##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841216.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8703895
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.457419350761928	33.0	31.0	35.0	21.0	35.0
2	32.44116651223389	34.0	33.0	35.0	24.0	37.0
3	33.393200170728164	35.0	33.0	35.0	30.0	37.0
4	33.785501318662504	35.0	33.0	35.0	30.0	37.0
5	34.10784746369298	35.0	34.0	35.0	31.0	38.0
6	34.1397594984774	35.0	34.0	35.0	31.0	38.0
7	34.08718717309894	35.0	33.0	35.0	31.0	38.0
8	33.959083375890906	35.0	33.0	35.0	30.0	38.0
9	33.96464548343012	35.0	33.0	35.0	30.0	38.0
10	33.84752952557447	35.0	33.0	35.0	30.0	38.0
11	33.741545825173674	35.0	33.0	35.0	30.0	38.0
12	33.68626229980946	35.0	33.0	35.0	30.0	38.0
13	33.60387504674631	35.0	33.0	35.0	29.0	37.0
14	33.51186911147251	35.0	33.0	35.0	29.0	37.0
15	33.418313754933855	35.0	33.0	35.0	29.0	36.0
16	33.48087700966062	35.0	33.0	35.0	29.0	36.0
17	33.481592551380736	35.0	33.0	35.0	29.0	37.0
18	33.42722091661262	35.0	33.0	35.0	29.0	36.0
19	33.38894253664595	35.0	33.0	35.0	29.0	36.0
20	33.31824671598175	35.0	33.0	35.0	29.0	36.0
21	33.0080512230444	34.0	33.0	35.0	27.0	36.0
22	32.99787888066205	34.0	33.0	35.0	27.0	36.0
23	33.13321748481571	35.0	33.0	35.0	27.0	36.0
24	33.14588836377277	35.0	33.0	35.0	27.0	36.0
25	33.157405202621156	35.0	33.0	35.0	28.0	36.0
26	33.21011359714659	35.0	33.0	35.0	29.0	36.0
27	33.26537687194505	35.0	33.0	35.0	29.0	36.0
28	33.32387945607281	35.0	33.0	35.0	29.0	36.0
29	33.448356018398655	35.0	33.0	35.0	29.0	36.0
30	33.542645710363075	35.0	33.0	35.0	30.0	36.0
31	33.530339579002586	35.0	33.0	35.0	30.0	36.0
32	33.47078173855281	35.0	33.0	35.0	29.0	36.0
33	33.43351228927594	35.0	33.0	35.0	29.0	36.0
34	33.49356832517422	35.0	33.0	35.0	29.0	36.0
35	33.473811596991375	35.0	33.0	35.0	29.0	36.0
36	33.40417307760374	35.0	33.0	35.0	29.0	35.0
37	33.40166849380629	35.0	33.0	35.0	29.0	35.0
38	33.35019850268569	35.0	33.0	35.0	29.0	35.0
39	33.24866696876278	35.0	33.0	35.0	29.0	35.0
40	33.234725872008454	35.0	33.0	35.0	29.0	35.0
41	33.209146749935094	35.0	33.0	35.0	29.0	35.0
42	33.18525093664444	35.0	33.0	35.0	29.0	35.0
43	33.18956787657873	35.0	33.0	35.0	29.0	35.0
44	33.18586827012037	34.0	33.0	35.0	29.0	35.0
45	33.20969808903689	35.0	33.0	35.0	29.0	35.0
46	33.18776311919565	34.0	33.0	35.0	29.0	35.0
47	33.17951506107338	34.0	33.0	35.0	29.0	35.0
48	33.21789613394384	34.0	33.0	35.0	29.0	35.0
49	33.242172844367644	34.0	33.0	35.0	29.0	35.0
50	33.35322191700861	34.0	33.0	35.0	29.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	43.0
20	263.0
21	896.0
22	2509.0
23	6345.0
24	15414.0
25	35044.0
26	71273.0
27	133565.0
28	231549.0
29	371596.0
30	559590.0
31	803470.0
32	1133653.0
33	1665261.0
34	2564511.0
35	346984.0
36	206986.0
37	195178.0
38	175627.0
39	135531.0
40	48600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.52131258476808	18.85938421821495	30.61930319701697
2	25.534751970238613	27.140837521592342	26.868028623966627	20.45638188420242
3	29.88898648248859	19.535748076005053	23.23519527751656	27.3400701639898
4	22.56131306731067	21.48394483159551	26.970729770981844	28.98401233011198
5	24.446170363957744	23.800160732637515	25.44390758390353	26.309761319501213
6	25.81684406808676	25.207002152484602	23.15323197258239	25.822921806846246
7	32.28737249243011	23.544206358187914	20.237399463113928	23.931021686268046
8	28.748634950214818	20.919726168571657	25.601457738173544	24.730181143039985
9	27.989756310249607	23.70899465124522	21.945944890189967	26.355304148315206
10	29.04190595130111	21.908915491282926	23.774264280531877	25.274914276884086
11	30.32855979995163	21.763394434330838	24.17329253167691	23.734753234040625
12	27.869074707358028	20.356817263995026	24.220248520920805	27.553859507726138
13	26.869223491321986	21.286297686265744	23.462840486931423	28.38163833548084
14	26.8402939143912	22.302877045276855	21.860052309914124	28.996776730417817
15	23.618127286691763	24.048808033644708	24.164376982948436	28.16868769671509
