FastQCFastQC Report
Tue 24 May 2016
ERR841217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16775634
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC820760.4892572167466219No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA765660.4564119603467744No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG730410.4353993416880697No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC643190.38340726794587915No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT560310.3340022797350014No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC470300.2803470795798239No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG403150.24031878616331284No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG384770.2293624193279372No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC368980.21994995837415146No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC363180.2164925629636412No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT354800.21149722269811086No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC316350.18857707553705572No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG315630.18814788162402685No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG301640.1798084054528133No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC298510.17794260413645172No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA293300.17483690929356233No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA284420.1695435176995397No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG278840.16621726487356603No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG276980.1651085139315748Illumina Single End Adapter 1 (100% over 32bp)
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG273980.16332020596062122No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG262100.15623850639564502No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA243350.1450615815771851No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG238770.14233143140819596Illumina Single End Adapter 1 (100% over 31bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA233710.13931515196385424No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC219320.13073723472984686No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAA207040.12341709410207687No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA201240.1199596986915666No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC198540.11835022151770835No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCG197330.11762893730275709No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG194370.11586447343808289No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC191380.11408212649369914No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG182500.10878873489967651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA104550.0149.6181344
ATTAGTC24500.0109.54584544
AATCGCA10350.092.8876944
CGTGCGC51400.091.1821844
TTAAGCC95000.079.3337143
TCGTGCG129000.073.5959143
CACATGC101500.073.3077744
CTATACG4850.072.2692544
ACATTGG23850.068.8624944
AATCCCG62100.061.85741843
ATTAAGC129650.059.05116342
ACTTCTA32350.058.50793544
TATTAGT21400.057.7732543
CTATCAT41700.054.9954444
AGGTCCC69900.054.2369443
CCTTAGC124700.054.21919643
CCCATAC24000.053.4104244
ACACATG68250.051.2032443
CTCGTGC155650.049.04109642
ATGTTCT35000.048.6416344
Produced by FastQC (version 0.11.5)