FastQCFastQC Report
Tue 24 May 2016
ERR841218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5331877
Sequences flagged as poor quality0
Sequence length24-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC386370.7246416224530311No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG340990.6395308819014393No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG210380.3945702423367981No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC180110.337798490100203No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT173930.32620782512424795No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC166580.3124228109538161No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT152470.2859593347708509No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC148090.27774459163255266No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC124080.2327135453424751No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG110010.2063250896447911No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC106610.19994834839588385No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG98210.1841940464868188No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG97790.18340633139136556No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG95460.17903638812373204Illumina Single End Adapter 1 (100% over 32bp)
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT94730.17766726426734902No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT90560.16984637867677743No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG90410.1695650518569727No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG89040.16699560023608948No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG86910.16300075939486228No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA85830.1609752062922682No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA79960.14996595007724295No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG79190.14852180573557866No Hit
AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT74190.1391442450754209No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA71560.13421164816817793No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC67440.12648453818420793No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC64230.12046414424038664No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG64210.120426633997746No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG63000.11815726431798784No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA58810.11029886848477563No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG58760.11020509287817405No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTTC57640.10810451929029871No Hit
CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG56630.10621025203694683No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG55040.10322818774701667Illumina Single End Adapter 1 (100% over 31bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG54390.10200910486119616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG5000.0128.7764944
CGTGCGC28550.0121.7851244
ATGTTCT15600.0110.7532944
TATACGC2000.096.5823744
CTATGCG2750.089.7533144
TCCTTGT28650.081.6558444
TCGTGCG48000.077.0077443
AATCCCG48150.075.3157343
CGCTACC7000.068.98740444
TAAGCCA13700.066.5815244
ATCCTTG30400.064.3988543
CACATGC14450.062.3830844
ACGCTAC9050.060.26146743
ACGACAC3500.058.2560344
AATCCTG23150.055.5725743
TAATCCC99200.053.78973842
TTTGGAC20700.053.14275443
AGGTCCC18450.051.79166843
TAATCCT36950.051.53103342
TTCAGGA22100.050.98616444
Produced by FastQC (version 0.11.5)