16	26.16644617151287	25.532729887021844	24.388793752681988	23.912030188783298
17	28.40869518761428	22.280232011070904	19.815117254976077	29.495955546338738
18	27.81987834182283	22.240766921016395	20.36093036508368	29.5784243720771
19	26.288391576414927	21.454957809118792	24.298362974277605	27.958287640188672
20	29.129211692006855	20.2977517536689	23.639738301070956	26.933298253253284
21	29.461809913837428	23.70538707096076	22.101323602823793	24.73147941237802
22	26.688810009771487	22.567184002104803	24.376052330594522	26.367953657529185
23	26.94105340195395	23.75283709189966	22.506130875889472	26.799978630256916
24	29.17475452082085	24.25674942080528	22.486576412054603	24.081919646319264
25	25.460406059375153	24.683281157715104	23.292911819703473	26.56340096320627
26	27.600058213619334	23.668837510049908	22.632369616662366	26.098734659668395
27	27.53989537028928	23.431841519479008	24.848004461583322	24.18025864864839
28	28.188312666169747	22.18333232166396	23.87157848634802	25.75677652581827
29	28.48415376094895	21.275611007249587	25.48884356374495	24.751391668056506
30	25.932799362642427	22.866380390231193	26.202198182127933	24.998622064998447
31	27.34772794954436	21.466625200589608	27.95835019320172	23.227296656664308
32	27.12469973700804	21.756178277479236	25.165819565608277	25.95330241990445
33	24.539726057902083	22.559373442431617	26.59064368468037	26.310256814985927
34	24.270149053491817	22.037259458280932	29.896502684337634	23.79608880388962
35	28.14838791245026	22.295186768038494	23.35870501425398	26.197720305257256
36	24.99745475719887	24.393525011639788	22.93852073631517	27.670499494846172
37	23.754259154509302	25.20485254699481	25.632155479917216	25.408732818578667
38	28.22700608187404	24.88929090858542	21.163655122891374	25.720047886649166
39	25.105258487235865	25.67118138870752	24.671606238156055	24.551953885900556
40	25.824288970221094	23.939900518190814	22.49521822823186	27.74059228335623
41	25.31301564807943	24.581894153264475	22.927865809198973	27.177224389457123
42	24.705688985989465	24.601934589383728	25.903768286768898	24.788608137857914
43	23.246463345363072	26.37760855964071	24.49546976527753	25.880458329718692
44	22.379074442664606	25.38696895548395	24.45317316258543	27.78078343926601
45	25.15349316118323	22.117708708298878	27.852642761122244	24.876155369395647
46	24.321676551009695	23.26550516541069	23.947478752799046	28.46533953078057
47	27.352153752594553	22.24798088383031	23.469173180231437	26.930692183343695
48	23.438329782135643	23.60695770296876	24.56444533314336	28.390267181752243
49	27.11426825410396	22.431201599757284	21.885696340299024	28.568833805839738
50	23.56655989470783	26.247674788529462	23.720204165727825	26.46556115103489
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	231592.0
1	228735.0
2	122858.5
3	19759.0
4	19388.0
5	15172.5
6	7770.0
7	4452.0
8	4535.5
9	4348.0
10	4151.0
11	4067.0
12	4038.0
13	4020.0
14	4142.0
15	4428.0
16	4751.0
17	5438.0
18	6670.0
19	8900.5
20	12151.5
21	17116.0
22	24751.0
23	34741.5
24	44075.0
25	51365.0
26	57930.0
27	62580.5
28	65170.0
29	68627.0
30	73376.5
31	83265.5
32	96997.0
33	108280.5
34	122047.5
35	141577.5
36	158988.0
37	173093.5
38	189150.0
39	211572.5
40	245720.0
41	312288.0
42	402995.0
43	492423.5
44	527975.5
45	495710.5
46	472474.5
47	482141.5
48	523362.5
49	586601.5
50	673983.0
51	748573.0
52	808667.0
53	839846.0
54	809637.0
55	746316.0
56	677402.5
57	658455.5
58	669788.5
59	635317.5
60	575314.0
61	541348.5
62	515274.5
63	481901.5
64	444769.0
65	411592.0
66	370598.0
67	331152.5
68	297542.0
69	260677.0
70	226549.5
71	200396.0
72	182428.5
73	165551.0
74	140895.5
75	116265.5
76	99884.0
77	81016.5
78	64190.0
79	51594.5
80	39832.0
81	30172.5
82	22521.0
83	16605.5
84	12248.5
85	9224.0
86	6722.5
87	4576.5
88	3050.5
89	2266.0
90	1576.0
91	948.5
92	627.5
93	490.0
94	323.0
95	153.0
96	104.5
97	98.0
98	61.0
99	22.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	136675.0
25	129332.0
26	125144.0
27	124957.0
28	111328.0
29	93499.0
30	101819.0
31	111702.0
