##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841218.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5331877 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.28617651907574 35.0 33.0 37.0 24.0 39.0 2 34.077609817330746 35.0 33.0 37.0 27.0 39.0 3 34.62958747923105 35.0 33.0 37.0 30.0 39.0 4 34.96582423037891 35.0 34.0 38.0 31.0 39.0 5 35.46580838230139 35.0 34.0 39.0 32.0 41.0 6 35.53513575050587 35.0 34.0 39.0 32.0 41.0 7 35.49031063544789 35.0 34.0 39.0 32.0 41.0 8 35.36761538197524 35.0 34.0 39.0 31.0 41.0 9 35.32442027451121 35.0 34.0 39.0 31.0 41.0 10 35.234587744616015 35.0 34.0 39.0 31.0 41.0 11 35.095257448737094 35.0 34.0 38.0 31.0 41.0 12 35.02355793278802 35.0 34.0 38.0 31.0 40.0 13 34.91018509991885 35.0 34.0 38.0 30.0 40.0 14 34.8991570885825 35.0 33.0 38.0 30.0 40.0 15 34.883821400981304 35.0 33.0 38.0 30.0 40.0 16 34.86193755032234 35.0 33.0 38.0 30.0 40.0 17 34.85231486022652 35.0 33.0 38.0 30.0 40.0 18 34.80478788239114 35.0 33.0 38.0 30.0 40.0 19 34.735238453550224 35.0 33.0 38.0 30.0 40.0 20 34.66225327403464 35.0 33.0 38.0 30.0 40.0 21 34.46932196672954 35.0 33.0 38.0 29.0 40.0 22 34.4665287665113 35.0 33.0 38.0 30.0 40.0 23 34.53455490439858 35.0 33.0 38.0 30.0 40.0 24 34.51664057516706 35.0 33.0 38.0 30.0 40.0 25 34.514398159291765 35.0 33.0 38.0 30.0 40.0 26 34.50141293877482 35.0 33.0 37.0 30.0 40.0 27 34.534646963248676 35.0 33.0 37.0 30.0 40.0 28 34.57535518406389 35.0 33.0 37.0 30.0 40.0 29 34.628524251854685 35.0 33.0 37.0 30.0 40.0 30 34.661963961889924 35.0 33.0 37.0 30.0 40.0 31 34.61040118686357 35.0 33.0 37.0 30.0 40.0 32 34.60593380937833 35.0 33.0 37.0 30.0 40.0 33 34.55213787361517 35.0 33.0 37.0 30.0 40.0 34 34.55866886685044 35.0 33.0 37.0 30.0 40.0 35 34.52669028198284 35.0 33.0 36.0 30.0 40.0 36 34.39206352673982 35.0 33.0 36.0 30.0 40.0 37 34.38883231845552 35.0 33.0 36.0 30.0 40.0 38 34.36868036727956 35.0 33.0 36.0 30.0 40.0 39 34.22355213815203 35.0 33.0 36.0 30.0 40.0 40 34.15327882218854 35.0 33.0 35.0 29.0 40.0 41 34.084158409275396 35.0 33.0 35.0 29.0 39.0 42 34.00616101813519 35.0 33.0 35.0 29.0 39.0 43 33.96384423286324 35.0 33.0 35.0 29.0 39.0 44 33.93238515187601 35.0 33.0 35.0 29.0 39.0 45 33.9332433832666 35.0 33.0 35.0 30.0 39.0 46 33.860012733548835 35.0 33.0 35.0 29.0 39.0 47 33.83691164453056 35.0 33.0 35.0 29.0 39.0 48 33.92268381911464 35.0 33.0 35.0 29.0 39.0 49 33.86403457658156 35.0 33.0 35.0 29.0 39.0 50 34.476028043024314 35.0 33.0 37.0 29.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 26.0 21 127.0 22 519.0 23 1801.0 24 5066.0 25 11938.0 26 26123.0 27 50746.0 28 91433.0 29 154039.0 30 244905.0 31 366803.0 32 530281.0 33 792579.0 34 1291519.0 35 318638.0 36 312370.0 37 353895.0 38 349012.0 39 300526.0 40 129531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 47.86426993720973 17.32690007665218 34.80882998613809 2 27.90358817354564 20.61210339248261 28.885268733693593 22.599039700278155 3 30.6521699581592 16.21594046524329 25.325546707097708 27.8063428694998 4 20.480404930571357 17.812320126664588 29.474573400699228 32.23270154206483 5 25.160670435570815 18.98522790379448 27.23478054726319 28.61932111337152 6 27.482798271603038 18.398867790836135 25.014830612184042 29.103503325376785 7 30.90166933708336 20.239270335756057 21.70578953715549 27.153270790005095 8 28.229177079666318 16.229181580895432 28.510297593136524 27.031343746301722 9 28.701543565239785 17.167875402977227 23.31375986355274 30.816821168230252 10 28.550433552762 20.290734388659004 25.45972084502324 25.699111213555753 11 34.39919938138108 16.440214205991623 24.184766452789518 