FastQCFastQC Report
Tue 24 May 2016
ERR841216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8703895
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG555690.6384383083665416Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA360610.4143087663626457No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC360470.4141479188340393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA351070.403348156199035No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG335510.3854711023053472Illumina Single End Adapter 1 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAA334710.38455197357045323No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG332060.3815073596361169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA307350.35311777083707924No Hit
AAAAAAAAAAAAAAAAAAAAAAAA291440.33483859812187533No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG271810.31228547678941443No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA266100.30572519544410864No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA228810.2628823072888632Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA226280.259975562664761No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC213540.24533843756157445No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA194750.2237504014007522Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT181870.2089524287689592No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA181580.20861924460256012Illumina Single End Adapter 1 (100% over 32bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC180230.20706821486242652No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA178160.2046899692608884Illumina Single End Adapter 1 (100% over 31bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC174510.2004964444079346No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC173380.19919817506989684No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC162720.18695078467743464No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA157750.18124069741190582No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG146260.1680397109569911No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG144600.1661325188320861No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA136190.15647017800651317Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG134810.15488468093882107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134400.1544136274621879No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT133480.15335662941705983No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA133040.15285110861286813Illumina Single End Adapter 1 (100% over 31bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA130710.15017414617248945No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC128490.14762356393315865No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG128080.1471525104565255No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG125070.14369428859148692No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA121570.13967310037632577Illumina Single End Adapter 1 (100% over 32bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC119580.137386767648277No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG118260.13587020523570192No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG114190.13119413779692884Illumina Single End Adapter 2 (100% over 34bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA111240.12780485058700733Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA96860.11128351157728811No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG96130.11044480660669735Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT93080.10694062830491408No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG92290.10603298867920626No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG88010.10111564994752349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA42900.0130.1001744
CTATACG6850.0117.5700644
CGTGCGC35050.0104.7338244
AATCGCA2850.082.14554644
TTAAGCC36850.079.04034443
TCGTGCG60000.077.7505543
ATGTTCT22050.076.44565644
AATCCCG49500.073.52711543
CCAGAAT20050.070.0592944
CTATCAT18000.069.1939344
TATACGC2800.066.88994644
CGCTACC11600.062.1615244
TTTGGAC37650.060.4821343
ACTTCTA15800.060.45496444
TTCAGGA32400.059.25127844
ATTAAGC51900.058.3896942
AATCCTG30250.057.134743
TGTGTTA38100.054.38847742
CTATGCG3150.053.5119644
TAATCCC103300.052.70674542
Produced by FastQC (version 0.11.5)