32	135344.0
33	98344.0
34	108704.0
35	109474.0
36	92254.0
37	103501.0
38	124045.0
39	126366.0
40	119588.0
41	114028.0
42	113405.0
43	110564.0
44	104909.0
45	124216.0
46	373491.0
47	870150.0
48	1467029.0
49	1685480.0
50	1788547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.12424674101944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.04351796347282	11.479578304727019
2	13.577481654709223	5.464731818820667
3	7.434726550878967	4.488548146856903
4	4.861432680953259	3.9133068314543613
5	3.5003378289120763	3.522083107297546
6	2.4737196317999257	2.9869046543067284
7	1.9368064601153554	2.7283739765073287
8	1.4776828835987756	2.37898039636183
9	1.1543688011812716	2.090772232659605
>10	5.908753692578539	20.487938548952442
>50	0.3412768440404925	4.7494110631784725
>100	0.23471705673285873	9.41033219598165
>500	0.028194063499637406	4.028667576765464
>1k	0.021841218379688	8.481915853507601
>5k	0.002783091538480794	3.9501444432440103
>10k+	0.0023595776087119774	9.838310849378484
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	55569	0.6384383083665416	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAA	36061	0.4143087663626457	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	36047	0.4141479188340393	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	35107	0.403348156199035	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	33551	0.3854711023053472	Illumina Single End Adapter 1 (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAA	33471	0.38455197357045323	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	33206	0.3815073596361169	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	30735	0.35311777083707924	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	29144	0.33483859812187533	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	27181	0.31228547678941443	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	26610	0.30572519544410864	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	22881	0.2628823072888632	Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22628	0.259975562664761	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	21354	0.24533843756157445	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	19475	0.2237504014007522	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	18187	0.2089524287689592	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	18158	0.20861924460256012	Illumina Single End Adapter 1 (100% over 32bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	18023	0.20706821486242652	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	17816	0.2046899692608884	Illumina Single End Adapter 1 (100% over 31bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	17451	0.2004964444079346	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	17338	0.19919817506989684	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCC	16272	0.18695078467743464	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	15775	0.18124069741190582	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	14626	0.1680397109569911	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	14460	0.1661325188320861	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	13619	0.15647017800651317	Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	13481	0.15488468093882107	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13440	0.1544136274621879	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	13348	0.15335662941705983	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	13304	0.15285110861286813	Illumina Single End Adapter 1 (100% over 31bp)
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	13071	0.15017414617248945	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	12849	0.14762356393315865	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGG	12808	0.1471525104565255	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	12507	0.14369428859148692	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA	12157	0.13967310037632577	Illumina Single End Adapter 1 (100% over 32bp)