24.975819959837782 12 30.380464515591786 16.684480906067414 22.249856851536524 30.685197726804276 13 27.860526414994197 17.475534413115682 27.30147375867823 27.362465413211893 14 28.31370641145698 18.30000954635675 21.475139055158248 31.911144987028024 15 26.23674552132392 19.079041020638698 22.965045892844117 31.719167565193267 16 27.602624741718536 20.467745223680144 26.585722063731026 25.343907970870294 17 27.650787893269108 19.329084298081145 19.435425835967337 33.58470197268242 18 27.358901940161033 19.441464985032475 21.372848623477246 31.826784451329242 19 28.0120302850197 17.1816041517837 26.773760910088512 28.03260465310809 20 28.41125179744394 17.115942472041272 25.736208843527336 28.736596886987453 21 30.583788785825327 20.01004524297916 20.951683619108245 28.45448235208727 22 28.682788443919467 18.97142413450273 25.65503667845301 26.6907507431248 23 29.14530849079977 20.735980968803293 22.7075568322375 27.411153708159436 24 32.56157259441656 22.087831358450316 20.4094543066166 24.94114174051652 25 28.83757910477747 21.857159373264622 22.524946789658525 26.780314732299388 26 31.88230015176505 20.89367181075158 21.529829260486107 25.694198776997258 27 29.02347638687867 21.77575820943002 23.624755834792317 25.57600956889899 28 29.82937709329706 20.444853962821583 24.14902568752773 25.57674325635363 29 32.14464916150941 19.099028636008654 25.004227254030837 23.752094948451095 30 28.453975815963094 21.826377611033372 22.727518579444897 26.992127993558633 31 27.874542773138828 20.023539540804837 28.08155944554595 24.020358240510383 32 28.36562080942457 19.45555761294727 25.366811965188933 26.81200961243923 33 26.3012837648941 19.10573153784464 25.99194356413621 28.601041133125054 34 25.7145310988781 17.94038825609348 31.244449424058235 25.100631220970175 35 31.014183739686658 18.873157816188808 23.58774741727399 26.52491102685054 36 27.04831220074537 20.204080260842275 21.964622783199854 30.782984755212496 37 25.557822610375908 20.374360669353234 26.99216912652708 27.075647593743774 38 31.292245177729335 20.283158542657755 21.32773353225113 27.096862747361783 39 27.66958120720968 20.11506186871279 26.075634001700536 26.139722922376997 40 27.61861813679607 19.35702322458064 22.81686187707144 30.207496761551848 41 26.722269578550296 21.074695863316666 23.30140612924505 28.90162842888799 42 26.07652378893449 20.443880363839444 27.782641569501372 25.696954277724693 43 24.302205913633472 25.152855305425494 24.0675939511388 26.477344829802234 44 23.3840247137372 23.739123204723224 23.959539432470013 28.917312649069558 45 26.369299600048425 18.92622533001919 28.666342611424522 26.038132458507864 46 24.90302131096943 20.58056285689032 23.57743713460174 30.93897869753851 47 27.554285272324456 20.126486694422027 24.111265345250974 28.207962688002542 48 23.93383095476644 20.708488744546287 25.619742792723837 29.73793750796344 49 29.328174522375345 20.496554611464223 21.725627542702895 28.449643323457536 50 24.970343713615552 22.83080103400421 23.54484659044993 28.65400866193031 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2960.0 1 2943.0 2 2439.5 3 1949.5 4 1869.0 5 1504.0 6 1090.0 7 1069.0 8 1259.5 9 1400.0 10 1459.5 11 1495.0 12 1507.0 13 1528.0 14 1606.5 15 1670.5 16 1802.5 17 2139.5 18 2580.0 19 3133.0 20 3687.0 21 4474.0 22 5587.5 23 7290.5 24 9304.0 25 11221.5 26 13700.0 27 16448.5 28 19017.0 29 22155.0 30 26291.5 31 32194.0 32 39442.0 33 46067.5 34 54573.5 35 65687.5 36 75034.5 37 83430.5 38 93273.5 39 108068.5 40 125227.5 41 149122.0 42 182370.0 43 211898.0 44 227469.0 45 232528.5 46 244970.5 47 265575.5 48 