0	0.0
30	1.5856565134276759	0.0	0.0	0.0	0.0
31	1.5971300949401128	0.0	0.0	0.0	0.0
32	1.6090565809859354	0.0	0.0	0.0	0.0
33	1.6175862830313654	0.0	0.0	0.0	0.0
34	1.6290598645438026	0.0	0.0	0.0	0.0
35	1.640231509700649	0.0	0.0	0.0	0.0
36	1.650535087835173	0.0	0.0	0.0	0.0
37	1.6594799523695398	0.0	0.0	0.0	0.0
38	1.6653677113035537	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	1580	0.0	167.34189	44
AATCGCA	140	0.0	116.47585	44
CGTGCGC	725	0.0	102.82007	44
ACATTGG	515	0.0	101.775024	44
GAACACA	1300	0.0	96.76456	44
ATTAGTC	215	0.0	86.6797	44
ACTTCTA	945	0.0	85.04587	44
TTAAGCC	1510	0.0	82.86375	43
TCGTGCG	2300	0.0	82.53361	43
CTATCAT	1190	0.0	81.23946	44
CCCATAC	480	0.0	80.07715	44
CATGACC	615	0.0	79.54449	44
GACCGTA	50	0.0022385071	69.88551	44
AACGACA	345	0.0	67.57137	43
CACATGC	1985	0.0	65.719376	44
ACCACTC	275	0.0	59.296803	44
AATCCCG	540	0.0	59.029182	43
TAATTCC	415	0.0	58.93959	44
TGAACAC	1020	0.0	58.30363	43
ATTAAGC	1985	0.0	58.134792	42
>>END_MODULE