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	11958	0.137386767648277	No Hit
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	11826	0.13587020523570192	No Hit
AGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	11419	0.13119413779692884	Illumina Single End Adapter 2 (100% over 34bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	11124	0.12780485058700733	Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGGA	9686	0.11128351157728811	No Hit
AAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	9613	0.11044480660669735	Illumina Single End Adapter 2 (100% over 34bp)
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	9308	0.10694062830491408	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	9229	0.10603298867920626	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTG	8801	0.10111564994752349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.5475249873763413	0.0	0.0	0.0	0.0
2	1.305001956020839	0.0	0.0	0.0	0.0
3	1.6866127176396315	0.0	0.0	0.0	0.0
4	2.019888796912187	0.0	0.0	0.0	0.0
5	2.445387955622167	0.0	0.0	0.0	0.0
6	2.6218376945034376	0.0	0.0	0.0	0.0
7	2.745219238053768	0.0	0.0	0.0	0.0
8	2.8573874110383914	0.0	0.0	0.0	0.0
9	2.9608123719323363	0.0	0.0	0.0	6.893465511704818E-5
10	3.0408914629599737	0.0	0.0	0.0	6.893465511704818E-5
11	3.1280823125738535	0.0	0.0	0.0	6.893465511704818E-5
12	3.187756745686845	0.0	0.0	0.0	6.893465511704818E-5
13	3.2386649884907848	0.0	0.0	0.0	6.893465511704818E-5
14	3.2980292156557494	3.4467327558524086E-4	0.0	0.0	6.893465511704818E-5
15	3.382589059265995	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
16	3.4416086131553745	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
17	3.4960899689162153	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
18	3.564415701246396	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
19	3.725412588272262	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
20	3.774677888462579	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
21	3.859180286526894	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
22	3.898450061725239	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
23	3.943027805367597	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
24	3.9780925666038023	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
25	4.032861150094297	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
26	4.148073937013256	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
27	4.1815302229633975	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
28	4.214595879201208	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
29	4.244950105671082	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
30	4.2729720429761615	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
31	4.2988110495358685	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
32	4.322168408511362	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
33	4.3434692169425295	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
34	4.3612773361811	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
35	4.377902077173495	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
36	4.392619626040985	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
37	4.403890442152623	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
38	4.411415808669567	4.250970398884637E-4	0.0	0.0	8.042376430322287E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	4290	0.0	130.10017	44
CTATACG	685	0.0	117.57006	44
CGTGCGC	3505	0.0	104.73382	44
AATCGCA	285	0.0	82.145546	44
TTAAGCC	3685	0.0	79.040344	43
TCGTGCG	6000	0.0	77.75055	43
ATGTTCT	2205	0.0	76.445656	44
AATCCCG	4950	0.0	73.527115	43
CCAGAAT	2005	0.0	70.05929	44
CTATCAT	1800	0.0	69.19393	44
TATACGC	280	0.0	66.889946	44
CGCTACC	1160	0.0	62.16152	44
TTTGGAC	3765	0.0	60.48213	43
ACTTCTA	1580	0.0	60.454964	44
TTCAGGA	3240	0.0	59.251278	44
ATTAAGC	5190	0.0	58.38969	42
AATCCTG	3025	0.0	57.1347	43
TGTGTTA	3810	0.0	54.388477	42
CTATGCG	315	0.0	53.51196	44
TAATCCC	10330	0.0	52.706745	42
>>END_MODULE