293847.5 49 327818.0 50 367824.0 51 410154.0 52 445923.5 53 464672.5 54 461404.0 55 460058.5 56 471186.5 57 491893.0 58 507987.5 59 478394.0 60 430524.5 61 409813.5 62 397444.5 63 379926.5 64 355233.5 65 326557.5 66 287648.0 67 251720.0 68 225933.5 69 201364.5 70 178497.5 71 158229.0 72 141191.0 73 124189.5 74 105252.5 75 88416.0 76 76450.0 77 61975.0 78 48816.5 79 38778.0 80 28879.0 81 21253.0 82 15876.0 83 11547.5 84 8411.0 85 6343.0 86 4612.5 87 3137.0 88 2111.5 89 1509.5 90 1034.0 91 640.5 92 463.5 93 398.5 94 277.0 95 136.5 96 87.0 97 78.0 98 49.5 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 55647.0 25 52366.0 26 49601.0 27 46288.0 28 41931.0 29 38665.0 30 50680.0 31 44366.0 32 51903.0 33 45078.0 34 44350.0 35 50424.0 36 40798.0 37 42471.0 38 49364.0 39 53223.0 40 51098.0 41 50662.0 42 53750.0 43 53381.0 44 53809.0 45 80680.0 46 300810.0 47 657763.0 48 998860.0 49 1271592.0 50 1002317.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.219568715576635 #Duplication Level Percentage of deduplicated Percentage of total 1 55.35402638648589 11.192345402053938 2 13.338171216868503 5.393841389191983 3 7.369724497046352 4.470379526486916 4 5.072275793742962 4.102369158237675 5 3.5532531531014593 3.592262314648715 6 2.6330382362475326 3.1943338529128615 7 2.0776741885940453 2.940677321740006 8 1.608613718850895 2.602038050009996 9 1.3097269079020872 2.3833901891669367 >10 6.994986119279309 24.256515032194937 >50 0.37775170131010605 5.286381805909667 >100 0.2567611266190932 10.350863073711725 >500 0.029627542188182605 4.103350444129818 >1k 0.020729167682805548 8.08183958177761 >5k 0.0025279472783909206 3.7566134794820765 >10k+ 0.001112296802492005 4.2927993783452205 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC 38637 0.7246416224530311 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG 34099 0.6395308819014393 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 21038 0.3945702423367981 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC 18011 0.337798490100203 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT 17393 0.32620782512424795 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC 16658 0.3124228109538161 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT 15247 0.2859593347708509 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC 14809 0.27774459163255266 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC 12408 0.2327135453424751 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG 11001 0.2063250896447911 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC 10661 0.19994834839588385 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG 9821 0.1841940464868188 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGG 9779 0.18340633139136556 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 9546 0.17903638812373204 Illumina Single End Adapter 1 (100% over 32bp) ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTGT 9473 0.17766726426734902 No Hit CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT 9056 0.16984637867677743 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG 9041 0.1695650518569727 No Hit ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG 8904 0.16699560023608948 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG 8691 0.16300075939486228 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA 8583 0.1609752062922682 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 7996 0.14996595007724295 No Hit CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG 7919 0.14852180573557866 No Hit AAACAGCCGGGGCTCCAGCGGGAGAACGATAATGCAAAGTGCTATGTTCT 7419 0.1391442450754209 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTA 7156 0.13421164816817793 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC 6744 0.12648453818420793 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGC 6423 0.12046414424038664 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 6421 0.120426633997746 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG 6300 0.11815726431798784 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA 5881 0.11029886848477563 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG 5876 0.11020509287817405 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTTC 5764 0.10810451929029871 No Hit CTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGCCG 5663 0.10621025203694683 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 5504 0.10322818774701667 Illumina Single End Adapter 1 (100% over 31bp) ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG 5439 0.10200910486119616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11701320191744859 0.0 0.0 0.0 0.0 2 0.24225990209451567 0.0 0.0 0.0 0.0 3 0.3101346861527376 0.0 0.0 0.0 0.0 4 0.3724579543001461 0.0 0.0 0.0 0.0 5 0.48436976321846886 0.0 0.0 0.0 0.0 6 0.5459990918770257 0.0 0.0 0.0 0.0 7 0.5999950861582141 0.0 0.0 0.0 0.0 8 0.6410312916070644 0.0 0.0 0.0 0.0 9 0.6767222874796249 0.0 0.0 0.0 0.0 10 0.708587238602841 0.0 0.0 0.0 0.0 11 0.7430966618322216 0.0 0.0 0.0 0.0 12 0.7674595644273114 0.0 0.0 0.0 1.312858492422087E-4 13 0.7876588300892913 0.0 0.0 0.0 1.312858492422087E-4 14 0.8129969989930376 0.0 0.0 0.0 1.312858492422087E-4 15 0.8421612126461282 0.0 0.0 0.0 1.312858492422087E-4 16 0.8604849661760765 0.0 0.0 0.0 1.312858492422087E-4 17 0.8810968445071032 0.0 0.0 0.0 1.312858492422087E-4 18 0.9042219090950523 0.0 0.0 0.0 1.312858492422087E-4 19 0.9556484517553574 0.0 0.0 0.0 1.312858492422087E-4 20 0.974759920380759 0.0 0.0 0.0 1.312858492422087E-4 21 1.0072437905075455 0.0 0.0 0.0 1.312858492422087E-4 22 1.0192470681525474 0.0 0.0 0.0 1.312858492422087E-4 23 1.0333321642641045 0.0 0.0 0.0 1.312858492422087E-4 24 1.045372952151747 0.0 0.0 0.0 1.312858492422087E-4 25 1.0647845027182734 0.0 0.0 0.0 1.312858492422087E-4 26 1.109402936339304 0.0 0.0 0.0 1.312858492422087E-4 27 1.1209560910726186 0.0 0.0 0.0 1.312858492422087E-4 28 1.1344035130592847 0.0 0.0 0.0 1.312858492422087E-4 29 1.1450939322118645 0.0 0.0 0.0 1.312858492422087E-4 30 1.1558406167284054 0.0 0.0 0.0 1.312858492422087E-4 31 1.1667936075794696 0.0 0.0 0.0 1.5004097056252422E-4 32 1.1769213730924402 0.0 0.0 0.0 1.5004097056252422E-4 33 1.18821195612727 0.0 0.0 0.0 1.5004097056252422E-4 34 1.1967455363280135 0.0 0.0 0.0 1.5004097056252422E-4 35 1.207285914510031 0.0 0.0 0.0 1.5004097056252422E-4 36 1.2179388234199702 0.0 0.0 1.8755121320315528E-5 1.5004097056252422E-4 37 1.22684750604712 0.0 0.0 1.8755121320315528E-5 1.5004097056252422E-4 38 1.2326991038990585 0.0 0.0 1.8755121320315528E-5 1.5004097056252422E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 500 0.0 128.77649 44 CGTGCGC 2855 0.0 121.78512 44 ATGTTCT 1560 0.0 110.75329 44 TATACGC 200 0.0 96.58237 44 CTATGCG 275 0.0 89.75331 44 TCCTTGT 2865 0.0 81.65584 44 TCGTGCG 4800 0.0 77.00774 43 AATCCCG 4815 0.0 75.31573 43 CGCTACC 700 0.0 68.987404 44 TAAGCCA 1370 0.0 66.58152 44 ATCCTTG 3040 0.0 64.39885 43 CACATGC 1445 0.0 62.38308 44 ACGCTAC 905 0.0 60.261467 43 ACGACAC 350 0.0 58.25603 44 AATCCTG 2315 0.0 55.57257 43 TAATCCC 9920 0.0 53.789738 42 TTTGGAC 2070 0.0 53.142754 43 AGGTCCC 1845 0.0 51.791668 43 TAATCCT 3695 0.0 51.531033 42 TTCAGGA 2210 0.0 50.986164 44 >>END_MODULE